Plants, Volume 12, Issue 24
2023 December-2 - 121 articles
Cover Story: The transport of metals such as iron (Fe), manganese (Mn), and cadmium (Cd) in rice is highly related. Although Fe and Mn are essential for plant growth, Cd is toxic for plants and humans. OsNRAMP5 is responsible for transporting Mn and Cd from the soil to rice. A knockout of OsNRAMP5 markedly reduces both Cd and Mn absorption, which is indispensable for developing low-Cd rice, but in low-Mn environments, such plants would exhibit Mn deficiency. We generated random mutations in OsNRAMP5 using error-prone PCR and used yeast to screen for the absorption of Mn and Cd. The results showed that alanine 512th is the most important amino acid residue for Cd absorption; its substitution resulted in the absorption of Mn but not Cd, and such a cytoplasmic mutation changed the protein structure. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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