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Biosensors, Volume 15, Issue 5

May 2025 - 69 articles

Cover Story: We present a graphene–quantum dot (QD) hybrid biosensor that achieves femtomolar sensitivity through a charge transfer-based quenching and recovery mechanism. Using single-layer graphene field-effect transistors (SLG-FETs) and time-resolved photoluminescence (TRPL), we demonstrate that photoluminescence quenching in QD–graphene hybrids results from static charge transfer rather than energy transfer. A simple analytical quantum mechanical model supports this interpretation. Electrical and optical signals show correlated responses to analyte concentration, enabling dual-mode detection. We validated the platform for biotin–streptavidin and IgG–anti-IgG interactions, achieving limits of detection down to 0.1 fM. These results establish a robust framework for next-generation, high-specificity biosensors. View this paper
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Articles (69)

  • Article
  • Open Access
1,044 Views
15 Pages

A Nanoparticle-Based Immunoassay on Facemasks for Evaluating Neutrophilic Airway Inflammation in COPD Patients

  • Bartomeu Mestre,
  • Nuria Toledo-Pons,
  • Andreu Vaquer,
  • Sofia Tejada,
  • Antonio Clemente,
  • Amanda Iglesias,
  • Meritxell López,
  • Ruth Engonga,
  • Sabina Perelló and
  • Borja G. Cosío
  • + 1 author

19 May 2025

Patients with chronic obstructive pulmonary disease (COPD) often experience acute exacerbations characterized by elevated neutrophilic inflammation in the lungs. Currently, this condition is diagnosed through visual inspection of sputum color and vol...

  • Article
  • Open Access
2 Citations
1,066 Views
12 Pages

18 May 2025

Kanamycin (KanR) is a widely used antibiotic in human and veterinary medicine, as well as in food production and livestock breeding. However, its environmental residue and bioaccumulation in the food chain pose a great threat to human health. A real-...

  • Article
  • Open Access
2 Citations
3,208 Views
15 Pages

A Highly Sensitive Electrochemical Immunosensor for Cortisol Detection

  • Pritu Parna Sarkar,
  • Ali Ashraf,
  • Ahmed Hasnain Jalal,
  • Fahmida Alam and
  • Nazmul Islam

17 May 2025

In this research, an interdigitated gear-shaped working electrode is presented for cortisol sensing. Overall, the sensor was designed in a three-electrode system and was fabricated using direct laser scribing. A synthesized conductive ink based on gr...

  • Article
  • Open Access
1,395 Views
16 Pages

MIP-Modified Porous Silicon Optical Sensor for Interleukin-6 Label-Free Quantification

  • Valeria Nocerino,
  • Giulia Siciliano,
  • Monica Bianco,
  • Ilaria Rea,
  • Principia Dardano,
  • Maria Serena Chiriacò,
  • Francesco Ferrara,
  • Giuseppe Gigli,
  • Elisabetta Primiceri and
  • Luca De Stefano

17 May 2025

In this study, we present an innovative optical biosensor designed to detect Interleukin-6 (IL-6), a pivotal cytokine implicated in many pathological conditions. Our sensing platform is made of a porous silicon (PSi) nanostructured substrate modified...

  • Article
  • Open Access
1 Citations
1,138 Views
11 Pages

Colorimetric Detection of microRNA-378 Based on Y-Shaped Structure Formed by Gold Nanoparticles and Catalytic Hairpin Self-Assembly

  • Yahui Gao,
  • Jinru Pan,
  • Bingyuan Fan,
  • Shan Wang,
  • Qian Wang,
  • Wanru Liu,
  • Fang Hu and
  • Wei Meng

15 May 2025

The timely and accurate detection of cancer is crucial for preventing disease progression and for the early treatment of confirmed cases. MiRNAs are cancer markers. In this study, a simple miRNA detection method is proposed. Three hairpins were desig...

  • Article
  • Open Access
1,159 Views
11 Pages

15 May 2025

Rolling circle amplification (RCA) at ambient temperature is prone to false positive signals during nucleic acid detection, which makes it challenging to establish an efficient RCA detection method. The false positive signals are primarily caused by...

  • Article
  • Open Access
1,291 Views
12 Pages

Development of Immunoassays for Foodborne Pathogenic Bacteria Detection Using PolyHRP for Signal Enhancement

  • Yijia Zhang,
  • Junkang Pan,
  • Qiyi He,
  • Zhihao Xu,
  • Bruce D. Hammock and
  • Dongyang Li

15 May 2025

The rapid and accurate detection of foodborne pathogens is essential for ensuring food safety. Escherichia coli O157:H7 (E. coli O157:H7) and Salmonella Typhimurium (S. Typhimurium) are major foodborne pathogenic bacteria that pose significant public...

  • Article
  • Open Access
1 Citations
1,916 Views
13 Pages

15 May 2025

The performance of heterophase immunoassays is often limited by the kinetics of analyte binding. This problem is partially solved by bead-based assays, which are characterized by rapid diffusion in the particle suspension. However, at low analyte con...

  • Article
  • Open Access
2 Citations
950 Views
15 Pages

14 May 2025

Copper-thiolate nanostructures, formed through the self-assembly of cysteine (Cys) and glutathione (GSH) with copper ions, offer a versatile platform for redox-active applications due to their structural stability and chemical functionality. In this...

  • Article
  • Open Access
3 Citations
1,443 Views
19 Pages

14 May 2025

Precise analysis of electroencephalogram (EEG) signals is critical for advancing the understanding of neurological conditions and mapping brain activity. However, accurately visualizing brain regions and behavioral patterns from neural signals remain...

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Biosensors - ISSN 2079-6374