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Open AccessCommunication

A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

1
Healthcare Technology Innovation Centre, Indian Institute of Technology, Madras, Chennai, Tamil Nadu 600113, India
2
Department of Electrical Engineering, Indian Institute of Technology, Chennai, Tamil Nadu 600113, India
3
IBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Biosensors 2021, 11(2), 49; https://doi.org/10.3390/bios11020049
Received: 5 January 2021 / Revised: 10 February 2021 / Accepted: 11 February 2021 / Published: 14 February 2021
(This article belongs to the Special Issue Cellulose-Based Biosensing Platforms)
This paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper. The results show that the wicking and test time are comparable to those obtained with conventional nitrocellulose (NC)-based LFAs. Further, the cellulose paper provides an excellent background with no auto-fluorescence that is very adequate in detecting fluorescent lines. While fluorescence that was generated with cellulose strips was lower when compared to that generated in NC strips, signals could be improved by layering carbon nanofibers (CNF) on the cellulose. A nonlinear behavior of the concentration–response relationship was observed for the LFA architectures with NC, cellulose, and cellulose-CNF in the 0 to 200 ng/mL cTnI concentration range. The measurements were consistent and characterized by coefficients of variation lower than 2.5%. Detection and quantitation limits that were in the range 1.28–1.40 ng/mL and 2.10–2.75 ng/mL were obtained for LFA with cellulose and cellulose CNF strips that are equivalent to the limits obtained with the standard NC LFA. Overall, we showed that commercially available filter paper can be used in the analytical strip of LFA. View Full-Text
Keywords: biomarker; carbon nanofiber; cellulose; diagnostics; immunoassay; lateral flow assays; paper; point-of-care testing; troponin I biomarker; carbon nanofiber; cellulose; diagnostics; immunoassay; lateral flow assays; paper; point-of-care testing; troponin I
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MDPI and ACS Style

Natarajan, S.; Jayaraj, J.; Prazeres, D.M.F. A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I. Biosensors 2021, 11, 49. https://doi.org/10.3390/bios11020049

AMA Style

Natarajan S, Jayaraj J, Prazeres DMF. A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I. Biosensors. 2021; 11(2):49. https://doi.org/10.3390/bios11020049

Chicago/Turabian Style

Natarajan, Satheesh; Jayaraj, Joseph; Prazeres, Duarte M.F. 2021. "A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I" Biosensors 11, no. 2: 49. https://doi.org/10.3390/bios11020049

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