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Hygiene

Hygiene is an international, peer-reviewed, open access journal on healthcare epidemiology, public health and environmentally-related hygiene, published quarterly online by MDPI.
We also welcome submissions with a focus on best practices that help maintain health and prevent the spread of diseases.

All Articles (168)

Background: Environmental surfaces are key reservoirs for pathogen transmission, with the survival of bacteria on fomites influenced by factors such as temperature, humidity, and microbial interactions. This study aimed to determine microbial surface contamination and to determine the antimicrobial resistance profile of bacteria isolated from the indoor surface where the presence of cockroaches was observed in households of the Greater Letaba Municipality (GLM), South Africa. Methods: Swab samples were collected from kitchen countertops and food storage areas with visible cockroach activity. Bacteria were isolated and identified using standard microbiological methods, and antimicrobial susceptibility testing (AST) was conducted with the Vitek® Automated 2 system. Results: Of the 120 samples collected, 82 (68%) showed bacterial growth, resulting in 190 isolates. The majority of isolates (93%) were Gram-negative, comprising Klebsiella, Pseudomonas, Enterobacter, Escherichia, Serratia, Stenotrophomonas, Pantoea, Raoultella, and Salmonella species, with 98% demonstrating multidrug resistance (MDR) to multiple antibiotics. Resistance was particularly high against gentamicin (94%), fluoroquinolones (88%) and amikacin (77%). Among Gram-positive isolates, all belonged to the Enterococcus species, with 22% being resistant to one or two of the tested antimicrobial agents and 78% exhibiting MDR. Conclusions: The study revealed a high prevalence of antibiotic resistance in both Gram-positive and Gram-negative bacteria isolated from household surfaces. The spread of antibiotic-resistant pathogens via environmental surfaces presents a significant risk to human health, safety, and well-being.

27 November 2025

The microbial landscape: prevalence of Gram-positive isolates on environmental surfaces in a household setting.

Assessing the Self-Reported Level of Food Hygiene Knowledge and Practices Among Cookery Teachers in Northern Philippines

  • Shareen Kate A. Gamiao,
  • Marie Dale R. Soriano and
  • Realyn Q. Salvador
  • + 6 authors

In the Philippine basic education system, particularly in Technology and Livelihood Education (TLE) Cookery classes, teachers are at the forefront of promoting proper food hygiene. However, systemic challenges such as the absence of standardized policies, outdated training, and lack of resources hinder their effectiveness. To address this gap, this study aimed to assess the food hygiene knowledge and practices of cookery teachers and provides localized evidence to address the lack of division-level hygiene policies. A descriptive research design supported by qualitative interviews was employed. In total, 69 junior and senior high school cookery teachers from three school divisions in Ilocos Norte participated. A researcher-made survey questionnaire and an interview guide were used to gather data, which were analyzed using descriptive and inferential statistics. Participant testimonies were integrated to enrich the quantitative findings. Results showed high levels of food hygiene knowledge (x¯= 3.48; Highly Competent) and practices (x¯= 3.80; Highly Competent). Despite these strengths, notable gaps were identified in technical areas such as temperature control, cold storage, and specific aspects of personal hygiene. Interviews highlighted the need for a formal institutional hygiene policy to support teachers’ implementation of safe food-handling practices. All computed p-values were below 0.01, indicating significant correlations between demographic variables and both knowledge and practices. The correlation values ranged from r = 0.039 to r = 0.342, suggesting weak to moderate positive relationships and indicating that hygiene behaviors are influenced by multiple factors rather than demographics alone. Based on the findings, the study recommends institutionalizing the proposed policy brief, providing adequate resources, and implementing continuous professional development for Cookery teachers. The study’s scope is limited to Northern Philippines.

7 December 2025

Background: The use of computer-aided design and manufacturing (CAD/CAM) technology is rapidly expanding in modern dentistry. However, differences may exist in educational experiences and utilization competencies between dental hygiene and dental technology students. Objectives: This study aimed to assess CAD/CAM educational exposure and competencies by major and grade level. Methods: A cross-sectional online survey was conducted with 464 undergraduate students in Korea. The questionnaire covered general characteristics, CAD/CAM education experience, and competencies in scanning, CAD design, CAM operation, and post-processing. Data were analyzed using descriptive statistics, independent-samples t-tests, and correlation analysis. Results: A total of 464 valid responses were analyzed. Dental technology students reported significantly higher experience with CAD/CAM education compared with dental hygiene students (p < 0.001). Scanning, CAD design, CAM operation, and post-processing competency were all significantly higher among dental technology students and senior (3–4 year) students compared with dental hygiene and junior (1–2 year) students (p < 0.001). CAD/CAM competencies varied significantly by major and grade level. Conclusions: These findings emphasize the need to strengthen CAD/CAM training in dentistry curricula and to promote interdisciplinary digital dentistry education to prepare practice-ready graduates for a digitized clinical environment.

5 December 2025

In Vitro Disinfection Efficacy Assay on Giardia duodenalis Cysts

  • Manuela Kirchner,
  • Cora Delling and
  • Arwid Daugschies

Background: The cysts of the protozoan parasite Giardia duodenalis, which targets a broad spectrum of hosts including humans, can withstand environmental conditions for months, making effective disinfectant measures crucial for minimizing the infection burden. Previous investigations concerning disinfection efficacy were based on cysts from fecal/water samples or animal models, which are either unfit for standardized procedures or related to ethical concerns. Methods: To perform standardized in vitro disinfectant testing, four different encystation protocols were compared firstly. The protocol with the highest efficacy in our hands (1.7 × 105 cysts per tube) was used for the production of cysts to establish a disinfectant assay. Therefore, it was used for the production of cysts to establish a dis-infectant efficacy assay. After incubation with a commercial disinfectant (ViPiBaX Giardien Ex®) or 30% hydrogen peroxide solution (H2O2) at 10 °C and room temperature, parasite cyst viability was evaluated by the yield of trophozoites obtained by the applied excystation protocol. Results: Only untreated Giardia cysts, which were used as a negative con-trol, released trophozoites. The protocol established for the evaluation of cyst viability delivered reproducible results and appeared suitable for testing the inactivation of cysts by chemical disinfection. Conclusions: Under the given conditions, the disinfectant ViPiBaX Giardien Ex® and H2O2 inactivated Giardia cysts.

25 November 2025

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Hygiene - ISSN 2673-947X