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Evaluating Dental Students’ Knowledge and Attitudes Toward Antisepsis and Infection Control: An Educational Intervention Study at a Public University Dental Department
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Respiratory Infections in Adults and Inequality: An Analysis of Deaths and Their Socioeconomic Determinants in Brazil
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Hand Hygiene in Greek Public Hospitals: Exploring Knowledge, Self-Reported Compliance, and the Impact of a Behavioral Economics-Based Nudge
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Bacterial Contamination of Public and Household Restrooms, and Implications for the Potential Risk of Norovirus Transmission
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Evaluation of Water Safety Plan Compliance in Italian Hospitals According to Legislative Decree 18/23 and Directive EU 2020/2184: A Cross-Sectional Study
Journal Description
Hygiene
Hygiene
is an international, peer-reviewed, open access journal on healthcare epidemiology, public health and environmentally-related hygiene, published quarterly online by MDPI. We also welcome submissions with a focus on best practices that help maintain health and prevent the spread of diseases.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within ESCI (Web of Science), Scopus and other databases.
- Journal Rank: CiteScore - Q2 (Immunology and Microbiology (miscellaneous))
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18.1 days after submission; acceptance to publication is undertaken in 4.6 days (median values for papers published in this journal in the first half of 2025).
- Recognition of Reviewers: APC discount vouchers, optional signed peer review, and reviewer names published annually in the journal.
- Journal Cluster of Healthcare Sciences and Services: Geriatrics, Journal of Ageing and Longevity, Healthcare, Hospitals, Hygiene, International Journal of Environmental Research and Public Health and Nursing Reports.
Latest Articles
Consumer Awareness of Microbial Contamination and Identification of Key Pathogenic Bacteria in Lip Cosmetic Testers
Hygiene 2025, 5(4), 47; https://doi.org/10.3390/hygiene5040047 - 8 Oct 2025
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Background: With the increasing popularity and diversification of cosmetic products, an expanding number of retail stores are providing in-store testers to allow consumers to try products before purchasing. However, growing concerns have been raised about microbial contamination and the associated risk of infection
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Background: With the increasing popularity and diversification of cosmetic products, an expanding number of retail stores are providing in-store testers to allow consumers to try products before purchasing. However, growing concerns have been raised about microbial contamination and the associated risk of infection due to the shared use of these testers. This study aimed to investigate consumer awareness and perceived susceptibility to microbial infection associated with the use of lip cosmetic testers, and to identify major pathogenic bacteria, thereby offering evidence to support the need for improved hygiene management practices. Methods: The survey examined the use of lip cosmetic testers and related side effects, as well as perceptions of product hygiene and microbial contamination. Awareness of microbial infection and consumer sensitivity to product safety were measured using a five-point Likert scale. Microorganisms were detected through PCR analysis of genomic DNA. Results: Among 134 respondents, 95% had previously used lip cosmetic testers, and 90% recognized the potential for microbial contamination. Sensitivity to product safety was not significantly associated with sociodemographic factors. However, susceptibility to microbial infection from tester use was significantly higher among participants of older age and with higher education levels. PCR analysis of lip cosmetic testers revealed frequent detection of Staphylococcus epidermidis and Pseudomonas aeruginosa. Other detected bacteria included Streptococcus oralis, Streptococcus salivarius, Streptococcus pneumoniae, and Escherichia coli, all of which are known to cause oral and respiratory infections. Conclusions: The analysis revealed that the majority of individuals who have used lip cosmetic testers expressed a high level of concern regarding product contamination and the potential for infection. Multiple pathogenic bacteria linked to oral and respiratory diseases were identified in the opened tester products. These findings underscore the necessity of establishing regulatory guidelines for the management of lip cosmetic testers and highlight the importance of enhancing consumer awareness regarding hygiene practices.
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Open AccessReview
The Mother—Infant Symbiosis: A Novel Perspective on the Newborn’s Role in Protecting Maternal Breast Health
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Darío de Jesús Guillén-Morales, Isabel Cruz-Cortés, Taurino Amilcar Sosa-Velazco and Alba Soledad Aquino-Domínguez
Hygiene 2025, 5(4), 46; https://doi.org/10.3390/hygiene5040046 - 3 Oct 2025
Abstract
Breastfeeding is a complex biological system and a bidirectional physiological dialogue in which the infant may contribute to maternal breast health. This review synthesizes current evidence, clearly separating established findings from emerging hypotheses, to examine the possible infant-driven mechanisms that influence hormonal and
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Breastfeeding is a complex biological system and a bidirectional physiological dialogue in which the infant may contribute to maternal breast health. This review synthesizes current evidence, clearly separating established findings from emerging hypotheses, to examine the possible infant-driven mechanisms that influence hormonal and immune homeostasis in the mammary gland. We evaluate how neonatal suckling coordinates interconnected hormonal reflexes and immune activity, and we explore the hypothesis that the retrograde flow of infant saliva to the breast tissue could activate maternal enzymatic defenses, particularly the xanthine oxidase and lactoperoxidase systems. We also consider the activation of antimicrobial peptides through direct contact at the nipple and areola, including cathelicidin and defensins, as well as the potential roles of fetal microchimerism and microbial transfer from the infant’s mouth in strengthening breast resilience. Although much of the evidence remains indirect and based on in vitro and animal models, the convergence of data supports a reformulated conceptual model that presents the infant as an active physiological partner rather than a passive recipient of milk. Recognizing this shift has important clinical implications for the prevention of inflammatory conditions such as mastitis, the improvement of breastfeeding support strategies, and the optimization of maternal and infant health outcomes. The review also identifies significant gaps in current knowledge and cautiously proposes hypotheses to explore these mechanisms. While preliminary, this framework offers an original perspective that may guide future research and open new paths in the study of human lactation biology.
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(This article belongs to the Section Food Hygiene and Safety)
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Open AccessSystematic Review
Oral Hygiene Protocols and Basic Oral Care in Cancer Patients: A Systematic Review
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Maria Cláudia Cuzzullin, Marcelo Ivander Andrade Wanderley, Maria Eduarda Pérez-de-Oliveira, Ana Gabriela Costa Normando, Anna Luíza Damaceno Araújo, Joab Cabral Ramos, Thaís Bianca Brandão, Joel B. Epstein, Alan Roger Santos-Silva and Ana Carolina Prado-Ribeiro
Hygiene 2025, 5(4), 45; https://doi.org/10.3390/hygiene5040045 - 1 Oct 2025
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Oral hygiene protocols and basic oral care (BOC) are paramount for patients undergoing cancer treatment, although based upon limited evidence from clinical trials. There are a range of oral care guidelines proposed in the literature mainly focusing on oral mucositis prevention. This systematic
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Oral hygiene protocols and basic oral care (BOC) are paramount for patients undergoing cancer treatment, although based upon limited evidence from clinical trials. There are a range of oral care guidelines proposed in the literature mainly focusing on oral mucositis prevention. This systematic review aimed to define the best procedures and practices in the scope of oral hygiene protocols and BOC interventions for the prevention and/or treatment of oral complications in patients under cancer treatment. This systematic review was conducted according to the PRISMA guidelines, and the methods were previously established and registered in PROSPERO (CRD42022319455). A systematic search was completed in six databases (PubMed, Scopus, Web of Science, LILACS, and the Cochrane Library, and gray literature). Eleven studies were included in the qualitative analysis including non-randomized (n = 1) and randomized clinical trials (n = 6), and cohort studies (n = 4). The included studies showed the effectiveness of soft brushes for daily oral hygiene with topical fluoride, and the use of mouthwashes with 0.12% chlorhexidine, doxepin, and benzydamine and saline solutions, as well as dental scaling/prophylaxis in decreasing the severity of oral toxicities. Although not well-explored by well-designed clinical studies, BOC protocols may be effective in prevention oral toxicities from cancer therapy and are supported in multiple guidelines. This review synthesized available evidence on BOC in head and neck cancer patients, focusing on the effectiveness of hospital-based dental protocols during cancer treatment.
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Open AccessArticle
Assessment of Knowledge and Attitudes of Healthcare Personnel Towards Artificial Intelligence Technologies in Greece: A Survey Study
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Dimitris Karaferis, Dimitra Balaska, Maria Eleni Karaferi and Yannis Pollalis
Hygiene 2025, 5(4), 44; https://doi.org/10.3390/hygiene5040044 - 1 Oct 2025
Abstract
Artificial intelligence (AI) is progressively being utilized in the healthcare sector to enhance efficiency, alleviate administrative burdens, and improve patient care outcomes. In the secondary healthcare sector, AI presents a range of opportunities as well as challenges. This study investigates the viewpoints of
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Artificial intelligence (AI) is progressively being utilized in the healthcare sector to enhance efficiency, alleviate administrative burdens, and improve patient care outcomes. In the secondary healthcare sector, AI presents a range of opportunities as well as challenges. This study investigates the viewpoints of healthcare professionals regarding the adoption of AI in Greece, emphasizing the anticipated advantages and apprehensions associated with its integration. A cross-sectional descriptive study was carried out to collect responses from healthcare professionals at the General Hospital of “Evangelismos”, which is the largest hospital in Athens, Greece. A questionnaire was utilized and distributed over a period of four months, involving 513 registered healthcare professionals (comprising 136 physicians, 235 nursing staff, and 142 other healthcare personnel). Each participant had a minimum of one year of clinical experience and was selected using a convenience sampling method. The questionnaire comprised two parts: one focused on evaluating the AI knowledge and attitudes of healthcare professionals, and the other collected demographic data. The overall comprehension of knowledge pertaining to AI among healthcare professionals was evaluated as moderate, resulting in a mean score of 3.39. A distinction exists among different personnel categories, with physicians (M = 3.73) demonstrating a greater understanding of AI and a firm conviction that AI cannot supplant human positions. Conversely, nursing personnel appear to express apprehension regarding the implications of AI on the human experience, with a notable concern about potential replacement and job loss (M = 2.63), which was identified as the lowest-ranked issue. This latter concern is also echoed by other healthcare personnel (M = 2.90). Nevertheless, the majority of participants regard the prospective use of AI favorably, demonstrate confidence in its application, and contend that the benefits outweigh the possible risks. Sufficient training and ongoing updates would enhance employees’ comprehension of AI and their awareness of its potential benefits within the healthcare sector.
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(This article belongs to the Section Health Promotion, Social and Behavioral Determinants)
Open AccessArticle
Exploration of Psychosocial Factors in Peruvian Workers: A Quantitative Analysis of Qualitative Categorizations
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Arturo Juárez-García, César Merino-Soto and Javier García-Rivas
Hygiene 2025, 5(4), 43; https://doi.org/10.3390/hygiene5040043 - 30 Sep 2025
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This study aimed to explore psychosocial factors in a sample of Peruvian workers, examine their convergence with the PROPSIT model, and identify the emergence of new or idiosyncratic psychosocial dimensions. At the same time, the quality and efficiency of the categorization process were
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This study aimed to explore psychosocial factors in a sample of Peruvian workers, examine their convergence with the PROPSIT model, and identify the emergence of new or idiosyncratic psychosocial dimensions. At the same time, the quality and efficiency of the categorization process were evaluated. n = 48 workers were contacted by a non-probabilistic sampling method and asked to fill out a form with open-ended questions that explored negative stressors and positive engaging factors. Some strategies were used to assess the quality and efficiency of the categorization process. The results showed that the quality, speed, and reliability of the categorization procedure were satisfactory, and several categories were aligned with the PROPSIT model and other literature, both in their negative aspects (workload and rhythm, working hours, shifts, etc.) and positive aspects (rewarding tasks, atmosphere of unity, etc.). The emerging new categories were confined to aspects of teamwork and conflict climate, as well as topics such as order, cleanliness, and recreation. These findings underline the need to adapt existing models and instruments to capture idiosyncratic aspects of the Peruvian work environment. In conclusion, this study validated an efficient mixed approach for categorizing psychosocial work factors in Peru, revealing both PROPSIT-aligned and novel context-specific categories, and highlighting the need for culturally adapted tools and broader validation.
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Open AccessArticle
Assessment of Bacterial Presence Among New and “In Use” Resealable Biomaterials Within the Pediatric Dental Clinic
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Gavin Banning, Cindy Kim, Carter Wilkerson, Shelley J. Williams, Karl Kingsley and Victoria Sullivan
Hygiene 2025, 5(3), 42; https://doi.org/10.3390/hygiene5030042 - 9 Sep 2025
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Background: Dental offices and clinics utilize a variety of dental materials that are delivered in reusable containers and dispensers. However, many of these materials, including NeoPutty, BC Putty, Flowable, and Diapex, may be subject to bacterial contamination and microbial exposures from the surrounding
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Background: Dental offices and clinics utilize a variety of dental materials that are delivered in reusable containers and dispensers. However, many of these materials, including NeoPutty, BC Putty, Flowable, and Diapex, may be subject to bacterial contamination and microbial exposures from the surrounding dental office environment. Objectives: The aim of this study was to quantify and identify microbial contamination, specifically in regard to these reusable dental materials. Methods: Surfaces of new and used reusable and resealable tubes where the material dispenses and the interior surfaces of the cap were swabbed and cultured. DNA was isolated from each sample and quantitative polymerase chain reaction (qPCR) was performed to determine the presence or absence of microbial contamination, as well as the relative abundance. Results: Microbial contamination was observed among all of the “in use” samples from both the dispensing end and the interior surfaces of the cap and was strongly associated with the amount of usage. Conclusions: These data suggest that environmental contamination may be present in measurable and quantifiable amounts on reusable and resealable dental materials, which suggest the need to create protocols for sanitizing the surfaces of reusable materials to reduce the presence of microbial contamination identified in similar clinical settings.
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Open AccessArticle
In Vitro Evaluation of the Effectiveness of a Commercial Enzymatic Product Against Pseudomonas aeruginosa Biofilms According to the Parameters of Use
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Caroline Le Sénéchal, Yoann Fautras, Caroline Tokarski and Sébastien Vilain
Hygiene 2025, 5(3), 41; https://doi.org/10.3390/hygiene5030041 - 4 Sep 2025
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Biofilms are the source of numerous issues in the food, pharmaceutical, and production industries, making their control a major component of economic and public health. Among anti-biofilm strategies, enzyme-based products that target the biofilm matrix have proven effectiveness against multiple bacterial species. We
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Biofilms are the source of numerous issues in the food, pharmaceutical, and production industries, making their control a major component of economic and public health. Among anti-biofilm strategies, enzyme-based products that target the biofilm matrix have proven effectiveness against multiple bacterial species. We tested the efficacy of a commercial product, Baso Bionil SL40® (SL40; Stockmeier France, Saint-Jacques de la lande, France), against biofilms of Pseudomonas aeruginosa under various conditions of temperature, concentration, pH, and incubation time. SL40 contains two enzymes: a subtilisin protease and an α-amylase glycosidase. Our results showed that SL40 removed up to 85% of the biofilm biomass compared to tris solutions. SL40’s efficacy was strongly influenced by the presence of the enzymes and both temperature and concentration. Enzymatic activity was maintained from 20 °C to 60 °C and at pH values ranging from 5 to 9, with effective concentrations corresponding to SL40 dilutions from 3/200 to 1/200 in 50 mM tris solutions. Additionally, we observed that the P. aeruginosa biofilm biomass after pH 9 tris solution treatment was twice compared to a H2O washing. Our results confirm the potential of enzymes against biofilms, highlight the need to define optimal application conditions, and support their integration into combined strategies for complete biofilm removal.
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Open AccessArticle
Evaluation of Prepacked Bone Cement Mixing Systems in Arthroplasty: Implications for Intraoperative Hygiene and Contamination Risk
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Christian Paul, Pablo Sanz Ruiz, Muhamed Zeneli and Klaus-Dieter Kühn
Hygiene 2025, 5(3), 40; https://doi.org/10.3390/hygiene5030040 - 4 Sep 2025
Abstract
In cemented endoprosthetics, closed prepacked mixing systems represent the most advanced generation of cementing technology. (1) Background: The purpose of the present study is to evaluate four approved prepacked systems—Palacos® R+G pro, SmartMix™ Cemvac GHV, Optipac® Refobacin and Cemex® System
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In cemented endoprosthetics, closed prepacked mixing systems represent the most advanced generation of cementing technology. (1) Background: The purpose of the present study is to evaluate four approved prepacked systems—Palacos® R+G pro, SmartMix™ Cemvac GHV, Optipac® Refobacin and Cemex® System Genta—with a focus on practical handling and intraoperative hygiene. (2) Method: The systems were evaluated according to established standard test methods for bone cements (ISO 5833), including dough time, setting time, additional mechanical tests and the level of system closure. (3) Results: The results show that all systems are safe to use and meet the general requirements, but there are relevant differences in terms of intraoperative hygiene. The Palacos R+G pro system shows significantly shorter doughing and setting times, which helps to minimize wound exposure during surgery and thus significantly reduces the overall operating time and the risk of bacterial contamination. Two of the systems cannot be classified as completely closed “pre-packaged systems.” In two cases, the system must be temporarily opened before mixing to insert the mixing element, which may result in a temporary but clinically relevant impairment of sterility and a corresponding potential risk of contamination. (4) Conclusion: From a hygienic point of view, systems that remain completely closed throughout the entire preparation process can offer advantages in terms of infection prevention. This was the case for all systems tested. Short handling times, reduced exposure of the surgical site and a shorter overall duration of the procedure could further improve intraoperative safety and reduce the risk of contamination. In terms of intraoperative hygiene, the Palacos R+G pro system achieved the best results compared to the three other systems tested due to its rapid readiness for use and comparatively short setting time (according to ISO 5833). Cemex System Genta performed worst in this respect due to its late doughing time and setting time.
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(This article belongs to the Section Hygiene in Healthcare Facilities)
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Open AccessArticle
Alcohol-Based Hand Rub Purchase as a Surrogate Marker for Monitoring Hand Hygiene in Nursing Homes: Results from a French Regional Survey over the 2018–2023 Period
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Louis Alglave, Manon Caudron, Karine Faure, Charlotte Moreau and Catherine J. Mullié
Hygiene 2025, 5(3), 39; https://doi.org/10.3390/hygiene5030039 - 4 Sep 2025
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Hand hygiene (HH) is universally recognized as a pillar of infection prevention and control (IPC), both in hospitals and long-term care facilities such as nursing homes (NHs). An annual national survey based on the voluntary notification of purchased alcohol-based hand rubs (ABHRs) has
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Hand hygiene (HH) is universally recognized as a pillar of infection prevention and control (IPC), both in hospitals and long-term care facilities such as nursing homes (NHs). An annual national survey based on the voluntary notification of purchased alcohol-based hand rubs (ABHRs) has been set up in France since 2019 to appraise the compliance to HH in NHs. The results pertaining to the Hauts-de-France (HdF) region were analyzed over the 2018–2023 period. The impact of factors such as the year, structure status, availability of an infection control link (ICL) nurse and/or an IPC team as well as constant participation in the survey was evaluated. A clear rise in the consumption of ABHRs was witnessed in 2020 followed by a progressive return to the 2018 baseline by 2023 for most NHs. This decrease was significantly less marked in NHs constantly participating in the survey. The ABHR indicator’s annual values were significantly higher in public NHs than in associative ones and in NHs with available ICL nurses/IPC teams. Finally, even though less than 50% of regional NHs reached the target of four daily ABHR uses per resident, they more frequently met it than French ones as a whole. This result underscores the need for ceaselessly reiterating the importance of HH and for pursuing training efforts in NHs with the help of ICL nurses and IPC teams.
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Open AccessSystematic Review
Efficacy and Safety of Natural Versus Conventional Toothpastes and Mouthwashes in Gingivitis Management: A Systematic Review
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Angelo Michele Inchingolo, Grazia Marinelli, Valeria Colonna, Benito Francesco Pio Pennacchio, Roberto Vito Giorgio, Francesco Inchingolo, Daniela Di Venere, Andrea Palermo, Giuseppe Minervini, Alessio Danilo Inchingolo and Gianna Dipalma
Hygiene 2025, 5(3), 38; https://doi.org/10.3390/hygiene5030038 - 4 Sep 2025
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Gingivitis is a common and reversible inflammatory condition caused by dental plaque accumulation, which, if left untreated, can progress to periodontitis. Conventional oral care products like chlorhexidine (CHX) and fluoride are effective in plaque control but are often associated with adverse effects such
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Gingivitis is a common and reversible inflammatory condition caused by dental plaque accumulation, which, if left untreated, can progress to periodontitis. Conventional oral care products like chlorhexidine (CHX) and fluoride are effective in plaque control but are often associated with adverse effects such as dental staining and mucosal irritation. This systematic review aimed to compare the efficacy and safety of natural versus conventional toothpastes and mouthwashes in managing plaque-induced gingivitis. The review followed PRISMA guidelines and was registered in PROSPERO (No. 1008296). A systematic search was conducted across PubMed, Web of Science, and Scopus for English-language clinical studies published between 2015 and 2025. Eligible studies included randomized controlled trials and clinical trials on human subjects with plaque-induced gingivitis. Exclusion criteria were studies on animals, in vitro experiments, review articles, and studies lacking control groups. Data extracted included intervention type, sample characteristics, clinical indices (PI, GI, SBI), inflammatory biomarkers, adverse events, and patient adherence. A narrative synthesis was conducted due to study heterogeneity. Fifteen studies were included. Natural products such as neem, green tea, aloe vera, and propolis demonstrated comparable effectiveness to CHX and fluoride in reducing gingival inflammation and plaque indices, with a lower incidence of side effects. In particular, natural formulations showed superior tolerability and better patient compliance, especially in long-term use. However, variability in concentration and the formulation of natural products limits their clinical standardization. In conclusion, natural oral care products appear to be effective and better-tolerated alternatives to conventional agents in managing gingivitis. Nonetheless, further long-term, standardized clinical trials are needed to confirm their efficacy and define optimal formulations.
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(This article belongs to the Special Issue Dental Biofilm Control and Oral Health)
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Open AccessArticle
Eco-Friendly vs. Traditional Cleaning in Healthcare Settings: Microbial Safety and Environmental Footprint
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Riccardo Fontana, Mattia Buratto, Anna Caproni, Chiara Nordi, Mariangela Pappadà, Martina Facchini, Cesare Buffone, Beatrice Bandera, Luciano Vogli and Peggy Marconi
Hygiene 2025, 5(3), 37; https://doi.org/10.3390/hygiene5030037 - 22 Aug 2025
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Growing concern for environmental sustainability has resulted in the implementation of sanitization methods that respect ecological principles. This research evaluates a “green” sanitizing protocol that uses CAM (Minimum Environmental Criteria)-compliant products against a traditional protocol within two ASL Roma 1 facilities. The study
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Growing concern for environmental sustainability has resulted in the implementation of sanitization methods that respect ecological principles. This research evaluates a “green” sanitizing protocol that uses CAM (Minimum Environmental Criteria)-compliant products against a traditional protocol within two ASL Roma 1 facilities. The study performed a Life Cycle Assessment (LCA) following ISO 14040, ISO 14044, and ISO 14067 standards to measure greenhouse gases emissions. Microbiological sampling was conducted according to established protocols across three different risk zones utilizing contact plates and surface swabs. The Life Cycle Assessment showed that CO2 emissions reduced by 49.6% to 53.3% at different sites due to reduced energy use together with concentrated detergents and improved washing cycles. Microbiological testing revealed notable decreases in contamination rates across both cleaning systems yet demonstrated the “green” system achieved superior results specifically within high-risk zones. The “green” protocol matched traditional cleaning methods hygienically but delivered significant environmental advantages which positions it as a sustainable hospital cleaning solution.
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Open AccessArticle
Bacterial Inactivation by Common Food Industry Sanitizers
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Vinícius do Amaral Flores, Angélica Olivier Bernardi, Bruna Lago Tagliapietra, Maximiliano Escalona, Maritiele Naissinger da Silva, Juliana Copetti Fracari, Marina Venturini Copetti and Neila Silvia Pereira dos Santos Richards
Hygiene 2025, 5(3), 36; https://doi.org/10.3390/hygiene5030036 - 19 Aug 2025
Abstract
The efficacy of peracetic acid (0.05%, 0.5%, and 1%), sodium hypochlorite (0.2%, 0.6%, and 1%), and benzalkonium chloride (0.3%, 1.15%, and 2%) was evaluated against Staphylococcus aureus (ATCC 6538), Salmonella enterica serovar Typhimurium, (ATCC 14028), Enterococcus hirae (ATCC 8043), Pseudomonas aeruginosa (ATCC 9027),
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The efficacy of peracetic acid (0.05%, 0.5%, and 1%), sodium hypochlorite (0.2%, 0.6%, and 1%), and benzalkonium chloride (0.3%, 1.15%, and 2%) was evaluated against Staphylococcus aureus (ATCC 6538), Salmonella enterica serovar Typhimurium, (ATCC 14028), Enterococcus hirae (ATCC 8043), Pseudomonas aeruginosa (ATCC 9027), Escherichia coli (ATCC 9027), and Listeria monocytogenes (ATCC 35152) using stainless steel discs, following European Committee for Standardization (CEN) guidelines. According to CEN, a sanitizer must achieve at least a 5 Log10 CFU reduction to be considered effective. Peracetic acid at 1% demonstrated the highest inactivation capacity, reducing all tested strains by more than 7 Log10 CFU/mL. P. aeruginosa (ATCC 9027) showed high tolerance to sodium hypochlorite and benzalkonium chloride, with reductions below 2 Log10 CFU/mL even at maximum concentrations. Both sodium hypochlorite and benzalkonium chloride, at their highest tested concentrations, effectively inactivated S. aureus, S. typhimurium, E. hirae, L. monocytogenes, and E. coli, achieving reductions greater than 7 Log10 CFU/mL. Overall, sanitizers were effective only at intermediate or maximum concentrations recommended by the manufacturers, suggesting that minimum label concentrations should be avoided to ensure microbiological control.
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(This article belongs to the Section Food Hygiene and Safety)
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Open AccessBrief Report
Simulation-Based Education to Improve Hand Hygiene Practices: A Pilot Study in Sub-Saharan Africa
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Paula Rocha, Stephanie Norotiana Andriamiharisoa, Ana Catarina Godinho, Pierana Gabriel Randaoharison, Lugie Harimalala, Lova Narindra Randriamanantsoa, Oni Zo Andriamalala, Emmanuel Guy Raoelison, Jane Rogathi, Paulo Kidayi, Christina Mtuya, Rose Laisser, Eyeshope J. Dausen, Pascalina Nzelu, Barbara Czech-Szczapa, Edyta Cudak-Kasprzak, Marlena Szewczyczak, João Graveto, Pedro Parreira, Sofia Ortet and M. Rosário Pintoadd
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Hygiene 2025, 5(3), 35; https://doi.org/10.3390/hygiene5030035 - 16 Aug 2025
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Hand hygiene is a key measure to prevent healthcare-associated infections (HAIs), yet compliance remains low in Sub-Saharan Africa (SSA), due to limited resources, insufficient training, and behavioral challenges. Simulation-based education offers a promising approach to enhance technical and non-technical skills in safe learning
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Hand hygiene is a key measure to prevent healthcare-associated infections (HAIs), yet compliance remains low in Sub-Saharan Africa (SSA), due to limited resources, insufficient training, and behavioral challenges. Simulation-based education offers a promising approach to enhance technical and non-technical skills in safe learning environments, promoting behavioral change and patient safety. This study aimed to develop and pilot a contextually adapted hand hygiene simulation-based learning scenario for nursing students in SSA. Grounded in the Medical Research Council (MRC) Framework and Design-Based Research principles, a multidisciplinary team from European and African higher education institutions (HEIs) co-created this scenario, integrating international and regional hand hygiene guidelines. Two iterative pilot cycles were conducted with expert panels, educators, and students. Data from structured observation and post-simulation questionnaires were analyzed using descriptive statistics. The results confirm the scenario’s feasibility, relevance, and educational value. The participants rated highly the clarity of learning objectives (M = 5.0, SD = 0.0) and preparatory materials (M = 4.6, SD = 0.548), reporting increased knowledge/skills and confidence and emphasizing the importance of clear roles, structured facilitation, and real-time feedback. These findings suggest that integrating simulation in health curricula could strengthen HAI prevention and control in SSA. Further research is needed to evaluate long-term outcomes and the potential for wider implementation.
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Open AccessArticle
Respiratory Infections in Adults and Inequality: An Analysis of Deaths and Their Socioeconomic Determinants in Brazil
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Nikolas Lisboa Coda Dias, Pedro Henrique Santos Serafim Ferraz, Rayssa Lopes de Souza, Mariana Felix Maccari, Manoel Reverendo Vidal, Wallisen Tadashi Hattori and Stefan Vilges de Oliveira
Hygiene 2025, 5(3), 34; https://doi.org/10.3390/hygiene5030034 - 13 Aug 2025
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Introduction: Respiratory infections cause serious complications responsible for a significant number of deaths in Brazil. In addition, the causes of death can be influenced by social and economic inequalities in Brazilian regions. Objective: To analyze the epidemiological profile and the influence of demographic
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Introduction: Respiratory infections cause serious complications responsible for a significant number of deaths in Brazil. In addition, the causes of death can be influenced by social and economic inequalities in Brazilian regions. Objective: To analyze the epidemiological profile and the influence of demographic and socioeconomic factors on deaths from respiratory infections in the adult population between 2014 and 2023 in Brazil. Methods: This was an analytical ecological study using data from the Death Information System. Death incidences were calculated. Multinomial logistic regressions and correlation tests were used to analyze the influence of socioeconomic factors on deaths. Results: There were high incidences of deaths from unspecified pneumonia, unconfirmed tuberculosis and complicated influenza. Deaths from pneumonia and the Gini index were positively correlated, considering the variables black ethnicity (R = 0.894), age over 90 (R = 0.869) and no schooling (R = 0.818) before the pandemic. The odds ratio of death from tuberculosis and influenza in the 70–79 age group (OR = 3.97) and black ethnicity (OR = 1.24), respectively, were higher in the pandemic and post-pandemic periods compared to the previous period. Conclusions: Deaths from respiratory infections were mainly influenced by demographic variables and socioeconomic inequalities in Brazil.
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Open AccessReview
A Tiered Vaccine Framework: Prioritizing Tier 1 Vaccines to Restore Public Confidence
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Konstantin Gus Kousoulas, Ojasvi Dutta, Harikrishnan Mohan and Agustin Fernandez Santana
Hygiene 2025, 5(3), 33; https://doi.org/10.3390/hygiene5030033 - 8 Aug 2025
Abstract
The term “vaccine” has been broadly and inconsistently applied to a range of products with widely divergent immunological outcomes, leading to the erosion of public trust and confusion among both medical professionals and the public. Historically, and by broad public understanding, a vaccine
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The term “vaccine” has been broadly and inconsistently applied to a range of products with widely divergent immunological outcomes, leading to the erosion of public trust and confusion among both medical professionals and the public. Historically, and by broad public understanding, a vaccine is expected to prevent infection, transmission, and disease through the induction of sterilizing, or true neutralizing immunity, specifically, the prevention of pathogen entry and replication in vivo. This ideal extends beyond the mere elicitation of neutralizing antibodies demonstrable in vitro. This paper proposes a three-tier classification system designed to differentiate products currently designated as “vaccines,” specifically to address the distinction between those that meet the traditional, highest-expectation definition (Tier 1), therapeutic vaccines that primarily prevent disease (Tier 2), and immunomodulatory therapeutics that primarily reduce disease severity (Tier 3). By detailing the mechanism of action of each product and emphasizing the urgent need for this refined classification, our aim is to restore public confidence in vaccination programs, improve understanding of vaccine-induced immunity among healthcare professionals, and empower informed decision-making by the public. We argue that a clearer understanding of vaccine capabilities will ultimately lead to increased vaccine uptake for those vaccines that do prevent infection, transmission, and disease.
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(This article belongs to the Section Public Health and Preventive Medicine)
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Open AccessArticle
In Vitro Biofilm Formation Kinetics of Pseudomonas aeruginosa and Escherichia coli on Medical-Grade Polyether Ether Ketone (PEEK) and Polyamide 12 (PA12) Polymers
by
Susana Carbajal-Ocaña, Kristeel Ximena Franco-Gómez, Valeria Atehortúa-Benítez, Daniela Mendoza-Lozano, Luis Vicente Prado-Cervantes, Luis J. Melgoza-Ramírez, Miguel Delgado-Rodríguez, Mariana E. Elizondo-García and Jorge Membrillo-Hernández
Hygiene 2025, 5(3), 32; https://doi.org/10.3390/hygiene5030032 - 1 Aug 2025
Cited by 1
Abstract
Biofilms, structured communities of microorganisms encased in an extracellular matrix, are a major cause of persistent infections, particularly when formed on medical devices. This study investigated the kinetics of biofilm formation by Escherichia coli and Pseudomonas aeruginosa, two clinically significant pathogens, on
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Biofilms, structured communities of microorganisms encased in an extracellular matrix, are a major cause of persistent infections, particularly when formed on medical devices. This study investigated the kinetics of biofilm formation by Escherichia coli and Pseudomonas aeruginosa, two clinically significant pathogens, on two medical-grade polymers: polyether ether ketone (PEEK) and polyamide 12 (PA12). Using a modified crystal violet staining method and spectrophotometric quantification, we evaluated biofilm development over time on polymer granules and catheter segments composed of these materials. Results revealed that PEEK surfaces supported significantly more biofilm formation than PA12, with peak accumulation observed at 24 h for both pathogens. Conversely, PA12 demonstrated reduced bacterial adhesion and lower biofilm biomass, suggesting surface characteristics less conducive to microbial colonization. Additionally, the study validated a reproducible protocol for assessing biofilm formation, providing a foundation for evaluating anti-biofilm strategies. While the assays were performed under static in vitro conditions, the findings highlight the importance of material selection and early prevention strategies in the design of infection-resistant medical devices. This work contributes to the understanding of how surface properties affect microbial adhesion and underscores the critical need for innovative surface modifications or coatings to mitigate biofilm-related healthcare risks.
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(This article belongs to the Section Hygiene in Healthcare Facilities)
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Open AccessArticle
The Relationship Between Maternal Employment and Educational Status and Children’s Oral Health: A Study Focusing on the Panel Study on Korean Children
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Eun-Jeong Kim, Su-Min Kang, Min-Jeong Ko, Da-Yeon Kim, Je-Yeong Kim, Mi-Seong Choi and Hye-ju Lee
Hygiene 2025, 5(3), 31; https://doi.org/10.3390/hygiene5030031 - 15 Jul 2025
Cited by 1
Abstract
Parental attention and care is essential for children and adolescents who are unable to take care of their own oral health. There have been studies on the characteristics of mothers and the oral conditions of children in Korea, but there are very few
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Parental attention and care is essential for children and adolescents who are unable to take care of their own oral health. There have been studies on the characteristics of mothers and the oral conditions of children in Korea, but there are very few previous studies that report on the oral health status of children according to the employment status of mothers. The aim of this study was to investigate the relationship between maternal employment and educational status and children’s oral health. Using data from the 10th Panel Study on Korean Children (PSKC), we analyzed the association between maternal employment and education status and the occurrence of dental caries among 1175 nine-year-old Korean children. The relationship was examined through cross-tabulation and logistic regression analysis. After adjusting for the mother’s age, parental style, parental relationship, family talk time, family meal time, leisure time, area of residence, and household income, the study found that children with working and studying mothers were 1.159 times more likely to have dental caries than children with non-working and non-studying mothers. The relationship between maternal employment and educational status and children’s oral health was confirmed. Based on the results of this study, it is expected that systematic follow-up studies will be needed to better understand the association and causal relationship between dental caries and oral disease in children according to whether mothers are employed or educated.
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Open AccessArticle
Assessment of the Immune Response to Coxiella burnetii in Rural Areas of the Thessaly Region Following the Daniel Floods
by
Magdalini Christodoulou, Ourania S. Kotsiou, Konstantinos Tsaras, Charalambos Billinis, Konstantinos I. Gourgoulianis and Dimitrios Papagiannis
Hygiene 2025, 5(3), 30; https://doi.org/10.3390/hygiene5030030 - 13 Jul 2025
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Background: In September 2023, Storm Daniel triggered catastrophic flooding across Thessaly, in central Greece, leading to the deaths of approximately 483,476 animals and heightening concerns about zoonotic diseases, particularly Q fever caused by Coxiella burnetii. Sofades, a municipality in the Karditsa
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Background: In September 2023, Storm Daniel triggered catastrophic flooding across Thessaly, in central Greece, leading to the deaths of approximately 483,476 animals and heightening concerns about zoonotic diseases, particularly Q fever caused by Coxiella burnetii. Sofades, a municipality in the Karditsa region that is severely impacted by the floods, emerged as a critical area for evaluating the risk of zoonotic disease transmission. This study aimed to determine the seroprevalence status of Coxiella burnetii Phase 1 IgA antibodies among residents in the rural area of Sofades after the Daniel floods. Methods: Serum samples were obtained from a convenient sample of residents with livestock exposure between 1 March and 31 March 2024. Enzyme-linked immunosorbent assay (ELISA) was used to detect Coxiella burnetii Phase 1 IgA antibodies. Descriptive analyses summarized demographic data, and logistic regression was employed to examine the association between gender, age, and positive ELISA results. Results: The overall seroprevalence was 16.66%. Males had a significantly higher positivity rate (28.57%) than females (6.25%). Seropositivity was more frequent among individuals aged 41–80 years, with peak prevalence observed in the 61–80 age group. Conclusions: This cross-sectional study offers a snapshot of Coxiella burnetii exposure in a high-risk rural population post-flood. The slightly higher seroprevalence in Sofades (16.66%) compared to Karditsa (16.1%) suggests limited influence of environmental factors on transmission. Despite limitations in causal inference, the findings highlight the need for enhanced surveillance and targeted public health measures. Longitudinal studies are needed to assess the long-term impact of environmental disasters on Q fever dynamics.
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Open AccessArticle
Hand Hygiene in Greek Public Hospitals: Exploring Knowledge, Self-Reported Compliance, and the Impact of a Behavioral Economics-Based Nudge
by
Angeliki Flokou, Styliani Spyrou, Dimitris A. Niakas and Vassilis Aletras
Hygiene 2025, 5(3), 29; https://doi.org/10.3390/hygiene5030029 - 9 Jul 2025
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Healthcare-associated infections (HAIs), also referred to as nosocomial or hospital-acquired infections, are a significant cause of death worldwide, with hand hygiene being the most powerful means to tackle them. The present study had a twofold aim: first, to assess the level of knowledge
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Healthcare-associated infections (HAIs), also referred to as nosocomial or hospital-acquired infections, are a significant cause of death worldwide, with hand hygiene being the most powerful means to tackle them. The present study had a twofold aim: first, to assess the level of knowledge and the degree of compliance with hand hygiene practices based on healthcare workers’ self-reports; second, to evaluate whether behavioral economics techniques, specifically a poster combining an image and an informational message, designed according to the published literature, can increase the level of healthcare workers’ compliance with hand hygiene. Factors that potentially affect compliance were also examined. This study involved distributing a questionnaire to healthcare workers in Greek public hospitals, from which 314 completed responses were collected during the last quarter of 2023. Participants were randomly and evenly assigned to an intervention group that received a nudging poster or to a control group, which did not. Self-reported compliance with hand hygiene practices was high, based on two latent variables derived through exploratory factor analysis, although knowledge of germ transmission was moderate, as reflected in the relevant knowledge question scores. However, nudging had no statistically significant effect on hand hygiene behavior. Compliance was associated with several factors, including gender, age, work experience, profession, perceived adequacy of available hygiene-related resources, and perceived consequences of nosocomial infections on patient outcomes.
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Open AccessArticle
Evaluation of Water Safety Plan Compliance in Italian Hospitals According to Legislative Decree 18/23 and Directive EU 2020/2184: A Cross-Sectional Study
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Maria Teresa Montagna, Matteo Moro, Beatrice Casini, Ida Iolanda Mura, Gianfranco Finzi, Valentina Spagnuolo, Antonella Francesca Savino, Fabrizio Fasano, Francesco Triggiano, Lucia Bonadonna and Osvalda De Giglio
Hygiene 2025, 5(3), 28; https://doi.org/10.3390/hygiene5030028 - 2 Jul 2025
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In 2020, Directive (EU) 2020/2184 was published and subsequently transposed into Italian legislation via Legislative Decree 18/23 (Lgs.D. 18/23). The Directive aims to protect public health through a proactive approach based on a site-specific risk analysis along the entire water supply chain (Water
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In 2020, Directive (EU) 2020/2184 was published and subsequently transposed into Italian legislation via Legislative Decree 18/23 (Lgs.D. 18/23). The Directive aims to protect public health through a proactive approach based on a site-specific risk analysis along the entire water supply chain (Water Safety Plan, WSP). Between February and November 2024, a survey was conducted in Italy to assess both hospitals’ knowledge of Lgs.D. 18/23 and the application of the WSP in these facilities. A total of 300 hospitals were asked to complete an anonymous questionnaire containing 60 questions about the characteristics of the facility and the management of the water network, including the chemical–physical and microbiological monitoring of Legionella and other microbiological parameters. A total of 102 questionnaires were sent out (response rate: 34%), but only 72 were properly completed and analyzed. The results of the chemical–physical monitoring are not presented in this manuscript. Overall, 52.8% of the hospitals were built before 2000, and most are aware of Directive (EU) 2020/2184, Lgs.D.18/23 (80.6%), in particular, Article 9 on the risk assessment and management of internal water systems and the guidelines for its implementation (77.8%). All hospitals perform annual microbiological water testing, including Legionella analysis, with a detection rate of <50%. National guidelines for the implementation of WSPs are known in 75% of the hospitals, but only 38.9% have started planning to implement them, and 13.9% organize staff training on the subject. The questionnaire responses highlight the need to train hospital staff in water system risk management and WSP planning, which will be mandatory by 2029.
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