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Open AccessProtocol

Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies

Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
Yale-NUS College, 10 College Avenue West, Singapore 138609, Singapore
Author to whom correspondence should be addressed.
Methods Protoc. 2020, 3(1), 5;
Received: 9 December 2019 / Revised: 4 January 2020 / Accepted: 6 January 2020 / Published: 10 January 2020
The colorful wings of butterflies are emerging as model systems for evolutionary and developmental studies. Some of these studies focus on localizing gene transcripts and proteins in wings at the larval and pupal stages using techniques such as immunostaining and in situ hybridization. Other studies quantify mRNA expression levels or identify regions of open chromatin that are bound by proteins at different stages of wing development. All these techniques require dissection of the wings from the animal but a detailed video protocol describing this procedure has not been available until now. Here, we present a written and accompanying video protocol where we describe the tools and the method we use to remove the larval and pupal wings of the African Squinting Bush Brown butterfly Bicyclus anynana. This protocol should be easy to adapt to other species.
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Keywords: wing dissection; Bicyclus anynana; butterflies wing dissection; Bicyclus anynana; butterflies
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Banerjee, T.D.; Monteiro, A. Dissection of Larval and Pupal Wings of Bicyclus anynana Butterflies. Methods Protoc. 2020, 3, 5.

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