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Biosensors, Volume 7, Issue 4

December 2017 - 23 articles

Cover Story: A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate, or the sample, in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB, which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples. View this paper
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Articles (23)

  • Review
  • Open Access
27 Citations
8,761 Views
12 Pages

20 December 2017

Food pathogens contaminate food products that allow their growth on the shelf and also under refrigerated conditions. Therefore, it is of utmost importance to lower the limit of detection (LOD) of the method used and to obtain the results within hour...

  • Editorial
  • Open Access
193 Citations
12,854 Views
4 Pages

18 December 2017

Recent years have witnessed tremendous advances in point-of-care diagnostics (POCD), which are a result of continuous developments in biosensors, microfluidic, bioanalytical platforms, assay formats, lab-on-a-chip technologies, and complementary tech...

  • Review
  • Open Access
94 Citations
12,701 Views
14 Pages

Fluorescent and Colorimetric Electrospun Nanofibers for Heavy-Metal Sensing

  • Idelma A. A. Terra,
  • Luiza A. Mercante,
  • Rafaela S. Andre and
  • Daniel S. Correa

15 December 2017

The accumulation of heavy metals in the human body and/or in the environment can be highly deleterious for mankind, and currently, considerable efforts have been made to develop reliable and sensitive techniques for their detection. Among the detecti...

  • Article
  • Open Access
2 Citations
5,244 Views
9 Pages

Spontaneous Charge Generation in Flowing Albumin Solutions at 35 °C and 38 °C

  • Yuri D. Ivanov,
  • Andrey F. Kozlov,
  • Rafael A. Galiullin,
  • Ekaterina F. Kolesanova and
  • Tatyana O. Pleshakova

11 December 2017

The time dependence of a charge accumulation in a 10−15 M albumin solution, flowing through a measuring cell of an analytical flow system injector, had a nonlinear character under certain conditions, within a human physiological temperature range. Sh...

  • Feature Paper
  • Review
  • Open Access
53 Citations
13,631 Views
39 Pages

7 December 2017

Biomimetic cross-reactive sensor arrays (B-CRSAs) have been used to detect and diagnose a wide variety of diseases including metabolic disorders, mental health diseases, and cancer by analyzing both vapor and liquid patient samples. Technological adv...

  • Article
  • Open Access
14 Citations
7,085 Views
14 Pages

An All-Glass Microfluidic Network with Integrated Amorphous Silicon Photosensors for on-Chip Monitoring of Enzymatic Biochemical Assay

  • Francesca Costantini,
  • Roald M. Tiggelaar,
  • Riccardo Salvio,
  • Marco Nardecchia,
  • Stefan Schlautmann,
  • Cesare Manetti,
  • Han J. G. E. Gardeniers,
  • Giampiero De Cesare,
  • Domenico Caputo and
  • Augusto Nascetti

5 December 2017

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorph...

  • Article
  • Open Access
29 Citations
7,485 Views
16 Pages

Study of SH-SY5Y Cancer Cell Response to Treatment with Polyphenol Extracts Using FT-IR Spectroscopy

  • Valerio Ricciardi,
  • Marianna Portaccio,
  • Simona Piccolella,
  • Lorenzo Manti,
  • Severina Pacifico and
  • Maria Lepore

30 November 2017

Plant polyphenols are important components of human diet and a number of them are considered to possess chemo-preventive and therapeutic properties against cancer. They are recognized as naturally occurring antioxidants, but also as pro-oxidant, pro-...

  • Article
  • Open Access
12 Citations
7,730 Views
15 Pages

28 November 2017

Monitoring food safety is essential for protecting the health and safety of consumers. Conventional methods used are time consuming and laborious, requiring anywhere from three to seven days to obtain results. Thus, better monitoring methods are requ...

  • Feature Paper
  • Article
  • Open Access
116 Citations
15,790 Views
29 Pages

24 November 2017

Continuous in-home monitoring of older adults living alone aims to improve their quality of life and independence, by detecting early signs of illness and functional decline or emergency conditions. To meet requirements for technology acceptance by s...

  • Feature Paper
  • Article
  • Open Access
15 Citations
7,370 Views
16 Pages

21 November 2017

Electric fields have been studied extensively in biomedical engineering (BME) for numerous regenerative therapies. Recent studies have begun to examine the biological effects of electric fields in combination with other environmental cues, such as ti...

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Biosensors - ISSN 2079-6374