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Natural Products: Source, Function, and Application

A Special Issue of Applied Sciences (ISSN 2076-3417) belonging to the section "Food Science and Technology".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 1019

Editors


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Guest Editor
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, Bulgaria
Interests: extraction; antioxidant activity; natural product chemistry; phytochemicals

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Guest Editor
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 9, 1113 Sofia, Bulgaria
Interests: phytonutrients; biologically active compounds
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Natural products have a long history of use as therapeutic agents in traditional medicine, are part of a broad range of commercial products, and interest in their potential as sources of structurally diverse bioactive compounds for applications in pharmaceuticals, nutraceuticals, cosmetics, and functional foods is increasing.

In recent years, considerable attention has been directed toward the discovery, isolation, and characterization of specialized metabolites with valuable pharmacological properties. Elucidating their composition and activity is important in terms of their therapeutic efficacy, standardization and control, and also generates the possibility of discovering new biologically active compounds.

Advances in extraction techniques, phytochemical analysis, and modern spectroscopic and spectrometric methods have significantly expanded the possibilities of investigating the chemical composition and biological activity of natural products. Particular interest is focused on secondary metabolites exhibiting antioxidant, antimicrobial, anti-inflammatory, and other health-promoting properties.

This Special Issue aims to highlight recent progress in the chemistry and application of natural products obtained from botanical, microbial, marine and animal sources as well as bee-derived products.

Original research articles and reviews addressing, but not limited to, the extraction, isolation, structural characterization, phytochemical profiling, and biological activity of natural compounds, as well as their potential applications in health-related fields, are particularly welcome.

Dr. Ralitsa Chimshirova
Dr. Boryana S. Trusheva
Guest Editors

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Keywords

  • natural products
  • bioactive compounds
  • phytochemical analysis
  • extraction and isolation
  • structure elucidation
  • biological activity
  • health-related applications

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Published Papers (2 papers)

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Research

16 pages, 939 KB  
Article
Rosa rugosa Pomace as a Functional Ingredient: Bioactive Potential and Its Application in Gummy Gel Systems
by Gabriela Kowalska, Kamila Kulbat-Warycha and Aleksandra Bilczewska
Appl. Sci. 2026, 16(14), 7185; https://doi.org/10.3390/app16147185 - 17 Jul 2026
Viewed by 318
Abstract
This study investigated the valorisation potential of dried and frozen pomace obtained as by-products from the processing of Rosa rugosa fruits. The antioxidant activity of the pomace was assessed using electron paramagnetic resonance (EPR) spectroscopy with the TEMPO radical. Total phenolic content was [...] Read more.
This study investigated the valorisation potential of dried and frozen pomace obtained as by-products from the processing of Rosa rugosa fruits. The antioxidant activity of the pomace was assessed using electron paramagnetic resonance (EPR) spectroscopy with the TEMPO radical. Total phenolic content was determined using the Folin–Ciocalteu method, and individual phenolic compounds were analysed by UHPLC. Frozen pomace exhibited significantly higher antioxidant activity than dried pomace (2.76 vs. 1.64 mmol TE/100 g d.m.) and contained greater amounts of ellagic acid (19.29 vs. 13.08 mg/100 g d.m.), gallic acid (17.05 vs. 5.07 mg/100 g d.m.), procyanidin B2 (14.92 vs. 11.88 mg/100 g d.m.), and catechin (2.84 vs. 1.12 mg/100 g d.m.). To assess technological applicability, Rosa rugosa pomace was incorporated into an agar-based model fruit gel system at levels of 3%, 5%, and 10% (w/w). The enriched gels were evaluated in terms of antioxidant activity, total phenolic content, and physicochemical parameters, including colour, water activity, and moisture content. The incorporation of pomace into gel systems was found to significantly increase the total phenolic content, raising it from 48.64 mg GAE/100 g in the control sample to 267.56 mg GAE/100 g in gels containing 10% pomace. Antioxidant activity also increased, rising from 335.79 to 1264.37 μmol TE/100 g. The addition of pomace was also found to reduce the moisture content from 71.02% to 64.17%, while the water activity remained stable (0.821–0.840). Sensory evaluation indicated that gels containing 3–5% pomace achieved the highest consumer acceptance. These results demonstrate that Rosa rugosa pomace is a valuable and sustainable source of functional ingredients that can enhance the bioactive properties of food products while maintaining desirable physicochemical characteristics. Full article
(This article belongs to the Special Issue Natural Products: Source, Function, and Application)
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21 pages, 1787 KB  
Article
Natural Deep Eutectic Solvents as Alternative Media for the Extraction of Phenolic Compounds from Crataegus monogyna
by Hristo Petkov, Vanya Gerasimova, Boryana Trusheva, Zhanina Petkova, Vassya Bankova and Milena Popova
Appl. Sci. 2026, 16(12), 5924; https://doi.org/10.3390/app16125924 - 11 Jun 2026
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Abstract
Natural deep eutectic solvents (NADESs) coupled with ultrasound-assisted extraction (UAE) were evaluated as an extraction technique for phenolic compounds from Crataegus monogyna leaves and flowers. Nine well-known hydrophilic NADESs were investigated as green extraction media, and their extractability was assessed in terms of [...] Read more.
Natural deep eutectic solvents (NADESs) coupled with ultrasound-assisted extraction (UAE) were evaluated as an extraction technique for phenolic compounds from Crataegus monogyna leaves and flowers. Nine well-known hydrophilic NADESs were investigated as green extraction media, and their extractability was assessed in terms of major individual compounds, total flavan-3-ols and proanthocyanidins, as well as antioxidant activity. Water and ethanol–water solutions (70% and 50%, v/v) were used as reference solvents. An HPLC method was developed and partially validated for the quantitative determination of key individual components, including chlorogenic acid, hyperoside, vitexin, vitexin-2″-O-rhamnoside, and vitexin 2″-O-(4‴-O-acetyl)-rhamnoside. The subsequent chemometric analysis of the datasets revealed that the NADES systems choline chloride:urea:water (1:1:6) and choline chloride:glucose:water (5:2:25) exhibited pronounced extraction performance for all investigated metabolites, while preserving high antioxidant activity of the extracts. Pearson correlation coefficients and corresponding p-values demonstrated strong and statistically significant relationships among the majority of the investigated parameters: solvents’ physicochemical properties, the yield of phenolic compounds, and the antioxidant activity of the hawthorn extracts. The results highlight the potential of choline chloride based NADESs containing urea or glucose as alternative solvents for the green production of hawthorn-derived ingredients for functional foods, nutraceuticals, and herbal preparations, thereby contributing to the development of scalable, application-oriented extraction technologies. Full article
(This article belongs to the Special Issue Natural Products: Source, Function, and Application)
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