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Agronomy, Volume 9, Issue 4 (April 2019)
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Cover Story (view full-size image) Magnaporthiopsis maydis is considered the most serious maize fungal pathogen in Israel and Egypt, [...] Read more. Magnaporthiopsis maydis is considered the most serious maize fungal pathogen in Israel and Egypt, and a major threat in other countries. The pathogen causes late wilt, a destructive disease outbreak at the advanced growth stage, leading to severe dehydration of maize susceptible cultivars. Here, we review the methods developed in the past decade in Israel. The assays inspected are exemplified with new findings and include microbial isolation, microscopic and molecular identification, spore germination evaluation, detached roots, seeds, sprouts, and field pathogenicity assays. These diagnostic protocols enable rapid and reliable detection and identification of M. maydis in plants and seeds, studying its pathogenesis and population structure, evaluating cultivar resistance and preventive treatments, and developing new strategies to restrict the disease’s outburst and spread. View this paper