Biosensors, Volume 16, Issue 2

Fumonisin B1 (FB1) is a secondary metabolite produced by Fusarium species, exhibiting strong toxicity and classified as a Group 2B carcinogen by the International Agency for Research on Cancer. It poses a significant threat to both human and animal h...

Lateral flow assays (LFAs) are among the most successful technologies for point-of-care and at-home testing, but further advances are needed to reduce costs and accelerate development. Alpaca-derived nanobodies (Nbs), single-domain antibody fragments...

Filamentous fungi are increasingly recognized as versatile biological platforms for the development of advanced (bio)sensing technologies, owing to their extensive secretory capacity, material-forming ability, and intrinsic bioelectrical activity. Th...

Hexavalent chromium (Cr(VI)) is a high-priority environmental pollutant due to its strong oxidizing properties, which cause DNA damage and other severe health effects. Conventional detection methods are often costly and lack real-time monitoring capa...

Early detection of cancer biomarkers in blood is critical for improving patient outcomes; however, conventional immunoassays often rely on complex instrumentation and are not well suited for point-of-care testing or multiplexed analysis. Herein, we p...

The global expansion of genetically modified (GM) crop cultivation has increased the demand for analytical platforms that can provide rapid, reliable, and cost-effective detection of GM-derived ingredients to support traceability, regulatory complian...

Background: Brain temperature is an important determinant of neurological outcomes in ill infants, yet contributions of environmental temperature and cerebral blood flow remain uncovered because of the lack of non-invasive probes. Methods: Using non-...

Microfluidics-based preparation methods for cell-laden hydrogel microspheres are well-suited for large-scale comparative analysis of single or few cells. However, in existing studies, the preparation of cell-laden hydrogel microspheres and the cell c...

The sensitive detection of microRNA-21 (miR-21), a key biomarker for various cancers, is crucial for early diagnosis, yet conventional methods often face limitations in sensitivity and operational complexity. Here, we report a label-free biosensor ba...

We report a highly sensitive and interference-free electrochemical sensor for dopamine (DA) detection in the presence of uric acid (UA) and ascorbic acid (AA), based on an in situ deposited graphitic carbon nitride (g-C₃N₄) and polymethyl thymol blue...

Dynamic monitoring of hemostatic equilibrium is indispensable for clinical safety in high-risk scenarios, while current clinical methods are limited by sample volume, detection speed, and physiological relevance. These shortcomings underscore the dem...

Culturing cells and micro-tissue samples in 3D bio-scaffolding structures is gaining popularity; however, precise control of tissue micro-environment in such systems remains challenging. We describe a family of new hybrid bio-scaffolds with 3D O₂-sen...

Severe Dengue fever can cause Dengue Hemorrhagic Fever (DHF), a life-threatening condition characterized by plasma leakage and hemoconcentration. A hematocrit (Hct) rise of ≥20% is a key indicator for medical intervention, but current monitoring i...

Traditional sample preparation for flow cytometry is often labor-intensive, operator-dependent, and reagent-consuming, limiting its suitability for automated and point-of-care biosensing applications. To address these challenges, this study presents...

Correct focal positioning is essential for microscopy imaging of live moving subjects such as Caenorhabditis elegans. However, many methods can be too slow to perform real-time control to keep the subject in focus. In this work, we propose a convolut...

Avian influenza (AI), particularly highly pathogenic avian influenza (HPAI), represents a serious and growing threat to global poultry production, international trade, and human health security. Control of AI is complicated by the high evolutionary r...

Over the past decade, clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins, originally identified as adaptive immune systems in bacteria and archaea that defend against invading nucleic acids, have r...

Lysophosphatidic acid (LPA) is a cell-signaling lipid that has been proposed as an early-stage biomarker for ovarian cancer (OC). Diagnosing OC in Stage I is critical to improving patient outcomes, increasing the survival rate from 30% (when diagnose...

This study aims to develop an innovative electrochemical genosensor based on activated biochar (ABC) derived from the biomass of the seaweed Sargassum spp. The synthesis process begins with the pyrolysis of Sargassum spp. at 500 °C to obtain bioc...

With the increasing emphasis on food safety and health, it has become particularly important to develop rapid, sensitive and low-cost detection methods for foodborne pathogens. Lateral flow assay (LFA) has shown great potential in the field of point-...

Early diagnosis of Alzheimer’s disease represents a critical clinical challenge, and the high-sensitive biomarkers measurement holds great potential for enabling early identification and intervention. This study proposes an electrochemical immu...

Electrochemical biosensors have reached a high degree of analytical maturity; however, only a small portion of laboratory demonstrations actually progress to commercial products. In this review, we looked non-analytically at the factors which are in...

This study addresses the need for detecting human papillomavirus type 16 DNA (HPV-16), a high-risk factor for cervical cancer, by developing a highly sensitive fluorescence sensing method based on tungsten disulfide (WS₂) nanosheets and exonuclease I...

The accurate monitoring and dynamic analysis of metal ions are of considerable practical significance in environmental toxicology and life sciences. Colorimetric analysis and surface-enhanced Raman scattering (SERS) sensing technologies, utilizing th...

This work presents the development of an automated and portable monitoring system for the point-of-need detection of tebuconazole and lambda-cyhalothrin. The system features nanoparticle/aptamer-modified electrochemical sensors that are integrated in...

Microneedle array (MNA) electrodes have garnered significant attention for their capacity to record high-fidelity surface bioelectrical signals over extended periods and convenience. However, accuracy limitations in 3D-printed metal MNA electrodes, p...

Considering the present environmental concerns, nanomaterial-based methods should be applied to achieve the bioeconomic sustainability initiatives and climate change mitigation. Plants and plant extracts are one of the most underused biomass and bioa...

Forensic DNA profiling commonly relies on polymerase chain reaction (PCR) amplification followed by capillary electrophoresis (CE) or massively parallel sequencing (MPS), which requires expensive, laboratory-based equipment that depends on a stable p...

Rare-earth-doped upconversion nanoparticles (UCNPs) exhibit upconversion luminescence upon excitation with infrared light and have been extensively utilized in the field of biosensing. In this study, a UCNPs-based biosensor with porous silicon (PSi)...

Microfluidic chip technologies, also known as lab-on-a-chip systems, have profoundly transformed laboratory medicine by enabling the miniaturization, automation, and rapid processing of complex diagnostic assays using minimal sample volumes. Recent a...

Sulfamethazine (SMZ) is widely used in livestock production, and its residues can enter water and soil environments, posing potential risks to human health and ecosystems. This study focuses on environmental samples and constructs an AuNP-based color...

In on-site rapid detection, the electrochemical method boasts high sensitivity and rapid response capabilities, while the colorimetric method can provide intuitive visual readings suitable for on-site screening. Therefore, this study developed an inn...

Accurate identification and characterization of subcutaneous tumors are essential for improving breast tumor detection and treatment. This study introduces an innovative Tactile-Sensation Imaging System (TSIS) designed, implemented, and tested to det...

Lateral flow immunoassays (LFAs) are widely used for on-site testing; however, their use for the rapid detection of plant viruses in the field is often limited by inconvenient sample preparation. Here, we present a new sampling method and a simplifie...

The ultrasensitive detection of microRNA-17 (miRNA-107) is required for clinical diagnosis. In this work, an aggregation-induced electrochemiluminescence (AIECL) sensor was developed for the quantification of miRNA-107, in which AIECL-active polymer...

The increasing impact of infectious, cardiovascular and neurodegenerative diseases has intensified the demand for early and decentralized diagnostics. Label-free electrochemical biosensors are promising candidates, offering high sensitivity, low reag...

Background: Artificial Intelligence (AI) and Machine Learning (ML) are increasingly integrated into sport and exercise through wearable biosensing systems that enable continuous monitoring and data-driven training adaptation. However, their practical...

Cerium oxide (CeO₂) nanozymes, also known as nanoceria have emerged as a versatile class of catalytic nanomaterials capable of mimicking key redox enzymes, including oxidases and peroxidases. Their tunable Ce³⁺/Ce⁴⁺ redox cycling, high density of oxy...

The demand for rapid and highly sensitive sensing technologies is increasing across diverse fields, including precise disease diagnosis, early-stage screening, and real-time environmental monitoring. Field-effect transistor (FET)-based sensing platfo...

Temperate fruits, mostly comprising pome, stone fruits, and berries with immense nutritional benefits and a storehouse of various therapeutic phytochemicals, are prone to several physiological disorders immediately after harvest. The etiology, sympto...

The growing global population and the rapid increase in older adults are driving healthcare costs upward. In response, the healthcare system is shifting toward models that enable continuous monitoring of individuals without requiring hospital admissi...

The precise and reliable detection of milk adulterants has garnered increased scientific interest owing to the rising incidence of food fraud. Recent years have witnessed substantial advancements in optical and electrochemical biosensors for the quic...

Due to an error in the original publication [...]

Tetrodotoxin (TTX) is a potent marine neurotoxin, necessitating sensitive and user-friendly on-site assays. To address long workflows of traditional immunoassays and limited signal amplification in colorimetric microfluidics, we developed a nanozyme-...

Fluorescence imaging is crucial for providing detailed information in clinical practice. However, traditional first near-infrared (NIR-I) dyes such as indocyanine green (ICG) exhibit limitations such as shallow penetration depth, low contrast, and su...

Acoustic bubbles are emerging as powerful microscale sensors that convert local biochemical and biomechanical cues into measurable signals in a remote, label-free, and clinically compatible manner. Originally developed as vascular contrast agents, mi...

Advanced glycation end products (AGEs) are important biomarkers associated with diabetes and metabolic disorders; yet existing detection methods are invasive and unsuitable for frequent monitoring. This study aimed to develop a non-invasive and porta...

Microfluidics has emerged as a powerful platform for the analysis of minute sample volumes, driving its widespread adoption in biosensing applications. Optical imaging and electrochemical sensing are two typical integration strategies, each offering...

We report a novel dual-receptor lateral flow biosensor (LFB) for the rapid, sensitive, and visual detection of MCF-7 breast cancer cells as a model for circulating tumor cells (CTCs). The biosensor employs a MUC1-specific aptamer conjugated to colloi...

Taste masking in pharmaceutical products is a complex and subjective process that requires reliable evaluation methods. This review focuses on the electronic tongue (e-tongue), an emerging sensor-based technology designed to mimic human taste percept...