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Biosensors, Volume 10, Issue 5

May 2020 - 11 articles

Cover Story: The sensitivity of aptamer-based assays is ultimately limited by the functional affinity of the synthetic receptors. In this work, we show how the use of isothermal nucleic acid amplification increases the sensitivity and the apparent affinity of aptamers, even when weak aptamers are employed. Specifically, we compare terminal deoxynucleotidyl transferase (TdT) and rolling circle amplification (RCA) in combination with two different aptamers against alpha-fetoprotein. Both DNA amplifications are useful although the improvement depends on the true affinity of the binding pair, which ultimately limits the analytical usefulness.View this paper.
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Articles (11)

  • Review
  • Open Access
54 Citations
10,565 Views
18 Pages

Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in env...

  • Article
  • Open Access
33 Citations
5,845 Views
13 Pages

The detection of adenine molecules at very low concentrations is important for biological and medical research and applications. This paper reports a silver-based surface-enhanced Raman scattering (SERS) sensor with a very low detection limit for ade...

  • Review
  • Open Access
43 Citations
10,182 Views
19 Pages

Biosensors are regarded as a powerful tool to detect and monitor environmental contaminants, toxins, and, more generally, organic or chemical markers of potential threats to human health. They are basically composed of a sensor part made up of either...

  • Article
  • Open Access
34 Citations
6,413 Views
14 Pages

Paper-Based Working Electrodes Coated with Mercury or Bismuth Films for Heavy Metals Determination

  • Alberto Sánchez-Calvo,
  • Maria Carmen Blanco-López and
  • Agustín Costa-García

Paper-based carbon working electrodes were modified with mercury or bismuth films for the determination of trace metals in aqueous solutions. Both modification procedures were optimized in terms of selectivity and sensitivity for the determination of...

  • Article
  • Open Access
11 Citations
5,422 Views
14 Pages

Fecal Volatile Organic Compound Profiles are Not Influenced by Gestational Age and Mode of Delivery: A Longitudinal Multicenter Cohort Study

  • Nancy Deianova,
  • Sofia el Manouni el Hassani,
  • Hendrik J. Niemarkt,
  • Veerle Cossey,
  • Anton H. van Kaam,
  • Floor Jenken,
  • Mirjam M. van Weissenbruch,
  • Esmee M. Doedes,
  • Kyra Baelde and
  • Renee Menezes
  • + 4 authors

Fecal volatile organic compounds (VOC) reflect human and gut microbiota metabolic pathways and their interaction. VOC behold potential as non-invasive preclinical diagnostic biomarkers in various diseases, e.g., necrotizing enterocolitis and late ons...

  • Article
  • Open Access
18 Citations
6,156 Views
11 Pages

The traditional qPCR instrument is bulky, expensive, and inconvenient to carry, so we report a portable rotary real-time fluorescent PCR (polymerase chain reaction) that completes the PCR amplification of DNA in the field, and the reaction can be obs...

  • Article
  • Open Access
8 Citations
5,433 Views
9 Pages

Smell—Adding a New Dimension to Urinalysis

  • Eva H. Visser,
  • Daan J. C. Berkhout,
  • Jiwanjot Singh,
  • Annemieke Vermeulen,
  • Niloufar Ashtiani,
  • Nanne K. de Boer,
  • Joanna A. E. van Wijk,
  • Tim G. de Meij and
  • Arend Bökenkamp

Background: Urinary tract infections (UTI) are among the most common infections in children. The primary tool to detect UTI is dipstick urinalysis; however, this has limited sensitivity and specificity. Therefore, urine culture has to be performed to...

  • Article
  • Open Access
11 Citations
4,682 Views
10 Pages

An Array of MOX Sensors and ANNs to Assess Grated Parmigiano Reggiano Cheese Packs’ Compliance with CFPR Guidelines

  • Marco Abbatangelo,
  • Estefanía Núñez-Carmona,
  • Veronica Sberveglieri,
  • Dario Zappa,
  • Elisabetta Comini and
  • Giorgio Sberveglieri

Parmigiano Reggiano cheese is one of the most appreciated Italian foods on account of its high nutrient content and taste. Due to its high cost, these characteristics make this product subject to counterfeiting in different forms. In this study, an a...

  • Article
  • Open Access
7 Citations
6,244 Views
11 Pages

On the Electrochemical Detection of Alpha-Fetoprotein Using Aptamers: DNA Isothermal Amplification Strategies to Improve the Performance of Weak Aptamers

  • Ramón Lorenzo-Gómez,
  • Daniel González-Robles,
  • Rebeca Miranda-Castro,
  • Noemí de-los-Santos-Álvarez and
  • María Jesús Lobo-Castañón

30 April 2020

Affinity characterization is essential to develop reliable aptamers for tumor biomarker detection. For alpha-fetoprotein (AFP), a biomarker of hepatocellular carcinoma (HCC), two DNA aptamers were described with very different affinity. In this work,...

  • Review
  • Open Access
147 Citations
21,006 Views
50 Pages

28 April 2020

The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the...

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Biosensors - ISSN 2079-6374