Special Issue "Cell–ECM Interactions for Tissue Engineering and Tissue Regeneration"

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biochemical Engineering".

Deadline for manuscript submissions: 15 December 2022 | Viewed by 1373

Special Issue Editors

Dr. Ngan F. Huang
E-Mail Website
Guest Editor
Department of Cardiothoracic Surgery and Stanford Cardiovascular Institute, Stanford University, Palo Alto, CA 94305, USA
Interests: cardiovascular tissue engineering; biomaterials; extracellular matrix interactions; stem cell therapy; peripheral arterial disease; muscle regeneration
Special Issues, Collections and Topics in MDPI journals
Dr. Brandon J. Tefft
E-Mail Website
Guest Editor
Medical College of Wisconsin & Marquette University, Department of Biomedical Engineering, Milwaukee, WI 53226, USA
Interests: vascular tissue engineering; heart valve tissue engineering; nanotechnology; cardiovascular regenerative engineering; biomaterials; coronary heart disease; valvular heart disease

Special Issue Information

Dear Colleagues,

It is increasingly appreciated that the extracellular matrix (ECM) provides not only structural support, but also dynamic signaling cues that influence cellular behavior. In the context of tissue engineering and tissue regeneration, the ECM plays a critical role in many aspects, including the regulation of cell survival, cellular reorganization, contractility, as well as in the regulation of engineered cardiovascular tissue formation and function. This issue explores cellular interactions with naturally derived ECMs, biomimetic materials, and synthetic hydrogels. For example, the ECMs can take the form of 3D porous scaffolds, bioinks for 3D bioprinting, or decellularized ECMs. We invite manuscripts that focus on how cell–ECM interactions lead to advances in tissue engineering or in vivo tissue regeneration, as well as in the improved understanding of the underlying molecular biology.

Dr. Ngan F. Huang
Dr. Brandon J. Tefft
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • extracellular matrix
  • tissue engineering
  • bioprinting
  • decellularized matrix
  • tissue regeneration

Published Papers (1 paper)

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Human-Origin iPSC-Based Recellularization of Decellularized Whole Rat Livers
Bioengineering 2022, 9(5), 219; https://doi.org/10.3390/bioengineering9050219 - 19 May 2022
Viewed by 836
End-stage liver diseases lead to mortality of millions of patients, as the only treatment available is liver transplantation and donor scarcity means that patients have to wait long periods before receiving a new liver. In order to minimize donor organ scarcity, a promising [...] Read more.
End-stage liver diseases lead to mortality of millions of patients, as the only treatment available is liver transplantation and donor scarcity means that patients have to wait long periods before receiving a new liver. In order to minimize donor organ scarcity, a promising bioengineering approach is to decellularize livers that do not qualify for transplantation. Through decellularization, these organs can be used as scaffolds for developing new functional organs. In this process, the original cells of the organ are removed and ideally should be replaced by patient-specific cells to eliminate the risk of immune rejection. Induced pluripotent stem cells (iPSCs) are ideal candidates for developing patient-specific organs, yet the maturity and functionality of iPSC-derived cells do not match those of primary cells. In this study, we introduced iPSCs into decellularized rat liver scaffolds prior to the start of differentiation into hepatic lineages to maximize the exposure of iPSCs to native liver matrices. Through exposure to the unique composition and native 3D organization of the liver microenvironment, as well as the more efficient perfusion culture throughout the differentiation process, iPSC differentiation into hepatocyte-like cells was enhanced. The resulting cells showed significantly higher expression of mature hepatocyte markers, including important CYP450 enzymes, along with lower expression of fetal markers, such as AFP. Importantly, the gene expression profile throughout the different stages of differentiation was more similar to native development. Our study shows that the native 3D liver microenvironment has a pivotal role to play in the development of human-origin hepatocyte-like cells with more mature characteristics. Full article
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