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Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

1
Department of Chemical Engineering, The University of Toledo, Toledo, OH 43606, USA
2
Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA
*
Author to whom correspondence should be addressed.
Biosensors 2021, 11(2), 30; https://doi.org/10.3390/bios11020030
Received: 28 December 2020 / Revised: 12 January 2021 / Accepted: 19 January 2021 / Published: 24 January 2021
(This article belongs to the Special Issue Fluorescence Biosensors 2020)
Reactive oxygen species (ROS) have been found in plants, mammals, and natural environmental processes. The presence of ROS in mammals has been linked to the development of severe diseases, such as diabetes, cancer, tumors, and several neurodegenerative conditions. The most common ROS involved in human health are superoxide (O2), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Organic and inorganic molecules have been integrated with various methods to detect and monitor ROS for understanding the effect of their presence and concentration on diseases caused by oxidative stress. Among several techniques, fluorescence and electrochemical methods have been recently developed and employed for the detection of ROS. This literature review intends to critically discuss the development of these techniques to date, as well as their application for in vitro and in vivo ROS detection regarding free-radical-related diseases. Moreover, important insights into and further steps for using fluorescence and electrochemical methods in the detection of ROS are presented. View Full-Text
Keywords: reactive oxygen species; fluorescence sensor; electrochemical sensor; in vivo detection; oxidative-stress-related disease reactive oxygen species; fluorescence sensor; electrochemical sensor; in vivo detection; oxidative-stress-related disease
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MDPI and ACS Style

Duanghathaipornsuk, S.; Farrell, E.J.; Alba-Rubio, A.C.; Zelenay, P.; Kim, D.-S. Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications. Biosensors 2021, 11, 30. https://doi.org/10.3390/bios11020030

AMA Style

Duanghathaipornsuk S, Farrell EJ, Alba-Rubio AC, Zelenay P, Kim D-S. Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications. Biosensors. 2021; 11(2):30. https://doi.org/10.3390/bios11020030

Chicago/Turabian Style

Duanghathaipornsuk, Surachet; Farrell, Eveline J.; Alba-Rubio, Ana C.; Zelenay, Piotr; Kim, Dong-Shik. 2021. "Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications" Biosensors 11, no. 2: 30. https://doi.org/10.3390/bios11020030

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