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Biosensors, Volume 7, Issue 4

2017 December - 23 articles

Cover Story: A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate, or the sample, in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB, which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples. View this paper
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Articles (23)

  • Review
  • Open Access
27 Citations
8,874 Views
12 Pages

20 December 2017

Food pathogens contaminate food products that allow their growth on the shelf and also under refrigerated conditions. Therefore, it is of utmost importance to lower the limit of detection (LOD) of the method used and to obtain the results within hour...

  • Editorial
  • Open Access
197 Citations
13,248 Views
4 Pages

18 December 2017

Recent years have witnessed tremendous advances in point-of-care diagnostics (POCD), which are a result of continuous developments in biosensors, microfluidic, bioanalytical platforms, assay formats, lab-on-a-chip technologies, and complementary tech...

  • Review
  • Open Access
96 Citations
12,903 Views
14 Pages

Fluorescent and Colorimetric Electrospun Nanofibers for Heavy-Metal Sensing

  • Idelma A. A. Terra,
  • Luiza A. Mercante,
  • Rafaela S. Andre and
  • Daniel S. Correa

15 December 2017

The accumulation of heavy metals in the human body and/or in the environment can be highly deleterious for mankind, and currently, considerable efforts have been made to develop reliable and sensitive techniques for their detection. Among the detecti...

  • Article
  • Open Access
2 Citations
5,302 Views
9 Pages

Spontaneous Charge Generation in Flowing Albumin Solutions at 35 °C and 38 °C

  • Yuri D. Ivanov,
  • Andrey F. Kozlov,
  • Rafael A. Galiullin,
  • Ekaterina F. Kolesanova and
  • Tatyana O. Pleshakova

11 December 2017

The time dependence of a charge accumulation in a 10−15 M albumin solution, flowing through a measuring cell of an analytical flow system injector, had a nonlinear character under certain conditions, within a human physiological temperature range. Sh...

  • Feature Paper
  • Review
  • Open Access
54 Citations
14,009 Views
39 Pages

7 December 2017

Biomimetic cross-reactive sensor arrays (B-CRSAs) have been used to detect and diagnose a wide variety of diseases including metabolic disorders, mental health diseases, and cancer by analyzing both vapor and liquid patient samples. Technological adv...

  • Article
  • Open Access
14 Citations
7,174 Views
14 Pages

An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

  • Francesca Costantini,
  • Roald M. Tiggelaar,
  • Riccardo Salvio,
  • Marco Nardecchia,
  • Stefan Schlautmann,
  • Cesare Manetti,
  • Han J. G. E. Gardeniers,
  • Giampiero De Cesare,
  • Domenico Caputo and
  • Augusto Nascetti

5 December 2017

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorph...

  • Article
  • Open Access
29 Citations
7,580 Views
16 Pages

Study of SH-SY5Y Cancer Cell Response to Treatment with Polyphenol Extracts Using FT-IR Spectroscopy

  • Valerio Ricciardi,
  • Marianna Portaccio,
  • Simona Piccolella,
  • Lorenzo Manti,
  • Severina Pacifico and
  • Maria Lepore

30 November 2017

Plant polyphenols are important components of human diet and a number of them are considered to possess chemo-preventive and therapeutic properties against cancer. They are recognized as naturally occurring antioxidants, but also as pro-oxidant, pro-...

  • Article
  • Open Access
12 Citations
7,894 Views
15 Pages

28 November 2017

Monitoring food safety is essential for protecting the health and safety of consumers. Conventional methods used are time consuming and laborious, requiring anywhere from three to seven days to obtain results. Thus, better monitoring methods are requ...

  • Feature Paper
  • Article
  • Open Access
116 Citations
16,202 Views
29 Pages

24 November 2017

Continuous in-home monitoring of older adults living alone aims to improve their quality of life and independence, by detecting early signs of illness and functional decline or emergency conditions. To meet requirements for technology acceptance by s...

  • Feature Paper
  • Article
  • Open Access
15 Citations
7,495 Views
16 Pages

21 November 2017

Electric fields have been studied extensively in biomedical engineering (BME) for numerous regenerative therapies. Recent studies have begun to examine the biological effects of electric fields in combination with other environmental cues, such as ti...

  • Feature Paper
  • Article
  • Open Access
2 Citations
5,363 Views
10 Pages

17 November 2017

Somatostatin (SRIF) is widely distributed throughout the body, and regulates the endocrine system via interactions with various hormones, including the pituitary growth hormone, the thyroid stimulating hormone and the majority of the hormones of the...

  • Review
  • Open Access
33 Citations
8,330 Views
15 Pages

15 November 2017

Modern instrumentation for Raman microspectroscopy and current techniques in analysis of spectral data provide new opportunities to study molecular interactions and dynamics at subcellular levels in biological systems. Implementation of biomolecular...

  • Article
  • Open Access
38 Citations
15,886 Views
11 Pages

An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine

  • Gonzalo Martínez-García,
  • Elena Pérez-Julián,
  • Lourdes Agüí,
  • Naomí Cabré,
  • Jorge Joven,
  • Paloma Yáñez-Sedeño and
  • José Manuel Pingarrón

11 November 2017

A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-...

  • Review
  • Open Access
119 Citations
25,812 Views
15 Pages

31 October 2017

Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared wi...

  • Article
  • Open Access
23 Citations
12,063 Views
20 Pages

Encapsulation-Stabilized, Europium Containing Nanoparticle as a Probe for Time-Resolved luminescence Detection of Cardiac Troponin I

  • Ka Ram Kim,
  • Yong Duk Han,
  • Hyeong Jin Chun,
  • Kyung Won Lee,
  • Dong-Ki Hong,
  • Kook-Nyung Lee and
  • Hyun C. Yoon

18 October 2017

The use of a robust optical signaling probe with a high signal-to-noise ratio is important in the development of immunoassays. Lanthanide chelates are a promising material for this purpose, which provide time-resolved luminescence (TRL) due to their...

  • Article
  • Open Access
65 Citations
8,874 Views
11 Pages

15 October 2017

A biosensor based on chitosan-gelatin composite biopolymers nanofibers is found to be effective for the immobilization of horseradish peroxidase to detect hydrogen peroxide. The biopolymer nanofibers were fabricated by an electrospining technique. Up...

  • Review
  • Open Access
19 Citations
8,894 Views
19 Pages

Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform

  • Georgios Koukouvinos,
  • Panagiota Petrou,
  • Dimitrios Goustouridis,
  • Konstantinos Misiakos,
  • Sotirios Kakabakos and
  • Ioannis Raptis

13 October 2017

The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determi...

  • Article
  • Open Access
5 Citations
8,061 Views
13 Pages

CFD Modeling of Chamber Filling in a Micro-Biosensor for Protein Detection

  • Meiirbek Islamov,
  • Marzhan Sypabekova,
  • Damira Kanayeva and
  • Luis Rojas-Solórzano

3 October 2017

Tuberculosis (TB) remains one of the main causes of human death around the globe. The mortality rate for patients infected with active TB goes beyond 50% when not diagnosed. Rapid and accurate diagnostics coupled with further prompt treatment of the...

  • Feature Paper
  • Article
  • Open Access
11 Citations
13,383 Views
11 Pages

30 September 2017

In this study, we describe a microbead-based method using dielectrophoresis (DEP) for the fast detection of DNA amplified by polymerase chain reaction (PCR). This electrical method measures the change in impedance caused by DEP-trapped microbeads to...

  • Article
  • Open Access
43 Citations
9,164 Views
12 Pages

Raman Computational and Experimental Studies of Dopamine Detection

  • John D. Ciubuc,
  • Kevin E. Bennet,
  • Chao Qiu,
  • Matthew Alonzo,
  • William G. Durrer and
  • Felicia S. Manciu

28 September 2017

A combined theoretical and experimental analysis of dopamine (DA) is presented in this work with the objective of achieving more accurate detection and monitoring of this neurotransmitter at very low concentrations, specific to physiological levels....

  • Review
  • Open Access
31 Citations
11,207 Views
18 Pages

A Review on Passive and Integrated Near-Field Microwave Biosensors

  • Subhajit Guha,
  • Farabi Ibne Jamal and
  • Christian Wenger

23 September 2017

In this paper we review the advancement of passive and integrated microwave biosensors. The interaction of microwave with biological material is discussed in this paper. Passive microwave biosensors are microwave structures, which are fabricated on a...

  • Article
  • Open Access
7 Citations
7,792 Views
11 Pages

21 September 2017

Herein we demonstrate the sensitive nature of human blood-brain barrier (BBB) endothelial cells to sodium azide and its gaseous product. Sodium azide is known to be acutely cytotoxic at low millimolar concentrations, hence its use as a biological pre...

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Biosensors - ISSN 2079-6374