Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula
Abstract
:1. Introduction
2. Materials and Methods
2.1. Type of Study
2.2. Sample
2.3. Oligonucleotide Selection and Design
2.4. RNA Extraction
2.5. cDNA Synthesis
2.6. miRNA Quantification by qPCR
2.7. Bioinformatics and Statistical Analyses
3. Results
3.1. miRNA Relative Expression Is Comparable among the Studied Human Milk Samples
3.2. Selected miRNAs Were Identified in Infant Milk Formulae
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Anthropometric Data | Mean | SD | |
Age (years) | 22.93 | ±4.95 | |
Height (m) α | 1.57 | ±0.07 | |
Weight (Kg) β | 56.48 | ±10.01 | |
BMI γ | 23.22 | ±3.74 | |
Type of delivery | n | % | |
Elective caesarean | 4 | 6.67 | |
Non elective caesarean | 13 | 21.67 | |
Vaginal | 43 | 71.66 | |
Parity | n | % | |
Primiparous δ | 16 | 27.12 | |
Multiparous δ | 43 | 72.88 | |
Milk extraction | n | % | |
Manual ε | 34 | 82.93 | |
Pump ε | 7 | 17.07 | |
Socioeconomic data | n | % | |
Residence | Mexico City (19°25′10″ N 99°08′44″ O) | 15 | 25.00 |
Estado de México (19°21′15″ N 99°37′51″ O) | 36 | 60.00 | |
Guerrero State (17°36′47″ N 99°57′00″ O) | 2 | 3.33 | |
Hidalgo State (20°28′42″ N 98°51′49″ O) | 1 | 1.67 | |
Oaxaca State (16°53′53″ N 96°24′51″ O) | 3 | 5.00 | |
Puebla State (19°00′13″ N 97°53′18″ O) | 2 | 3.33 | |
Veracruz State (19°26′05″ N 96°22′59″ O) | 1 | 1.67 | |
Activity | Housewife δ | 52 | 88.14 |
Student δ | 2 | 3.39 | |
General employee δ | 5 | 8.47 | |
Education | Primary school (6 years) | 20 | 33.33% |
Secondary school (3 years) | 36 | 60.00% | |
High school (3 years) | 1 | 1.67% | |
University school (4–5 years) | 3 | 5.00% |
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Vélez-Ixta, J.M.; Benítez-Guerrero, T.; Aguilera-Hernández, A.; Martínez-Corona, H.; Corona-Cervantes, K.; Juárez-Castelán, C.J.; Rangel-Calvillo, M.N.; García-Mena, J. Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula. BioTech 2022, 11, 11. https://doi.org/10.3390/biotech11020011
Vélez-Ixta JM, Benítez-Guerrero T, Aguilera-Hernández A, Martínez-Corona H, Corona-Cervantes K, Juárez-Castelán CJ, Rangel-Calvillo MN, García-Mena J. Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula. BioTech. 2022; 11(2):11. https://doi.org/10.3390/biotech11020011
Chicago/Turabian StyleVélez-Ixta, Juan Manuel, Tizziani Benítez-Guerrero, Arlene Aguilera-Hernández, Helga Martínez-Corona, Karina Corona-Cervantes, Carmen Josefina Juárez-Castelán, Martín Noé Rangel-Calvillo, and Jaime García-Mena. 2022. "Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula" BioTech 11, no. 2: 11. https://doi.org/10.3390/biotech11020011