Next Article in Journal
Sedimentary Dosimetry for the Saradj-Chuko Grotto: A Cave in a Lava Tube in the North-Central Caucasus, Russia
Previous Article in Journal
Determining the Age of Terrace Formation Using Luminescence Dating—A Case of the Yellow River Terraces in the Baode Area, China
Open AccessProtocol

Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management

1
U.S. Food and Drug Administration, Center for Drug Evaluation and Research, Office of Biotechnology Products, Division of Biotechnology Review and Research 1, Silver Spring, MD 20993, USA
2
U.S. Food and Drug Administration, Center for Biologics Evaluation and Research, Office of Management, Division of Veterinary Services, Silver Spring, MD 20993, USA
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(1), 18; https://doi.org/10.3390/mps3010018
Received: 22 January 2020 / Revised: 19 February 2020 / Accepted: 20 February 2020 / Published: 22 February 2020
The particularly unique composition of the gut microbiota has the potential to influence the health or disease status of animal and human hosts. Altering the homeostasis of the host-bacteria could lead to changes in gut flora that result in disease or activation of a specific immunological response, which could explain the variations observed in patient responses to current therapies. A standardized model is crucial for studying the influence of the gut microbiota on therapeutic modalities. A step by step mouse model and sterility management system that compares a control strain of C57BL/6 mice to the established C57BL/6 germ-free (GF) strain has been developed. The GF BL/6 mouse phenotype is well established, and the anatomical differences between the GF and control mice were evident in this model. This method could be applied to research studies investigating the microbiome impact, the response to various therapies, or disease transfer via fecal transplants. A standardized sterility maintenance method is crucial in this context. View Full-Text
Keywords: microbiome; germ free; sterility method; animal model microbiome; germ free; sterility method; animal model
Show Figures

Figure 1

MDPI and ACS Style

Gabay, O.; Vicenty, J.; Smith, D.; Tiffany, L.; Ascher, J.; Curry, T.; Dennis, J.; Clouse, K.A. Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management. Methods Protoc. 2020, 3, 18. https://doi.org/10.3390/mps3010018

AMA Style

Gabay O, Vicenty J, Smith D, Tiffany L, Ascher J, Curry T, Dennis J, Clouse KA. Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management. Methods and Protocols. 2020; 3(1):18. https://doi.org/10.3390/mps3010018

Chicago/Turabian Style

Gabay, Odile; Vicenty, Jonathan; Smith, Dylan; Tiffany, Linda; Ascher, Jill; Curry, Tina; Dennis, John; Clouse, Kathleen A. 2020. "Using a Model of Germ-Free Animals to Study the Impact of Gut Microbiome in Research: A Step by Step Sterility Setting and Management" Methods Protoc. 3, no. 1: 18. https://doi.org/10.3390/mps3010018

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop