Animals

22 pages, 1673 KiB

Open AccessReview

Dairy Goat Production Systems: A Comprehensive Analysis to Reframe Their Global Diversity

by Cesar A. Meza-Herrera, Cayetano Navarrete-Molina, Ulises Macias-Cruz, Gerardo Arellano-Rodriguez, Angeles De Santiago-Miramontes, Maria A. Sariñana-Navarrete, Ruben I. Marin-Tinoco and Carlos C. Perez-Marin

Animals 2024, 14(24), 3717; https://doi.org/10.3390/ani14243717 - 23 Dec 2024

Viewed by 474

Abstract

This review aims to emphasize the important role that goats and dairy goats play for many small-scale rural families worldwide, as well as to introduce a proposal for categorizing the main dairy goat production systems (DGPSs), using a multifactorial approach but emphasizing rainfall [...] Read more.

This review aims to emphasize the important role that goats and dairy goats play for many small-scale rural families worldwide, as well as to introduce a proposal for categorizing the main dairy goat production systems (DGPSs), using a multifactorial approach but emphasizing rainfall and nutritional supplementation level, as the focal categorization factors. The main DGPSs were divided into two metasystems based on available resources, each consisting of three production subsystems. In the first metasystem, the three subsystems have limited water, biotic, and economic resources, whose main economic rationality is based on reducing risk rather than maximizing outputs. In contrast, the three subsystems of the second metasystem usually have increased biotic, economic, and water resources, whose main emphasis involves maximizing product yield rather than reducing risk. The first metasystem involves DGPSs with a very limited or null nutritional supplementation: (a) subsistence, (b) extensive, and (c) agro-silvopastoral. The second metasystem includes those DGPSs with different levels of nutritional supplementation: (d) semi-extensive, (e) semi-intensive, and (f) intensive. There are numerous significant global initiatives focused on scientific collaboration and sharing information regarding nutrition, reproductive, and genetic technologies related to the safety and nutraceutical quality of goat milk and products while contextualized in different DGPSs. Hence, such scenarios should create additional opportunities for researchers, producers, policymakers, and development workers to come together and align interests and needs and exchange knowledge on effective goat farmer support strategies, environmental management, and consumer education. Undoubtedly, it is essential to reevaluate the DGPSs in the world since millions of producers and their families—most of them poor and marginalized—need this species, society needs their products, most of the worldwide arid and semi-arid lands need their recovery, and all of us should encourage the fulfillment of the sustainable development goals. Full article

(This article belongs to the Section Small Ruminants)

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11 pages, 1418 KiB

Open AccessArticle

Novel Insertion/Deletion Polymorphisms and Genetic Studies of the Shadow of Prion Protein (SPRN) in Raccoon Dogs

by Da-In Choi, Mohammed Zayed, Eun-Jee Na, Jae-Ku Oem and Byung-Hoon Jeong

Animals 2024, 14(24), 3716; https://doi.org/10.3390/ani14243716 - 23 Dec 2024

Viewed by 540

Abstract

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. One of the candidate genes involved in prion diseases is the shadow of the prion protein (SPRN) gene. Raccoon dogs, a canid, are considered to be [...] Read more.

Prion diseases, or transmissible spongiform encephalopathies (TSEs), are a group of invariably fatal neurodegenerative disorders. One of the candidate genes involved in prion diseases is the shadow of the prion protein (SPRN) gene. Raccoon dogs, a canid, are considered to be a prion disease-resistant species. To date, the genetic polymorphisms of the SPRN gene and the predicted protein structure of the shadow of prion protein (Sho) have not been explored in raccoon dogs. SPRN was amplified using polymerase chain reaction (PCR). We also investigated the genetic polymorphisms of SPRN by analyzing the frequencies of genotypes, alleles, and haplotypes, as well as the linkage disequilibrium among the identified genetic variations. In addition, in silico analysis with MutPred-Indel was performed to predict the pathogenicity of insertion/deletion polymorphisms. Predicted 3D structures were analyzed by the Alphafold2. We found a total of two novel synonymous single nucleotide polymorphisms and three insertion/deletion polymorphisms. In addition, the 3D structure of the Sho protein in raccoon dogs was predicted to resemble that of the Sho protein in dogs. This is the first study regarding the genetic and structural characteristics of the raccoon dog SPRN gene. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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13 pages, 466 KiB

Open AccessArticle

Heritability Estimates of Age at First Calving and Correlation Analysis in Angus Cows Bred in Hungary

by Judit Márton, Szabolcs Albin Bene and Ferenc Szabó

Animals 2024, 14(24), 3715; https://doi.org/10.3390/ani14243715 - 23 Dec 2024

Viewed by 514

Abstract

This study aimed to examine the age at first calving (AFC) in Hungarian Angus herds. This study was conducted on the basis of data from 2955 registered cows, classified into five groups (based on different Angus types), and 200 breeding bulls, which were [...] Read more.

This study aimed to examine the age at first calving (AFC) in Hungarian Angus herds. This study was conducted on the basis of data from 2955 registered cows, classified into five groups (based on different Angus types), and 200 breeding bulls, which were the sires of the cows. The data were made available by the Hungarian Hereford, Angus, and Galloway Breeders’ Association. The variance and covariance components, heritability, breeding value (BV), and genetic trends of AFC between 1998 and 2021 were evaluated. A general linear model (univariate analysis of variance) was used to examine the various effects, while best linear unbiased prediction was used to estimate the population genetic parameters and BV, and linear regression analysis was used for the trend analysis. The average AFC obtained was 28.1 ± 0.1 months (SD = 5.3 months), showing a relatively large variance (CV = 18.9%). The environmental factors that influenced the development of the phenotype were the cow’s birth season (28.99%, p < 0.01), cow’s birth year (28.7%, p < 0.01), the cow’s sire (18.32%, p < 0.01), and the herd (11.77, p < 0.05). The cow’s color variant (8.10%, p > 0.05) was not significant and did not influence the AFC in this study. The direct heritability of AFC (h² = 0.51 ± 0.06) was higher than data in the literature (0.38 ± 0.05); however, the maternal heritability was low (h²_m = 0.00 ± 0.03). The correlation between direct and maternal genetic effects was zero (r_dm = −0.97 ± 1.00). The phenotypic trend of AFC increased by +0.03 months per year, which was not statistically significant. The genetic trend calculation showed no significant changes. Based on the h² and BV results, it seems that selecting a suitable sire can effectively reduce the AFC of daughters. Since AFC is also an important trait in economic terms, it would be appropriate to include AFC BV in the bull catalog. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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15 pages, 1208 KiB

Open AccessArticle

Improvements in Blood Profiles of Canines Naturally Infected with Triple Blood Pathogens (Babesia vogeli, Ehrlichia canis, and Anaplasma platys) Subsequent to Doxycycline Monotherapy

by Tuempong Wongtawan, Narin Sontigun, Kanpapat Boonchuay, Phatcharaporn Chiawwit, Oraphan Wongtawan, Orachun Hayakijkosol and Worakan Boonhoh

Animals 2024, 14(24), 3714; https://doi.org/10.3390/ani14243714 - 23 Dec 2024

Viewed by 759

Abstract

Multiple blood pathogen infections are increasingly found in many areas, particularly in tropical regions. This study aimed to investigate the effectiveness and safety of using doxycycline monotherapy to treat triple blood pathogen infection in sheltered dogs. A total of 375 sheltered dogs were [...] Read more.

Multiple blood pathogen infections are increasingly found in many areas, particularly in tropical regions. This study aimed to investigate the effectiveness and safety of using doxycycline monotherapy to treat triple blood pathogen infection in sheltered dogs. A total of 375 sheltered dogs were screened for blood pathogen using polymerase chain reaction technique (PCR). There were 34 dogs with triple infection (Babesia vogeli, Ehrlichia canis, and Anaplasma platys), and most dogs displayed anemia and thrombocytopenia. These dogs were treated with doxycycline (10 mg/kg/day) orally for four weeks, and their blood profiles were monitored. Almost all pathogens were undetectable by PCR by day 14 and pathogens were all cleared by day 28. Most blood profiles significantly improved after 14 days. The improvement continued after 28 days. The red blood cell and platelet count (PLT) were increased in similar trends between mild-to-moderate and severe thrombocytopenia groups. The average PLT in the mild-to-moderate thrombocytopenia group raised to normal level by day 14, whereas in the severe group, it was gradually increased to normal level by day 70. We propose using doxycycline monotherapy for 28 days to eliminate all pathogens, and facilitate recovery and welfare in dogs suffering with triple blood pathogen infections. Full article

(This article belongs to the Section Companion Animals)

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15 pages, 786 KiB

Open AccessArticle

Preserving Mediterranean Donkeys: A Study on Milk Production and Nutritional Benefits

by Mohamed Aroua, Antonella Fatica, Samia Ben Said, Mokhtar Mahouachi and Elisabetta Salimei

Animals 2024, 14(24), 3713; https://doi.org/10.3390/ani14243713 - 23 Dec 2024

Viewed by 393

Abstract

The aim of the study was to model lactation curves and assess the physicochemical properties, amino acid, and fatty acid profiles of milk from two Mediterranean donkey populations, Masri (n = 14) and North African (n = 14), using the Wood model. Over [...] Read more.

The aim of the study was to model lactation curves and assess the physicochemical properties, amino acid, and fatty acid profiles of milk from two Mediterranean donkey populations, Masri (n = 14) and North African (n = 14), using the Wood model. Over a lactation period of 205 ± 12.5 days, North African donkeys produced more milk (188.66 ± 7.19 kg) than Masri donkeys (163.42 ± 7.21 kg, p < 0.05). Peak milk yields occurred on day 57 for North African donkeys (1.212 kg/day) and day 59 for Masri donkeys (0.991 kg/day), with similar persistency indices of 7.19 and 7.21, respectively. North African donkey milk had significantly higher protein (1.45 ± 0.03 g/100 g) and β-lactoglobulin (4.75 ± 0.06 mg/mL) contents, while Masri donkey milk contained more fat (1.16 ± 0.05 g/100 g). Amino acid analysis revealed higher glutamate (0.27 ± 0.12 g/100 g) in North African donkey milk, while Masri donkey milk had more aspartate (0.16 ± 0.04 g/100 g). North African donkey milk had higher palmitic acid (20.1 ± 0.07 g/100 g), while Masri donkey milk had more oleic acid (21.4 ± 0.42 g/100 g). Lactation curve fitting yielded R² values of 93.8% for Masri donkeys and 95.7% for North African donkeys. These findings suggest that both populations are well-suited for milk production, particularly for human consumption or food applications. North African donkeys exhibited superior yields and nutrient profiles suitable for functional food applications. Full article

(This article belongs to the Special Issue Current Research on Donkeys and Mules)

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17 pages, 3699 KiB

Open AccessArticle

Efficient Convolutional Neural Network Model for the Taxonomy and Sex Identification of Three Phlebotomine Sandfly Species (Diptera, Psychodidae, and Phlebotominae)

by Mohammad Fraiwan

Animals 2024, 14(24), 3712; https://doi.org/10.3390/ani14243712 - 23 Dec 2024

Viewed by 594

Abstract

Sandflies, small insects primarily from the Psychodidae family, are commonly found in sandy, tropical, and subtropical regions. Most active during dawn and dusk, female sandflies feed on blood to facilitate egg production. In doing so, they can transmit infectious diseases that may cause [...] Read more.

Sandflies, small insects primarily from the Psychodidae family, are commonly found in sandy, tropical, and subtropical regions. Most active during dawn and dusk, female sandflies feed on blood to facilitate egg production. In doing so, they can transmit infectious diseases that may cause symptoms such as fever, headaches, muscle pain, anemia, skin rashes, and ulcers. Importantly, sandflies are species-specific in their disease transmission. Determining the gender and species of sandflies typically involves examining their morphology and internal anatomy using established identification keys. However, this process requires expert knowledge and is labor-intensive, time-consuming, and prone to misidentification. In this paper, we develop a highly accurate and efficient convolutional network model that utilizes pharyngeal and genital images of sandfly samples to classify the sex and species of three sandfly species (i.e., Phlebotomus sergenti, Ph. alexandri, and Ph. papatasi). A detailed evaluation of the model’s structure and classification performance was conducted using multiple metrics. The results demonstrate an excellent sex-species classification accuracy exceeding 95%. Hence, it is possible to develop automated artificial intelligence-based systems that serve the entomology community at large and specialized professionals. Full article

(This article belongs to the Section Animal System and Management)

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19 pages, 1214 KiB

Open AccessFeature PaperArticle

Effects of Tuna By-Product Meal on Growth, Whole-Body Mercury, Phosphorus Load, and Plasma Chemistry in Juvenile Greater Amberjack Seriola dumerili

by Amal Biswas, Shota Shirakawa, Satoshi Okimura, Tomoki Honryo and Hideki Tanaka

Animals 2024, 14(24), 3711; https://doi.org/10.3390/ani14243711 - 23 Dec 2024

Viewed by 449

Abstract

The suitability of tuna by-product meal (TBM) as a replacement for fish meal (FM) in the diet of juvenile greater amberjack Seriola dumerili was investigated for its effect on growth, mercury (Hg) levels in the fish body, phosphorus (P) load, and plasma chemistry. [...] Read more.

The suitability of tuna by-product meal (TBM) as a replacement for fish meal (FM) in the diet of juvenile greater amberjack Seriola dumerili was investigated for its effect on growth, mercury (Hg) levels in the fish body, phosphorus (P) load, and plasma chemistry. FM was the main protein source in the control diet (C), and it was replaced by TBM at 25%, 50%, 75%, and 100% to formulate diets TM25, TM50, TM75, and TM100, respectively. The experiment was performed in triplicate, stocking 30 juveniles (mean weight: approximately 6.7 g) in each 500 L tank. After a 42 d rearing period, there were no significant differences in growth, nutrient digestibility, or productive value between diets C and TM25. However, a quadratic polynomial regression analysis suggested that the optimal dietary level of TBM against final weight was 14.5%. A significant linear correlation was observed between Hg in the diet and fish whole body, but the level was far lower, posing health risks to humans. Owing to the lower P intake of fish fed with the TBM-based diets, their P load was significantly reduced compared to the control group. Plasma levels of total protein, total cholesterol, and albumin in the TM75 and TM100 groups were significantly lower than those in the C, TM25, and TM50 groups. These results suggest that 14.5% of FM protein can be replaced by TBM protein in the diet of juvenile greater amberjack and that a significant reduction in P load can be achieved with a TBM-based diet. Full article

(This article belongs to the Special Issue Advances in Functional Diets and Culture Conditions in Fish: Applications in Aquaculture)

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14 pages, 8023 KiB

Open AccessArticle

Effects of Oregano Essential Oil and/or Yeast Cultures on the Rumen Microbiota of Crossbred Simmental Calves

by Ting Liu, Zhihao Luo, Tao Zhang, Huan Chen, Xuejiao Yi, Jiang Hu, Bingang Shi, Yuxi An, Changze Cui and Xiangyan Wang

Animals 2024, 14(24), 3710; https://doi.org/10.3390/ani14243710 - 23 Dec 2024

Viewed by 459

Abstract

This study hypothesized that combining oregano essential oil (OEO) and yeast cultures (YCs) would modulate rumen microbiota to promote gastrointestinal homeostasis and function. Twenty-four newborn, healthy, disease-free, crossbred Simmental male calves (birth weight ≥ 35 kg) were assigned to [...] Read more.

This study hypothesized that combining oregano essential oil (OEO) and yeast cultures (YCs) would modulate rumen microbiota to promote gastrointestinal homeostasis and function. Twenty-four newborn, healthy, disease-free, crossbred Simmental male calves (birth weight ≥ 35 kg) were assigned to one of four treatments based on birth data. Treatments were as follows: (1) Control (CON), calves fed calf starter without additives; (2) OEO, calves fed calf starter containing 60 mg/kg body weight (BW) of OEO per day; (3) YCs, calves fed calf starter containing 45 mg/kg BW of YC per day; and (4) MIX, calves fed calf starter with OEO (60 mg/kg, BW) and YC (45 mg/kg, BW) combination. The experimental period lasted 70 days. Rumen fluid was collected on the final day, and 16S rRNA sequencing was performed to assess alterations in rumen microbiota. Calves fed MIX exhibited significantly greater microbial richness, species diversity, and lineage diversity (p < 0.05) compared with calves in the other groups. MIX-fed calves also showed changes (p < 0.05) in the relative abundance of certain rumen species, identified as through LEfSe analysis (LDA > 4, p < 0.05). These biomarkers included f_Rikenellaceae, g_Rikenellaceae_RC9_gut_group, g_Erysipelotrichaceae_UCG-002, c_Saccharimonadia, o_Saccharimonadales, f_Saccharimonadaceae, and g_Candidatus_Saccharimonas. Pathways enriched (p < 0.05) in MIX-fed calves involved nucleotide metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, terpenoids and polyketides metabolism, antimicrobial drug resistance, xenobiotic biodegradation and metabolism, antineoplastic drug resistance, and excretory system pathways. In conclusion, this study demonstrates that the OEO and YC combination enhances rumen microbial community modulation in calves more effectively than OEO or YCs fed individually or with the control diet. Full article

(This article belongs to the Section Cattle)

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16 pages, 3526 KiB

Open AccessCommunication

Genome-Wide Microsatellites in Acanthopagrus latus: Development, Distribution, Characterization, and Polymorphism

by Chao Peng, Congqiang Luo, Guangqing Xiang, Jiezhen Huang, Liye Shao, Haihong Huang and Sigang Fan

Animals 2024, 14(24), 3709; https://doi.org/10.3390/ani14243709 - 23 Dec 2024

Viewed by 409

Abstract

The yellowfin seabream (Acanthopagrus latus) is an economically important commercial mariculture fish in China and Southeast Asia. Only a few simple sequence repeats (SSRs) of A. latus have been isolated and reported, which has hindered breeding progress. A total of 318,862 [...] Read more.

The yellowfin seabream (Acanthopagrus latus) is an economically important commercial mariculture fish in China and Southeast Asia. Only a few simple sequence repeats (SSRs) of A. latus have been isolated and reported, which has hindered breeding progress. A total of 318,862 SSRs were isolated and characterized from the A. latus genome in this study. All SSRs were 9,069,670 bp in length, accounting for 1.32% of the genome. The density and frequency of SSRs were 468.40 loci/Mb and 13,323.19 bp/Mb, respectively. The major SSRs were dinucleotides (accounting for 76.92%), followed by trinucleotides (15.75%). The most abundant SSR motif was (AC)n (168,390, accounting for 53%), with the highest frequency (245.78 loci/Mb) and density (7304.18 bp/Mb). Most SSRs were located in non-coding regions, such as intergenic regions (34.54%) and introns (56.91%). SSR-containing exons were distributed into 51 gene ontology (GO) terms and significantly enriched in immunity- and growth-related pathways. A total of 217,791 SSR markers were successfully designed. Nine SSR markers were amplified in 29 A. latus individuals, and eight of them possess high polymorphism. The cross-species transferability of 33 out of the 37 tested loci were successfully amplified in Acanthopagrus schlegelii. These results lay the foundation for the molecular marker-assisted breeding and genetic information assessment of A. latus. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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15 pages, 564 KiB

Open AccessFeature PaperArticle

Exploring Relationship Between Immunocompetence, HPA Axis Functioning and Performances of Preweaning Dairy Calves

by Alessio Cotticelli, Giovanna Bifulco, Matilde Giombolini, Isabella Pividori, Alfio Calanni Macchio, Gianluca Neglia, Mirco Corazzin, Alberto Prandi and Tanja Peric

Animals 2024, 14(24), 3708; https://doi.org/10.3390/ani14243708 - 23 Dec 2024

Viewed by 437

Abstract

Cortisol and DHEA(S) are markers of allostatic load and resilience; as such, they may be added to the definition of performance measures alongside immunocompetence. We aimed at studying the potential carryover effects of management practices on growth performances and the interrelationship between the [...] Read more.

Cortisol and DHEA(S) are markers of allostatic load and resilience; as such, they may be added to the definition of performance measures alongside immunocompetence. We aimed at studying the potential carryover effects of management practices on growth performances and the interrelationship between the hypothalamic–pituitary–adrenal (HPA) axis, immunocompetence and performances during the preweaning period in dairy calves. This study consisted of first a retrospective cohort study and second a prospective study. Calves had their growth performances and disease occurrence recorded, and their hair steroids (T1 at 20.03 ± 0.39 and T2 at 50.83 ± 0.41 days from birth) and plasmatic and colostral immunoglobulin G (IgG) was measured by immunoassays. The occurrence of preweaning diseases and the duration of individual housing negatively correlated to body weight (BW) at 60 days and the hair cortisol-to-DHEA(S) ratio (HC/HDHEA(S)) (T2) was negatively correlated to the daily weight gain (DWG) (−0.36; p < 0.05), plasmatic IgG (plaIgG) and the apparent efficiency of absorption (AEA) (−0.41; p < 0.01). Our results confirmed that the occurrence of diseases during the preweaning period and individual housing duration are pivotal for calves’ growth. This prospective study highlighted a detrimental effect of allostatic load on immunocompetence and described a link between resilience and growth performances. Full article

(This article belongs to the Section Animal Physiology)

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4 pages, 463 KiB

Open AccessEditorial

Factors Affecting Milk Productivity, Milk Quality and Dairy Cow Health

by Michał Bednarski and Robert Kupczyński

Animals 2024, 14(24), 3707; https://doi.org/10.3390/ani14243707 - 23 Dec 2024

Viewed by 569

Abstract

Milk and dairy products are considered important sources of nutrients in human nutrition due to their content of high-quality protein, minerals, vitamins, and energy [...] Full article

(This article belongs to the Special Issue Factors Affecting Milk Productivity, Milk Quality, and Dairy Cow Health)

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16 pages, 12838 KiB

Open AccessArticle

Evaluation of the Effect of Adipokinetic Hormone/Corazonin-Related Peptide (ACP) on Ovarian Development in the Mud Crab, Scylla paramamosain

by Wenting Tan, Yiwei Tang, Fang Liu, Li Lu, An Liu and Haihui Ye

Animals 2024, 14(24), 3706; https://doi.org/10.3390/ani14243706 - 23 Dec 2024

Viewed by 480

Abstract

In this study, we identified Sp-ACP and its putative receptor from the mud crab S. paramamosain and explored its potential role in ovarian development. RT-PCR results suggested Sp-ACP was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while [...] Read more.

In this study, we identified Sp-ACP and its putative receptor from the mud crab S. paramamosain and explored its potential role in ovarian development. RT-PCR results suggested Sp-ACP was extensively expressed in nervous tissues, the ovary, the middle gut, and the Y-organ, while Sp-ACPR was highly expressed in the ovary. The expression level of Sp-ACP in the ovary, eyestalk, and cerebral ganglia gradually increased during ovarian development, whereas its receptor exhibited an opposite expression pattern in the ovary. Immunofluorescence revealed that ACP was specifically localized in the follicle cells of the ovary. In vitro experiments showed that the expression of vitellogenin receptor (Sp-VgR) in the ovary was significantly increased by 4 and 6 h incubation of Sp-ACP (10 nM). In addition, 12 h injection of Sp-ACP significantly induced the levels of Sp-Vg in the hepatopancreas and Sp-VgR in the ovary, and hemolymph 17β-estradiol titer. Finally, it demonstrated that prolonged injection of Sp-ACP significantly increased the level of Vg and VgR expression, hemolymph 17β-estradiol titer, GSI, and the oocyte diameter. In conclusion, our results suggested that ACP is involved in the regulation of ovarian development of S. paramamosain, likely by inducing hepatopancreas Sp-Vg expression through estradiol and promoting the uptake of Vg by oocytes. Full article

(This article belongs to the Section Animal Reproduction)

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14 pages, 1152 KiB

Open AccessArticle

An Investigation of the Saccharides Profile and Metabolic Gene Expression in Muskrat Scented Glands in Different Secretion Seasons

by Juntong Zhou, Defu Hu, Nuannuan Feng, Shuqiang Liu and Junqing Li

Animals 2024, 14(24), 3705; https://doi.org/10.3390/ani14243705 - 22 Dec 2024

Viewed by 563

Abstract

The adult male muskrat has a pair of scented glands, which show clear seasonal changes in their developmental status between the secretion season and non-secretion season. During the secretion season, the scented glands are much larger than in the non-secretion season, with the [...] Read more.

The adult male muskrat has a pair of scented glands, which show clear seasonal changes in their developmental status between the secretion season and non-secretion season. During the secretion season, the scented glands are much larger than in the non-secretion season, with the metabolism of glandular cells increasing and a large amount of musk being produced. In this work, the blood, musk, and scented gland tissue were collected from three healthy adult male muskrats during secretion season (September). And the blood and scented gland tissue from another three healthy adult male muskrats during the non-secretion season (November) were also sampled. The saccharides from blood and musk were detected by liquid chromatography–mass spectrometry (LC-MS), indicating the saccharides are concentrated in the scented glands during the secretion season. What is more, transcriptome analysis was employed to investigate the expression patterns of saccharides’ pathways, suggesting some saccharides’ metabolism-related genes undergo significant seasonal changes. Above all, scented gland saccharides’ metabolism displays seasonal differences, and the enhancement in saccharides’ metabolic activity during the secretion phase maintains glandular proliferation and secretion function. Full article

(This article belongs to the Section Mammals)

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12 pages, 1600 KiB

Open AccessArticle

Predicting Tuberculosis Risk in Cattle, Buffaloes, Sheep, and Goats in China Based on Air Pollutants and Meteorological Factors

by Le Xu, Suya Li, Hong Li, Haoju Pan, Shiyuan Li, Yingxue Yang, Yuqing Jiao, Feng Lan, Si Chen, Qiaoling Chen, Li Du, Churiga Man, Fengyang Wang and Hongyan Gao

Animals 2024, 14(24), 3704; https://doi.org/10.3390/ani14243704 - 22 Dec 2024

Viewed by 725

Abstract

Tuberculosis is a zoonotic chronic respiratory infectious disease caused by the Mycobacterium tuberculosis complex. The outbreak and epidemic of tuberculosis can seriously threaten human and veterinary health. To investigate the effects of environmental factors on tuberculosis in domestic ruminants, we collected data regarding [...] Read more.

Tuberculosis is a zoonotic chronic respiratory infectious disease caused by the Mycobacterium tuberculosis complex. The outbreak and epidemic of tuberculosis can seriously threaten human and veterinary health. To investigate the effects of environmental factors on tuberculosis in domestic ruminants, we collected data regarding the prevalence of tuberculosis in cattle, buffaloes, sheep, and goats in China (1956–2024) from publicly published literature and available databases. We identified the key risk factors among six major air pollutants and 19 bioclimatic variables; simulated the risk distribution of tuberculosis in cattle, buffaloes, sheep, and goats in China using the maximum entropy ecological niche model; and evaluated the effects of environmental factors. The area under the curve of the model was 0.873 (95% confidence interval, 0.851–0.895). The risk factors that most significantly influenced the prevalence of tuberculosis were the nitrogen dioxide (NO₂) level, mean temperature of the coldest quarter, cattle distribution density, sheep distribution density, ozone (O₃) level, and precipitation of the driest month. The predicted map of tuberculosis risk in cattle, buffaloes, sheep, and goats indicated that the high-risk regions were mainly distributed in South, North, East, and Northwest China. Improved surveillance is needed in these high-risk areas, and early preventive measures must be implemented based on the risk factors identified to reduce the future prevalence of tuberculosis in cattle, buffaloes, sheep, and goats. Full article

(This article belongs to the Section Veterinary Clinical Studies)

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23 pages, 748 KiB

Open AccessFeature PaperArticle

Myotonia Congenita in Australian Merino Sheep with a Missense Variant in CLCN1

by Leah K. Manning, Katie L. M. Eager, Cali E. Willet, Shaun Slattery, Justine H. McNally, Zoe B. Spiers, Mark Hazelton, Georgina Child, Rick Duggan, Brendon A. O’Rourke and Imke Tammen

Animals 2024, 14(24), 3703; https://doi.org/10.3390/ani14243703 - 22 Dec 2024

Viewed by 752

Abstract

Myotonia congenita is a hereditary, non-dystrophic skeletal muscle disorder associated with muscle stiffness due to delayed muscle relaxation after contraction. We review myotonia congenita in domesticated animals and humans and investigated suspected myotonia congenita in a flock of Merino sheep in Australia. In [...] Read more.

Myotonia congenita is a hereditary, non-dystrophic skeletal muscle disorder associated with muscle stiffness due to delayed muscle relaxation after contraction. We review myotonia congenita in domesticated animals and humans and investigated suspected myotonia congenita in a flock of Merino sheep in Australia. In 2020, a property in New South Wales reported a four-year history of lambs that would fall on disturbance before rapidly recovering, with 13 affected sheep identified in 2020. Episodes were associated with a short period of tetanic spasms and a stiff gait upon rising. Lambs were otherwise normal between episodes, although over time, lost body condition and occasionally died from misadventure. An inherited condition was considered from limited pedigree information and a preliminary diagnosis of myotonia congenita was made based on clinical presentation. Biochemistry from four sheep found variable, but typically mild increases in creatine kinase (CK) and aspartate aminotransferase (AST). Modified electromyography on six affected sheep found irregular electrical activity within the muscle. For four sheep, there were no consistent significant abnormalities on post mortem examination and histopathology—typical for this condition. A review of the Online Mendelian Inheritance in Man (OMIM) and Online Mendelian Inheritance in Animals (OMIA) databases was conducted to summarise information about myotonia congenita in humans and eight non-human species of animals. Comparing the characteristic clinical presentation, pathology and electromyography data of affected Merino sheep to similar conditions in other species assisted the identification of likely candidate genes. Whole genome sequencing of two affected lambs detected a missense variant in CLCN1 (NC_056057.1:g.107930611C>T; XM_004008136.5:c.844C>T; XP_004008185.4:p.(P282S)), with a predicted deleterious effect on protein function. An SNP genotyping assay was developed, and the variant segregated with the disease in 12 affected sheep and obligate carrier rams under an assumed recessive mode of inheritance. Identifying a likely causal variant and developing a diagnostic test allows screening of suspected affected or carrier Merino sheep for early intervention to reduce propagation of the variant within flocks. Full article

(This article belongs to the Section Small Ruminants)

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15 pages, 4045 KiB

Open AccessArticle

Mulberry Branch Extracts Enhance the Antioxidant Capacity of Broiler Breast Muscle by Activating the Nrf2 and Cytochrome P450 Signaling Pathway

by Xiang Shi, Wei Qian, Xinlan Wei, Xiaoqing Qin, Jinyan Han, Chao Su and Lijun Bao

Animals 2024, 14(24), 3702; https://doi.org/10.3390/ani14243702 - 22 Dec 2024

Viewed by 545

Abstract

Mulberry branch extracts (MBEs) have garnered significant attention as natural feed additives and antioxidants; however, their antioxidant properties in meat post-slaughter and their influence on muscle-related metabolic processes remain largely unexplored. Herein, we evaluated the effects of MBEs on the antioxidant capacity and [...] Read more.

Mulberry branch extracts (MBEs) have garnered significant attention as natural feed additives and antioxidants; however, their antioxidant properties in meat post-slaughter and their influence on muscle-related metabolic processes remain largely unexplored. Herein, we evaluated the effects of MBEs on the antioxidant capacity and metabolic processes of breast muscle in yellow-feather broilers by adding 0 g/kg, 1.5 g/kg, 3.0 g/kg, and 4.5 g/kg of MBEs to their diets. The results demonstrate that MBEs enhanced the activity of antioxidant enzymes in muscle tissue. Specifically, a real-time quantitative PCR analysis revealed that MBEs increased the expression of antioxidant enzyme genes in a dose-dependent manner, activated the Nrf2 signaling pathway, and upregulated the expression of the Nrf2 gene and its downstream targets at doses of up to 3.0 g/kg. Furthermore, the results of widely targeted metabolomics indicate that the dietary supplementation of MBEs changed the amino acid profile of the muscle, increasing the levels of amino acids and small peptides that contribute to antioxidant properties while reducing the contents of oxidized lipids and carnitine (C5:1) and partially reducing the content of lysophosphatidylcholine (LPC). Notably, at doses of up to 3 g/kg, the levels of five signature bile acids increased in correlation with the added dose. A KEGG analysis indicated that the differential metabolites were predominantly enriched in the metabolism of xenobiotics by cytochrome P450, suggesting that the function of MBEs may be associated with the expression of P450 enzymes. In summary, this study demonstrates that MBEs are effective, safe, and natural antioxidants, offering a viable solution to mitigating oxidative stress in the yellow-feather broiler farming industry and even in livestock farming. Full article

(This article belongs to the Special Issue Sustainable Poultry Production: Physiology and Nutrition (Second Edition))

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15 pages, 1174 KiB

Open AccessArticle

Residue Elimination Patterns and Determination of the Withdrawal Times of Seven Antibiotics in Eggs of Taihang Chickens

by Huan Chen, Xiajun Wu, Shasha Cui, Yandong Li, Yingli Mu, Jinduo Gao, Huage Liu and Juxiang Liu

Animals 2024, 14(24), 3701; https://doi.org/10.3390/ani14243701 - 22 Dec 2024

Viewed by 388

Abstract

The objective of this study was to examine the residue elimination patterns of seven antibiotics in the eggs of Taihang chickens under free-range conditions and develop suitable withdrawal times (WDTs). A total of 240 healthy Taihang chickens, aged 180 days, were randomly divided [...] Read more.

The objective of this study was to examine the residue elimination patterns of seven antibiotics in the eggs of Taihang chickens under free-range conditions and develop suitable withdrawal times (WDTs). A total of 240 healthy Taihang chickens, aged 180 days, were randomly divided into eight groups of 30 birds each. The first seven groups were administered oxytetracycline, chlortetracycline, erythromycin, tylosin, tylvalosin, lincomycin, and tiamulin, respectively, in accordance with the maximum dosages and longest durations of treatment recommended by the Veterinary Pharmacopoeia of the People’s Republic of China. Group 8 served as the control group, and the test period continued until no drug residue could be detected. The results demonstrate that the residues of oxytetracycline, chlortetracycline, tylosin, tylvalosin, and tiamulin throughout the test period were below the maximum residue limits (MRLs) outlined in GB 31650-2019, “National food safety standard-Maximum residue limits for veterinary drugs in foods”. The egg yolk was identified as the target tissue for estimating the withdrawal time periods (WDTs) of these seven drugs. It was thus concluded that the recommended withdrawal time for tiamulin should be 0 days. However, the WDTs of oxytetracycline, chlortetracycline, tylosin, tylvalosin, were 2.8, 0.3, 2.4, and 7.4, respectively, when the upper limit of the 95% confidence interval was found to be lower than the MRLs. It was thus determined that the recommended WDTs for oxytetracycline, chlortetracycline, tylosin, and tylvalosin should be 3, 1, 3, and 8 days, respectively. In contrast, erythromycin and lincomycin exhibited concentrations below the permitted MRLs on days 7 and 8, respectively. However, the upper limits of the 95% confidence intervals for erythromycin and lincomycin were found to be below the MRLs when the WDTs were 11 and 8.9, respectively. This suggests that the recommended WDTs for these two antibiotics should be 11 days and 9 days. Full article

(This article belongs to the Section Poultry)

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12 pages, 3538 KiB

Open AccessArticle

Surveillance of Highly Pathogenic Avian Influenza Virus in Wild Canids from Pennsylvania, USA

by Kevin D. Niedringhaus, Taylor C. Chan, Ashley McDowell, Lauren Maxwell, Madison Stevens, Lane Potts, Erica Miller, Eman Anis, Kyle Van Why, Thomas Keller, David Stallknecht, Rebecca L. Poulson, Kaitlyn Bahrs and Justin D. Brown

Animals 2024, 14(24), 3700; https://doi.org/10.3390/ani14243700 - 22 Dec 2024

Viewed by 886

Abstract

The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b have caused mortality in a wide variety [...] Read more.

The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b have caused mortality in a wide variety of mammals, including members of the Canidae family, on multiple continents. Despite sporadic mortality events globally, the epidemiology and pathobiology of H5N1 HPAIV in wild canids remains poorly defined. During 2022–2024, 41 wild canid carcasses (diagnostic cases), including 23 red foxes and 18 gray foxes, were tested for the influenza A virus (IAV) via PCR, with five red fox kits testing positive (12%). Infected animals had variably severe encephalitis, pneumonia, and occasionally myocarditis associated with strong immunolabeling for IAV. Serum from 269 wild canids in Pennsylvania was tested for antibodies to IAV, including 133 samples collected prior to 2021 (pre-H5N1 HPAIV 2.3.4.4b introduction) and 136 collected after 2022 (post-H5N1 HPAIV 2.3.4.4b introduction). All samples collected prior to 2021 were seronegative for IAV. Two coyotes from 2024 were seropositive for IAV but were negative for antibodies to the H5 and N1 subtypes. Collectively, these data suggest that while sporadic H5N1 HPAIV infection and mortality can occur in wild canids, particularly juvenile red foxes, infection was limited in these outwardly healthy and opportunistically sampled animals. Future studies should utilize a risk-based approach to target sampling of wild canids at increased risk for H5N1 HPAIV infection, such as those around waterfowl habitats or spatially around wild bird or domestic animal outbreaks. Full article

(This article belongs to the Special Issue Emerging Infectious Diseases of Wildlife: A Threat for Biodiversity and Global Health)

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16 pages, 285 KiB

Open AccessArticle

Impact of Automation Level of Dairy Farms in Northern and Central Germany on Dairy Cattle Welfare

by Lianne Lavrijsen-Kromwijk, Susanne Demba, Ute Müller and Sandra Rose

Animals 2024, 14(24), 3699; https://doi.org/10.3390/ani14243699 - 21 Dec 2024

Viewed by 672

Abstract

An increasing number of automation technologies for dairy cattle farming, including automatic milking, feeding, manure removal and bedding, are now commercially available. The effects of these technologies on individual aspects of animal welfare have already been explored to some extent. However, as of [...] Read more.

An increasing number of automation technologies for dairy cattle farming, including automatic milking, feeding, manure removal and bedding, are now commercially available. The effects of these technologies on individual aspects of animal welfare have already been explored to some extent. However, as of now, there are no studies that analyze the impact of increasing farm automation through various combinations of these technologies. The objective of this study was to examine potential correlations between welfare indicators from the Welfare Quality^® Assessment protocol and dairy farms with varying degrees of automation. To achieve this, 32 trial farms in Northern and Central Germany were categorized into varying automation levels using a newly developed classification system. The Welfare Quality^® Assessment protocol was used to conduct welfare assessments on all participating farms. Using analysis of variance (ANOVA), overall welfare scores and individual measures from the protocol were compared across farms with differing automation levels. No significant differences were observed in overall welfare scores, suggesting that the impact of automation does not exceed other farm-related factors influencing animal wellbeing, such as housing environment or management methods. However, significant effects of milking, feeding, and bedding systems on the appropriate behavior of cattle were observed. Higher levels of automation had a positive impact on the human–animal relationship and led to positive emotional states. Moreover, farms with higher automation levels had significantly lower scores for the prevalence of severe lameness and dirtiness of lower legs. It could be concluded that a higher degree of automation could help to improve animal welfare on dairy farms. Full article

(This article belongs to the Special Issue Modern Livestock and Poultry Farming Technologies to Improve Comfort and Animal Welfare Levels)

16 pages, 5182 KiB

Open AccessArticle

Analysis of Growth Models in Galician × Nelore Crossbred Cattle in the First Year of Life

by Antonio Iglesias, Fernando Mata, Joaquim Lima Cerqueira, Alicja Kowalczyk, Jesús Cantalapiedra, José Ferreiro and José Araújo

Animals 2024, 14(24), 3698; https://doi.org/10.3390/ani14243698 - 21 Dec 2024

Viewed by 646

Abstract

The veal niche market is gaining momentum in Brazil. Locally known as ‘Vitelão’, veal refers to the meat from calves slaughtered up to 12 months of age. In this study, we assessed the Galician Blond × Nelore cross as a candidate to produce [...] Read more.

The veal niche market is gaining momentum in Brazil. Locally known as ‘Vitelão’, veal refers to the meat from calves slaughtered up to 12 months of age. In this study, we assessed the Galician Blond × Nelore cross as a candidate to produce veal. The aim of this study was to establish criteria for selecting 12-month-old calves suitable for slaughter. To find the best fit, we adjusted various growth models for calves up to 12 months of age. Once the best fit was determined, the selected growth model was then used to calculate the relative and instantaneous growth rates to evaluate the slaughtering potential at 12 months. Our study reveals that, under present conditions, the Logistic model is the best fit for characterizing and functionally analyzing growth from birth to 12 months of age in Galician Blond crosses with Nelore. Calves resulting from this cross experience rapid growth in their first 12 months of life, making them an excellent choice for producing high-quality veal while maintaining rusticity and adaptability to extreme environments. The results of this study could contribute to enhancing the growth management systems of Galician Blond and Nelore crosses in Brazilian grazing production systems. Additionally, they can be incorporated into genetic improvement programs as a tool for selecting animals with greater precocious growth without altering adult weight. Full article

(This article belongs to the Special Issue New Developments in Livestock Production Systems: Improving Meat Production)

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23 pages, 16678 KiB

Open AccessArticle

An Epidemiological Study on Salmonella in Tibetan Yaks from the Qinghai–Tibet Plateau Area in China

by Dengyu Li, Kaiqin Zhang, Xiaofeng Xue, Zhanchun Bai, La Yang, Jingjing Qi and Sizhu Suolang

Animals 2024, 14(24), 3697; https://doi.org/10.3390/ani14243697 - 21 Dec 2024

Viewed by 727

Abstract

Salmonella is an important foodborne pathogen that can cause a range of illnesses in humans; it has also been a key focus for monitoring in the field of public health, including gastroenteritis, sepsis, and arthritis, and can also cause a decline in egg [...] Read more.

Salmonella is an important foodborne pathogen that can cause a range of illnesses in humans; it has also been a key focus for monitoring in the field of public health, including gastroenteritis, sepsis, and arthritis, and can also cause a decline in egg production in poultry and diarrhea and abortion in livestock, leading to death in severe cases, resulting in huge economic losses. This study aimed to investigate the isolation rate, antimicrobial resistance, serotypes, and genetic diversity of Salmonella isolated from yak feces in various regions on the Qinghai–Tibet Plateau. A total of 1222 samples of yak dung were collected from major cities in the Qinghai–Tibet Plateau area, and the sensitivity of the isolated bacteria to 10 major classes of antibiotics was determined using the K-B paper disk diffusion method for drug susceptibility. Meanwhile, the serotypes of the isolated bacteria were analyzed using the plate agglutination test for serum antigens, and their carriage of drug resistance and virulence genes was determined using PCR and gel electrophoresis experiments. The isolated bacteria were also classified using MLST (Multi-Locus Sequence Typing). The overall isolation rate for Salmonella was 18.25% (223/1222), and the results of the antibiotic susceptibility tests showed that 98.65% (220/223) of the isolated bacteria were resistant to multiple antibiotics. In the 223 isolates of Salmonella, eight classes of 20 different resistance genes, 30 serotypes, and 15 different types of virulence genes were detected. The MLST analysis identified 45 distinct sequence types (STs), including five clonal complexes, of which ST34, ST11, and ST19 were the most common. These findings contribute valuable information about strain resources, genetic profiles, and typing data for Salmonella in the Qinghai–Tibet Plateau area, facilitating improved bacterial surveillance, identification, and control in yak populations. They also provide certain data supplements for animal Salmonella infections globally, filling research gaps. Full article

(This article belongs to the Section Cattle)

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12 pages, 2860 KiB

Open AccessFeature PaperArticle

The Immune Contexture in Canine Anal Sac Adenocarcinoma: Immunohistochemical Quantification of Tumor-Infiltrating Lymphocytes and Tumor-Associated Macrophages with Image Analysis

by Barbara Bacci, Barbara Brunetti, Cristiano Maino, Ginevra Martinoli, Nick J. Bacon and Giancarlo Avallone

Animals 2024, 14(24), 3696; https://doi.org/10.3390/ani14243696 - 20 Dec 2024

Viewed by 714

Abstract

Canine anal sac gland adenocarcinomas (ASACs) are locally aggressive and highly metastatic to regional lymph nodes. Tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) can be effective prognostic and predictive markers in numerous human neoplasms and are increasingly investigated in dogs. The aim of [...] Read more.

Canine anal sac gland adenocarcinomas (ASACs) are locally aggressive and highly metastatic to regional lymph nodes. Tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) can be effective prognostic and predictive markers in numerous human neoplasms and are increasingly investigated in dogs. The aim of this study was to characterize immune cells in canine ASACs and their relationship with tumor size, histologic metastatic status, and tumor clinical stage. Thirty ASACs with known tumor size, metastatic status, and clinical stage were immunolabeled for Iba1 (macrophages), CD20 (B cells), CD3 (T cells), and Foxp3 (regulatory T cells). With image analysis, two areas of 1 mm² were analyzed for each case at the tumor core (TC) and invasive margin (IM) and immune cells were counted. Eighteen patients had metastasis at the time of diagnosis, of which fifteen were nodal only, and three were both distant and nodal. The median tumor size was 32.5 mm (range 11–70). The clinical stage was I in five cases, II in seven cases, III in fifteen cases, and IV in three cases. T cells and macrophages were the most abundant immune cells in all tumors. Tumor size did not influence the number or type of infiltrating immune cells. By contrast, significantly higher numbers of TC T lymphocytes were found in patients without metastasis, while significantly higher numbers of TC macrophages were found in dogs with metastasis. Immune cell infiltrate did not differ according to clinical stage. The results indicate that the tumor immune microenvironment, specifically TILs and TAMs, contribute to tumor behavior and may influence metastatic potential; in particular, high CD3 infiltration may prevent tumor progression, while increased macrophage infiltration could promote it. Full article

(This article belongs to the Section Companion Animals)

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10 pages, 2657 KiB

Open AccessArticle

Ultrasonographic Measurement of Muscle and Subcutaneous Fat Thickness for the Objective Assessment of the Nutritional Status of Alpacas

by Sonja Franz, Melanie Andrich and Thomas Wittek

Animals 2024, 14(24), 3695; https://doi.org/10.3390/ani14243695 - 20 Dec 2024

Viewed by 456

Abstract

The aim of this study was to evaluate the ultrasonographic measurement of the subcutaneous fat and muscle layers at two different body locations as an objective tool with which to determine the nutritional status of alpacas. The results of ultrasonographic measurement were related [...] Read more.

The aim of this study was to evaluate the ultrasonographic measurement of the subcutaneous fat and muscle layers at two different body locations as an objective tool with which to determine the nutritional status of alpacas. The results of ultrasonographic measurement were related to body weight, determined by scale, and body condition score (BCS), determined by a scoring system. Differences between gender (female/male) and different reproductive statuses (castrated/intact males, pregnant, and early or non-pregnant females) were evaluated. In total, 160 alpacas were examined. Ultrasonography was performed first at the lumbar region, positioning a linear probe (8 MHz) between the second and third lumbar vertebrae perpendicular to the spinal column, and in the gluteal region, measuring the distance between skin, the subcutaneous fat layer, and the muscle layer. The results showed that the gender and pregnancy status of females had a significant influence on the ultrasonographic measurements at both localizations. Significant associations were found between body weight and the BCS. The BCS and ultrasonographic-measured soft-tissue thicknesses at both localizations were significantly associated for males and early or non-pregnant females. According to these results, ultrasonography can be recommended as an objective method with which to determine the nutritional status of alpacas. Full article

(This article belongs to the Section Animal System and Management)

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13 pages, 5956 KiB

Open AccessFeature PaperArticle

Predicting Body Weight from Birth to Old Age in Giant Pandas Using Machine Learning

by Xingyong Zhu, Jiaheng Li, Jie Gao, Jingchao Lan, Mingxi Li, Jue Deng, Wenpei Peng, Yongyou Feng, Bi Li, Huizhong Pang, Jiawen Liu, Jie Kou and Ye Wang

Animals 2024, 14(24), 3694; https://doi.org/10.3390/ani14243694 - 20 Dec 2024

Viewed by 552

Abstract

The giant panda (Ailuropoda melanoleuca) is one of the animals with the largest body weight differences between its birth and adult stages, where the newborn cub is 0.1% the size of its mother. The rapid growth of panda cubs has been [...] Read more.

The giant panda (Ailuropoda melanoleuca) is one of the animals with the largest body weight differences between its birth and adult stages, where the newborn cub is 0.1% the size of its mother. The rapid growth of panda cubs has been reported previously, but little is known about the growth pattern of their entire lifetime. In this study, we analyzed body weight records from 206 captive giant pandas collected between 2000 and 2022. The dataset comprised 26,340 measurements, including 12,314 records from 98 males and 14,026 from 108 females, with the oldest male and female being 32 and 37 years old, respectively. Utilizing machine learning, we predicted daily body weights throughout the pandas’ lifespan, allowing us to establish detailed daily normative body weight ranges from birth to advanced age. This study presents the first comprehensive analysis of lifetime body weight distribution in giant pandas, enhancing our understanding of their developmental biology and informing improved body weight management strategies for captive populations. Full article

(This article belongs to the Section Animal Physiology)

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23 pages, 6217 KiB

Open AccessFeature PaperArticle

Ancient DNA Reveals the Earliest Evidence of Sheep Flocks During the Late Fourth and Third Millennia BC in Southern Iberia

by Gabriel Anaya, Juan Manuel Garrido, José Antonio Riquelme, Rafael Mª. Martínez, Alberto Membrillo, José Antonio Caro, Ana Pajuelo, Adrián Ruiz, José C. Martín de la Cruz and Antonio Molina

Animals 2024, 14(24), 3693; https://doi.org/10.3390/ani14243693 - 20 Dec 2024

Viewed by 846

Abstract

The Spanish Merino is the most significant sheep breed globally due to its economic and cultural importance in human history. It has also had a substantial influence on the development of other Merino and Merino-derived breeds. Historical sources indicate that crossbreeding to produce [...] Read more.

The Spanish Merino is the most significant sheep breed globally due to its economic and cultural importance in human history. It has also had a substantial influence on the development of other Merino and Merino-derived breeds. Historical sources indicate that crossbreeding to produce finer, higher-quality wool was already taking place in the south of the Iberian Peninsula during the Roman era. This evidence suggests that individuals with a racial pattern very similar to that of the modern Merino may have already existed on the peninsula. The presence of the skeletal remains of these animals at various human settlements dated to the late fourth and third millennia BC could provide insights into the genomics of these ancestral sheep. This study analyses ancient DNA extracted from nine skeletal remains from three archaeological sites in Southern Iberia, dated to the third millennium BC. The samples were sequenced and aligned with the ovine genome. The genetic distances observed among the samples indicate a closer relationship between several animals from the Marinaleda (Seville) and Grañena Baja (Jaén) sites. The study of the slaughter/death age profiles identified at La Minilla (La Rambla, Córdoba) suggests an approach centred on meat exploitation, while the data from Marinaleda (Seville) and Grañena Baja (Jaén) indicate the potential exploitation of secondary resources. A review of the composition of these small ruminant herds could provide insights into the type of secondary resource exploitation that may have been prioritised. Our aim is to investigate the presence of distinct production systems, differentiating between those aimed primarily at meat use and those focused on secondary products. This is the first approach to exploring the genetic evidence for sheep livestock related to its productive use during this period and in this geographical area. Full article

(This article belongs to the Collection Genetic Diversity in Livestock and Companion Animals)

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18 pages, 870 KiB

Open AccessFeature PaperArticle

Specialized Feed-Additive Blends of Short- and Medium-Chain Fatty Acids Improve Sow and Pig Performance During Nursery and Post-Weaning Phase

by Sandra Villagómez-Estrada, Diego Melo-Durán, Sandra van Kuijk, José F. Pérez and David Solà-Oriol

Animals 2024, 14(24), 3692; https://doi.org/10.3390/ani14243692 - 20 Dec 2024

Viewed by 746

Abstract

The present study investigates the impact of supplementing diets with a synergistic blend of short- and medium-chain fatty acids (SCFAs-MCFAs) during the peripartum and lactation phases on early microbial colonization and the subsequent growth performance of newborn pigs. The experiment involved 72 sows [...] Read more.

The present study investigates the impact of supplementing diets with a synergistic blend of short- and medium-chain fatty acids (SCFAs-MCFAs) during the peripartum and lactation phases on early microbial colonization and the subsequent growth performance of newborn pigs. The experiment involved 72 sows and their litters, with a follow-up on 528 weaned pigs. Sows were fed either a control diet or a diet supplemented with SCFAs-MCFAs and the pigs were monitored for their growth performance and microbial populations. Subsequently, selected weaned pigs were allotted to an SCFAs-MCFAs diet according to the maternal diet. Results showed that SCFAs-MCFAs supplementation led to reduced backfat loss in sows and improved pig weight and uniformity at weaning (p < 0.05). Additionally, suckling pigs exhibited significant shifts in gut microbiota, including increased lactic acid bacteria and reduced Streptococcus suis populations (p < 0.05). Although there was no influence of maternal diet on pig growth after weaning, there was a modulation on bacterial populations at 7 and 35 days post-weaning. Pigs fed SCFAs-MCFAs demonstrated improved feed efficiency with notable reductions in E. coli and Streptococcus suis counts. The findings suggest that maternal dietary supplementation with SCFAs-MCFAs can positively influence both sow and pig performance, offering a potential strategy to enhance productivity and health in the commercial swine production. Full article

(This article belongs to the Section Pigs)

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18 pages, 2093 KiB

Open AccessArticle

Willing but Unable: Moral Distress and Burnout in Italian Veterinarians Working with Companion and Farm Animals

by Emanuela Prato-Previde, Barbara De Mori, Nicoletta Colombo and Annalisa Pelosi

Animals 2024, 14(24), 3691; https://doi.org/10.3390/ani14243691 - 20 Dec 2024

Viewed by 510

Abstract

Veterinarians face ethical challenges during their careers, but despite evidence of work-related stress and burnout in veterinarians, moral distress has been poorly investigated. Using an online survey, professional experience, moral distress, and burnout were investigated in 704 Italian veterinarians caring for companion animals, [...] Read more.

Veterinarians face ethical challenges during their careers, but despite evidence of work-related stress and burnout in veterinarians, moral distress has been poorly investigated. Using an online survey, professional experience, moral distress, and burnout were investigated in 704 Italian veterinarians caring for companion animals, farm animals, or both. The reliability of the moral distress questionnaire was optimal, and Factor analysis identified four factors for moral distress: I. Conflict with owner/farmer; II. Relationships with colleagues/superiors; III. Legal context/unsupportive Professional Institutions; IV. Unshared requests for euthanasia. Veterinarians reported rather low levels of moral distress but high levels of work-related stress/anxiety and a decline in empathy over time, mainly toward owners/farmers. More than 50% of veterinarians reported medium/high levels of burnout, and client-related burnout was significantly higher than animal-related burnout. Levels of work-related stress, moral distress, and burnout were higher in women, and experience exerted a protective effect. Moral distress was positively correlated with emotional exhaustion and depersonalization and, to a lesser extent, lower professional fulfillment. The relatively low level of moral distress that emerged in the study needs further investigation, especially considering the high levels of work-related stress and anxiety reported by veterinarians. Women’s greater sensitivity to moral distress and burnout should not be overlooked given the progressive feminization of the veterinary profession worldwide. Full article

(This article belongs to the Section Human-Animal Interactions, Animal Behaviour and Emotion)

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16 pages, 2095 KiB

Open AccessFeature PaperArticle

Fertility of the European Brown Hare Across Plain, Hill and Mountain Environments in the Italian Peninsula

by Pierangelo Freschi, Carlo Cosentino, Egidio Mallia and Valter Trocchi

Animals 2024, 14(24), 3690; https://doi.org/10.3390/ani14243690 - 20 Dec 2024

Viewed by 413

Abstract

This study examines the reproductive performance of the European brown hare in Italy, focusing on four hunting districts in Basilicata (A1, A2) and Emilia–Romagna (A3, A4) and using the number of endometrial scars as a fertility indicator. Fertility, measured as the number of [...] Read more.

This study examines the reproductive performance of the European brown hare in Italy, focusing on four hunting districts in Basilicata (A1, A2) and Emilia–Romagna (A3, A4) and using the number of endometrial scars as a fertility indicator. Fertility, measured as the number of scars during the entire reproductive period, was highest in A1 (11.50 ± 1.64) and A4 (11.43 ± 1.01), followed by A3 (9.56 ± 1.09) and A2 (6.08 ± 1.34). Significant differences were found when comparing the mountain area (A2) with A4 (p < 0.01) and with A1 and A3 (p < 0.05). Regarding the overall monthly number of scars per individual across the four study areas, the highest value (3.16 ± 0.18) was observed in May, followed by March (2.74 ± 0.18), April (2.67 ± 0.20), June (2.57 ± 0.20), February (2.53 ± 0.34), July (2.40 ± 0.22), and August (2.19 ± 0.31). A correlation analysis identified a weak yet statistically significant positive relationship (r = 0.532; p = 0.036) between the Kerner Oceanity Index (with values of 16.87, 15.00, 8.45, and 11.11 in A1, A2, A3, and A4, respectively) and the number of scars per pregnancy across the four study areas. Understanding species’ reproductive dynamics is vital for assessing habitat suitability and creating strategies to sustain hare populations and their ecosystem roles. Full article

(This article belongs to the Collection Recent Advance in Wildlife Conservation)

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10 pages, 967 KiB

Open AccessArticle

Saccharomyces Cerevisiae Diet Supplementation Affects Nutrient Digestibility and Milk and Mozzarella Cheese Yield in Dairy Buffalo Cows During the Transition

by Fabio Zicarelli, Remus Costantin Vianello, Isabella Masiello, Nadia Musco, Piera Iommelli, Metha Wanapat, Daria Lotito, Pietro Lombardi, Micaela Grossi, Federico Infascelli and Raffaella Tudisco

Animals 2024, 14(24), 3689; https://doi.org/10.3390/ani14243689 - 20 Dec 2024

Cited by 1 | Viewed by 404

Abstract

The aim of the trial was to evaluate the influence of diet supplementation with Saccharomyces cerevisiae on nutrient digestibility and milk and mozzarella cheese yield in buffalo cows during the transition period. Twenty buffalo cows in the last month of pregnancy were equally [...] Read more.

The aim of the trial was to evaluate the influence of diet supplementation with Saccharomyces cerevisiae on nutrient digestibility and milk and mozzarella cheese yield in buffalo cows during the transition period. Twenty buffalo cows in the last month of pregnancy were equally divided into two groups (control, C, and treated, T) homogeneous for parity and milk yield at the previous lactation. The diet of group T was supplemented with 100 g/head/day of a product containing Saccharomyces cerevisiae (Sc 47- CNCM I-4407) for 4 weeks before and after calving. Dry matter intake and the body condition score were not affected by the dietary treatment. In contrast, organic matter, protein, NDF, and ADF digestibility significantly (p < 0.01) increased. Group T showed a higher milk yield (kg 10.5 vs. 9.2, p < 0.05) but lower milk fat (g/kg 76.0 vs. 80.1, p < 0.05). The buffalo standard milk (FPCM = 740 kcal) yield was higher in the treated group (kg 16.8 vs. 15.0, p < 0.05), whereas the mozzarella cheese yield was not affected by the treatment (p > 0.05). These findings indicate that supplementation of the diet of buffaloes with Saccharomyces cerevisiae during the transition period results in notable enhancements in nutrient digestibility and milk yield. These outcomes are worthy of further investigation. Full article

(This article belongs to the Special Issue Buffalo Farming as a Tool for Sustainability)

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12 pages, 316 KiB

Open AccessArticle

Additives in Supplements for Grazing Beef Cattle

by Karine R. S. Naves, Kamila. A. K. Moraes, Lorrayne O. da Cunha, Natasha B. Petrenko, Juliana C. Ortelam, Jarliane N. Sousa, Caroline F. Covatti, Dener Nunes, Carla S. Chaves, Flávio L. de Menezes, André S. de Oliveira and Eduardo H. B. K. Moraes

Animals 2024, 14(24), 3688; https://doi.org/10.3390/ani14243688 - 20 Dec 2024

Viewed by 620

Abstract

Pasture-based beef cattle production systems aim to maximize the interaction between forage and supplements to increase activity. In addition, supplementation and the use of food additives help optimize production efficiency and improve the use of additional nutrients. The aim of this study was [...] Read more.

Pasture-based beef cattle production systems aim to maximize the interaction between forage and supplements to increase activity. In addition, supplementation and the use of food additives help optimize production efficiency and improve the use of additional nutrients. The aim of this study was to evaluate the effects of the use of additives in protein–energy supplements (PESs) on the intake, digestibility, and ruminal parameters of beef cattle raised on pasture in the rearing phase. Five male cattle were allocated to a 5 × 5 Latin square design, with five experimental periods and five treatments. The animals were kept under continuous grazing in individual paddocks of marandu palisadegrass (Urochloa brizantha cv. Marandu). The treatments consisted of a mineral mixture (MM) (0.080 kg/animal/day), PES (4.0 kg/animal/day), PES with monensin sodium (120 mg/animal/day), PES with functional oil (2500 mg/animal/day) and PES with active yeast (4000 mg/animal/day). PESs increased the total dry matter intake (DMI) (kg/day) and the DMI in terms of % body weight (%BW) and increased the intake of crude protein (CP) and the dry matter intake of digestible organic matter (DOM). The use of additives did not affect nutrient intake or digestibility. Compared with MM, the PES increased the concentrations of ruminal ammonia nitrogen and serum urea nitrogen. In addition, there was an increase in fecal nitrogen, retained nitrogen, microbial nitrogen synthesis and microbial efficiency. The use of monensin sodium, functional oil and active yeast in protein–energy supplements for beef cattle in pastures did not affect the nutritional parameters under the conditions observed in this study. Full article

(This article belongs to the Section Cattle)

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Animals, Volume 14, Issue 24 (December-2 2024) – 166 articles

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