Table of Contents
Int. J. Mol. Sci., Volume 21, Issue 2 (January-2 2020) – 316 articles
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Cover Story (view full-size image) Targeted DNA methylation is a promising approach for the modulation of gene expression in basic [...] Read more. Targeted DNA methylation is a promising approach for the modulation of gene expression in basic research and in the clinic. However, it suffers from a lack of specificity, because often, off-target regions are also methylated. We have rationally designed different DNMT3A variants with reduced DNA binding and applied them for targeted methylation. Our data show that this leads to a pronounced improvement in the specificity of targeted DNA methylation, as indicated by a strongly reduced off-target methylation accompanied by an only mild reduction of on-target activity. These improved DNMT3A variants will advance the future applications of this emerging technology. View this paper.