Table of Contents
Biosensors, Volume 10, Issue 5 (May 2020) – 11 articles
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Cover Story (view full-size image) The sensitivity of aptamer-based assays is ultimately limited by the functional affinity of the [...] Read more. The sensitivity of aptamer-based assays is ultimately limited by the functional affinity of the synthetic receptors. In this work, we show how the use of isothermal nucleic acid amplification increases the sensitivity and the apparent affinity of aptamers, even when weak aptamers are employed. Specifically, we compare terminal deoxynucleotidyl transferase (TdT) and rolling circle amplification (RCA) in combination with two different aptamers against alpha-fetoprotein. Both DNA amplifications are useful although the improvement depends on the true affinity of the binding pair, which ultimately limits the analytical usefulness.View this paper.