Nutrients

17 pages, 293 KB

Open AccessArticle

Differences in Neurocognitive Development Between Children Who Had Had No Breast Milk and Those Who Had Had Breast Milk for at Least 6 Months

by Neil Goulding, Kate Northstone, Caroline M. Taylor, Pauline Emmett, Yasmin Iles-Caven, Jacqueline Gregory, Steven Gregory and Jean Golding

Nutrients 2025, 17(17), 2847; https://doi.org/10.3390/nu17172847 (registering DOI) - 31 Aug 2025

Abstract

Background: There is considerable evidence that breast feeding has a beneficial effect on the neurocognition of a child. However, most studies have confined their attention to the Intelligence Quotient (IQ), tending to ignore other aspects of neurodevelopment. Methodology: Here we present [...] Read more.

Background: There is considerable evidence that breast feeding has a beneficial effect on the neurocognition of a child. However, most studies have confined their attention to the Intelligence Quotient (IQ), tending to ignore other aspects of neurodevelopment. Methodology: Here we present the relationship between breast feeding for at least 6 months with 373 neurocognitive outcomes measured from infancy through to late adolescence using data collected in the Avon Longitudinal Study of Parents and Children (ALSPAC). We first examined unadjusted regression associations with breast feeding at age 6 months. Where the unadjusted p-value was < 0.0001 (n = 152 outcomes), we adjusted for social and other factors. Results: This resulted in 42 outcomes with adjusted associations at p < 0.001. Specifically, these included associations with full-scale IQ at ages 8 and 15 years (adjusted mean differences [95% confidence interval (CI)] +4.11 [95% CI 2.83, 5.39] and +5.12 [95% CI 3.57, 6.67] IQ points, respectively, compared to not breastfeeding for 6 months). As well as the components of IQ, the other phenotypes that were strongly related to breast feeding for at least 6 months were measures of academic ability (reading, use of the English language and mathematics). In accordance with the literature, we show that children who are breast fed are more likely to be right-handed. The one association that has not been recorded before concerned aspects of pragmatic speech at 9 years where the children who had been breast fed were shown to perform more appropriately. Conclusions: We conclude that breast feeding for at least 6 months has beneficial effects on a number of neurocognitive outcomes that are likely to play a major part in the offspring’s future life course. We point out, however, the possibility that by using such stringent p-value criteria, other valid associations may have been ignored. Full article

(This article belongs to the Special Issue The Role of Nutrients in Child Neurodevelopment)

32 pages, 2283 KB

Open AccessReview

Diet and Depression During Peri- and Post-Menopause: A Scoping Review

by Alexandra M. Bodnaruc, Miryam Duquet, Denis Prud’homme and Isabelle Giroux

Nutrients 2025, 17(17), 2846; https://doi.org/10.3390/nu17172846 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: While the prevalence of depression increases during the peri- and post-menopausal periods, the potential of diet as both a modifiable risk factor and complementary treatment option has received limited research attention in this population. To address this gap, we conducted a [...] Read more.

Background/Objectives: While the prevalence of depression increases during the peri- and post-menopausal periods, the potential of diet as both a modifiable risk factor and complementary treatment option has received limited research attention in this population. To address this gap, we conducted a scoping review aiming to map and synthesize the existing literature on diet and depression in peri- and post-menopause. Methods: Studies were identified through Medline, EMBASE, PsycINFO, CENTRAL, Web of Science, and Scopus. After deduplication in Covidence, two reviewers independently screened titles, abstracts, and full texts using predefined eligibility criteria. Data were extracted using standardized forms and presented in tables and figures. Methodological quality was assessed using the Cochrane RoB-2 for intervention studies and NHLBI tools for observational studies. Results: Thirty-eight studies met the inclusion criteria, including 29 observational and 9 interventional studies. Dietary patterns showed the most consistent associations with depressive symptoms, whereas findings for foods, nutrients, and other food components were inconsistent. Most observational studies had a moderate to high risk of bias, while over half of experimental studies were rated as low risk. Conclusions: Although limited by volume and poor methodological quality, existing evidence suggests that healthy diets may be protective against depressive symptoms in peri- and post-menopausal women, while unhealthy diets may increase risk. High-quality cohort studies and clinical trials are needed to guide future research and inform professionals working at the intersection of nutrition, psychiatry, and women’s health. Protocol registration: osf.io/b89r6. Full article

(This article belongs to the Special Issue The Role of Different Dietary Patterns on Anxiety and Depression)

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17 pages, 839 KB

Open AccessArticle

Caffeine Intake Mediates the Relationship Between Problematic Overstudying and Psychological Distress

by Oliwia Kosecka, Edyta Charzyńska, Stanisław K. Czerwiński, Agata Rudnik and Paweł A. Atroszko

Nutrients 2025, 17(17), 2845; https://doi.org/10.3390/nu17172845 (registering DOI) - 31 Aug 2025

Abstract

Background: Problematic overstudying has been conceptualized as an addictive behavior (study addiction) and an early form of work addiction. The majority of students showing compulsive studying behaviors experience chronic and high stress and symptoms of generalized anxiety disorder. Caffeine is a widely used [...] Read more.

Background: Problematic overstudying has been conceptualized as an addictive behavior (study addiction) and an early form of work addiction. The majority of students showing compulsive studying behaviors experience chronic and high stress and symptoms of generalized anxiety disorder. Caffeine is a widely used stimulant that enhances alertness and cognitive performance, especially under fatigue. University students, particularly those exhibiting problematic overstudying, may consume more caffeine to improve academic performance. Previous research has shown that caffeine consumption is positively associated with perceived stress and anxiety. This study examined the mediating role of caffeine consumption in the relationship between problematic overstudying and psychological distress (perceived stress, anxiety, and depression) among university students. Methods: Sample 1 consisted of 436 university students, and Sample 2 included 3421 students. The Bergen Study Addiction Scale, Perceived Stress Scale-4, and a measure of average daily caffeine consumption were used. Results: Results showed that caffeine consumption partially mediated the relationship between problematic overstudying and perceived stress. Students who study compulsively tended to consume more caffeine, which was, in turn, associated with higher perceived stress. This finding was replicated across both samples, and in the second, larger sample, caffeine intake also mediated between problematic overstudying and anxiety and depression. Conclusions: Excessive caffeine use among students who manifest problematic overstudying may increase their risk of developing, or aggravate existing, symptoms of anxiety or mood disorders. Limiting caffeine intake and promoting healthy alternatives, such as rest and recovery, is recommended to support mental health in this population. Full article

(This article belongs to the Section Nutritional Epidemiology)

15 pages, 878 KB

Open AccessArticle

Modulation of the Gut Microbiota by Nopalea cochenillifera (Prickly Pear Cactus) Contributes to Improved Lipid Metabolism and Immune Function

by Sayaka Yokoyama, Amane Kikuchi, Hideaki Takahashi, Hinako Ushimaru, Hibiki Yamaguchi, Chikako Yamada, Kotoyo Fujiki, Hana Kozai, Suzuno Ota, Tadashi Fujii, Yoshiki Hirooka, Takumi Tochio and Mamoru Tanaka

Nutrients 2025, 17(17), 2844; https://doi.org/10.3390/nu17172844 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Nopalea cochenillifera (L.) Salm-Dyck cladodes are rich in dietary fiber, polyphenols, and minerals, which are known to exert antioxidant and immunomodulatory effects. However, the mechanisms and active constituents have not been fully elucidated. In this study, we investigated the effects of [...] Read more.

Background/Objectives: Nopalea cochenillifera (L.) Salm-Dyck cladodes are rich in dietary fiber, polyphenols, and minerals, which are known to exert antioxidant and immunomodulatory effects. However, the mechanisms and active constituents have not been fully elucidated. In this study, we investigated the effects of continuous N. cochenillifera consumption on lipid metabolism, immune function, and the gut microbiota in mice. Methods: The feed was made using freeze-dried and powdered cladodes of N. cochenillifera. Male C57BL/6J mice were assigned to four groups: control diet (C), control diet plus 10% N. cochenillifera (CN), high-fat diet (FC), and high-fat diet plus 10% N. cochenillifera (FN). Results: Cactus supplementation reduced the body and liver weights that were elevated by the high-fat diet. Serum total cholesterol and free fatty acids were increased in the FC group compared with the C group, while cactus intake lowered these levels and enhanced fecal cholesterol excretion. Cactus consumption also elevated fecal total IgA and mucin contents. IL-4 expression in Peyer’s patches was significantly increased in the FN group compared with the FC group. Gut microbiota analysis showed significant differences in β-diversity, along with increased α-diversity and higher abundance of Lachnospiraceae, following cactus intake. Conclusions: These findings suggest that N. cochenillifera intake increases gut microbiota diversity, which enhances intestinal barrier function and thereby contributes to improved lipid metabolism and immune regulation. Full article

(This article belongs to the Special Issue Functional Foods and Sustainable Health (2nd Edition))

18 pages, 978 KB

Open AccessArticle

Oncologists’ Perspectives on Ketogenic Diets in Pediatric Brain Cancer: Potential, Challenges, and the Path Forward

by Hanan AlMutairi, Madhumita Dandapani, Khawar Siddiqui and Fiona McCullough

Nutrients 2025, 17(17), 2843; https://doi.org/10.3390/nu17172843 (registering DOI) - 31 Aug 2025

Abstract

Background/objectives: Treating pediatric brain tumors remains challenging due to the limitations of conventional therapies, which often damage healthy cells. Ketogenic diets (KDs)—high in fat and low in carbohydrates—have emerged as potential adjunct therapies by limiting glucose availability and offering ketones as an [...] Read more.

Background/objectives: Treating pediatric brain tumors remains challenging due to the limitations of conventional therapies, which often damage healthy cells. Ketogenic diets (KDs)—high in fat and low in carbohydrates—have emerged as potential adjunct therapies by limiting glucose availability and offering ketones as an alternative energy source proposed to hinder tumor growth. However, due to limited awareness, there is hesitancy to recommend KDs. Methods: This study assessed oncologists’ knowledge and perceptions in Saudi Arabia regarding KD use in pediatric brain cancer patients. A cross-sectional survey was conducted with 94 oncologists from five major Riyadh healthcare centers, examining their knowledge, safety concerns, feasibility, and perceived efficacy of KDs. Results: Results showed that 67% correctly identified the basic composition of KDs, though 43% were neutral about its safety. Concerns about malnutrition and side effects were common and 53% found adherence to the diet challenging in pediatric patients. While 48.9% believed KDs could improve outcomes when combined with standard therapies, many stressed the importance of tailoring recommendations to individual medical conditions, including patient age, treatment stage, and overall nutritional status. Participants’ responses revealed variability in knowledge and perception levels regarding KDs, with consultants and internationally educated oncologists generally expressing more favorable views compared to fellows, who showed greater hesitancy. Conclusions: This study shows a mixed level of knowledge and perceptions among oncologists, reflecting a lack of consensus about KDs’ safety, feasibility, and potential benefits. These findings suggest the need for further education, clearer guidelines, and interdisciplinary collaboration to support informed decision-making, particularly in the local context. Full article

(This article belongs to the Section Pediatric Nutrition)

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20 pages, 910 KB

Open AccessReview

An Integrated Nutritional and Physical Activity Approach for Osteosarcopenia

by Edoardo Mocini, Ludovica Cardinali, Olivia Di Vincenzo, Antimo Moretti, Carlo Baldari, Giovanni Iolascon and Silvia Migliaccio

Nutrients 2025, 17(17), 2842; https://doi.org/10.3390/nu17172842 (registering DOI) - 31 Aug 2025

Abstract

Osteoporosis is a skeletal disorder characterized by decreased bone strength, which leads to an increased risk of developing fractures. Interestingly, this metabolic disorder is often related to sarcopenia, defined as decreased muscle mass, strength, and function. These two conditions appear to be closely [...] Read more.

Osteoporosis is a skeletal disorder characterized by decreased bone strength, which leads to an increased risk of developing fractures. Interestingly, this metabolic disorder is often related to sarcopenia, defined as decreased muscle mass, strength, and function. These two conditions appear to be closely connected, leading to a clinical condition named osteosarcopenia (OS). Aging may explain the link between muscle and bone loss through genetic, mechanical, endocrine, and nutritional factors. Further, aging increases the amount of adipose tissue, often due to sedentary behavior and unbalanced nutritional pattern, leading to a clinical condition defined as osteosarcopenic obesity, characterized by concurrent obesity, sarcopenia, and osteoporosis, where each condition exacerbates the others. Moreover, sarcopenia leads to decreased physical (PA) activity, worsening skeletal homeostasis, and creating a vicious cycle, which increases falls, fracture risk, and disability. This review underscores the importance of a systemic approach, focusing on nutritional therapy integrated with PA and, eventually, pharmacological interventions to efficiently manage (OS). Full article

(This article belongs to the Special Issue Nutrition 3.0: Between Tradition and Innovation)

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19 pages, 652 KB

Open AccessReview

The Role of Hydration in Children and Adolescents—A Theoretical Framework for Reviewing Recommendations, Models, and Empirical Studies

by Marek Zborowski and Magdalena Skotnicka

Nutrients 2025, 17(17), 2841; https://doi.org/10.3390/nu17172841 (registering DOI) - 31 Aug 2025

Abstract

Proper hydration is essential for maintaining homeostasis and the effective functioning of physiological systems, including the nervous and circulatory systems. During adolescence, a period characterized by rapid somatic growth, hormonal maturation, and increased physical and mental activity, the demand for water increases significantly. [...] Read more.

Proper hydration is essential for maintaining homeostasis and the effective functioning of physiological systems, including the nervous and circulatory systems. During adolescence, a period characterized by rapid somatic growth, hormonal maturation, and increased physical and mental activity, the demand for water increases significantly. Hydration affects not only the health of young people, but also their cognitive abilities, concentration, mood, and general well-being. Despite clear recommendations from institutions such as EFSA and IOM regarding daily fluid intake, numerous studies indicate that a significant proportion of young people do not achieve the recommended level of hydration. The school environment is particularly worrying, as young people spend a significant part of their day there, and the availability of water, health knowledge, and social conditions may contribute to dehydration or promote unhealthy choices (e.g., sweetened drinks). The aim of this article is to review the current state of knowledge on the importance of hydration in school-age adolescents. The physiological basis of hydration, the impact of insufficient fluid intake on the functioning of the young body, current guidelines, as well as the results of selected epidemiological studies and obstacles to ensuring optimal hydration in the school environment are discussed. Full article

(This article belongs to the Section Pediatric Nutrition)

21 pages, 2842 KB

Open AccessSystematic Review

Impact of Arabinoxylan Consumption on Glycemic Control: A Systematic Review and Meta-Analysis of Preclinical and Clinical Studies

by Yujing Xu, Yuxin Liang and Jung Eun Kim

Nutrients 2025, 17(17), 2840; https://doi.org/10.3390/nu17172840 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Arabinoxylan (AX) has shown potential benefits in glycemic control; however, findings remain inconclusive. This systematic review and meta-analysis aimed to assess the impact of AX intake on glycemic control in preclinical and clinical studies. Methods: A database search was conducted [...] Read more.

Background/Objectives: Arabinoxylan (AX) has shown potential benefits in glycemic control; however, findings remain inconclusive. This systematic review and meta-analysis aimed to assess the impact of AX intake on glycemic control in preclinical and clinical studies. Methods: A database search was conducted in PubMed, Embase, Cochrane Library, and CINAHL. A total of 133 studies were included for systematic review and extracted data from 46 clinical studies and 25 preclinical studies were further analyzed for meta-analysis. Results: The AX consumption improved overall postprandial glycemic control in clinical studies, as evidenced by reductions in glucose iAUC (SMD: −0.41; 95% CI: [−0.57, −0.25]), insulin iAUC (SMD: −0.28; 95% CI: [−0.44, −0.12]), glucose iPeak (SMD: −0.52; 95% CI: [−0.80, −0.25]), and insulin iPeak (SMD: −0.24; 95% CI: [−0.41, −0.06]) compared to the control. For chronic glycemic control, fasting glucose (Hedges’ g: −1.18; 95% CI: [−1.56, −0.80]), insulin (Hedges’ g: −1.07; 95% CI: [−1.92, −0.23]), HbA1c (Hedges’ g: −2.93; 95% CI: [−5.48, −0.38]), and HOMA-IR (Hedges’ g: −2.44; 95% CI: [−3.66, −1.22]) reduced in preclinical studies, while improvements were limited to fasting glucose (MD: −0.10; 95% CI: [−0.16, −0.03]) in clinical studies. Subgroup analyses revealed that AX exerted a greater glycemic-lowering effect in metabolically impaired animals and individuals compared to healthy counterparts. Furthermore, extracted AX was found to be more effective than intrinsic AX in optimizing glycemic control. Conclusions: The consumption of AX improves glycemic control, particularly in metabolically impaired animals and human participants. Moreover, the benefit appears more pronounced with extract AX interventions. Full article

(This article belongs to the Special Issue Recent Advances in Dietary Polysaccharides for Human Health and Diseases)

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16 pages, 873 KB

Open AccessArticle

Vitamin D Status and Response to Supplementation as Predictive Factors for Early Remission in Polymyalgia Rheumatica: A Retrospective Longitudinal Investigation

by Elvis Hysa, Serena Balito, Giulia Davoli, Elisa Caratto, Giulia Bernardi, Emanuele Gotelli, Rosanna Campitiello, Carmen Pizzorni, Sabrina Paolino, Alberto Sulli, Vanessa Smith and Maurizio Cutolo

Nutrients 2025, 17(17), 2839; https://doi.org/10.3390/nu17172839 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Polymyalgia rheumatica (PMR) is a relatively common inflammatory rheumatic disease of the elderly. The role of vitamin D remains unclear in this condition. The endpoints of this study were to assess 25-hydroxyvitamin D [25(OH)D] serum concentrations in PMR patients with active disease [...] Read more.

Background/Objectives: Polymyalgia rheumatica (PMR) is a relatively common inflammatory rheumatic disease of the elderly. The role of vitamin D remains unclear in this condition. The endpoints of this study were to assess 25-hydroxyvitamin D [25(OH)D] serum concentrations in PMR patients with active disease compared to elderly controls and to determine if baseline levels or changes following supplementation [delta 25-hydroxyvitamin D, Δ25(OH)D] were associated with improved clinical outcomes. Methods: In this retrospective, case–control study, 29 PMR patients (55% males, 75.24 ± 9.6 years old, disease duration of 3.8 ± 3 months) were included, meeting the 2012 EULAR/ACR classification criteria, with 29 age- and sex-matched controls without systemic inflammatory rheumatic diseases. We assessed demographic, clinical and laboratory features for PMR patients, including baseline 25(OH)D serum concentrations, disease activity (polymyalgia rheumatica activity score), and serum inflammatory biomarkers. A subgroup of them (n = 25) was followed longitudinally, for an average period of 21.1 ± 17.7 months, to evaluate the association between Δ25(OH)D and clinical outcomes at follow-up using multivariate logistic regression. Results: Although lower than the normal reference values, baseline 25(OH)D concentrations did not differ significantly between PMR patients and controls (21.6 ± 9.2 vs. 22.7 ± 11.3 ng/mL, p = 0.66) and did not predict long-term clinical outcomes. However, after only 3 months of supplementation, the increase in 25(OH)D concentrations was significantly associated with a remission status, and patients in remission showed a significant increase in 25(OH)D compared to those with persistent disease activity (+22.02 vs. +1.33 ng/mL, respectively; p = 0.044). Notably, in the multivariate model, this Δ25(OH)D was the strongest independent predictor of remission (OR = 2.89; 95% CI [1.60–4.11]), an effect independent of prednisone dosage prescribed at first visit (p = 0.32) and glucocorticoid exposure at third month (p = 0.12). Conclusions: Individual’s response of PMR patients to supplementation of vitamin D seems to be a robust independent predictor of early clinical remission achievement. Interestingly, optimizing vitamin D supplementation based on individual responsiveness may represent a valuable adjunctive strategy in PMR management. Full article

(This article belongs to the Special Issue Micronutrients and Bioactive Molecules: Their Development, Interaction, and Impact on Human Health)

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27 pages, 1140 KB

Open AccessReview

Protective Role of Key Micronutrients in Chemotherapy-Induced Organ Toxicity: A Comprehensive Review of Mechanistic Insights and Clinical Implications

by Ioannis Konstantinidis, Sophia Tsokkou, Eleni Gavriilaki, Georgios Delis and Theodora Papamitsou

Nutrients 2025, 17(17), 2838; https://doi.org/10.3390/nu17172838 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Systemic toxicities to key organs like the heart, liver, and kidneys impair the efficacy of chemotherapy in cancer treatment. These toxicities are caused by oxidative stress, inflammation, mitochondrial malfunction and ferroptosis, causing clinical morbidity and possibly impaired adherence to treatment. This review, [...] Read more.

Background/Objectives: Systemic toxicities to key organs like the heart, liver, and kidneys impair the efficacy of chemotherapy in cancer treatment. These toxicities are caused by oxidative stress, inflammation, mitochondrial malfunction and ferroptosis, causing clinical morbidity and possibly impaired adherence to treatment. This review, also, examines how magnesium, selenium, zinc and vitamin D protect against chemotherapy-induced cardiotoxicity, hepatotoxicity and nephrotoxicity. Methodology: A complete literature search of PubMed (MEDLINE), Scopus, Cochrane Library and Embase was used to synthesize data till 29 June 2025. Studies included randomized and non-randomized trials, cohort studies, case series (≥3 patients), and relevant systematic reviews. To contextualize pathways, preclinical in vivo and in vitro studies were studied independently. Patients undergoing systemic chemotherapy and magnesium, selenium, zinc or vitamin D therapies were eligible. Supplementation’s safety and organ-specific toxicity were investigated. Results: Magnesium protected against cisplatin-induced nephrotoxicity via modulating renal transporters and oxidative defenses across chemotherapy regimens. Selenium supplementation has strong antioxidant and anti-inflammatory characteristics, especially in avoiding cardiac and hepatic injury, although its nephroprotective potential was formulation-dependent. Zinc’s activity was connected to metallothionein-mediated redox stabilization, inflammatory regulation, and cardiac and hepatic resilience. Vitamin D and its analogs reduced cardiotoxicity and nephrotoxicity through mitochondrial preservation and immunomodulatory signaling. Conclusions: To date, magnesium, selenium, zinc, and vitamin D have been shown to reduce chemotherapy-related organ toxicities. Preclinical studies are promising, but randomized clinical trials are needed to prove therapeutic effectiveness and oncologic safety. Full article

(This article belongs to the Section Micronutrients and Human Health)

21 pages, 3847 KB

Open AccessArticle

Individual and Combined Effects of Medium- and Long-Chain Triacylglycerol and 2′-Fucosyllactose on Small Intestinal Morphology, Barrier Function, and Gut Microbiota in Growing C57BL/6 Mice

by Xinyuan Jin, Mengfan Shen, Mengdi Zhang, Haoqi Chen, Yufeng Jin, Yupeng Zeng, Zhijun Pan, Ziling Wang, Pan Wang, Yuting Yang, Zhiyuan Yan, Huilian Zhu and Dan Li

Nutrients 2025, 17(17), 2837; https://doi.org/10.3390/nu17172837 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Medium- and long-chain triacylglycerol (MLCT) and 2′-fucosyllactose (2′-FL) are functional ingredients abundant in human milk; however, their effects on small intestinal development and health remain largely unknown, and no research has explored their potential combined effects. Methods: In this study, growing C57BL/6 [...] Read more.

Background/Objectives: Medium- and long-chain triacylglycerol (MLCT) and 2′-fucosyllactose (2′-FL) are functional ingredients abundant in human milk; however, their effects on small intestinal development and health remain largely unknown, and no research has explored their potential combined effects. Methods: In this study, growing C57BL/6 mice (3 weeks old) were fed diets without or with 2.5 g/100 g of MLCT, 2′-FL, or the combination (MLCT + 2′-FL; 5:1) for 21 days. Body weight, major organ indices, small intestinal morphology-related indicators (small intestinal length, villus height, crypt depth, villus height/crypt depth (V/C) ratio, and epithelial cell proliferation), and intestinal barrier function markers (goblet cell and Paneth cell count, protein expression of ZO-1 and occludin, and levels of sIgA and LPS) were measured. Results: In addition to the shared promotion of epithelial cell proliferation, MLCT intervention raised villus height and crypt depth, while 2′-FL intervention elevated Paneth cell count and sIgA levels. Notably, MLCT + 2′-FL intervention offered additional advantages (increasing the V/C ratio, goblet cell count, and expression of ZO-1 and occludin) without affecting crypt depth. 16S rRNA sequencing analysis of cecal contents revealed that all three interventions mainly affected beta diversity rather than alpha diversity, and enriched differentially abundant bacterial taxa: Erysipelotrichaceae, Faecalibaculum, UBA1819, and Faecalitalea in the MLCT group; Enterobacteriaceae, Escherichia, and Allobaculum in the 2′-FL group; Bifidobacterium, Romboutsia, Clostridia, and several other bacterial taxa in the MLCT + 2′-FL group. Conclusions: These results indicate that MLCT and 2′-FL interventions alone appear to provide different benefits for small intestinal development, and their combination may confer more comprehensive advantages. Full article

(This article belongs to the Section Prebiotics and Probiotics)

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27 pages, 1171 KB

Open AccessReview

A Narrative Hypothesis: The Important Role of Gut Microbiota in the Modulation of Effort Tolerance in Endurance Athletes

by Jesus Álvarez-Herms, Martin Burtscher, Francisco Corbi, Adriana González and Adrián Odriozola

Nutrients 2025, 17(17), 2836; https://doi.org/10.3390/nu17172836 (registering DOI) - 31 Aug 2025

Abstract

Background: Regulating sensations of fatigue and discomfort while performing maximal endurance exercise becomes essential for making informed decisions about persistence and/or failure during intense exercise. Athletes with a higher effort capacity have competitive advantages over those with a lower one. The microbiota–brain axis [...] Read more.

Background: Regulating sensations of fatigue and discomfort while performing maximal endurance exercise becomes essential for making informed decisions about persistence and/or failure during intense exercise. Athletes with a higher effort capacity have competitive advantages over those with a lower one. The microbiota–brain axis is a considered the sixth sense and a modulator of the host’s emotional stability and physical well-being. Objectives: This narrative review aims to explore and evaluate the potential mechanisms involved in regulating perceptions during endurance exercise, with a focus on the possible relationship between the gut microbiota balance and the neural system as an adaptive response to high fatigue chronic exposure. Methods: Electronic databases (PubMed, Web of Science, Google Scholar, and Scopus) were used to identify studies and hypotheses that had documented predefined search terms related to endurance exercise, gut microbiota, the central nervous system, pain, discomfort, fatigue, and tolerance to effort. Results: This narrative review shifts the focus concerning the symbiotic relationship between the gut microbiota, the vagus nerve, the central/enteric nervous system, and the regulation of afferences from different organs and systems to manage discomfort and fatigue perceptions during maximal physical effort. Consequently, the chronicity supporting fatigued exercise and nutritional stimuli could specifically adapt the microbiota–brain connection through chronic efferences and afferences. The present hypothesis could represent a new focus to be considered, analysing individual differences in tolerating fatigue and discomfort in athletes supporting conditions of intense endurance exercise. Conclusions: A growing body of evidence suggests that the gut microbiota has rapid adaptations to afferences from the brain axis, with a possible relationship to the management of fatigue, pain, and discomfort. Therefore, the host–microbiota relationship could determine predisposition to endurance performance by increasing thresholds of sensitive afferences perceived and tolerated. A richer and more diverse GM of athletes in comparison with sedentary subjects can improve the bacteria-producing metabolites connected to brain activity related with fatigue. The increase in fatigue thresholds directly improves exercise performance, and the gut–brain axis may contribute through the equilibrium of metabolites produced for the microbiota. Full article

(This article belongs to the Special Issue Physical Exercise and Nutrition on the Gastrointestinal Pathophysiology)

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26 pages, 1536 KB

Open AccessReview

Generalized Anxiety Disorder and Obesity: Overlapping Neuroendocrine, Metabolic, and Behavioral Pathways

by Agnieszka Dymek, Magdalena Zielińska, Anna Englert-Bator, Katarzyna Dereń and Edyta Łuszczki

Nutrients 2025, 17(17), 2835; https://doi.org/10.3390/nu17172835 (registering DOI) - 31 Aug 2025

Abstract

Background/Objectives: Generalized anxiety disorder (GAD) is one of the most commonly diagnosed anxiety disorders in primary care. The global lifetime prevalence of GAD is estimated at 3.7%, ranging from 1.6% in low-income countries to 5.0% in high-income countries, underscoring its widespread impact. [...] Read more.

Background/Objectives: Generalized anxiety disorder (GAD) is one of the most commonly diagnosed anxiety disorders in primary care. The global lifetime prevalence of GAD is estimated at 3.7%, ranging from 1.6% in low-income countries to 5.0% in high-income countries, underscoring its widespread impact. Given the frequent co-occurrence of GAD with obesity, this association has important clinical implications, particularly for screening, prevention, and treatment strategies. The aim of this review is to identify potential biological mechanisms linking obesity and GAD, summarize the current state of knowledge in this area, and highlight existing research gaps, as well as directions for future research. Methods: This narrative review is based on the literature published between 2015 and 2025 concerning the co-occurrence of GAD and obesity, with a focus on potential shared mechanisms including HPA axis dysregulation, chronic inflammation, oxidative stress, insulin resistance, gut–brain axis and microbiota dysbiosis, sleep disturbance, and maladaptive eating behaviors. Results/Conclusions: A growing body of evidence suggests an important, albeit still ambiguously defined, relationship between obesity and GAD. GAD and obesity may reinforce each other, leading to a mutually reinforcing relationship. Despite growing interest, high-quality prospective and interventional studies focusing specifically on GAD are lacking. A potentially effective therapeutic approach should be integrated and multidisciplinary, combining psychological, pharmacological, and lifestyle interventions. It may also be beneficial for clinicians to consider routine assessment of anxiety in patients with obesity and, conversely, to monitor metabolic risk in individuals with GAD. Such an approach, targeting both mental and metabolic domains, holds promise for improving outcomes. Full article

(This article belongs to the Special Issue Impact of Eating Disorders and Obesity on Quality of Life and Mental Health)

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35 pages, 825 KB

Open AccessSystematic Review

Unraveling the Role of Foods on Chronic Anti- and Pro-Inflammatory Cytokines: A Systematic Review of Chronic Dietary Intervention Trials in Humans

by Veronica D’Antonio, Marina Ramal-Sanchez, Chiara Bravo-Trippetta, Elena Corvaglia, Mauro Serafini and Donato Angelino

Nutrients 2025, 17(17), 2834; https://doi.org/10.3390/nu17172834 (registering DOI) - 30 Aug 2025

Abstract

Background/Objectives: Unbalanced diets contribute to a rise in low-grade systemic inflammation, a risk factor for metabolic diseases. The aim of this study is to systematically review evidence from chronic intervention studies to understand the role of foods in modulating inflammatory responses in humans. [...] Read more.

Background/Objectives: Unbalanced diets contribute to a rise in low-grade systemic inflammation, a risk factor for metabolic diseases. The aim of this study is to systematically review evidence from chronic intervention studies to understand the role of foods in modulating inflammatory responses in humans. Methods: A literature search was conducted on PubMed using specific keywords. Risk of bias was assessed using Cochrane guidelines. Inclusion criteria required chronic dietary intervention studies measuring cytokine levels in humans. Results: In the 75 studies selected, results revealed extremely high variability both in outcomes, study design, and participant selection criteria. Studies with fruits and vegetables showed a reduction in circulating cytokine levels and/or an increase in anti-inflammatory cytokines in 80% of studies (8/10), followed by fish (78%; 7/9), dairy (67%; 4/6), cereals (64%; 7/11), and oils (57%; 4/7). Beverages and hot beverages showed a decrease in circulating cytokines in 50% of cases (10/20 and 4/8, respectively). An increase in pro-inflammatory cytokines was observed in dietary interventions with beverages. As further findings, we also observed greater effectiveness from fruits and vegetables (87.5%; 7/8), fish (75%; 6/8), and cereals (62.5%; 5/8) when studies were conducted in subjects with pathologies or risk factors. Conclusions: Fruits and vegetables, fish, and cereals reduce systemic inflammation mainly in subjects with pathologies or risk factors. However, the limited number of studies do not allow us to draw solid conclusions on individual foods. Standardized dietary intervention trials are urgently needed to understand the role of foods in modulating inflammatory responses and to deliver findings to the general public. Full article

(This article belongs to the Special Issue Nutrition 3.0: Between Tradition and Innovation)

23 pages, 1752 KB

Open AccessSystematic Review

Comparing the Effects of Dairy and Soybean on Bone Health in Women: A Food- and Component-Level Network Meta-Analysis

by Li You, Langrun Wang, Shiwen Zhou, Yiran Guan, Yan Liu, Ruixin Zhu, Huiyu Chen, Jie Guo, Keji Li, Xingyu Bao, Haotian Feng, Ignatius M. Y. Szeto, Jian He, Ran Wang and Jingjing He

Nutrients 2025, 17(17), 2833; https://doi.org/10.3390/nu17172833 (registering DOI) - 30 Aug 2025

Abstract

Background/Objectives: Dairy and soybean are important potential dietary sources of bone health. However, their comparative effectiveness and the role of specific components remain unclear. In this network meta-analysis (NMA), we aimed to compare the effects of various dairy and soy products (food [...] Read more.

Background/Objectives: Dairy and soybean are important potential dietary sources of bone health. However, their comparative effectiveness and the role of specific components remain unclear. In this network meta-analysis (NMA), we aimed to compare the effects of various dairy and soy products (food level) and their key bioactive components (component level) on bone health in healthy women. Methods: We systematically searched PubMed, Embase, Cochrane Library, and Web of Science (up to 28 February 2025) for randomized controlled trials. A frequentist random-effects NMA was used to compare interventions for lumbar spine (LS) and total body (TB) bone mineral density (BMD) and bone turnover markers [osteocalcin (OC), deoxypyridinoline (DPD)]. Mean differences (MDs) and 95% confidence intervals were pooled. Interventions were ranked using the surface under the cumulative ranking curve (SUCRA). Results: Sixty RCTs involving 6284 participants (mean age: 54.2 years) were included. At the food level, no dairy or soy interventions significantly improved outcomes versus control, although milk + yogurt ranked numerically highest based on SUCRA values. At the bioactive-component level, the combination of casein + whey protein (MD 0.04 g/cm², 95% CI 0.01–0.06) and soybean protein (MD: 0.03 g/cm², 95% CI: 0.01–0.05) significantly increased TB BMD. Whey protein alone (SUCRA 74.4% for LS BMD) and casein + whey protein (SUCRA 86.3% for TB BMD and 75.9% for DPD) were among the highest-ranked interventions for bone health. Conclusions: The combination of milk and yogurt may be relatively promising among dairy products for bone health. Whey protein appears to be a key bioactive component beneficial for women’s bone health. Full article

(This article belongs to the Special Issue Dietary Interventions for the Prevention and Treatment of Osteoporosis)

24 pages, 602 KB

Open AccessArticle

Associations Between Diet, Oral Health, and General Development in Romanian School-Age Children

by Ana-Gabriela Seni, Andreea Sălcudean, Ramona-Amina Popovici, Dora-Mihaela Cîmpian, Teodora Olariu, Iustin Olariu, Mariana Păcurar, Andreea Mihaela Kiș, Silviu-Constantin Bădoiu, Viorel Jinga, Alexandru Blidaru, Silviu-Ionel Dumitrescu, Ramona-Camelia Anculia, Norina Forna, Liana Todor and Monica Tarcea

Nutrients 2025, 17(17), 2832; https://doi.org/10.3390/nu17172832 (registering DOI) - 30 Aug 2025

Abstract

Background: The prevalence of dental caries has increased among children, largely due to nutritional habits or inadequate access to dental care. The present study aimed to investigate the prevalence of dental caries associated with various factors that lead to their appearance, such as [...] Read more.

Background: The prevalence of dental caries has increased among children, largely due to nutritional habits or inadequate access to dental care. The present study aimed to investigate the prevalence of dental caries associated with various factors that lead to their appearance, such as food intake and body composition, among Romanian school-age children from two counties, Bistriţa Năsăud and Mureş. Methods: This cross-sectional study included 1100 children aged 6–10 years from two Romanian counties. Dental caries experience was assessed using the DMFT and dmft indices based on WHO criteria. Dietary intake and oral health behaviors were evaluated through a food frequency questionnaire completed by parents. Associations between dietary variables and dental caries were assessed using independent sample t-tests and Mann–Whitney U tests. A binary logistic regression model was used to estimate the likelihood of caries in the permanent dentition (DMFT > 0), with covariates including county, parental education, daily sugar intake, consumption of dairy products, body mass index (BMI), and waist-to-height ratio. Results: It was found that the children who daily consume cheese recorded a DMFT value lower than the children who did not consume (p < 0.05). Moreover, those who consume sugary foods recorded higher values of DMFT as compared with those who did not (p < 0.05). The body weight, BMI, and waist circumference are positively correlated with DMFT, but negatively with dmft (p < 0.05). The overall prevalence of caries was 79.8% in primary dentition and 63.6% in permanent dentition, with slightly higher rates observed in Bistriţa-Năsăud County compared to Mureş. It seems that the predictors of the caries’s presence among children include the mother’s education level, sugar intake frequency, and body fat ratio. Conclusions: Based on significant associations found, one can affirm that the dental caries of the Romanian school-age children is due to sugary food intake and body composition. In addition, the direct relationship between dental caries and childhood obesity showed through the correlation of BMI and dental health indices denotes that school-age children should reduce sugary foods and increase dairy products. Full article

(This article belongs to the Special Issue Nutrition in Children's Growth and Development)

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14 pages, 1303 KB

Open AccessArticle

Non-Targeted Metabolomic Analysis of Ethanol Extract of Propolis and Its Anti-Inflammatory Effects in LPS-Induced BV2 Microglial Cells via the TLR4 Signaling Pathway

by Xiaolan Xu, Chunxia Li, Yuxuan Zhu, Shuangshuang Zhao, Fangjing Wu, Qian He, Lizhen Wei, Xinle Duan and Jianghong Li

Nutrients 2025, 17(17), 2831; https://doi.org/10.3390/nu17172831 (registering DOI) - 30 Aug 2025

Abstract

Propolis contains abundant flavonoid and phenolic compounds, whose composition and concentration vary significantly in different geographical origins, thereby affecting its bioactive properties including anti-inflammatory, antioxidant, and antimicrobial activities. In this study, the flavonoid and phenolic content in the ethanol extract of propolis (EEP) [...] Read more.

Propolis contains abundant flavonoid and phenolic compounds, whose composition and concentration vary significantly in different geographical origins, thereby affecting its bioactive properties including anti-inflammatory, antioxidant, and antimicrobial activities. In this study, the flavonoid and phenolic content in the ethanol extract of propolis (EEP) from Henan (HN) and Shandong (SD) provinces was quantitatively analyzed, and the results showed that concentrations of both bioactive components in HN were slightly higher than those in SD. The non-targeted metabolomics technology was further employed to analyze the components of EEP, and a total of 10683 metabolites were detected. In the comparison between the samples of HN and SD, there were a total of 1436 differential metabolites, with 553 decreased and 883 increased in the HN sample. Among them, there were 205 differential metabolites related to flavonoids and phenols, with 108 decreased and 97 increased in the HN sample. However, a greater number of carboxylic acids and derivatives, fatty derivatives and organooxygen metabolites were found at higher relative levels in the HN sample. As a result, the EEP of the HN sample was selected to investigate its inhibitory effect on inflammation in lipopolysaccharide (LPS)-induced BV2 microglia cells. The results showed that LPS promoted the M1 polarization of BV2 microglia. However, treatment with EEP at concentrations of 10 µg/mL, 5 µg/mL, and 2.5 µg/mL could partially restore the cell morphology to its non-activated state. Meanwhile, LPS stimulation increased the protein levels of IL-1β, IL-6 and TNF-α significantly, as well as the relative gene expression levels of IL-1β, IL-6, TNF-α, COX-2, iNOS and TLR4. After treatment with the EEP, the expression levels of these three proteins and six genes were significantly decreased. These findings revealed that EEP effectively inhibited the M1 polarization of LPS-induced BV2 cells and decreased the expression of inflammatory factors, indicating its potential as a therapeutic agent for neuroinflammation. Full article

(This article belongs to the Special Issue Nutritional and Health Value of Natural Products From Medicinal Plants on Human Diseases)

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12 pages, 301 KB

Open AccessArticle

Patient and Family Perspectives on Integrated Transitional Care for Anorexia Nervosa in Mantova, Italy

by Debora Bussolotti, Giovanni Barillà, Antonia Di Genni, Martina Comini, Alberto Gallo, Mariateresa Torre, Laura Orlando, Beatrice Mastrolorenzo, Eva Corradini, Barbara Bazzoli, Francesco Bonfà, Andrea Mora, Luca Pasqualini, Elisa Mariantoni, Alessandro Cuomo, Despoina Koukouna and Paola Accorsi

Nutrients 2025, 17(17), 2830; https://doi.org/10.3390/nu17172830 (registering DOI) - 30 Aug 2025

Abstract

Background/Objectives: The child and adolescent mental health service (CAMHS) hand-over to adult mental health service (AMHS) remains an ongoing shortfall in eating disorder (ED) treatment, typically in tandem with diagnostic drift, heightened suicide risk, and carer burn-out. We created one 14-to-25 Transition—ED track [...] Read more.

Background/Objectives: The child and adolescent mental health service (CAMHS) hand-over to adult mental health service (AMHS) remains an ongoing shortfall in eating disorder (ED) treatment, typically in tandem with diagnostic drift, heightened suicide risk, and carer burn-out. We created one 14-to-25 Transition—ED track within our own unit, where a single multidisciplinary team continuously follows each patient and family across the CAMHS–AMHS boundary (via weekly joint paediatric and adult clinician meeting) without changing the individual psychotherapist, family therapist, or dietitian at the age 18 transition. We investigated the manner in which patients and parents perceive this model. Methods: A survey of two naturalistic parent cohorts—CAMHS (n = 16) and Transition—Adult arm (n = 15)—also joined, alongside the original group of young adults who had entered the programme during its set-up phase (n = 9). Here, the 14–25 pathway denotes one unified route of care across adolescence and young adulthood; the Transition—Adult arm is its ≥ 18-years component. All index patients had a primary DSM-5-TR diagnosis of restricting-type anorexia nervosa. Participants completed the Client Satisfaction Questionnaire-8 (CSQ-8; range 8–32) and four bespoke Continuity-of-Care items (1–4 Likert). Results: Overall, the caregivers in both cohorts were pleased (median CSQ-8 = 28.5 [CAMHS] vs. 27.0 [Transition]; p = 0.75). Continuity items were universally well rated across cohorts. Cohort parents reported a median of two unchanged core clinicians (i.e., the individual psychotherapist, the family therapist, or the dietitian), which was nonsignificantly positively correlated with CSQ-8 scores (ρ = 0.22). Early-group patients mirrored caregiver impressions (mean CSQ-8 = 27.0 ± 3.9). Conclusions: It is feasible and highly acceptable to both caregivers and anorexia nervosa young adults to have the same key staff and family-centred sessions over the 14-to-25 age span. Constrained by single-site study and small sample size, these preliminary data provide a rationale for wider implementation and controlled follow-up studies. Full article

(This article belongs to the Special Issue Impact of Eating Disorders and Obesity on Quality of Life and Mental Health)

22 pages, 3227 KB

Open AccessArticle

Dietary Tart Cherry and Fructooligosaccharides Promote Bone Health via the Gut Microbiota and Increased Bone Formation

by Pelumi Adedigba, John A. Ice, Sanmi E. Alake, Bethany Hatter, Proapa Islam, Ashlee N. Ford Versypt, Trina A. Knotts, Jerry Ritchey, Edralin A. Lucas and Brenda J. Smith

Nutrients 2025, 17(17), 2829; https://doi.org/10.3390/nu17172829 (registering DOI) - 30 Aug 2025

Abstract

Background/Objectives: Fructooligosaccharides (FOS) and dried tart cherry (TC) are examples of simple and complex (i.e., within a food matrix) prebiotics that have demonstrated promising osteoprotective activity. In this study, we examined how dietary supplementation with TC or FOS shapes the gut-bone axis to [...] Read more.

Background/Objectives: Fructooligosaccharides (FOS) and dried tart cherry (TC) are examples of simple and complex (i.e., within a food matrix) prebiotics that have demonstrated promising osteoprotective activity. In this study, we examined how dietary supplementation with TC or FOS shapes the gut-bone axis to promote bone accrual in young adult mice, and the role of the gut microbiota in mediating these responses. Methods: Studies were performed using 10-wk-old female C57BL/6 mice (n = 10–12/group) fed a control diet or control diet supplemented with 10% TC or FOS for 10 wks alone or in combination with an antibiotic/anti-fungal cocktail to suppress the gut microbiota. The bone phenotype was characterized by dual-energy X-ray absorptiometry, micro-computed tomography and static and dynamic bone histomorphometry. The gut-microbiota was profiled and short chain fatty acids (SCFA) were assessed based on 16S rRNA profiling and gas chromatographic techniques, respectively. Results: FOS and TC enhanced bone structure, with FOS yielding more pronounced benefits across cortical and trabecular compartments. These skeletal improvements with FOS occurred in the absence of systemic changes in bone turnover markers but were accompanied by increases in local bone formation, osteoblast and osteocyte numbers, and bone mineralization in the femur. Both diets altered gut microbiota composition and increased fecal concentrations of the most abundant SCFAs (i.e., acetate, propionate and butyrate), but the response was greater with FOS. Suppression of the gut microbiota and fecal SCFAs with the antibiotic/anti-fungal cocktail inhibited the effects of FOS and TC on cortical bone, but induced unexpected improvements in the trabecular bone. Conclusions: These findings demonstrate differential effects of simple and complex prebiotics on the gut-bone axis in young adult female mice and support a role for SCFA in the cortical bone response, but not in the trabecular bone response with this model of gut microbiota suppression. Full article

(This article belongs to the Section Prebiotics and Probiotics)

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