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Open AccessArticle

Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput

by Limin He 1,2, Benliang Sang 1,2 and Wenming Wu 1,2,*
1
State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun 130033, China
2
University of Chinese Academy of Sciences (UCAS), Beijing 100049, China
*
Author to whom correspondence should be addressed.
Biosensors 2020, 10(5), 49; https://doi.org/10.3390/bios10050049
Received: 15 April 2020 / Revised: 28 April 2020 / Accepted: 3 May 2020 / Published: 8 May 2020
(This article belongs to the Special Issue Fluorescence Biosensors 2020)
The traditional qPCR instrument is bulky, expensive, and inconvenient to carry, so we report a portable rotary real-time fluorescent PCR (polymerase chain reaction) that completes the PCR amplification of DNA in the field, and the reaction can be observed in real-time. Through the analysis of a target gene, namely pGEM-3Zf (+), the gradient amplification and melting curves are compared to commercial devices. The results confirm the stability of our device. This is the first use of a mechanical rotary structure to achieve gradient amplification curves and melting curves comparable to commercial instruments. The average power consumption of our system is about 7.6 W, which is the lowest energy consumption for real-time fluorescence quantification in shunting PCR and enables the use of our device in the field thanks to its self-contained power supply based on a lithium battery. In addition, all of the equipment costs only about 710 dollars, which is far lower than the cost of a commercial PCR instrument because the control system through mechanical displacement replaces the traditional TEC (thermoelectric cooler) temperature control. Moreover, the equipment has a low technical barrier, which can suit the needs of non-professional settings, with strong repeatability. View Full-Text
Keywords: real-time PCR; portable; rotary; low cost; low energy consumption; high throughput real-time PCR; portable; rotary; low cost; low energy consumption; high throughput
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He, L.; Sang, B.; Wu, W. Battery-Powered Portable Rotary Real-Time Fluorescent qPCR with Low Energy Consumption, Low Cost, and High Throughput. Biosensors 2020, 10, 49.

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