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BioTech, Volume 11, Issue 2

June 2022 - 13 articles

Cover Story: L-asparaginase (ASNase) is an aminohydrolase enzyme with significant applications in the pharmaceutical and food industries, notably in lymphoproliferative disorder treatment and in acrylamide reduction, and a potential carcinogenic compound found in heat-processed food products. However, due to the unstable nature of free enzymes and limitation to a single use, enzyme immobilization is a fascinating prospect to improve their properties. In this work, carbon xerogels with unique advantages, such as tunable size, high surface area, and strong loading capacity, were used for the first time as ASNase immobilization support. The immobilization conditions were optimized using a central composite design methodology, achieving exceptional immobilized ASNase recovered activities and immobilization yields, as well as improved operational and pH stabilities. View this paper
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Articles (13)

  • Article
  • Open Access
5 Citations
5,868 Views
15 Pages

This research aimed to investigate the effects of the intramuscular injection of vitamins AD3E and C in combination immediately before the estrus synchronization program (the Ovsynch program) on conception and pregnancy rates, blood parameters, serum...

  • Article
  • Open Access
4 Citations
6,040 Views
12 Pages

Post-translational modifications (“PTMs”) in monoclonal antibodies (mAbs) contribute to charge variant distribution, which will affect biological efficacy and safety. For the characterization of mAbs, charge variants are used as a critica...

  • Review
  • Open Access
13 Citations
10,320 Views
12 Pages

Burn injuries are among the most common peacetime injuries, with mortality ranging from 2.3% to 3.6%. At the same time, 85–90% of patients with burns are people of working age and children. Burn injury leads to metabolic disorders and systemic...

  • Article
  • Open Access
8 Citations
4,198 Views
11 Pages

Horizontal Transfer of Virulence Factors by Pathogenic Enterobacteria to Marine Saprotrophic Bacteria during Co-Cultivation in Biofilm

  • Alena I. Eskova,
  • Boris G. Andryukov,
  • Anatoli A. Yakovlev,
  • Alexandra V. Kim,
  • Anna L. Ponomareva and
  • Vera S. Obuhova

Environmental problems associated with marine pollution and climate warming create favorable conditions for the penetration and survival of pathogenic bacteria in marine ecosystems. These microorganisms have interspecific competitive interactions wit...

  • Article
  • Open Access
15 Citations
5,341 Views
9 Pages

Biochemical and Botanical Aspects of Allium sativum L. Sowing

  • Ali Ammarellou,
  • Ali Reza Yousefi,
  • Moslem Heydari,
  • Daniela Uberti and
  • Andrea Mastinu

The main aim of this study was to evaluate the yield and compliance of selected Iranian garlic (Allium sativum L.) cultivars, including Tuyserkan (TSN), Heydareh (HDH), Mouien (MUN), and Taroom (TRM), during two growing seasons. The TRM cultivar germ...

  • Article
  • Open Access
17 Citations
4,746 Views
12 Pages

Xylanase Production by Talaromyces amestolkiae Valuing Agroindustrial Byproducts

  • Giórgia S. Barbieri,
  • Heitor B. S. Bento,
  • Fernanda de Oliveira,
  • Flávio P. Picheli,
  • Lídia M. Dias,
  • Fernando Masarin and
  • Valéria C. Santos-Ebinuma

In general, agroindustrial byproducts can be easily assimilated by several microorganisms due to their composition, which is rich in carbohydrates. Therefore, they could be appropriate for use as raw materials in a sustainable refinery concept, inclu...

  • Article
  • Open Access
8 Citations
5,067 Views
16 Pages

A Freeze-Dried Cranberry Powder Consistently Enhances SCFA Production and Lowers Abundance of Opportunistic Pathogens In Vitro

  • Christina Khoo,
  • Cindy Duysburgh,
  • Massimo Marzorati,
  • Pieter Van den Abbeele and
  • Derek Zhang

The American cranberry, Vaccinium macrocarpon, contains fibers and (poly)phenols that could exert health-promoting effects through modulation of gut microbiota. This study aimed to investigate how a freeze-dried whole cranberry powder (FCP) modulated...

  • Article
  • Open Access
26 Citations
6,003 Views
15 Pages

Prebiotic Potential and Value-Added Products Derived from Spirulina laxissima SV001—A Step towards Healthy Living

  • Vidya Sankarapandian,
  • Kirubakaran Nitharsan,
  • Kavitha Parangusadoss,
  • Prakash Gangadaran,
  • Prasanna Ramani,
  • Balu Alagar Venmathi Maran and
  • Manasi P. Jogalekar

26 April 2022

Lately, microalgae-based value-added products have been gaining market value because they moderate the dependency on fossil fuel and high-value chemical products. To this end, the purpose of this study was to develop prebiotic products from the micro...

  • Feature Paper
  • Article
  • Open Access
28 Citations
6,030 Views
15 Pages

Characterisation of Omicron Variant during COVID-19 Pandemic and the Impact of Vaccination, Transmission Rate, Mortality, and Reinfection in South Africa, Germany, and Brazil

  • Carolina Ribeiro Xavier,
  • Rafael Sachetto Oliveira,
  • Vinícius da Fonseca Vieira,
  • Marcelo Lobosco and
  • Rodrigo Weber dos Santos

26 April 2022

Several variants of SARS-CoV-2 have been identified in different parts of the world, including Gamma, detected in Brazil, Delta, detected in India, and the recent Omicron variant, detected in South Africa. The emergence of a new variant is a cause of...

  • Article
  • Open Access
14 Citations
4,506 Views
9 Pages

Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula

  • Juan Manuel Vélez-Ixta,
  • Tizziani Benítez-Guerrero,
  • Arlene Aguilera-Hernández,
  • Helga Martínez-Corona,
  • Karina Corona-Cervantes,
  • Carmen Josefina Juárez-Castelán,
  • Martín Noé Rangel-Calvillo and
  • Jaime García-Mena

20 April 2022

Mammary gland secretory cells produce miRNA-rich milk. In humans, these miRNAs reach infant/neonate bloodstream, playing diverse roles, like neural system development, metabolism, and immune system maturation. Notwithstanding, still few works explore...

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BioTech - ISSN 2673-6284