Methods Protoc., Volume 3, Issue 1 (March 2020) – 24 articles
Cover Story (view full-size image): Mono- and diferuloyl glycerol are naturally occurring phenylpropenoids that provide ultraviolet and antioxidant protection to pollen, spores, and waxy leaves in plants. These glycerol derivatives have potential use as cosmeceutical and agricultural adjuvants. We have developed a process that uses a continuously fed, packed bed, enzymatic bioreactor employing a commercial lipase to convert ethyl ferulate and vegetable oils to feruloylated vegetable oils. Under optimal conditions, the minor products from this process, mono- and diferuloyl glycerol, can be selectively produced in higher yield. We describe the flash chromatography purification of these natural glycerol derivatives in >10 g yields, an improvement over the milligram to low gram quantities obtained by preparative HPLC and column chromatography methods reported to date.View this paper.
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