Bioengineering, Volume 9, Issue 12
2022 December - 101 articles
Cover Story: Cell-based treatments to replace lost or damaged insulin-secreting β cells is a promising approach in the treatment of diabetes. A favored method is to protect the cells within an implant device, and therefore the design of an internal environment within the implant that supports cell function would be beneficial. To test the impact of different environments we reaggregated isolated native pancreatic β cells to form small spheroids which we then embedded in alginate. We show that spheroids embedded in RGD-conjugated alginate orientate with respect to the outer surface with the enrichment of activated focal adhesions. Our functional assays show that glucose-dependent insulin secretion is increased with RGD-conjugated peptide compared to control alginate, and secretion is further enhanced in softer alginate gels. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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