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Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview

1
Physics and Electronics, Department of Sciences and Technology, Linköping University, SE-601 74 Norrköping, Sweden
2
Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(5), 54; https://doi.org/10.3390/bios10050054
Received: 3 April 2020 / Revised: 11 May 2020 / Accepted: 13 May 2020 / Published: 18 May 2020
(This article belongs to the Special Issue Electrochemical Biosensors)
Escherichia coli O157:H7 (E. coli O157:H7) is a pathogenic strain of Escherichia coli which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic E. coli is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of E. coli O157:H7. This review covers the importance of E. coli O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for E. coli O157:H7 using different nanomaterials, labels, and electrochemical transducers. View Full-Text
Keywords: E. coli O157:H7; electrochemical biosensors; biomedical analysis; environmental monitoring; portable biodevice; biotechnology E. coli O157:H7; electrochemical biosensors; biomedical analysis; environmental monitoring; portable biodevice; biotechnology
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Razmi, N.; Hasanzadeh, M.; Willander, M.; Nur, O. Recent Progress on the Electrochemical Biosensing of Escherichia coli O157:H7: Material and Methods Overview. Biosensors 2020, 10, 54.

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