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Separations, Volume 8, Issue 5

2021 May - 16 articles

Cover Story: Mitotane (DDD) is prescribed in adrenocortical renal carcinoma. Its principal metabolite, dichlorodiphenylethene (DDE), can accumulate in fat tissues and, from a toxicological point of view, is probably more interesting than the other metabolite, dichlorodiphenylacetate (DDA). Therapeutic drug monitoring (TDM) of DDD plasma concentrations is required to combine therapeutic efficacy with acceptable toxicity. Therefore, we developed a simple and fast HPLC-UV method to monitor plasma concentrations after a liquid–liquid extraction of plasma samples, quality controls, and anonymous plasma samples with unknown DDD and DDE concentrations. The method was successfully validated according to the FDA guidelines and adopted for routine TDM. View this paper
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Articles (16)

  • Article
  • Open Access
5 Citations
4,829 Views
15 Pages

Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling

  • Stefano Dugheri,
  • Nicola Mucci,
  • Enrico Mini,
  • Donato Squillaci,
  • Giorgio Marrubini,
  • Gianluca Bartolucci,
  • Elisabetta Bucaletti,
  • Giovanni Cappelli,
  • Lucia Trevisani and
  • Giulio Arcangeli

Platinum-based antineoplastic drugs (PtADs) are among the most important and used families of chemotherapy drugs, which, even showing severe side effects and being hindered by drug resistance, are not likely to be replaced clinically any time soon. T...

  • Feature Paper
  • Article
  • Open Access
7 Citations
3,424 Views
13 Pages

New Concept for the Study of the Fluid Dynamics of Lithium Extraction Using Calix[4]arene Derivatives in T-Type Microreactor Systems

  • Yehezkiel Steven Kurniawan,
  • Ramachandra Rao Sathuluri,
  • Keisuke Ohto,
  • Wataru Iwasaki,
  • Hidetaka Kawakita,
  • Shintaro Morisada,
  • Masaya Miyazaki and
  • Jumina Jumina

Lithium extraction remains a challenge in the hydrometallurgy process due to its economic value and maldistribution sources. Employing calix[4]arene derivatives in solvent extraction techniques results in high selectivity and extraction capability, b...

  • Article
  • Open Access
14 Citations
4,062 Views
16 Pages

Eco-Friendly Reduced Graphene Oxide Nanofilter Preparation and Application for Iron Removal

  • Pankaj Kumar Jha,
  • Watsa Khongnakorn,
  • Chamorn Chawenjkigwanich,
  • Md Shahariar Chowdhury and
  • Kuaanan Techato

In this paper, the green synthesis of reduced graphene oxide (r-GO) nanomaterials using Callistemon viminalis leaf extract as a reducing and stabilizing agent is reported for the first time. The synthesized r-GO nanomaterials were characterized using...

  • Article
  • Open Access
1 Citations
4,065 Views
9 Pages

Rapid Production of PDMS Microdevices for Electrodriven Separations and Microfluidics by 3D-Printed Scaffold Removal

  • Alena Šustková,
  • Klára Konderlová,
  • Ester Drastíková,
  • Stefan Sützl,
  • Lenka Hárendarčíková and
  • Jan Petr

In our work, we produced PDMS-based microfluidic devices by mechanical removal of 3D-printed scaffolds inserted in PDMS. Two setups leading to the fabrication of monolithic PDMS-based microdevices and bonded (or stamped) PDMS-based microdevices were...

  • Article
  • Open Access
11 Citations
7,739 Views
18 Pages

Next-Generation Dried Blood Spot Samplers for Protein Analysis: Describing Trypsin-Modified Smart Sampling Paper

  • Eleonora Pizzi,
  • Trine Grønhaug Halvorsen,
  • Christian J. Koehler and
  • Léon Reubsaet

This paper describes smart sampling paper to be used for bottom-up protein analysis. Four different manners to immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized paper (with and without post-immobiliza...

  • Feature Paper
  • Article
  • Open Access
3 Citations
3,285 Views
10 Pages

When hydraulic pressure was added on the feed side of the membrane in the otherwise conventional pressure retarded osmosis (PRO) process, the production rate of the salinity gradient energy could be significantly increased by manipulating the hydraul...

  • Feature Paper
  • Review
  • Open Access
60 Citations
11,188 Views
25 Pages

Advanced Analytical Approaches for the Analysis of Polyphenols in Plants Matrices—A Review

  • Elena Roxana Chiriac,
  • Carmen Lidia Chiţescu,
  • Elisabeta-Irina Geană,
  • Cerasela Elena Gird,
  • Radu Petre Socoteanu and
  • Rica Boscencu

Phenolic compounds are plants’ bioactive metabolites that have been studied for their ability to confer extensive benefits to human health. As currently there is an increased interest in natural compounds identification and characterization, new anal...

  • Article
  • Open Access
33 Citations
8,320 Views
26 Pages

LC-ESI-QTOF-MS/MS Profiling and Antioxidant Activity of Phenolics from Custard Apple Fruit and By-Products

  • Junxi Du,
  • Biming Zhong,
  • Vigasini Subbiah,
  • Colin J. Barrow,
  • Frank R. Dunshea and
  • Hafiz A. R. Suleria

Custard apple is an edible fruit grown in tropical and subtropical regions. Due to its abundant nutrient content and perceived health benefits, it is a popular food for consumption and is utilized as a medicinal aid. Although some published research...

  • Feature Paper
  • Article
  • Open Access
3 Citations
4,351 Views
10 Pages

Determination of Mitotane (DDD) and Principal Metabolite by a Simple HPLC-UV Method and Its Validation in Human Plasma Samples

  • Giacomo Luci,
  • Federico Cucchiara,
  • Laura Ciofi,
  • Francesca Mattioli,
  • Marianna Lastella,
  • Romano Danesi and
  • Antonello Di Paolo

Mitotane (DDD) is prescribed in adrenocortical renal carcinoma. Its principal metabolite, dichlorodiphenylethene (DDE), can accumulate in fat tissues and from a toxicological point of view, is probably more interesting than the other metabolite dichl...

  • Article
  • Open Access
2 Citations
3,189 Views
14 Pages

Concentrated growth factors are extracted from platelet-rich plasma obtained from healthy adult veins by physical gradient centrifugation, and the activated platelets release various growth factors and cytokines, which can be further converted into c...

  • Feature Paper
  • Review
  • Open Access
24 Citations
5,728 Views
21 Pages

Metal-Organic Frameworks in Bioanalysis: Extraction of Small Organic Molecules

  • Natalia Manousi,
  • Orfeas-Evangelos Plastiras,
  • Natasa P. Kalogiouri,
  • Constantinos K. Zacharis and
  • George A. Zachariadis

The quantitative determination of xenobiotic compounds, as well as biotics in biological matrices, is generally described with the term bioanalysis. Due to the complexity of biofluids, in combination with the low concentration of the small molecules,...

  • Feature Paper
  • Article
  • Open Access
35 Citations
4,478 Views
19 Pages

Andean Blueberry of the Genus Disterigma: A High-Resolution Mass Spectrometric Approach for the Comprehensive Characterization of Phenolic Compounds

  • Sara Elsa Aita,
  • Anna Laura Capriotti,
  • Chiara Cavaliere,
  • Andrea Cerrato,
  • Benedetta Giannelli Moneta,
  • Carmela Maria Montone,
  • Susy Piovesana and
  • Aldo Laganà

Wild neotropical blueberries, endemic of Central and South American areas, are promising yet still undisclosed sources of bioactive compounds. Most research studies have addressed wild and cultivated blueberries from Europe and North America, despite...

  • Feature Paper
  • Review
  • Open Access
6 Citations
3,887 Views
12 Pages

Many efforts have been made to separate basic compounds, which are challenging to resolve in reversed phase liquid chromatography. In this process, they are strongly retained and the peak shape undergoes significant distortion. The principal origin o...

  • Article
  • Open Access
21 Citations
5,753 Views
9 Pages

In-Vitro Evaluation of the Antioxidant and Anti-Inflammatory Activity of Volatile Compounds and Minerals in Five Different Onion Varieties

  • Rokayya Sami,
  • Abeer Elhakem,
  • Mona Alharbi,
  • Manal Almatrafi,
  • Nada Benajiba,
  • Taha Ahmed Mohamed,
  • Mohammad Fikry and
  • Mahmoud Helal

Onions contain high antioxidants compounds that fight inflammation against many diseases. The purpose was to investigate some selected bioactive activities of onion varieties (Yellow, Red, Green, Leek, and Baby). Antioxidant assays and anti-inflammat...

  • Feature Paper
  • Article
  • Open Access
7 Citations
4,453 Views
16 Pages

Sintered Glass Monoliths as Supports for Affinity Columns

  • Marco Wilke,
  • Bettina Röder,
  • Martin Paul and
  • Michael G. Weller

A novel stationary phase for affinity separations is presented. This material is based on sintered borosilicate glass readily available as semi-finished filter plates with defined porosity and surface area. The material shows fast binding kinetics an...

  • Article
  • Open Access
8 Citations
4,549 Views
14 Pages

Fortified Cold-Pressed Oils: The Effect on Sensory Quality and Functional Properties

  • Alexandra Tauferova,
  • Dani Dordevic,
  • Simona Jancikova,
  • Bohuslava Tremlova and
  • Piotr Kulawik

The aim of this study was to monitor sensory quality, stability, selected nutritionally interesting properties and their changes in cold-pressed oil blends after fortification with chia and sesame seeds and seed oils during repeated thermal treatment...

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Separations - ISSN 2297-8739