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Separations, Volume 8, Issue 6

2021 June - 18 articles

Cover Story: Based on chromatographic properties of native and W26-modified chicken alpha 1-acid glycoprotein (cAGP) columns and docking simulations of studied compounds into the generated model structure of cAGP, the chiral binding sites and mechanism were discussed. Benzoin and chlorpheniramine enantiomers interacted with cAGP at almost the same sites a little away from W26, while propranolol enantiomers docked, slightly shifting toward H25 and W26. Furthermore, in addition to hydrophobic interactions, ionic interactions between amino groups of chlorpheniramine enantiomers and a carboxyl group of D161 or E168 played an important role in chiral recognition, while hydrophobic and hydrogen bonding interactions worked for the chiral recognition of benzoin and propranolol enantiomers. View this paper
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Articles (18)

  • Feature Paper
  • Article
  • Open Access
12 Citations
3,327 Views
13 Pages

Triacylglycerols (TAGs), as the main components of edible oils and animal fats, are responsible for the nutritional value, organoleptic features and technological properties of foods; each lipid matrix shows a unique TAG profile which can serve as fi...

  • Feature Paper
  • Article
  • Open Access
12 Citations
3,709 Views
18 Pages

In the Arctic zone, where up to 1024 × 1013 kg of organic matter is stored in permafrost-affected soils, soil organic matter consists of about 50% humic substances. Based on the analysis of the molecular composition of humic acids, we assessed the pr...

  • Article
  • Open Access
7 Citations
5,281 Views
14 Pages

Identification of Abnormal Proteins in Plasma from Gout Patients by LC-MS/MS

  • Lijin Shen,
  • Hanyang Dong,
  • Zhenchang Guo,
  • Guijin Zhai and
  • Kai Zhang

A high level of uric acid may cause hyperuricemia, which further develops into gout, eventually leading to chronic kidney disease. However, the pathogenic mechanism remains largely unknown. To investigate the cause and block the transformation of hyp...

  • Article
  • Open Access
12 Citations
5,681 Views
16 Pages

A Rapid HPLC Method for the Concurrent Determination of Several Antihypertensive Drugs from Binary and Ternary Formulations

  • Mahesh Attimarad,
  • Katharigatta N. Venugopala,
  • Nagaraja Sreeharsha,
  • Muhammad S. Chohan,
  • Sheeba Shafi,
  • Anroop B. Nair and
  • Shinu Pottathil

A rapid, synchronized liquid chromatographic method was established for the estimation of hydrochlorothiazide (HCZ), amlodipine (AMD), olmesartan (OLM), telmisartan (TEL), and irbesartan (IRB) in binary and ternary coformulations using the same chrom...

  • Feature Paper
  • Article
  • Open Access
18 Citations
5,012 Views
19 Pages

Automated Screening and Filtering Scripts for GC×GC-TOFMS Metabolomics Data

  • Seo Lin Nam,
  • A. Paulina de la Mata and
  • James J. Harynuk

Comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS) is a powerful tool for the analysis of complex mixtures, and it is ideally suited to discovery studies where the entire sample is potentially of interest. Unfortunately, wh...

  • Communication
  • Open Access
10 Citations
4,941 Views
6 Pages

Fluorometric Optimized Determination of Total Glutathione in Erythrocytes

  • Antonio Francioso,
  • Sergio Fanelli,
  • Rosaria A. Cavallaro,
  • Mario Fontana,
  • Roberto Mattioli,
  • Maria D’Erme and
  • Luciana Mosca

Glutathione is a tripeptide natural product characterized by a non-canonical peptide bond with an amide moiety linking the nitrogen of cysteine to the γ-carboxyl of glutamate, and is found ubiquitously in nature, in animals, plants and microorganisms...

  • Article
  • Open Access
19 Citations
6,394 Views
21 Pages

Analysis of Unusual Sulfated Constituents and Anti-infective Properties of Two Indonesian Mangroves, Lumnitzera littorea and Lumnitzera racemosa (Combretaceae)

  • Jeprianto Manurung,
  • Jonas Kappen,
  • Jan Schnitzler,
  • Andrej Frolov,
  • Ludger A. Wessjohann,
  • Andria Agusta,
  • Alexandra N. Muellner-Riehl and
  • Katrin Franke

Lumnitzera littorea and Lumnitzera racemosa are mangrove species distributed widely along the Indonesian coasts. Besides their ecological importance, both are of interest owing to their wealth of natural products, some of which constitute potential s...

  • Feature Paper
  • Article
  • Open Access
17 Citations
3,451 Views
14 Pages

Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS

  • José Vera,
  • Virgínia Cruz Fernandes,
  • Luísa Correia-Sá,
  • Catarina Mansilha,
  • Cristina Delerue-Matos and
  • Valentina F. Domingues

A multiresidue analytical methodology based on a solid-phase microextraction (SPME), followed by gas chromatography-ion trap mass spectrometry (GC–IT/MS), has been developed for trace analysis of 20 known or suspected endocrine-disrupting pesticides....

  • Article
  • Open Access
20 Citations
6,236 Views
11 Pages

Development and Validation of an Analytical Method for Deacetylasperulosidic Acid, Asperulosidic Acid, Scopolin, Asperuloside and Scopoletin in Fermented Morinda citrifolia L. (Noni)

  • Sun-Il Choi,
  • Hee-Yeon Kwon,
  • Im-Joung La,
  • Yeon-Hui Jo,
  • Xionggao Han,
  • Xiao Men,
  • Se-Jeong Lee,
  • Yong-Deok Kim,
  • Geum-Su Seong and
  • Ok-Hwan Lee

Fermentation is a technology that enhances biologically active ingredients, improves the absorption rate and induces the generation of new functional ingredients by the catalytic action of enzyme systems possessed by microorganisms. In this study, ch...

  • Article
  • Open Access
6 Citations
4,309 Views
15 Pages

In view of the difficulty of traditional hydrocyclones to meet the requirements of fine classification, a double-overflow three-product (internal overflow, external overflow and underflow) hydrocyclone was designed in this study. Numerical simulation...

  • Article
  • Open Access
16 Citations
10,174 Views
10 Pages

Smokeless tobacco products and nicotine-containing tobacco-free oral pouches have increased in popularity in recent years. They are associated with far fewer health hazards compared to cigarettes. Nicotine pouches are filled with non-tobacco filler a...

  • Feature Paper
  • Article
  • Open Access
7 Citations
7,247 Views
20 Pages

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major cl...

  • Article
  • Open Access
2 Citations
4,020 Views
11 Pages

In this study, we conducted the characterization and purification of the thermostable mannitol dehydrogenase (MtDH) from Caldicellulosiruptor hydrothermalis 108. Furthermore, a coupling-enzyme system was designed using (MtDH) from Caldicellulosirupto...

  • Communication
  • Open Access
7 Citations
3,625 Views
7 Pages

Heterologous Biosynthesis of Artemisinin in Chrysanthemum morifolium Ramat

  • Aleksey Firsov,
  • Alexander Pushin,
  • Svetlana Motyleva,
  • Svetlana Pigoleva,
  • Lyubov Shaloiko,
  • Alexander Vainstein and
  • Sergey Dolgov

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plasmodium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amoun...

  • Article
  • Open Access
11 Citations
4,203 Views
17 Pages

The optimization of process parameters in the pharmaceutical industry is often carried out according to the Quality by Design (QbD) concept. QbD also emphasizes that continuous improvement should be performed in life cycle management. Process paramet...

  • Feature Paper
  • Article
  • Open Access
9 Citations
3,864 Views
10 Pages

Chiral stationary phases based on chicken alpha 1-acid glycoprotein (cAGP) have been used for enantioseparations of various compounds. However, the chiral binding sites and mechanism have not been clarified yet. Based on chromatographic properties of...

  • Article
  • Open Access
24 Citations
7,354 Views
26 Pages

The effect of mineral dust in the air sucked in by an engine on accelerated component wear and reduction in performance was presented. The necessity to use two-stage air filters (multicyclone-paper insert) for military vehicles was shown. The results...

  • Article
  • Open Access
1 Citations
2,672 Views
9 Pages

Apatite is an important carrier of rare earth elements (REEs) and phosphorite is a potential REEs resource. However, the influence of hydrothermal fluids on the migration and enrichment of REE in apatite remains controversial. The experimental study...

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Separations - ISSN 2297-8739