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BioTech, Volume 13, Issue 4

2024 December - 20 articles

Cover Story: Microbial proteins offer a promising alternative to traditional protein sources. This study examines the effectiveness of a bubble column bioreactor for producing proteins using Klebsiella oxytoca M5A1. This nitrogen-fixing bacterium shows potential in terms of environmentally friendly protein production. We compared the performance of the bubble column system to static fermentation, monitoring key parameters over 48 hours. The results demonstrate superior growth rates, protein yields, and glucose utilization in the bubble column setup. Our findings highlight the advantages of this bioreactor design for microbial protein production, particularly in maintaining ideal conditions for bacterial growth and product formation. View this paper
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Articles (20)

  • Article
  • Open Access
5 Citations
4,855 Views
14 Pages

The Effects of the Combined Co-Expression of GroEL/ES and Trigger Factor Chaperones on Orthopoxvirus Phospholipase F13 Production in E. coli

  • Iuliia A. Merkuleva,
  • Vladimir N. Nikitin,
  • Tatyana D. Belaya,
  • Egor. A. Mustaev and
  • Dmitriy N. Shcherbakov

23 December 2024

Heterologous protein expression often faces significant challenges, particularly when the target protein has posttranslational modifications, is toxic, or is prone to misfolding. These issues can result in low expression levels, aggregation, or even...

(This article belongs to the Section Medical Biotechnology)
  • Article
  • Open Access
5 Citations
2,424 Views
15 Pages

19 December 2024

An eight-week trial was designed to study the effects of arginine (Arg) supplemented diets on the growth, immunity, antioxidant activity, and oxygen-carrying capacity of juvenile Gibel carp (Carassius auratus gibelio). A total of 300 fish (27.53 &plu...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Communication
  • Open Access
2 Citations
2,483 Views
7 Pages

7 December 2024

Sorghum distillers grains (SDGs) produced from a sorghum liquor company were used for generating biohydrogen via dark fermentation at pH 4.5–6.5 and 55 °C with a batch test, and the biohydrogen electricity generation potential was evaluated...

  • Article
  • Open Access
10 Citations
4,917 Views
15 Pages

Optimized Extraction of Polyphenols from Kiwifruit Peels and Their Biological Activities

  • Batoul Shkeir,
  • Nada El Darra,
  • Bilal Azakir,
  • Salma Khazaal and ,
  • Elie Salem Sokhn,
  • Mohamed Koubaa,
  • Richard G. Maroun,
  • Nicolas Louka and
  • Espérance Debs
  • + 4 authors+ 0 author

2 December 2024

(1) Background: Kiwifruit is a globally valued fruit. Its industrial processing produces a substantial amount of waste, particularly peels, which present an appealing potential source of bioactive compounds. This study focuses on optimizing the extra...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Review
  • Open Access
3 Citations
6,403 Views
19 Pages

Prospects in the Use of Cannabis sativa Extracts in Nanoemulsions

  • Ian Vitola,
  • Carlos Angulo,
  • Raul C. Baptista-Rosas,
  • Luis Miguel Anaya-Esparza and ,
  • Zazil Yadel Escalante-García,
  • Angélica Villarruel-López and
  • Jorge Manuel Silva-Jara
  • + 2 authors+ 0 author

2 December 2024

Cannabis sativa plants have been widely investigated for their specific compounds with medicinal properties. These bioactive compounds exert preventive and curative effects on non-communicable and infectious diseases. However, C. sativa extracts have...

(This article belongs to the Special Issue Natural Antioxidants: Determination in Food and Nutraceuticals and Implications on Human Health)
  • Communication
  • Open Access
4 Citations
3,518 Views
11 Pages

Composition, Seasonal Dynamics and Metabolic Potential of the Rhizosphere Microbiome Associated with Wild White Poplar

  • Mikhail I. Popchenko,
  • Dmitry S. Karpov,
  • Natalya S. Gladysh,
  • Maxim A. Kovalev and ,
  • Vsevolod V. Volodin,
  • George S. Krasnov,
  • Alina S. Bogdanova,
  • Nadezhda L. Bolsheva,
  • Maria S. Fedorova and
  • Anna V. Kudryavtseva
  • + 5 authors+ 0 author

1 December 2024

The white poplar (Populus alba) is a dioecious woody plant with significant potential for the phytoremediation of soils. To realize this potential, it is necessary to utilize growth-promoting microorganisms. One potential source of such beneficial mi...

  • Review
  • Open Access
12 Citations
4,940 Views
12 Pages

The Biotechnological Potential of Crickets as a Sustainable Protein Source for Fishmeal Replacement in Aquafeed

  • Aldo Fraijo-Valenzuela,
  • Joe Luis Arias-Moscoso,
  • Oscar Daniel García-Pérez,
  • Libia Zulema Rodriguez-Anaya and
  • Jose Reyes Gonzalez-Galaviz

21 November 2024

As aquaculture production grows, so does the demand for quality and cost-effective protein sources. The cost of fishmeal (FM) has increased over the years, leading to increased production costs for formulated aquafeed. Soybean meal (SBM) is commonly...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
2 Citations
2,307 Views
14 Pages

Leveraging Walnut Somatic Embryos as a Biomanufacturing Platform for Recombinant Proteins and Metabolites

  • Paulo A. Zaini,
  • Katherine R. Haddad,
  • Noah G. Feinberg,
  • Yakir Ophir and ,
  • Somen Nandi,
  • Karen A. McDonald and
  • Abhaya M. Dandekar
  • + 2 authors+ 0 author

15 November 2024

Biomanufacturing enables novel sources of compounds with constant demand, such as food coloring and preservatives, as well as new compounds with peak demand, such as diagnostics and vaccines. The COVID-19 pandemic has highlighted the need for alterna...

  • Review
  • Open Access
10 Citations
4,613 Views
18 Pages

Climate Resilience in Farm Animals: Transcriptomics-Based Alterations in Differentially Expressed Genes and Stress Pathways

  • Chikamagalore Gopalakrishna Shashank,
  • Veerasamy Sejian,
  • Mullakkalparambil Velayudhan Silpa,
  • Chinnasamy Devaraj and ,
  • Aradotlu Parameshwarappa Madhusoodan,
  • Ebenezer Binuni Rebez,
  • Gajendirane Kalaignazhal,
  • Artabandhu Sahoo and
  • Frank Rowland Dunshea
  • + 4 authors+ 0 author

15 November 2024

The livestock sector, essential for maintaining food supply and security, encounters numerous obstacles as a result of climate change. Rising global populations exacerbate competition for natural resources, affecting feed quality and availability, he...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
4 Citations
3,806 Views
16 Pages

14 November 2024

To date, the only probiotic yeast with evidence of health-promoting effects is Saccharomyces cerevisiae var. boulardii. The expanded market including dietary supplements and functional foods supplemented with Saccharomyces cerevisiae var. boular...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Review
  • Open Access
33 Citations
10,912 Views
18 Pages

Honey Bioactive Molecules: There Is a World Beyond the Sugars

  • Gregorio Bonsignore,
  • Simona Martinotti and
  • Elia Ranzato

14 November 2024

Honey’s many bioactive compounds have been utilized historically to cure infectious diseases. Beneficial effects are its antiviral, antibacterial, anti-inflammatory, antioxidant, and immune-stimulating qualities. The bee species, geographic loc...

(This article belongs to the Special Issue Natural Antioxidants: Determination in Food and Nutraceuticals and Implications on Human Health)
  • Article
  • Open Access
2 Citations
3,454 Views
17 Pages

Screening and Characterization of Sialic Acid-Binding Variable Lymphocyte Receptors from Hagfish

  • Mark Rickard N. Angelia,
  • Abigail Joy D. Rodelas-Angelia,
  • Cheolung Yang,
  • Sojeong Park and ,
  • Seung pyo Jeong,
  • Hyeok Jang,
  • Dennis Berbulla Bela-ong,
  • Hobin Jang,
  • Kim D. Thompson and
  • Taesung Jung
  • + 5 authors+ 0 author

12 November 2024

Sialic acid is a diverse group of monosaccharides often found on the termini of N- and O-linked glycans as well as being components of glycoconjugates. Hypersialylation has been associated with the progression of chronic inflammation-mediated disease...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Review
  • Open Access
5,546 Views
16 Pages

11 November 2024

Advances in genome editing technology have made it possible to create genome-edited (GE) animals, which are useful for identifying isolated genes and producing models of human diseases within a short period of time. The production of GE animals mainl...

(This article belongs to the Section Biotechnology Regulation)
  • Article
  • Open Access
3,558 Views
16 Pages

Ultraviolet, Did the Cell See It from the Side or the Bottom? Assessment and Modeling of UV Effects on Cultured Cells Using the CL-1000 UV-Crosslinker

  • Takahiro Oyama,
  • Kai Yanagihara,
  • Anna Arai,
  • Takanori Kamiya and ,
  • Midori Oyama,
  • Takashi Tanikawa,
  • Takehiko Abe and
  • Tomomi Hatanaka
  • + 3 authors+ 0 author

25 October 2024

Numerous natural extracts and compounds have been evaluated for their ability to mitigate the adverse effects of ultraviolet (UV) overexposure. However, variability in the UV doses that trigger biological responses across studies likely arises from i...

  • Article
  • Open Access
3,229 Views
13 Pages

Microbial Protein and Metabolite Profiles of Klebsiella oxytoca M5A1 in a Bubble Column Bioreactor

  • Tawakalt Ayodele,
  • Musiliu Liadi,
  • Abodunrin Tirmidhi Tijani,
  • Kudirat Alarape and ,
  • Christiana Bitrus,
  • Clairmont L. Clementson and
  • Ademola Hammed
  • + 2 authors+ 0 author

19 October 2024

The production of microbial proteins (MPs) has emerged as a critical focus in biotechnology, driven by the need for sustainable and scalable alternatives to traditional protein sources. This study investigates the efficacy of two experimental setups...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
10 Citations
5,133 Views
18 Pages

Phytochemical Composition, Antioxidant, Anti-Helicobacter pylori, and Enzyme Inhibitory Evaluations of Cleistocalyx operculatus Flower Bud and Leaf Fractions

  • Doan Thien Thanh,
  • Mai Thanh Tan,
  • Nguyen Thi My Thu,
  • Pham Nhat Phuong Trinh and ,
  • Pham Thi Hoai Thuong,
  • Pham Thi Giang Tuyet,
  • Luong Thi My Ngan and
  • Tran Trung Hieu
  • + 3 authors+ 0 author

11 October 2024

Six solvent fractions isolated from flower bud and leaf ethanolic extracts of Cleistocalyx operculatus were analyzed for their phytochemical contents, including phenolics, flavonoids, saponins, tannins, and alkaloids. Antioxidant activities were meas...

(This article belongs to the Special Issue Natural Antioxidants: Determination in Food and Nutraceuticals and Implications on Human Health)
  • Article
  • Open Access
2,877 Views
16 Pages

3 October 2024

Cadherin−catenin cell−cell adhesion complexes, composed of cadherin, β-catenin or plakoglobin, and α-catenin (α-cat) molecules, are crucial for maintaining cell−cell contact and are commonly referred to as “ad...

  • Article
  • Open Access
2 Citations
4,888 Views
16 Pages

Structural Analysis and Substrate Specificity of D-Carbamoylase from Pseudomonas

  • Marina Paronyan,
  • Haykanush Koloyan,
  • Hovsep Aganyants,
  • Artur Hambardzumyan and ,
  • Tigran Soghomonyan,
  • Sona Avetisyan,
  • Sergey Kocharov,
  • Henry Panosyan,
  • Vehary Sakanyan and
  • Anichka Hovsepyan
  • + 5 authors+ 0 author

3 October 2024

The synthesis of enantiomeric forms of D-amino acids can be achieved by a two-step “hydantoinase process” based on the sequential catalysis of substrates by specific enzymes, D-carbamoylase and D-hydantoinase. Here, we describe the struct...

(This article belongs to the Topic Microbial Biotechnology Products and Biocatalysis Processes for a Sustainable Bioeconomy)
  • Article
  • Open Access
7 Citations
5,585 Views
19 Pages

Optimization of Sugar Extraction Process from Date Waste Using Full Factorial Design Toward Its Use for New Biotechnological Applications

  • Islam Sayah,
  • Mondher Njehi,
  • Nicola Cicero,
  • Vincenzo Nava and ,
  • Manel Ben M’hadheb,
  • Hatem Majdoub,
  • Sami Achour and
  • Teresa Gervasi
  • + 3 authors+ 0 author

3 October 2024

In Tunisia, the date industry generates a large quantity of waste, raising environmental concerns. However, dates are rich in sugars, which offer a renewable source of nutrients for various applications. In this study, sugar extraction from two low-g...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
  • Article
  • Open Access
10 Citations
4,088 Views
18 Pages

25 September 2024

Currently, starter cultures for fermenting plant-based beverages are not widely available commercially, but producers can use starter cultures for dairy products. Therefore, the aim of this study was to determine the physicochemical, rheological, ant...

(This article belongs to the Section Industry, Agriculture and Food Biotechnology)
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BioTech - ISSN 2673-6284