Next Article in Journal
Optically Stimulated Luminescence Sensitivity of Quartz for Provenance Analysis
Previous Article in Journal
Prevalence, Prevention and Treatment of Saddle Sores among Female Competitive Cyclists: A Scoping Review Protocol
Open AccessProtocol

Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies

1
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
2
Yale-NUS College, 10 College Avenue West, Singapore 138609, Singapore
*
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(1), 5; https://doi.org/10.3390/mps3010005
Received: 9 December 2019 / Revised: 4 January 2020 / Accepted: 6 January 2020 / Published: 10 January 2020
The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species.
Video sections
View Full-Text
Keywords: wing dissection; Bicyclus anynana; butterflies wing dissection; Bicyclus anynana; butterflies
Show Figures

Figure 1

MDPI and ACS Style

Banerjee, T.D.; Monteiro, A. Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies. Methods Protoc. 2020, 3, 5.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop