Table of Contents
Biology, Volume 9, Issue 5 (May 2020) – 24 articles
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Cover Story (view full-size image) 5-FU-based chemotherapy remains the mainstay for colorectal cancer (CRC) treatment. However, cancer [...] Read more. 5-FU-based chemotherapy remains the mainstay for colorectal cancer (CRC) treatment. However, cancer cells acquire chemoresistance, causing treatment failure. Understanding the molecular mechanism of resistance will uncover new avenues for curing CRC. One mechanism of acquired chemoresistance is defective drug metabolism. 5-FU is converted into various metabolites, causing DNA and RNA damage and inducing apoptosis. An HRMS-based method was used to extract and quantify levels of 5-FU metabolites from parental and resistant CRC cell lysates and media. Results show the reduced levels of 5-FU metabolites in resistant cells. Moreover, treatment of CRC cells with FdUMP, an active metabolite of 5-FU, induced resistant cell death. Overall, an effective and efficient protocol was developed for the comparative quantitation of polar compounds from cells. FdUMP is also highlighted as an alternative CRC therapy. View this paper.