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Methods and Protocols, Volume 3, Issue 1

March 2020 - 24 articles

Cover Story: Mono- and diferuloyl glycerol are naturally occurring phenylpropenoids that provide ultraviolet and antioxidant protection to pollen, spores, and waxy leaves in plants. These glycerol derivatives have potential use as cosmeceutical and agricultural adjuvants. We have developed a process that uses a continuously fed, packed bed, enzymatic bioreactor employing a commercial lipase to convert ethyl ferulate and vegetable oils to feruloylated vegetable oils. Under optimal conditions, the minor products from this process, mono- and diferuloyl glycerol, can be selectively produced in higher yield. We describe the flash chromatography purification of these natural glycerol derivatives in >10 g yields, an improvement over the milligram to low gram quantities obtained by preparative HPLC and column chromatography methods reported to date.View this paper.
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Articles (24)

  • Protocol
  • Open Access
20 Citations
4,601 Views
13 Pages

In this paper, an analytical protocol was developed for the simultaneous determination of phthalates (di-methyl phthalate DMP, di-ethyl phthalate DEP, di-isobutyl phthalate DiBP, di-n-butyl phthalate DBP, bis-(2-ethylhexyl) phthalate DEHP, di-n-octyl...

  • Article
  • Open Access
5 Citations
3,318 Views
12 Pages

The advent of next-generation sequencing has allowed for higher-throughput determination of which species live within a specific location. Here we establish that three analysis methods for estimating diversity within samples—namely, Operational Taxon...

  • Article
  • Open Access
25 Citations
10,678 Views
11 Pages

The assessment of minimum inhibitory concentration (MIC) is a conventional technique used for the screening of microbial resistance against antibiotics, biocides, and contaminants such as heavy metals. However, as part of our ongoing work, we have ob...

  • Article
  • Open Access
13 Citations
10,418 Views
23 Pages

27 February 2020

The loess-paleosol archive from Mircea Vodă (Romania) represents one of the most studied sections in Europe. We are applying here the current state of the art luminescence dating protocols for revisiting the chronology of this section. Analysis were...

  • Article
  • Open Access
1 Citations
3,148 Views
19 Pages

Sedimentary Dosimetry for the Saradj-Chuko Grotto: A Cave in a Lava Tube in the North-Central Caucasus, Russia

  • Bonnie A. B. Blackwell,
  • Mehak F. Kazi,
  • Clara L. C. Huang,
  • Ekaterina V. Doronicheva,
  • Liubov V. Golovanova,
  • Vladimir B. Doronichev,
  • Impreet K. C. Singh and
  • Joel I. B. Blickstein

26 February 2020

Karst caves host most European Paleolithic sites. Near the Eurasian-Arabian Plate convergence in the Caucasus’ Lower Chegem Formation, Saradj-Chuko Grotto (SCG), a lava tube, contains 16 geoarchaeologically distinct horizons yielding modern to...

  • Protocol
  • Open Access
8 Citations
6,064 Views
8 Pages

Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management

  • Odile Gabay,
  • Jonathan Vicenty,
  • Dylan Smith,
  • Linda Tiffany,
  • Jill Ascher,
  • Tina Curry,
  • John Dennis and
  • Kathleen A. Clouse

22 February 2020

The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease o...

  • Protocol
  • Open Access
14 Citations
4,978 Views
20 Pages

20 February 2020

Dating fluvial terraces has long been a challenge for geologists and geomorphologists, because terrace straths and treads are not usually directly dated. In this study, the formation ages of the Yellow River terraces in the Baode area in China were d...

  • Protocol
  • Open Access
1 Citations
4,114 Views
7 Pages

Antimicrobial Lock Therapy in Clinical Practice: A Scoping Review Protocol

  • Aniello Alfieri,
  • Sveva Di Franco,
  • Maria Beatrice Passavanti,
  • Maria Caterina Pace,
  • Agata Stanga,
  • Vittorio Simeon,
  • Paolo Chiodini,
  • Sebastiano Leone,
  • Vettakkara Kandy Muhammed Niyas and
  • Marco Fiore

12 February 2020

Our objective is to review the scientific literature on the use of antimicrobial lock therapy (ALT). To achieve this result, our scoping review will address the following seven key questions: (1) Who are the patients who will benefit from this techni...

  • Protocol
  • Open Access
7 Citations
3,373 Views
8 Pages

A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS

  • Antonio Brun,
  • Melisa E. Magallanes,
  • Carlos Martínez del Rio,
  • Gregory A. Barrett-Wilt,
  • William H. Karasov and
  • Enrique Caviedes-Vidal

10 February 2020

A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions. The gel was then incubated in substrate solutions...

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Methods Protoc. - ISSN 2409-9279