Antioxidants

17 pages, 656 KiB

Open AccessReview

The Role of Nicotinamide Adenine Dinucleotide Phosphate Oxidases in Lung Architecture Remodeling

by Anantha Harijith¹, Viswanathan Natarajan^2,3

and Panfeng Fu^3,*

¹ Department of Pediatric, University of Illinois at Chicago, Chicago, IL 60612, USA

² Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA

³ Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA

Antioxidants 2017, 6(4), 104; https://doi.org/10.3390/antiox6040104 - 19 Dec 2017

Cited by 28 | Viewed by 7138

Abstract

Chronic lung disorders, such as pulmonary artery hypertension (PAH), chronic obstructive pulmonary disease (COPD), asthma and neonatal bronchopulmonary dysplasia (BPD), are characterized by airway and/or vascular remodeling. Despite differences in the pathology, reactive oxygen species (ROS) have been highlighted as a critical contributor [...] Read more.

Chronic lung disorders, such as pulmonary artery hypertension (PAH), chronic obstructive pulmonary disease (COPD), asthma and neonatal bronchopulmonary dysplasia (BPD), are characterized by airway and/or vascular remodeling. Despite differences in the pathology, reactive oxygen species (ROS) have been highlighted as a critical contributor to the initiation and development of airway and vascular remodeling. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (Nox) appear to play a pivotal role in lung signaling, leading to marked changes in pulmonary airway and vascular cell phenotypes, including proliferation, hypertrophy and apoptosis. In this review, we summarized the current literature regarding the role of Nox in the airway and vascular remodeling. Full article

(This article belongs to the Special Issue ROS Derived from NADPH Oxidase (NOX) in Angiogenesis)

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18 pages, 1950 KiB

Open AccessArticle

Co-Enzyme Q10 and n-3 Polyunsaturated Fatty Acid Supplementation Reverse Intermittent Hypoxia-Induced Growth Restriction and Improved Antioxidant Profiles in Neonatal Rats

by Kay D. Beharry^1,2,3,*, Charles L. Cai¹, Michael M. Henry¹, Sara Chowdhury¹, Gloria B. Valencia¹ and Jacob V. Aranda^1,2,3

¹ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USA

² Department of Ophthalmology; State University of New York, Downstate Medical Center, Brooklyn, NY 11203, USA

³ SUNY Eye Institute, State University of New York, New York, NY 10062, USA

Antioxidants 2017, 6(4), 103; https://doi.org/10.3390/antiox6040103 - 16 Dec 2017

Cited by 19 | Viewed by 5628

Abstract

Neonatal intermittent hypoxia (IH) increases the risk for many morbidities in extremely low birth weight/gestational age (ELBW/ELGA) neonates with compromised antioxidant systems and poor growth. We hypothesized that supplementation with coenzyme Q10 (CoQ10, ubiquinol) or n-3 polyunsaturated fatty acids (PUFAs) during neonatal [...] Read more.

Neonatal intermittent hypoxia (IH) increases the risk for many morbidities in extremely low birth weight/gestational age (ELBW/ELGA) neonates with compromised antioxidant systems and poor growth. We hypothesized that supplementation with coenzyme Q10 (CoQ10, ubiquinol) or n-3 polyunsaturated fatty acids (PUFAs) during neonatal IH improves antioxidant profiles and somatic growth in neonatal rats. Newborn rats were exposed to two IH paradigms at birth (P0): (1) 50% O₂ with brief hypoxic episodes (12% O₂); or (2) room air (RA) with brief hypoxia, until P14 during which they received daily oral CoQ10 in olive oil, n-3 PUFAs in fish oil, or olive oil only from P0 to P14. Pups were studied at P14 or placed in RA until P21 for recovery from IH (IHR). Body weight and length; organ weights; and serum antioxidants and growth factors were determined at P14 and P21. Neonatal IH resulted in sustained reductions in somatic growth, an effect that was reversed with n-3 PUFAs. Improved growth was associated with higher serum growth factors. CoQ10 decreased superoxide dismutase (SOD) and glutathione, but increased catalase, suggesting reduced oxidative stress. Further studies are needed to determine the synergistic effects of CoQ10 and n-3 PUFA co-administration for the prevention of IH-induced oxidative stress and postnatal growth deficits. Full article

(This article belongs to the Special Issue Antioxidants: Infant Nutrition)

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20 pages, 5272 KiB

Open AccessArticle

In Vitro and In Vivo Antioxidant and Anti-Hyperglycemic Activities of Moroccan Oat Cultivars

by Ilias Marmouzi^1,*

, El Mostafa Karym², Nezha Saidi³, Bouchra Meddah¹, Mourad Kharbach^4,5

, Azlarab Masrar⁶, Mounya Bouabdellah⁶, Layachi Chabraoui⁶, Khalid El Allali⁷, Yahia Cherrah¹ and My El Abbes Faouzi¹

¹ Laboratoire de Pharmacologie et Toxicologie, équipe de Pharmacocinétique, Faculté de Médicine et Pharmacie, University Mohammed V in Rabat, BP 6203, Rabat Instituts, Rabat 10100, Morocco

² Laboratoire de Biochimie et Neurosciences, FST, Université Hassan I, BP 577, Settat 26000, Morocco

³ Regional Office of Rabat, National Institute for Agricultural Research, P.O. Box 6570, Rabat Institutes, Rabat 10101, Morocco

⁴ Pharmaceutical and Toxicological Analysis Research Team, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V, Rabat 10100, Morocco

⁵ Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, CePhaR, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium

⁶ Central Laboratory of Biochemistry, Ibn Sina Hospital, Rabat 10100, Morocco

⁷ Comparative Anatomy Unit-URAC-49, Hassan II Agronomy and Veterinary Institute, Rabat 10101, Morocco

Antioxidants 2017, 6(4), 102; https://doi.org/10.3390/antiox6040102 - 6 Dec 2017

Cited by 25 | Viewed by 7171

Abstract

Improvement of oat lines via introgression is an important process for food biochemical functionality. This work aims to evaluate the protective effect of phenolic compounds from hybrid Oat line (F11-5) and its parent (Amlal) on hyperglycemia-induced oxidative stress and to establish the possible [...] Read more.

Improvement of oat lines via introgression is an important process for food biochemical functionality. This work aims to evaluate the protective effect of phenolic compounds from hybrid Oat line (F11-5) and its parent (Amlal) on hyperglycemia-induced oxidative stress and to establish the possible mechanisms of antidiabetic activity by digestive enzyme inhibition. Eight phenolic acids were quantified in our samples including ferulic, p-hydroxybenzoic, caffeic, salicylic, syringic, sinapic, p-coumaric and chlorogenic acids. The Oat extract (2000 mg/kg) ameliorated the glucose tolerance, decreased Fasting Blood Glucose (FBG) and oxidative stress markers, including Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione (GSH) and Malondialdehyde (MDA) in rat liver and kidney. Furthermore, Metformin and Oat intake prevented anxiety, hypercholesterolemia and atherosclerosis in diabetic rats. In vivo anti-hyperglycemic effect of Oat extracts has been confirmed by their inhibitory activities on α-amylase (723.91 μg/mL and 1027.14 μg/mL) and α-glucosidase (1548.12 μg/mL & 1803.52 μg/mL) enzymes by mean of a mixed inhibition. Full article

(This article belongs to the Special Issue Dietary Antioxidants and Prevention of Non-Communicable Diseases)

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16 pages, 900 KiB

Open AccessArticle

Tempol Supplementation Restores Diaphragm Force and Metabolic Enzyme Activities in mdx Mice

by David P. Burns, Izza Ali, Clement Rieux, James Healy, Greg Jasionek and Ken D. O’Halloran^*

Department of Physiology, University College Cork, Western Gateway Building, Western Road, Cork T12 XF62, Ireland

Antioxidants 2017, 6(4), 101; https://doi.org/10.3390/antiox6040101 - 6 Dec 2017

Cited by 22 | Viewed by 5968

Abstract

Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of [...] Read more.

Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of dystrophin-deficient diaphragm muscle. Diaphragm muscle function was assessed, ex vivo, in adult male wild-type and dystrophin-deficient mdx mice, with and without a 14-day antioxidant intervention. The enzymatic activities of muscle citrate synthase, phosphofructokinase, and lactate dehydrogenase were assessed using spectrophotometric assays. Dystrophic diaphragm displayed mechanical dysfunction and altered biochemical status. Chronic tempol supplementation in the drinking water increased diaphragm functional capacity and citrate synthase and lactate dehydrogenase enzymatic activities, restoring all values to wild-type levels. Chronic supplementation with tempol recovers force-generating capacity and metabolic enzyme activity in mdx diaphragm. These findings may have relevance in the search for therapeutic strategies in neuromuscular disease. Full article

(This article belongs to the Special Issue Exercise Induced Muscle Damage and Oxidative Stress)

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13 pages, 2481 KiB

Open AccessArticle

Effects of the Macular Carotenoid Lutein in Human Retinal Pigment Epithelial Cells

by Xiaoming Gong¹

, Christian S. Draper², Geoffrey S. Allison², Raju Marisiddaiah³ and Lewis P. Rubin^1,4,*

¹ Department of Pediatrics, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA

² Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA

³ All Children’s Research Institute, St. Petersburg, FL 33701, USA

⁴ Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA

Antioxidants 2017, 6(4), 100; https://doi.org/10.3390/antiox6040100 - 4 Dec 2017

Cited by 66 | Viewed by 8894

Abstract

Retinal pigment epithelial (RPE) cells are central to retinal health and homoeostasis. Oxidative stress-induced damage to the RPE occurs as part of the pathogenesis of age-related macular degeneration and neovascular retinopathies (e.g., retinopathy of prematurity, diabetic retinopathy). The xanthophyll carotenoids, lutein and zeaxanthin, [...] Read more.

Retinal pigment epithelial (RPE) cells are central to retinal health and homoeostasis. Oxidative stress-induced damage to the RPE occurs as part of the pathogenesis of age-related macular degeneration and neovascular retinopathies (e.g., retinopathy of prematurity, diabetic retinopathy). The xanthophyll carotenoids, lutein and zeaxanthin, are selectively taken up by the RPE, preferentially accumulated in the human macula, and transferred to photoreceptors. These macular xanthophylls protect the macula (and the broader retina) via their antioxidant and photo-protective activities. This study was designed to investigate effects of various carotenoids (β-carotene, lycopene, and lutein) on RPE cells subjected to either hypoxia or oxidative stress, in order to determine if there is effect specificity for macular pigment carotenoids. Using human RPE-derived ARPE-19 cells as an in vitro model, we exposed RPE cells to various concentrations of the specific carotenoids, followed by either graded hypoxia or oxidative stress using tert-butyl hydroperoxide (tBHP). The results indicate that lutein and lycopene, but not β-carotene, inhibit cell growth in undifferentiated ARPE-19 cells. Moreover, cell viability was decreased under hypoxic conditions. Pre-incubation of ARPE-19 cells with lutein or lycopene protected against tBHP-induced cell loss and cell co-exposure of lutein or lycopene with tBHP essentially neutralized tBHP-dependent cell death at tBHP concentrations up to 500 μM. Our findings indicate that lutein and lycopene inhibit the growth of human RPE cells and protect the RPE against oxidative stress-induced cell loss. These findings contribute to the understanding of the protective mechanisms attributable to retinal xanthophylls in eye health and retinopathies. Full article

(This article belongs to the Special Issue Carotenoids—Antioxidant Properties)

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18 pages, 629 KiB

Open AccessReview

Recent Advances in our Understanding of Tocopherol Biosynthesis in Plants: An Overview of Key Genes, Functions, and Breeding of Vitamin E Improved Crops

by Steffi Fritsche^1,*, Xingxing Wang^1,2 and Christian Jung¹

¹ Plant Breeding Institute, Christian-Albrechts-University of Kiel, 24118 Kiel, Germany

² Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China

Antioxidants 2017, 6(4), 99; https://doi.org/10.3390/antiox6040099 - 1 Dec 2017

Cited by 147 | Viewed by 12505

Abstract

Tocopherols, together with tocotrienols and plastochromanols belong to a group of lipophilic compounds also called tocochromanols or vitamin E. Considered to be one of the most powerful antioxidants, tocochromanols are solely synthesized by photosynthetic organisms including plants, algae, and cyanobacteria and, therefore, are [...] Read more.

Tocopherols, together with tocotrienols and plastochromanols belong to a group of lipophilic compounds also called tocochromanols or vitamin E. Considered to be one of the most powerful antioxidants, tocochromanols are solely synthesized by photosynthetic organisms including plants, algae, and cyanobacteria and, therefore, are an essential component in the human diet. Tocochromanols potent antioxidative properties are due to their ability to interact with polyunsaturated acyl groups and scavenge lipid peroxyl radicals and quench reactive oxygen species (ROS), thus protecting fatty acids from lipid peroxidation. In the plant model species Arabidopsis thaliana, the required genes for tocopherol biosynthesis and functional roles of tocopherols were elucidated in mutant and transgenic plants. Recent research efforts have led to new outcomes for the vitamin E biosynthetic and related pathways, and new possible alternatives for the biofortification of important crops have been suggested. Here, we review 30 years of research on tocopherols in model and crop species, with emphasis on the improvement of vitamin E content using transgenic approaches and classical breeding. We will discuss future prospects to further improve the nutritional value of our food. Full article

(This article belongs to the Special Issue Vitamin E)

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10 pages, 1610 KiB

Open AccessArticle

Differential Effects of Superoxide Dismutase Mimetics after Mechanical Overload of Articular Cartilage

by Mitchell C. Coleman^1,*, Marc J. Brouillette¹, Nicholas S. Andresen¹, Rebecca E. Oberley-Deegan² and James M. Martin¹

¹ Department of Orthopedics and Rehabilitation, University of Iowa, Iowa City, IA 52242, USA

² Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA

Antioxidants 2017, 6(4), 98; https://doi.org/10.3390/antiox6040098 - 30 Nov 2017

Cited by 19 | Viewed by 4995

Abstract

Post-traumatic osteoarthritis can develop as a result of the initial mechanical impact causing the injury and also as a result of chronic changes in mechanical loading of the joint. Aberrant mechanical loading initiates excessive production of reactive oxygen species, oxidative damage, and stress [...] Read more.

Post-traumatic osteoarthritis can develop as a result of the initial mechanical impact causing the injury and also as a result of chronic changes in mechanical loading of the joint. Aberrant mechanical loading initiates excessive production of reactive oxygen species, oxidative damage, and stress that appears to damage mitochondria in the surviving chondrocytes. To probe the benefits of increasing superoxide removal with small molecular weight superoxide dismutase mimetics under severe loads, we applied both impact and overload injury scenarios to bovine osteochondral explants using characterized mechanical platforms with and without GC4403, MnTE-2-PyP, and MnTnBuOE-2-PyP. In impact scenarios, each of these mimetics provides some dose-dependent protection from cell death and loss of mitochondrial content while in repeated overloading scenarios only MnTnBuOE-2-PyP provided a clear benefit to chondrocytes. These results support the hypothesis that superoxide is generated in excess after impact injuries and suggest that superoxide production within the lipid compartment may be a critical mediator of responses to chronic overload. This is an important nuance distinguishing roles of superoxide, and thus superoxide dismutases, in mediating damage to cellular machinery in hyper-acute impact scenarios compared to chronic scenarios. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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14 pages, 700 KiB

Open AccessArticle

The Subcellular Distribution of Alpha-Tocopherol in the Adult Primate Brain and Its Relationship with Membrane Arachidonic Acid and Its Oxidation Products

by Emily S. Mohn^1,*, Matthew J. Kuchan², John W. Erdman³, Martha Neuringer⁴, Nirupa R. Matthan¹, Chung-Yen Oliver Chen¹ and Elizabeth J. Johnson¹

¹ Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA

² Discovery Research, Abbott Nutrition, Columbus, OH 43229, USA

³ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA

⁴ Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, USA

Antioxidants 2017, 6(4), 97; https://doi.org/10.3390/antiox6040097 - 26 Nov 2017

Cited by 13 | Viewed by 4285

Abstract

The relationship between α-tocopherol, a known antioxidant, and polyunsaturated fatty acid (PUFA) oxidation, has not been directly investigated in the primate brain. This study characterized the membrane distribution of α-tocopherol in brain regions and investigated the association between membrane α-tocopherol and PUFA content, [...] Read more.

The relationship between α-tocopherol, a known antioxidant, and polyunsaturated fatty acid (PUFA) oxidation, has not been directly investigated in the primate brain. This study characterized the membrane distribution of α-tocopherol in brain regions and investigated the association between membrane α-tocopherol and PUFA content, as well as brain PUFA oxidation products. Nuclear, myelin, mitochondrial, and neuronal membranes were isolated using a density gradient from the prefrontal cortex (PFC), cerebellum (CER), striatum (ST), and hippocampus (HC) of adult rhesus monkeys (n = 9), fed a stock diet containing vitamin E (α-, γ-tocopherol intake: ~0.7 µmol/kg body weight/day, ~5 µmol/kg body weight/day, respectively). α-tocopherol, PUFAs, and PUFA oxidation products were measured using high performance liquid chromatography (HPLC), gas chromatography (GC) and liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) respectively. α-Tocopherol (ng/mg protein) was highest in nuclear membranes (p < 0.05) for all regions except HC. In PFC and ST, arachidonic acid (AA, µg/mg protein) had a similar membrane distribution to α-tocopherol. Total α-tocopherol concentrations were inversely associated with AA oxidation products (isoprostanes) (p < 0.05), but not docosahexaenoic acid oxidation products (neuroprostanes). This study reports novel data on α-tocopherol accumulation in primate brain regions and membranes and provides evidence that α-tocopherol and AA are similarly distributed in PFC and ST membranes, which may reflect a protective effect of α-tocopherol against AA oxidation. Full article

(This article belongs to the Special Issue Vitamin E)

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33 pages, 4879 KiB

Open AccessReview

Carotenoids from Marine Organisms: Biological Functions and Industrial Applications

by Christian Galasso¹, Cinzia Corinaldesi^2,* and Clementina Sansone^1,*

¹ Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy

² Department of Sciences and Engineering of Materials, Environment and Urbanistics, Università Politecnica delle Marche, 60121 Ancona, Italy

Antioxidants 2017, 6(4), 96; https://doi.org/10.3390/antiox6040096 - 23 Nov 2017

Cited by 320 | Viewed by 14424

Abstract

As is the case for terrestrial organisms, carotenoids represent the most common group of pigments in marine environments. They are generally biosynthesized by all autotrophic marine organisms, such as bacteria and archaea, algae and fungi. Some heterotrophic organisms also contain carotenoids probably accumulated [...] Read more.

As is the case for terrestrial organisms, carotenoids represent the most common group of pigments in marine environments. They are generally biosynthesized by all autotrophic marine organisms, such as bacteria and archaea, algae and fungi. Some heterotrophic organisms also contain carotenoids probably accumulated from food or partly modified through metabolic reactions. These natural pigments are divided into two chemical classes: carotenes (such as lycopene and α- and β-carotene) that are composed of hydrogen and carbon; xanthophylls (such as astaxanthin, fucoxanthin and lutein), which are constituted by hydrogen, carbon and oxygen. Carotenoids, as antioxidant compounds, assume a key role in the protection of cells. In fact, quenching of singlet oxygen, light capture and photosynthesis protection are the most relevant biological functions of carotenoids. The present review aims at describing (i) the biological functions of carotenoids and their benefits for human health, (ii) the most common carotenoids from marine organisms and (iii) carotenoids having large success in pharmaceutical, nutraceutical and cosmeceutical industries, highlighting the scientific progress in marine species cultivation for natural pigments production. Full article

(This article belongs to the Special Issue Carotenoids—Antioxidant Properties)

11 pages, 382 KiB

Open AccessReview

Vitamin E Bioavailability: Mechanisms of Intestinal Absorption in the Spotlight

by Emmanuelle Reboul

Aix Marseille University, INRA, INSERM, C2VN, 13005 Marseille, France

Antioxidants 2017, 6(4), 95; https://doi.org/10.3390/antiox6040095 - 22 Nov 2017

Cited by 138 | Viewed by 14365

Abstract

Vitamin E is an essential fat-soluble micronutrient whose effects on human health can be attributed to both antioxidant and non-antioxidant properties. A growing number of studies aim to promote vitamin E bioavailability in foods. It is thus of major interest to gain deeper [...] Read more.

Vitamin E is an essential fat-soluble micronutrient whose effects on human health can be attributed to both antioxidant and non-antioxidant properties. A growing number of studies aim to promote vitamin E bioavailability in foods. It is thus of major interest to gain deeper insight into the mechanisms of vitamin E absorption, which remain only partly understood. It was long assumed that vitamin E was absorbed by passive diffusion, but recent data has shown that this process is actually far more complex than previously thought. This review describes the fate of vitamin E in the human gastrointestinal lumen during digestion and focuses on the proteins involved in the intestinal membrane and cellular transport of vitamin E across the enterocyte. Special attention is also given to the factors modulating both vitamin E micellarization and absorption. Although these latest results significantly improve our understanding of vitamin E intestinal absorption, further studies are still needed to decipher the molecular mechanisms driving this multifaceted process. Full article

(This article belongs to the Special Issue Vitamin E)

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9 pages, 176 KiB

Open AccessMeeting Report

Symposium on Vitamin C, 15th September 2017; Part of the Linus Pauling Institute’s 9th International Conference on Diet and Optimum Health

by Anitra C. Carr

Department of Pathology, University of Otago, Christchurch, PO Box 4345, Christchurch 8140, New Zealand

Antioxidants 2017, 6(4), 94; https://doi.org/10.3390/antiox6040094 - 21 Nov 2017

Cited by 1 | Viewed by 6270

Abstract

The Linus Pauling Institute’s 9th International Conference on Diet and Optimum Health took place on 13–15 September 2017 in Corvallis, OR, USA, on the beautiful Oregon State University campus [...]
Full article

(This article belongs to the Special Issue Vitamin C: Current Concepts in Human Physiology)

14 pages, 802 KiB

Open AccessReview

Tocotrienols: A Family of Molecules with Specific Biological Activities

by Raffaella Comitato, Roberto Ambra and Fabio Virgili^*

Council for Agricultural Research and Economics, Research Centre for Food and Nutrition (CREA-AN) via Ardeatina 546, 00178 Rome, Italy

Antioxidants 2017, 6(4), 93; https://doi.org/10.3390/antiox6040093 - 18 Nov 2017

Cited by 42 | Viewed by 6639

Abstract

Vitamin E is a generic term frequently used to group together eight different molecules, namely: α-, β-, γ- and δ-tocopherol and the corresponding tocotrienols. The term tocopherol and eventually Vitamin E and its related activity was originally based on the capacity of countering [...] Read more.

Vitamin E is a generic term frequently used to group together eight different molecules, namely: α-, β-, γ- and δ-tocopherol and the corresponding tocotrienols. The term tocopherol and eventually Vitamin E and its related activity was originally based on the capacity of countering foetal re-absorption in deficient rodents or the development of encephalomalacia in chickens. In humans, Vitamin E activity is generally considered to be solely related to the antioxidant properties of the tocolic chemical structure. In recent years, several reports have shown that specific activities exist for each different tocotrienol form. In this short review, tocotrienol ability to inhibit cancer cell growth and induce apoptosis thanks to specific mechanisms, not shared by tocopherols, such as the binding to Estrogen Receptor-β (ERβ) and the triggering of endoplasmic reticulum (EndoR) stress will be described. The neuroprotective activity will also be presented and discussed. We propose that available studies strongly indicate that specific forms of tocotrienols have a distinct mechanism and biological activity, significantly different from tocopherol and more specifically from α-tocopherol. We therefore suggest not pooling them together within the broad term “Vitamin E” on solely the basis of their putative antioxidant properties. This option implies obvious consequences in the assessment of dietary Vitamin E adequacy and, probably more importantly, on the possibility of evaluating a separate biological variable, determinant in the relationship between diet and health. Full article

(This article belongs to the Special Issue Vitamin E)

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15 pages, 3369 KiB

Open AccessArticle

MnSOD and Cyclin B1 Coordinate a Mito-Checkpoint during Cell Cycle Response to Oxidative Stress

by Amanda L. Kalen¹, Iman M. Ahmad², Maher Y. Abdalla³, Yunxia Q. O’Malley⁴, Prabhat C. Goswami¹ and Ehab H. Sarsour^1,*

¹ Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA 52242, USA

² Department of Medical Imaging and Therapeutic Sciences, College of Allied Health Professions, University of Nebraska Medical Center, Omaha, NE 68198, USA

³ Department of Pathology and Microbiology, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA

⁴ Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA

Antioxidants 2017, 6(4), 92; https://doi.org/10.3390/antiox6040092 - 17 Nov 2017

Cited by 7 | Viewed by 5701

Abstract

Communication between the nucleus and mitochondrion could coordinate many cellular processes. While the mechanisms regulating this communication are not completely understood, we hypothesize that cell cycle checkpoint proteins coordinate the cross-talk between nuclear and mitochondrial functions following oxidative stress. Human normal skin fibroblasts, [...] Read more.

Communication between the nucleus and mitochondrion could coordinate many cellular processes. While the mechanisms regulating this communication are not completely understood, we hypothesize that cell cycle checkpoint proteins coordinate the cross-talk between nuclear and mitochondrial functions following oxidative stress. Human normal skin fibroblasts, representative of the G₂-phase, were irradiated with 6 Gy of ionizing radiation and assayed for cyclin B1 translocation, mitochondrial function, reactive oxygen species (ROS) levels, and cytotoxicity. In un-irradiated controls, cyclin B1 was found primarily in the nucleus of G₂-cells. However, following irradiation, cyclin B1 was excluded from the nucleus and translocated to the cytoplasm and mitochondria. These observations were confirmed further by performing transmission electron microscopy and cell fractionation assays. Cyclin B1 was absent in mitochondria isolated from un-irradiated G₂-cells and present in irradiated G₂-cells. Radiation-induced translocation of cyclin B1 from the nucleus to the mitochondrion preceded changes in the activities of mitochondrial proteins, that included decreases in the activities of aconitase and the mitochondrial antioxidant enzyme, manganese superoxide dismutase (MnSOD), and increases in complex II activity. Changes in the activities of mito-proteins were followed by an increase in dihydroethidium (DHE) oxidation (indicative of increased superoxide levels) and loss of the mitochondrial membrane potential, events that preceded the restart of the stalled cell cycle and subsequently the loss in cell viability. Comparable results were also observed in un-irradiated control cells overexpressing mitochondria-targeted cyclin B1. These results indicate that MnSOD and cyclin B1 coordinate a cross-talk between nuclear and mitochondrial functions, to regulate a mito-checkpoint during the cell cycle response to oxidative stress. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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13 pages, 589 KiB

Open AccessReview

Bioactive Components in Moringa Oleifera Leaves Protect against Chronic Disease

by Marcela Vergara-Jimenez¹, Manal Mused Almatrafi² and Maria Luz Fernandez^2,*

¹ Department of Nutrition, Universidad Autonoma de Sinaloa, Culiacan 80019, Mexico

² Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA

Antioxidants 2017, 6(4), 91; https://doi.org/10.3390/antiox6040091 - 16 Nov 2017

Cited by 415 | Viewed by 39314

Abstract

Moringa Oleifera (MO), a plant from the family Moringacea is a major crop in Asia and Africa. MO has been studied for its health properties, attributed to the numerous bioactive components, including vitamins, phenolic acids, flavonoids, isothiocyanates, tannins and saponins, which [...] Read more.

Moringa Oleifera (MO), a plant from the family Moringacea is a major crop in Asia and Africa. MO has been studied for its health properties, attributed to the numerous bioactive components, including vitamins, phenolic acids, flavonoids, isothiocyanates, tannins and saponins, which are present in significant amounts in various components of the plant. Moringa Oleifera leaves are the most widely studied and they have shown to be beneficial in several chronic conditions, including hypercholesterolemia, high blood pressure, diabetes, insulin resistance, non-alcoholic liver disease, cancer and overall inflammation. In this review, we present information on the beneficial results that have been reported on the prevention and alleviation of these chronic conditions in various animal models and in cell studies. The existing limited information on human studies and Moringa Oleifera leaves is also presented. Overall, it has been well documented that Moringa Oleifera leaves are a good strategic for various conditions associated with heart disease, diabetes, cancer and fatty liver. Full article

(This article belongs to the Special Issue Dietary Antioxidants and Prevention of Non-Communicable Diseases)

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22 pages, 2483 KiB

Open AccessReview

Nox, Reactive Oxygen Species and Regulation of Vascular Cell Fate

by Denise Burtenshaw¹

, Roya Hakimjavadi¹, Eileen M. Redmond²

and Paul A. Cahill^1,*

¹ Vascular Biology & Therapeutics, School of Biotechnology, Dublin City University, D09NR58 Dublin, Ireland

² Department of Surgery (Research), University of Rochester Medical Centre, Rochester, NY 14620, USA

Antioxidants 2017, 6(4), 90; https://doi.org/10.3390/antiox6040090 - 14 Nov 2017

Cited by 104 | Viewed by 10368

Abstract

The generation of reactive oxygen species (ROS) and an imbalance of antioxidant defence mechanisms can result in oxidative stress. Several pro-atherogenic stimuli that promote intimal-medial thickening (IMT) and early arteriosclerotic disease progression share oxidative stress as a common regulatory pathway dictating vascular cell [...] Read more.

The generation of reactive oxygen species (ROS) and an imbalance of antioxidant defence mechanisms can result in oxidative stress. Several pro-atherogenic stimuli that promote intimal-medial thickening (IMT) and early arteriosclerotic disease progression share oxidative stress as a common regulatory pathway dictating vascular cell fate. The major source of ROS generated within the vascular system is the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase family of enzymes (Nox), of which seven members have been characterized. The Nox family are critical determinants of the redox state within the vessel wall that dictate, in part the pathophysiology of several vascular phenotypes. This review highlights the putative role of ROS in controlling vascular fate by promoting endothelial dysfunction, altering vascular smooth muscle phenotype and dictating resident vascular stem cell fate, all of which contribute to intimal medial thickening and vascular disease progression. Full article

(This article belongs to the Special Issue ROS Derived from NADPH Oxidase (NOX) in Angiogenesis)

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12 pages, 1407 KiB

Open AccessArticle

Trans-Plasma Membrane Electron Transport and Ascorbate Efflux by Skeletal Muscle

by Amanda M. Eccardt¹, Thomas P. Bell¹, Lyn Mattathil¹, Rohan Prasad², Shannon C. Kelly¹ and Jonathan S. Fisher^1,*

¹ Department of Biology, Saint Louis University, 3507 Laclede Ave, St. Louis, MO 63103, USA

² School of Medicine, Saint Louis University, 3507 Laclede Ave, St. Louis, MO 63103, USA

Antioxidants 2017, 6(4), 89; https://doi.org/10.3390/antiox6040089 - 9 Nov 2017

Cited by 12 | Viewed by 5976

Abstract

Trans-plasma membrane electron transport (tPMET) and the antioxidant roles of ascorbate reportedly play a role in protection of cells from damage by reactive oxygen species, which have been implicated in causing metabolic dysfunction such as insulin resistance. Skeletal muscle comprises the largest whole-body [...] Read more.

Trans-plasma membrane electron transport (tPMET) and the antioxidant roles of ascorbate reportedly play a role in protection of cells from damage by reactive oxygen species, which have been implicated in causing metabolic dysfunction such as insulin resistance. Skeletal muscle comprises the largest whole-body organ fraction suggesting a potential role of tPMET and ascorbate export as a major source of extracellular antioxidant. We hypothesized that skeletal muscle is capable of tPMET and ascorbate efflux. To measure these processes, we assayed the ability of cultured muscle cells, satellite cells, and isolated extensor digitorum longus (EDL) and soleus (SOL) to reduce two extracellular electron acceptors, water soluble tetrazolium salt 1 (WST-1), and dichlorophenolindophenol (DPIP). Ascorbate oxidase (AO) was utilized to determine which portion of WST-1 reduction was dependent on ascorbate efflux. We found that muscle cells can reduce extracellular electron acceptors. In C2C12 myotubes and satellite cells, a substantial portion of this reduction was dependent on ascorbate. In myotubes, glucose transporter 1 (GLUT1) inhibitors along with a pan-GLUT inhibitor suppressed tPMET and ascorbate efflux, while a GLUT4 inhibitor had no effect. The adenosine 5′-monophosphate (AMP)-activated protein kinase activator 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR) suppressed both tPMET and ascorbate efflux by myotubes, while insulin had no effect. Taken together, our data suggest that muscle cells are capable of tPMET and ascorbate efflux supported by GLUT1, thus illustrating a model in which resting muscle exports electrons and antioxidant to the extracellular environment. Full article

(This article belongs to the Special Issue Exercise Induced Muscle Damage and Oxidative Stress)

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24 pages, 1820 KiB

Open AccessReview

Polyphenols as Promising Drugs against Main Breast Cancer Signatures

by María Losada-Echeberría¹

, María Herranz-López¹

, Vicente Micol^1,2 and Enrique Barrajón-Catalán^1,*

¹ Institute of Molecular and Cell Biology (IBMC), Miguel Hernández University (UMH), Avda. Universidad s/n, Elche 03202, Spain

² CIBER, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III (CB12/03/30038), Palma de Mallorca 07122, Spain

Antioxidants 2017, 6(4), 88; https://doi.org/10.3390/antiox6040088 - 7 Nov 2017

Cited by 69 | Viewed by 11297

Abstract

Breast cancer is one of the most common neoplasms worldwide, and in spite of clinical and pharmacological advances, it is still a clinical problem, causing morbidity and mortality. On the one hand, breast cancer shares with other neoplasms some molecular signatures such as [...] Read more.

Breast cancer is one of the most common neoplasms worldwide, and in spite of clinical and pharmacological advances, it is still a clinical problem, causing morbidity and mortality. On the one hand, breast cancer shares with other neoplasms some molecular signatures such as an imbalanced redox state, cell cycle alterations, increased proliferation and an inflammatory status. On the other hand, breast cancer shows differential molecular subtypes that determine its prognosis and treatment. These are characterized mainly by hormone receptors especially estrogen receptors (ERs) and epidermal growth factor receptor 2 (HER2). Tumors with none of these receptors are classified as triple negative breast cancer (TNBC) and are associated with a worse prognosis. The success of treatments partially depends on their specificity and the adequate molecular classification of tumors. New advances in anticancer drug discovery using natural compounds have been made in the last few decades, and polyphenols have emerged as promising molecules. They may act on various molecular targets because of their promiscuous behavior, presenting several physiological effects, some of which confer antitumor activity. This review analyzes the accumulated evidence of the antitumor effects of plant polyphenols on breast cancer, with special attention to their activity on ERs and HER2 targets and also covering different aspects such as redox balance, uncontrolled proliferation and chronic inflammation. Full article

(This article belongs to the Special Issue Dietary Antioxidants and Prevention of Non-Communicable Diseases)

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22 pages, 13465 KiB

Open AccessArticle

The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues

by Shashank Shrishrimal¹, Elizabeth A. Kosmacek¹, Arpita Chatterjee¹, McDonald J. Tyson²

and Rebecca E. Oberley-Deegan^1,*

¹ Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA

² Department of Physics & Cancer Research Center, Hampton University, Hampton, VA 23668, USA

Antioxidants 2017, 6(4), 87; https://doi.org/10.3390/antiox6040087 - 6 Nov 2017

Cited by 29 | Viewed by 6741

Abstract

Pelvic radiation for cancer therapy can damage a variety of normal tissues. In this study, we demonstrate that radiation causes acute changes to pelvic fibroblasts such as the transformation to myofibroblasts and the induction of senescence, which persist months after radiation. The addition [...] Read more.

Pelvic radiation for cancer therapy can damage a variety of normal tissues. In this study, we demonstrate that radiation causes acute changes to pelvic fibroblasts such as the transformation to myofibroblasts and the induction of senescence, which persist months after radiation. The addition of the manganese porphyrin, MnTE-2-PyP, resulted in protection of these acute changes in fibroblasts and this protection persisted months following radiation exposure. Specifically, at two months post-radiation, MnTE-2-PyP inhibited the number of α-smooth muscle actin positive fibroblasts induced by radiation and at six months post-radiation, MnTE-2-PyP significantly reduced collagen deposition (fibrosis) in the skin and bladder tissues of irradiated mice. Radiation also resulted in changes to T cells. At two months post-radiation, there was a reduction of Th1-producing splenocytes, which resulted in reduced Th1:Th2 ratios. MnTE-2-PyP maintained Th1:Th2 ratios similar to unirradiated mice. At six months post-radiation, increased T cells were observed in the adipose tissues. MnTE-2-PyP treatment inhibited this increase. Thus, MnTE-2-PyP treatment maintains normal fibroblast function and T cell immunity months after radiation exposure. We believe that one of the reasons MnTE-2-PyP is a potent radioprotector is due to its protection of multiple cell types from radiation damage. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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25 pages, 1517 KiB

Open AccessReview

Insights into the Dichotomous Regulation of SOD2 in Cancer

by Yeon Soo Kim^1,†, Piyushi Gupta Vallur^1,†, Rébécca Phaëton², Karthikeyan Mythreye³ and Nadine Hempel^1,*

¹ Department of Pharmacology, College of Medicine, Penn State University, Hershey, PA 17033, USA

² Department of Obstetrics & Gynecology & Department of Microbiology and Immunology, College of Medicine, Penn State University, Hershey, PA 17033, USA

³ Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA

^† These authors contributed equally to this work.

Antioxidants 2017, 6(4), 86; https://doi.org/10.3390/antiox6040086 - 3 Nov 2017

Cited by 136 | Viewed by 16765

Abstract

While loss of antioxidant expression and the resultant oxidant-dependent damage to cellular macromolecules is key to tumorigenesis, it has become evident that effective oxidant scavenging is conversely necessary for successful metastatic spread. This dichotomous role of antioxidant enzymes in cancer highlights their context-dependent [...] Read more.

While loss of antioxidant expression and the resultant oxidant-dependent damage to cellular macromolecules is key to tumorigenesis, it has become evident that effective oxidant scavenging is conversely necessary for successful metastatic spread. This dichotomous role of antioxidant enzymes in cancer highlights their context-dependent regulation during different stages of tumor development. A prominent example of an antioxidant enzyme with such a dichotomous role and regulation is the mitochondria-localized manganese superoxide dismutase SOD2 (MnSOD). SOD2 has both tumor suppressive and promoting functions, which are primarily related to its role as a mitochondrial superoxide scavenger and H₂O₂ regulator. However, unlike true tumor suppressor- or onco-genes, the SOD2 gene is not frequently lost, or rarely mutated or amplified in cancer. This allows SOD2 to be either repressed or activated contingent on context-dependent stimuli, leading to its dichotomous function in cancer. Here, we describe some of the mechanisms that underlie SOD2 regulation in tumor cells. While much is known about the transcriptional regulation of the SOD2 gene, including downregulation by epigenetics and activation by stress response transcription factors, further research is required to understand the post-translational modifications that regulate SOD2 activity in cancer cells. Moreover, future work examining the spatio-temporal nature of SOD2 regulation in the context of changing tumor microenvironments is necessary to allows us to better design oxidant- or antioxidant-based therapeutic strategies that target the adaptable antioxidant repertoire of tumor cells. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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18 pages, 4704 KiB

Open AccessArticle

Treatment with a Catalytic Superoxide Dismutase (SOD) Mimetic Improves Liver Steatosis, Insulin Sensitivity, and Inflammation in Obesity-Induced Type 2 Diabetes

by Gina M. Coudriet^1,†

, Meghan M. Delmastro-Greenwood^2,†, Dana M. Previte¹

, Meghan L. Marré¹, Erin C. O’Connor¹, Elizabeth A. Novak¹, Garret Vincent¹, Kevin P. Mollen¹, Sojin Lee², H. Henry Dong² and Jon D. Piganelli^1,*

¹ Department of Surgery, Children’s Hospital of Pittsburgh of UPMC, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15224, USA

² Department of Pediatrics, Children’s Hospital of Pittsburgh of UPMC, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15224, USA

^† These authors contributed equally to this work.

Antioxidants 2017, 6(4), 85; https://doi.org/10.3390/antiox6040085 - 1 Nov 2017

Cited by 44 | Viewed by 8976

Abstract

Oxidative stress and persistent inflammation are exaggerated through chronic over-nutrition and a sedentary lifestyle, resulting in insulin resistance. In type 2 diabetes (T2D), impaired insulin signaling leads to hyperglycemia and long-term complications, including metabolic liver dysfunction, resulting in non-alcoholic fatty liver disease (NAFLD). [...] Read more.

Oxidative stress and persistent inflammation are exaggerated through chronic over-nutrition and a sedentary lifestyle, resulting in insulin resistance. In type 2 diabetes (T2D), impaired insulin signaling leads to hyperglycemia and long-term complications, including metabolic liver dysfunction, resulting in non-alcoholic fatty liver disease (NAFLD). The manganese metalloporphyrin superoxide dismustase (SOD) mimetic, manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl) porphyrin (MnP), is an oxidoreductase known to scavenge reactive oxygen species (ROS) and decrease pro-inflammatory cytokine production, by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation. We hypothesized that targeting oxidative stress-induced inflammation with MnP would assuage liver complications and enhance insulin sensitivity and glucose tolerance in a high-fat diet (HFD)-induced mouse model of T2D. During 12 weeks of feeding, we saw significant improvements in weight, hepatic steatosis, and biomarkers of liver dysfunction with redox modulation by MnP treatment in HFD-fed mice. Additionally, MnP treatment improved insulin sensitivity and glucose tolerance, while reducing serum insulin and leptin levels. We attribute these effects to redox modulation and inhibition of hepatic NF-κB activation, resulting in diminished ROS and pro-inflammatory cytokine production. This study highlights the importance of controlling oxidative stress and secondary inflammation in obesity-mediated insulin resistance and T2D. Our data confirm the role of NF-κB-mediated inflammation in the development of T2D, and demonstrate the efficacy of MnP in preventing the progression to disease by specifically improving liver pathology and hepatic insulin resistance in obesity. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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16 pages, 274 KiB

Open AccessReview

Effects of Cocoa Antioxidants in Type 2 Diabetes Mellitus

by Sonia Ramos¹

, María Angeles Martín^1,2 and Luis Goya^1,*

¹ Department of Metabolism and Nutrition. Institute of Food Science and Technology and Nutrition (ICTAN-CSIC). José Antonio Novais 10. Ciudad Universitaria, 28040 Madrid, Spain

² Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Instituto de Salud Carlos III (ISCIII), 28040 Madrid, Spain

Antioxidants 2017, 6(4), 84; https://doi.org/10.3390/antiox6040084 - 31 Oct 2017

Cited by 55 | Viewed by 16261

Abstract

Type 2 Diabetes mellitus (T2D) is the most common form of diabetes and one of the most common chronic diseases. Control of hyperglycaemia by hypoglycaemic drugs is insufficient in for patients and nutritional approaches are currently being explored. Natural dietary compounds such as [...] Read more.

Type 2 Diabetes mellitus (T2D) is the most common form of diabetes and one of the most common chronic diseases. Control of hyperglycaemia by hypoglycaemic drugs is insufficient in for patients and nutritional approaches are currently being explored. Natural dietary compounds such as flavonoids, abundant in fruits and vegetables, have received broad attention because of their potential capacity to act as anti-diabetic agents. Especially cocoa flavonoids have been proved to ameliorate important hallmarks of T2D. In this review, an update of the most relevant reports published during the last decade in cell culture, animal models and human studies is presented. Most results support an anti-diabetic effect of cocoa flavonoids by enhancing insulin secretion, improving insulin sensitivity in peripheral tissues, exerting a lipid-lowering effect and preventing the oxidative and inflammatory damages associated to the disease. While it could be suggested that daily consumption of flavanols from cocoa or dark chocolate would constitute a potential preventive tool useful for the nutritional management of T2D, this recommendation should be cautious since most of commercially available soluble cocoa products or chocolates contain low amount of flavanols and are rich in sugar and calories that may aggravate glycaemic control in T2D patients. Full article

(This article belongs to the Special Issue Dietary Antioxidants and Prevention of Non-Communicable Diseases)

15 pages, 881 KiB

Open AccessArticle

Selenium Concentrations for Maximisation of Thioredoxin Reductase 2 Activity and Upregulation of Its Gene Transcripts in Senescent Human Fibroblasts

by Hazem K. Ghneim

Chair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia

Antioxidants 2017, 6(4), 83; https://doi.org/10.3390/antiox6040083 - 30 Oct 2017

Cited by 7 | Viewed by 4465

Abstract

Thioredoxin reductase 2 (TR2) activity, its gene transcripts, and hydrogen peroxide (H₂O₂) generation were examined in biochemically identified early-senescent P20 and senescent P30 fibroblasts subcultured in media (MEM2–MEM8) containing Se concentrations at 1.25, 2.5, 3.5, 5.0, 6.0, 7.0, and [...] Read more.

Thioredoxin reductase 2 (TR2) activity, its gene transcripts, and hydrogen peroxide (H₂O₂) generation were examined in biochemically identified early-senescent P20 and senescent P30 fibroblasts subcultured in media (MEM2–MEM8) containing Se concentrations at 1.25, 2.5, 3.5, 5.0, 6.0, 7.0, and 8.0 µM, respectively. Although TR2 activity was moderately increased in P20 and P30 cells subcultured in routine growth medium (MEM1), there were progressive significant activity increases in the same cells subcultured in MEM2–MEM8. Such increases were proportional to Se concentration and peaked in P30 cells incubated with MEM7 and MEM8. H₂O₂generation underwent progressiveincreases in MEM1-incubated P20 and P30 cells, peaking in the latter, but was gradually lowered in those incubated with MEM2–MEM8, reaching its lowest values when cells were incubated with MEM7 and MEM8. In parallel, TR2 gene transcripts underwent significant upregulation in P20 cells and higher magnitude upregulation in P30 cells subcultured in MEM2, MEM4, and MEM8 compared to those recorded for P5 pre-senescent cells subcultured in the same media. The computed Km Se values with respect to TR2 activity equaled 3.34 and 4.98 µM for P20 and P30 cells, respectively, with corresponding V_max activities of 55.9 and 96.2 nmol/min/mg protein. It is concluded that senescent P30 cells utilize more Se and achieve maximal TR2 activity to combat oxidative injury. Full article

(This article belongs to the Special Issue Selenium and Selenoproteins for Optimal Health)

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21 pages, 284 KiB

Open AccessReview

On the Origin of Superoxide Dismutase: An Evolutionary Perspective of Superoxide-Mediated Redox Signaling

by Adam J. Case

Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198, USA

Antioxidants 2017, 6(4), 82; https://doi.org/10.3390/antiox6040082 - 30 Oct 2017

Cited by 123 | Viewed by 11059

Abstract

The field of free radical biology originated with the discovery of superoxide dismutase (SOD) in 1969. Over the last 5 decades, a plethora of research has been performed in species ranging from bacteria to mammals that has elucidated the molecular reaction, subcellular location, [...] Read more.

The field of free radical biology originated with the discovery of superoxide dismutase (SOD) in 1969. Over the last 5 decades, a plethora of research has been performed in species ranging from bacteria to mammals that has elucidated the molecular reaction, subcellular location, and specific isoforms of SOD. However, while humans have only begun to study this class of enzymes over the past 50 years, it has been estimated that these enzymes have existed for billions of years, and may be some of the original enzymes found in primitive life. As life evolved over this expanse of time, these enzymes have taken on new and different functional roles potentially in contrast to how they were originally derived. Herein, examination of the evolutionary history of these enzymes provides both an explanation and further inquiries into the modern-day role of SOD in physiology and disease. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

20 pages, 308 KiB

Open AccessReview

The Role of Food Antioxidants, Benefits of Functional Foods, and Influence of Feeding Habits on the Health of the Older Person: An Overview

by Douglas W. Wilson^1,2,*, Paul Nash², Harpal Singh Buttar³, Keith Griffiths⁴, Ram Singh⁵, Fabien De Meester⁶, Rie Horiuchi⁷ and Toru Takahashi⁸

¹ School Medicine Pharmacy and Health, Durham University, Durham TS17 6BH, UK

² Centre for Ageing & Dementia Research, Swansea University, Swansea SA2 8PP, UK

³ Department of Pathology & Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada

⁴ Tenovus Institute for Cancer Research, Cardiff University, Laurel Cottage, Castleton, Gwent CF3 2UR, UK

⁵ Halberg Hospital and Research Institute, Civil Lines, Moradabad, UP 244001, India

⁶ TsimTsoum Institute, Ulica Gołębia 2, Kraków 31-007, Poland

⁷ Department of Food Sciences and Nutrition, Faculty of Human Environmental Sciences, Mukogawa Women’s University, 6-46 Ikebiraki-cho, Nishinomiya 663-8558, Japan

⁸ Graduate School of Human Environment Science, Fukuoka Women’s University, 1-1-1 Kasumigaoka, Higashi-ku, Fukuoka 813-8529, Japan

Antioxidants 2017, 6(4), 81; https://doi.org/10.3390/antiox6040081 - 28 Oct 2017

Cited by 179 | Viewed by 21380

Abstract

This overview was directed towards understanding the relationship of brain functions with dietary choices mainly by older humans. This included food color, flavor, and aroma, as they relate to dietary sufficiency or the association of antioxidants with neurodegenerative diseases such as dementia and [...] Read more.

This overview was directed towards understanding the relationship of brain functions with dietary choices mainly by older humans. This included food color, flavor, and aroma, as they relate to dietary sufficiency or the association of antioxidants with neurodegenerative diseases such as dementia and Alzheimer’s disease. Impairment of olfactory and gustatory function in relation to these diseases was also explored. The role of functional foods was considered as a potential treatment of dementia and Alzheimer’s disease through inhibition of acetylcholinesterase as well as similar treatments based on herbs, spices and antioxidants therein. The importance of antioxidants for maintaining the physiological functions of liver, kidney, digestive system, and prevention of cardiovascular diseases and cancer has also been highlighted. Detailed discussion was focused on health promotion of the older person through the frequency and patterns of dietary intake, and a human ecology framework to estimate adverse risk factors for health. Finally, the role of the food industry, mass media, and apps were explored for today’s new older person generation. Full article

(This article belongs to the Special Issue Dietary Antioxidants and Health Promotion)

17 pages, 1808 KiB

Open AccessReview

Photo Protection of Haematococcus pluvialis Algae by Astaxanthin: Unique Properties of Astaxanthin Deduced by EPR, Optical and Electrochemical Studies

by A. Ligia Focsan^1,*, Nikolay E. Polyakov² and Lowell D. Kispert³

¹ Department of Chemistry, Valdosta State University, Valdosta, GA 31698, USA

² Institute of Chemical Kinetics and Combustion, Novosibirsk 630090, Russia

³ Department of Chemistry, University of Alabama, Tuscaloosa, AL 35487, USA

Antioxidants 2017, 6(4), 80; https://doi.org/10.3390/antiox6040080 - 21 Oct 2017

Cited by 38 | Viewed by 9759

Abstract

Abstract The antioxidant astaxanthin is known to accumulate in Haematococcus pluvialis algae under unfavorable environmental conditions for normal cell growth. The accumulated astaxanthin functions as a protective agent against oxidative stress damage, and tolerance to excessive reactive oxygen species (ROS) is greater in [...] Read more.

Abstract The antioxidant astaxanthin is known to accumulate in Haematococcus pluvialis algae under unfavorable environmental conditions for normal cell growth. The accumulated astaxanthin functions as a protective agent against oxidative stress damage, and tolerance to excessive reactive oxygen species (ROS) is greater in astaxanthin-rich cells. The detailed mechanisms of protection have remained elusive, however, our Electron Paramagnetic Resonance (EPR), optical and electrochemical studies on carotenoids suggest that astaxanthin’s efficiency as a protective agent could be related to its ability to form chelate complexes with metals and to be esterified, its inability to aggregate in the ester form, its high oxidation potential and the ability to form proton loss neutral radicals under high illumination in the presence of metal ions. The neutral radical species formed by deprotonation of the radical cations can be very effective quenchers of the excited states of chlorophyll under high irradiation. Full article

(This article belongs to the Special Issue Carotenoids—Antioxidant Properties)

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9 pages, 388 KiB

Open AccessArticle

The Effect of Taurine on the Recovery from Eccentric Exercise-Induced Muscle Damage in Males

by Yanita McLeay^*

, Stephen Stannard and Matthew Barnes

School of Sport, Exercise, and Nutrition, Massey University, Palmerston North 4474, New Zealand

Antioxidants 2017, 6(4), 79; https://doi.org/10.3390/antiox6040079 - 17 Oct 2017

Cited by 26 | Viewed by 10915

Abstract

Eccentric exercise is known to bring about microstructural damage to muscle, initiating an inflammatory cascade involving various reactive oxygen species. This, in turn, can significantly impair physical performance over subsequent days. Taurine, a powerful endogenous antioxidant, has previously been shown to have a [...] Read more.

Eccentric exercise is known to bring about microstructural damage to muscle, initiating an inflammatory cascade involving various reactive oxygen species. This, in turn, can significantly impair physical performance over subsequent days. Taurine, a powerful endogenous antioxidant, has previously been shown to have a beneficial effect on muscle damage markers and recovery when taken for a few days to several weeks prior to eccentric exercise. However, to date no studies have looked at the effects of supplementing over the days following eccentric exercise on performance recovery. Thus, this study aimed to determine whether supplementing with taurine over three days following eccentric exercise attenuated the rise in serum creatine kinase and improved performance recovery in males. In a blinded, randomized, crossover design, ten recreationally-fit male participants completed 60 eccentric contractions of the biceps brachii muscle at maximal effort. Following this, participants were supplemented with 0.1 g∙kg⁻¹ body weight∙day⁻¹ of either taurine or rice flour in capsules. Over the next three mornings participants underwent blood tests for the analysis of the muscle damage marker creatine kinase and carried out performance measures on the isokinetic dynamometer. They also continued to consume the capsules in the morning and evening. The entire protocol was repeated two weeks later on the alternate arm and supplement. Significant decreases were seen in all performance measures from pre- to 24-h post-eccentric exercise (p < 0.001) for both taurine and placebo, indicating the attainment of muscle damage. Significant treatment effects were observed only for peak eccentric torque (p < 0.05). No significant time × treatment effects were observed (all p > 0.05). Serum creatine kinase levels did not significantly differ over time for either treatments, nor between treatments (p > 0.05). These findings suggest that taurine supplementation taken twice daily for 72 h following eccentric exercise-induced muscle damage may help improve eccentric performance recovery of the biceps brachii. Full article

(This article belongs to the Special Issue Exercise Induced Muscle Damage and Oxidative Stress)

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11 pages, 1103 KiB

Open AccessArticle

Total Phenolic and Yellow Pigment Contents and Antioxidant Activities of Durum Wheat Milling Fractions

by Bin Xiao Fu^1,*, Constance Chiremba¹, Curtis J. Pozniak², Kun Wang¹ and Shin Nam¹

¹ Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

² Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada

Antioxidants 2017, 6(4), 78; https://doi.org/10.3390/antiox6040078 - 14 Oct 2017

Cited by 23 | Viewed by 6053

Abstract

The aim of this study was to investigate the distribution of total yellow pigments, total phenolic compounds, and their antioxidant activities in various durum wheat milling fractions. Carotenoid composition of yellow pigment extract was also examined using UPLC. The ABTS radical scavenging activity [...] Read more.

The aim of this study was to investigate the distribution of total yellow pigments, total phenolic compounds, and their antioxidant activities in various durum wheat milling fractions. Carotenoid composition of yellow pigment extract was also examined using UPLC. The ABTS radical scavenging activity of the milling fractions decreased in the order of short bran/bran > feed flour > flour/semolina in both total phenolic and total yellow pigment extracts. Yellow pigments extracts from bran, short bran, and feed flour exhibited 5.6–15.4% higher antioxidant activity than those of total phenolic extracts from the corresponding milling fractions. The UPLC results showed a non-carotenoid peak at Rt 0.47 min which was present in fractions of the grain outer layers but absent in semolina and flour. This peak absorbed in the UV range of 271 to 327 nm. These observations suggest that the unknown peak could be composed of phenolic compounds co-extracted in their free form with carotenoids in the polar water-saturated butanol solvent. The compounds in this peak could result in overestimation of carotenoid content and antioxidant activity in bran, short bran and feed flour as the peak contributed to 18.3–26.0% of total carotenoids if it was taken into account. Full article

(This article belongs to the Special Issue Carotenoids—Antioxidant Properties)

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19 pages, 8379 KiB

Open AccessArticle

Procyanidin B2 Protects Neurons from Oxidative, Nitrosative, and Excitotoxic Stress

by Taylor C. Sutcliffe¹, Aimee N. Winter¹, Noelle C. Punessen¹ and Daniel A. Linseman^1,2,*

¹ Department of Biological Sciences, University of Denver, Denver, CO 80208, USA

² Knoebel Institute for Healthy Aging, University of Denver, Denver, CO 80208, USA

Antioxidants 2017, 6(4), 77; https://doi.org/10.3390/antiox6040077 - 13 Oct 2017

Cited by 48 | Viewed by 6369

Abstract

The aberrant generation of oxygen and nitrogen free radicals can cause severe damage to key cellular components, resulting in cell apoptosis. Similarly, excitotoxicity leads to protease activation and mitochondrial dysfunction, which subsequently causes cell death. Each of these factors play critical roles in [...] Read more.

The aberrant generation of oxygen and nitrogen free radicals can cause severe damage to key cellular components, resulting in cell apoptosis. Similarly, excitotoxicity leads to protease activation and mitochondrial dysfunction, which subsequently causes cell death. Each of these factors play critical roles in the neuronal cell death underlying various neurodegenerative diseases. Procyanidin B2 (PB2) is a naturally occurring polyphenolic compound found in high concentrations in cocoa, apples, and grapes. Here, we examine the neuroprotective effects of PB2 in primary cultures of rat cerebellar granule neurons (CGNs) exposed to various stressors. CGNs were pre-incubated with PB2 and then neuronal stress was induced as described below. Mitochondrial oxidative stress was triggered with HA14-1, an inhibitor of the pro-survival Bcl-2 protein which induces glutathione-sensitive apoptosis. Glutamate and glycine were used to induce excitotoxicity. Sodium nitroprusside, a nitric oxide generating compound, was used to induce nitrosative stress. We observed significant dose-dependent protection of CGNs with PB2 for all of the above insults, with the greatest neuroprotective effect being observed under conditions of nitrosative stress. Intriguingly, the neuroprotective effect of PB2 against nitric oxide was superoxide-dependent, as we have recently shown for other catechol antioxidants. Finally, we induced neuronal stress through the removal of depolarizing extracellular potassium and serum (5K conditions), which is a classical model of intrinsic apoptosis in CGNs. PB2 did not display any significant protection against 5K-induced apoptosis at any concentration tested. We conclude that PB2 offers neuronal protection principally as an antioxidant by scavenging reactive oxygen and nitrogen species instead of through modulation of pro-survival cell signaling pathways. These findings suggest that PB2 may be an effective neuroprotective agent for the treatment of neurodegenerative disorders. Full article

(This article belongs to the Special Issue Antioxidant and Neuroprotection)

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17 pages, 1841 KiB

Open AccessArticle

S-Adenosylmethionine and Superoxide Dismutase 1 Synergistically Counteract Alzheimer’s Disease Features Progression in TgCRND8 Mice

by Rosaria A. Cavallaro¹, Vincenzina Nicolia¹, Maria Teresa Fiorenza^2,3, Sigfrido Scarpa¹ and Andrea Fuso^1,2,*

¹ Department of Surgery “P. Valdoni”, Sapienza University of Rome, Via A. Scarpa 14, 00161 Rome, Italy

² Division of Neuroscience, Department of Psychology, Sapienza University of Rome, Via dei Marsi 78, 00183 Rome, Italy

³ IRCCS Santa Lucia Foundation, Via del Fosso di Fiorano 64-65, 00143 Rome, Italy

Antioxidants 2017, 6(4), 76; https://doi.org/10.3390/antiox6040076 - 30 Sep 2017

Cited by 20 | Viewed by 8762

Abstract

Recent evidence emphasizes the role of dysregulated one-carbon metabolism in Alzheimer’s Disease (AD). Exploiting a nutritional B-vitamin deficiency paradigm, we have previously shown that PSEN1 and BACE1 activity is modulated by one-carbon metabolism, leading to increased amyloid production. We have also demonstrated that [...] Read more.

Recent evidence emphasizes the role of dysregulated one-carbon metabolism in Alzheimer’s Disease (AD). Exploiting a nutritional B-vitamin deficiency paradigm, we have previously shown that PSEN1 and BACE1 activity is modulated by one-carbon metabolism, leading to increased amyloid production. We have also demonstrated that S-adenosylmethionine (SAM) supplementation contrasted the AD-like features, induced by B-vitamin deficiency. In the present study, we expanded these observations by investigating the effects of SAM and SOD (Superoxide dismutase) association. TgCRND8 AD mice were fed either with a control or B-vitamin deficient diet, with or without oral supplementation of SAM + SOD. We measured oxidative stress by lipid peroxidation assay, PSEN1 and BACE1 expression by Real-Time Polymerase Chain Reaction (PCR), amyloid deposition by ELISA assays and immunohistochemistry. We found that SAM + SOD supplementation prevents the exacerbation of AD-like features induced by B vitamin deficiency, showing synergistic effects compared to either SAM or SOD alone. SAM + SOD supplementation also contrasts the amyloid deposition typically observed in TgCRND8 mice. Although the mechanisms underlying the beneficial effect of exogenous SOD remain to be elucidated, our findings identify that the combination of SAM + SOD could be carefully considered as co-adjuvant of current AD therapies. Full article

(This article belongs to the Special Issue Antioxidant and Neuroprotection)

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Open AccessReview

Chemotherapy-Induced Tissue Injury: An Insight into the Role of Extracellular Vesicles-Mediated Oxidative Stress Responses

by Chontida Yarana^1,2 and Daret K. St. Clair^1,*

¹ Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY 40536, USA

² Faculty of Medical Technology, Mahidol University, Salaya 73170, Thailand

Antioxidants 2017, 6(4), 75; https://doi.org/10.3390/antiox6040075 - 28 Sep 2017

Cited by 62 | Viewed by 9232

Abstract

The short- and long-term side effects of chemotherapy limit the maximum therapeutic dose and impair quality of life of survivors. Injury to normal tissues, especially chemotherapy-induced cardiomyopathy, is an unintended outcome that presents devastating health impacts. Approximately half of the drugs approved by [...] Read more.

The short- and long-term side effects of chemotherapy limit the maximum therapeutic dose and impair quality of life of survivors. Injury to normal tissues, especially chemotherapy-induced cardiomyopathy, is an unintended outcome that presents devastating health impacts. Approximately half of the drugs approved by the Food and Drug Administration for cancer treatment are associated with the generation of reactive oxygen species, and Doxorubicin (Dox) is one of them. Dox undergoes redox cycling by involving its quinone structure in the production of superoxide free radicals, which are thought to be instrumental to the role it plays in cardiomyopathy. Dox-induced protein oxidation changes protein function, translocation, and aggregation that are toxic to cells. To maintain cellular homeostasis, oxidized proteins can be degraded intracellularly by ubiquitin-proteasome pathway or by autophagy, depending on the redox status of the cell. Alternatively, the cell can remove oxidized proteins by releasing extracellular vesicles (EVs), which can be transferred to neighboring or distant cells, thereby instigating an intercellular oxidative stress response. In this article, we discuss the role of EVs in oxidative stress response, the potential of EVs as sensitive biomarkers of oxidative stress, and the role of superoxide dismutase in attenuating EV-associated oxidative stress response resulting from chemotherapy. Full article

(This article belongs to the Special Issue Superoxide Dismutase (SOD) Enzymes, Mimetics and Oxygen Radicals)

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Antioxidants, Volume 6, Issue 4 (December 2017) – 32 articles

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