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Microorganisms, Volume 13, Issue 4

2025 April - 257 articles

Cover Story: This comprehensive review examines the pressing global health challenge of multidrug-resistant tuberculosis (MDR-TB) and explores cutting-edge strategies to overcome treatment limitations. The authors delve into the molecular basis of drug resistance in Mycobacterium tuberculosis while highlighting promising advances in nanomedicine, targeted drug delivery systems, and host-directed therapies. The review emphasizes the critical importance of multidisciplinary approaches that combine novel drug discovery, advanced delivery systems, and immunomodulation to address the complexities of MDR-TB treatment. With its focus on personalized medicine and innovative therapeutic strategies, this timely analysis provides valuable insights for researchers, clinicians, and policymakers working to develop effective, accessible, and affordable treatments for this persistent public health threat. View this paper
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Articles (257)

  • Article
  • Open Access
1 Citations
1,565 Views
15 Pages

Gardnerella vaginalis (GV), an opportunistic pathogen excessively proliferated in vaginal dysbiosis, causes systemic inflammation including vaginitis, neuroinflammation, and osteitis. To understand its systemic inflammation-triggering factor, we puri...

  • Article
  • Open Access
2 Citations
1,497 Views
16 Pages

Escherichia coli is a significant pathogen capable of inducing a variety of infections in both human and animal hosts. Despite its clinical significance, there is a lack of longitudinal research aimed at elucidating the genomic attributes that facili...

  • Article
  • Open Access
4 Citations
1,817 Views
19 Pages

Xanthomonas oryzae pv. oryzae (Xoo) is a biotrophic bacterial pathogen, which causes devastating bacterial blight disease worldwide. In this study, we thoroughly investigated the antimicrobial effect of the plant-derived extract chelerythrine against...

  • Review
  • Open Access
39 Citations
8,080 Views
18 Pages

Silver Nanoparticles and Antibiotics: A Promising Synergistic Approach to Multidrug-Resistant Infections

  • Eudald Casals,
  • Muriel F. Gusta,
  • Neus Bastus,
  • Jordi Rello and
  • Victor Puntes

The escalating threat of antibiotic resistance demands innovative strategies against multidrug-resistant (MDR) microorganisms, particularly in hospital settings where such infections represent a major global health challenge. Since the rapid growth o...

  • Review
  • Open Access
5 Citations
5,462 Views
24 Pages

Environmental Risk Factors Contributing to the Spread of Antibiotic Resistance in West Africa

  • Adenike Adenaya,
  • Adedapo Adedayo Adeniran,
  • Chidera Linus Ugwuoke,
  • Kaosara Saliu,
  • Mariam Adewumi Raji,
  • Amartya Rakshit,
  • Mariana Ribas-Ribas and
  • Martin Könneke

Antibiotic resistance is a well-documented global health challenge that disproportionately impacts low- and middle-income countries. In 2019, the number of deaths attributed to and associated with antibiotic resistance in Western Sub-Saharan Africa w...

  • Systematic Review
  • Open Access
2,500 Views
23 Pages

How the Built Environment Shapes Children’s Microbiome: A Systematic Review

  • Rozalynne Samira,
  • Muntazar Monsur and
  • Nazia Afrin Trina

This systematic review aims to synthesize key empirical findings to understand how various elements of the built environment influence the microbiome concerning children’s health and well-being. A comprehensive literature search was conducted a...

  • Article
  • Open Access
8 Citations
3,327 Views
22 Pages

Clinical success in treating complicated urinary tract infections (cUTIs) depends on accurate pathogen detection, given the common occurrence of polymicrobial infections and antimicrobial resistance. This multicenter, randomized, investigator-blinded...

  • Review
  • Open Access
7 Citations
5,555 Views
24 Pages

The Microbiota–Human Health Axis

  • Harrie Toms John,
  • Treesa Clare Thomas,
  • Ezenwa Collins Chukwuebuka,
  • Ali Bacar Ali,
  • Reggani Anass,
  • Yididiya Yilma Tefera,
  • Bency Babu,
  • Nicoleta Negrut,
  • Anca Ferician and
  • Paula Marian

Trillions of microorganisms play a pivotal role in maintaining health and preventing disease in humans. Their presence influences daily life, habits, energy levels, and pathologies. The present narrative review synthesized recent studies of microbial...

  • Article
  • Open Access
3 Citations
1,586 Views
22 Pages

Microbiome Migration from Soil to Leaves in Maize and Rice

  • Jiejia Ma,
  • Qianze Peng,
  • Silu Chen,
  • Zhuoxin Liu,
  • Weixing Zhang,
  • Chi Zhang,
  • Xiaohua Du,
  • Shue Sun,
  • Weiye Peng and
  • Yong Liu
  • + 4 authors

The interactions between plants and microbes are essential for enhancing crop productivity. However, the mechanisms underlying host-specific microbiome migration and functional assembly remain poorly understood. In this study, microbiome migration fr...

  • Article
  • Open Access
3 Citations
963 Views
12 Pages

New Findings on the Survival of Durusdinium glynnii Under Different Acclimation Methods to Low Salinities

  • Barbara de Cassia S. Brandão,
  • Jéssika L. de Abreu,
  • Deyvid Willame S. Oliveira,
  • Clarissa Vilela F. da Silva Campos,
  • Ingrid M. Thó de Aguiar,
  • Pedro R. de Sena,
  • Alfredo O. Gálvez and
  • Carlos Yure B. Oliveira

This study investigated the effects of salinity on the growth and cell morphotype of the coral-associated dinoflagellate Durusdinium glynnii under two acclimation strategies: abrupt saline shock (S5) and gradual reduction (S2). Results revealed optim...

  • Article
  • Open Access
2 Citations
1,266 Views
17 Pages

This study aimed to elucidate the therapeutic mechanisms of carbonate buffer mixture (CBM) in mitigating subacute rumen acidosis (SARA) by examining its effects on rumen pH, systemic inflammation, and rumen microbiota in a dairy goat model. Using a c...

  • Systematic Review
  • Open Access
1 Citations
3,012 Views
13 Pages

COVID-19 presents a wide range of symptoms, including gastrointestinal manifestations such as diarrhea, nausea, and abdominal pain. Lactobacillus acidophilus has been proposed as a potential adjunct therapy to alleviate these symptoms due to its prob...

  • Article
  • Open Access
1,438 Views
30 Pages

Through an integrated multi-omics analysis of rumen microbial communities, muscle transcriptomes, metabolic profiles, and liver metabolic profiles, this study systematically compared high- and low-performing lambs to elucidate their divergent effects...

  • Systematic Review
  • Open Access
6 Citations
1,699 Views
21 Pages

Evaluation of the Disinfection Efficacy of Er-YAG Laser Light on Single-Species Candida Biofilms: Systematic Review

  • Diana Dembicka-Mączka,
  • Magdalena Gryka-Deszczyńska,
  • Jacek Sitkiewicz,
  • Aleksander Makara,
  • Jakub Fiegler-Rudol and
  • Rafał Wiench

The relevance of the current study is to increase the resistance of fungal biofilms to traditional disinfection methods. The aim of the study was to determine how effectively Er:YAG laser light inhibits single-species Candida biofilms. The study invo...

  • Article
  • Open Access
4 Citations
1,342 Views
16 Pages

Analysis of Fecal Microbiome and Metabolome Changes in Goats When Consuming a Lower-Protein Diet with Varying Energy Levels

  • Hu Liu,
  • Anmiao Chen,
  • Wenji Wang,
  • Weishi Peng,
  • Kaiyu Mao,
  • Yuanting Yang,
  • Qun Wu,
  • Meng Zeng,
  • Ke Wang and
  • Hanlin Zhou
  • + 1 author

The objective of this study was to evaluate the effect of fecal bacterial community and metabolomics in goats when consuming a lower-protein diet with different energy levels. Eight healthy Leizhou goats, with 11 ± 0.78 kg of body weight, were...

  • Article
  • Open Access
1,933 Views
12 Pages

Bioprospecting of Marine Organisms: Exploring Antibacterial Activities in Aqueous and Organic Extracts

  • Vinícius Paulino Pinto Menezes,
  • Aldeni Moreira da Silva Filho,
  • Aline Jeferson Costa,
  • Elielton Nascimento,
  • Ulisses Santos Pinheiro,
  • Renata Pinheiro Chaves,
  • Alexandre Lopes Andrade,
  • Mayron Alves de Vasconcelos,
  • Edson Holanda Teixeira and
  • Rômulo Farias Carneiro
  • + 2 authors

This study evaluated the antibacterial activity of aqueous and organic extracts from 78 marine organisms, including seaweeds and sponges, collected along the coast of Ceará, Brazil. Extracts were obtained by maceration using distilled water an...

  • Review
  • Open Access
3 Citations
3,411 Views
30 Pages

Antibiotic Abuse in Ornamental Fish: An Overlooked Reservoir for Antibiotic Resistance

  • Chun Au-Yeung,
  • Yat-Lai Tsui,
  • Man-Hay Choi,
  • Ka-Wai Chan,
  • Sze-Nga Wong,
  • Yuk-Ki Ling,
  • Cheuk-Ming Lam,
  • Kit-Ling Lam and
  • Wing-Yin Mo

Ornamental fish represent a significant aquaculture sector with notable economic value, yet their contribution to antibiotic residues and resistance remains underrecognized. This review synthesizes evidence on widespread and often unregulated antibio...

  • Article
  • Open Access
1 Citations
1,097 Views
15 Pages

Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023

  • Fangxin Li,
  • Xin Zong,
  • Guosheng Chen,
  • Yu Zhang,
  • Qi Cao,
  • Lu Li,
  • Huanchun Chen,
  • Zhong Peng and
  • Chen Tan

Pasteurella multocida (PM), Glaesserella parasuis (GPS), and Actinobacillus pleuropneumoniae (APP) are among the species with the top five isolation rates on Chinese pig farms annually. To understand the antimicrobial susceptibility and genotypes of...

  • Article
  • Open Access
1,504 Views
14 Pages

Germination and Outgrowth of Bacillus subtilis Spores Deficient in BER and DisA Unveil Alternative Genetic Checkpoints

  • Alejandra Rangel-Mendoza,
  • Luz I. Valenzuela-García,
  • Eduardo A. Robleto and
  • Mario Pedraza-Reyes

During Bacillus subtilis spore germination/outgrowth, the rehydration of the spore core and activation of aerobic metabolism can generate reactive oxygen species (ROS)-promoted DNA lesions that are repaired via the base excision repair pathway (BER)....

  • Communication
  • Open Access
1,327 Views
15 Pages

Metataxonomics Characterization of Soil Microbiome Extraction Method Using Different Dispersant Solutions

  • David Madariaga-Troncoso,
  • Isaac Vargas,
  • Dorian Rojas-Villalta,
  • Michel Abanto and
  • Kattia Núñez-Montero

Soil health is essential for maintaining ecosystem balance, food security, and human well-being. Anthropogenic activities, such as climate change and excessive agrochemical use, have led to the degradation of soil ecosystems worldwide. Microbiome tra...

  • Review
  • Open Access
9 Citations
4,446 Views
25 Pages

Efficient utilization of lignin, a complex polymer in plant cell walls, is one of the key strategies for developing a green and sustainable bioeconomy. However, bioconversion of lignin poses a significant challenge due to its recalcitrant nature. Mic...

  • Article
  • Open Access
1 Citations
668 Views
16 Pages

To address freshwater lake blooms resulting from eutrophication, the application of quaternary ammonium compounds as algaecides serves as an effective emergency remediation strategy. Didecyl methylpropyl ammonium iodide (DMPAI) is a novel quaternary...

  • Article
  • Open Access
3 Citations
1,230 Views
18 Pages

This study was conducted to compare the effects of hydrolysable versus condensed tannins on growth performance, nutrient digestion, and rumen fermentation in goats. A total of 27 Liaoning cashmere goats with similar initial body weights were randomly...

  • Article
  • Open Access
3 Citations
1,510 Views
17 Pages

Spent mushroom substrate (SMS) is the residual biomass generated after harvesting the fruitbodies of edible fungi. It is produced in large quantities and contains abundant nutrients. Plant growth-promoting rhizobacteria (PGPR) are a group of plant-as...

  • Article
  • Open Access
3 Citations
2,563 Views
13 Pages

The Subgingival Microbial Composition in Health and Periodontitis with Different Probing Depths

  • Jingyan Wang,
  • Yiran Geng,
  • Jing Guo,
  • Jiahan Peng,
  • He Xu,
  • Bingqian Zhao,
  • Shiyan Huang,
  • Man Qin,
  • Wenbin Du and
  • Jing Tian

The differences in microbiota between periodontitis and health have been extensively studied; however, knowledge about how the microbiota shifts from shallow to deep periodontal pockets remains limited despite its clinical importance in disease progr...

  • Article
  • Open Access
1 Citations
1,041 Views
15 Pages

First Detection of Cryptosporidium Canis and Occurrence of Cryptosporidium spp. in Hospitalized Patients in Romania

  • Rodica Georgiana Dărăbuș,
  • Mirela Imre,
  • Gheorghe Dărăbuș,
  • Marius Stelian Ilie,
  • Alexander Tudor Olariu,
  • Diana Maria Dărăbuș,
  • Voichița Lăzureanu,
  • Ovidiu Roșca and
  • Tudor Rareș Olariu

The study aimed to identify Cryptosporidium infection in hospitalized patients in Western Romania. Stool samples were collected from 175 patients, both male and female, aged between 2 months and 88 years, residing in urban and rural areas. The identi...

  • Article
  • Open Access
1,220 Views
20 Pages

Candidatus Phytoplasma prunorum’ has been associated with severe disease in Prunus spp., which are commodities of economic importance in the USA. The introduction and establishment of ‘Ca. P. prunorum’ in the USA could result...

  • Article
  • Open Access
1 Citations
1,511 Views
13 Pages

Intra- and Post-Operative Bacteriological Surveys of Surgical Site in Horses: A Single-Centre Study

  • Anna Cerullo,
  • Matteo Riccardo Di Nicola,
  • Nicola Scilimati,
  • Alice Bertoletti,
  • Giuseppe Pollicino,
  • Barbara Moroni,
  • Marco Pepe,
  • Sara Nannarone,
  • Rodolfo Gialletti and
  • Fabrizio Passamonti

Bacterial contamination of the surgical site in horses is a major risk factor for the development of surgical site infections (SSIs), which increase morbidity, mortality, the hospitalisation period, antibiotic use, and management costs. While contami...

  • Review
  • Open Access
1 Citations
2,374 Views
22 Pages

The Strategy and Application of Gene Attenuation in Metabolic Engineering

  • Yahui Zhang,
  • Zhaoxia Jin,
  • Linxia Liu and
  • Dawei Zhang

Metabolic engineering has a wide range of applications, spanning key sectors such as energy, pharmaceuticals, agriculture, chemicals, and environmental sustainability. Its core focus is on precisely modulating metabolic pathways to achieve efficient,...

  • Article
  • Open Access
1 Citations
1,026 Views
19 Pages

Peanuts are widely cultivated across the world; however, peanut’s rhizobial community and the determinant factors of their composition are still to be elucidated. This study investigates the biogeography and determinant soil environmental facto...

  • Article
  • Open Access
2 Citations
1,530 Views
22 Pages

Wastewater Speaks: Evaluating SARS-CoV-2 Surveillance, Sampling Methods, and Seasonal Infection Trends on a University Campus

  • Shilpi Bhatia,
  • Tinyiko Nicole Maswanganye,
  • Olusola Jeje,
  • Danielle Winston,
  • Mehdi Lamssali,
  • Dongyang Deng,
  • Ivory Blakley,
  • Anthony A. Fodor and
  • Liesl Jeffers-Francis

Wastewater surveillance has emerged as a cost-effective and equitable approach for tracking the spread of SARS-CoV-2. In this study, we monitored the prevalence of SARS-CoV-2 on a university campus over three years (2021–2023) using wastewater-...

  • Article
  • Open Access
1,226 Views
17 Pages

Impact of Pre-Extraction Methods on Apple Blossom Microbiome Analysis

  • Nikhil N. Patel,
  • Jonathan R. Gaiero,
  • Muhammad Sulman,
  • Paul Moote,
  • Darlene Nesbitt,
  • Antonet M. Svircev and
  • Walid Ellouze

This study examines the effect of pre-extraction methods, namely, sonication, grinding, and lyophilization, and the use of peptide nucleic acid (PNA) blockers on the DNA recovery, diversity, and taxonomic resolution of bacterial and fungal communitie...

  • Article
  • Open Access
862 Views
22 Pages

Microbial Community Response and Assembly Process of Yellow Sand Matrix in a Desert Marginal Zone Under Morchella Cultivation

  • Cuicui Su,
  • Shengyin Zhang,
  • Yanfang Zhou,
  • Hao Tan,
  • Shuncun Zhang,
  • Tao Wang,
  • Zhaoyun Ding and
  • Jie Liao

In this study, we investigated the adaptation of yellow-sand-substrate Morchella cultivation in the desert fringe and its effect on soil physicochemical properties and microbial communities. The qPCR and high-throughput sequencing with null modeling...

  • Article
  • Open Access
2 Citations
1,416 Views
29 Pages

Development and Validation of Early Alert Model for Diabetes Mellitus–Tuberculosis Comorbidity

  • Zhaoyang Ye,
  • Guangliang Bai,
  • Ling Yang,
  • Li Zhuang,
  • Linsheng Li,
  • Yufeng Li,
  • Ruizi Ni,
  • Yajing An,
  • Liang Wang and
  • Wenping Gong

Diabetes mellitus (DM) and tuberculosis (TB) are two global health challenges that significantly impact population health, with DM increasing susceptibility to TB infections. However, early risk prediction methods for DM patients complicated with TB...

  • Article
  • Open Access
2 Citations
1,833 Views
14 Pages

Production and Characterization of Poly-γ-Glutamic Acid by Bacillus velezensis SDU

  • Guangyao Guo,
  • Han Wang,
  • Huiyuan Jia,
  • Haiping Ni,
  • Shouying Xu,
  • Cuiying Zhang,
  • Youming Zhang,
  • Yuxia Wu and
  • Qiang Tu

In this study, a Bacillus velezensis SDU strain capable of producing poly-γ-glutamate (γ-PGA) was newly identified from the rhizosphere soil of Baimiao taro. The strain is a glutamate-independent strain and can produce polyglutamic acid i...

  • Article
  • Open Access
1,473 Views
20 Pages

Hirudo verbana Microbiota Dynamics: A Key Factor in Hirudotherapy-Related Infections?

  • Djursun Karasartova,
  • Gonul Arslan-Akveran,
  • Sabiha Sensoz,
  • Kosta Y. Mumcuoglu and
  • Aysegul Taylan-Ozkan

The gastrointestinal microbiota of medicinal leeches is particularly interesting due to their blood-feeding habits, increasing medical use, and risk of pathogen transmission. Three groups of Hirudo verbana were used to study the leech microbiota: far...

  • Article
  • Open Access
1 Citations
1,154 Views
15 Pages

Effects of Seawater from Different Sea Areas on Abalone Gastrointestinal Microorganisms and Metabolites

  • Zhaolong Li,
  • Ling Ke,
  • Chenyu Huang,
  • Song Peng,
  • Mengshi Zhao,
  • Huini Wu and
  • Fengqiang Lin

Significant regional variations in seawater characteristics (temperature, salinity, pH, nutrients) exist across marine environments, yet their impacts on abalone gastrointestinal microbiota and metabolites remain underexplored. This study investigate...

  • Article
  • Open Access
2 Citations
1,773 Views
20 Pages

Members of Eubacteriaceae are involved in host health and diseases. Two Gram-stain-positive, strictly anaerobic, non-motile, non-spore-forming, and rod-shaped bacterial strains, HA2171T and HA2172T, were isolated from the feces of Chinese healthy don...

  • Article
  • Open Access
1 Citations
1,992 Views
19 Pages

A Genomic Surveillance Circuit for Emerging Viral Pathogens

  • Carlos S. Casimiro-Soriguer,
  • Maria Lara,
  • Andrea Aguado,
  • Carlos Loucera,
  • Francisco M. Ortuño,
  • Nicola Lorusso,
  • Jose M. Navarro-Marí,
  • Sara Sanbonmatsu-Gámez,
  • Pedro Camacho-Martinez and
  • Javier Perez-Florido
  • + 7 authors

Genomic surveillance has been crucial in monitoring the evolution and spread of SARS-CoV-2. In Andalusia (Spain), a coordinated genomic surveillance circuit was established to systematically sequence and analyze viral genomes across the region. This...

  • Article
  • Open Access
5 Citations
4,135 Views
12 Pages

Foodborne illnesses, particularly those caused by Salmonella enterica subsp. enterica Serovar Typhimurium, present a significant challenge to public health, especially within the seafood industry due to biofilm formation on foods. This study inv...

  • Review
  • Open Access
22 Citations
10,553 Views
29 Pages

Antibiotic-resistant Pseudomonas aeruginosa is a pathogen notorious for its resilience in clinical settings due to biofilm formation, efflux pumps, and the rapid acquisition of resistance genes. With traditional antibiotic therapy rendered ineffectiv...

  • Brief Report
  • Open Access
2 Citations
1,409 Views
10 Pages

Genomic Insights into Phosphorus Solubilization of Pseudomonas extremaustralis

  • Carolyn Mayer,
  • Catherine Urrutia,
  • Carol Jerez-Quezada,
  • Patricio Javier Barra and
  • Michel Abanto

Pseudomonas extremaustralis was first isolated from Antarctica and gained interest for its ability to thrive in extreme environmental conditions and degrade recalcitrant compounds. Some strains have been identified as phosphobacteria, which play a si...

  • Article
  • Open Access
5 Citations
1,403 Views
26 Pages

This study assessed the potential for natural anti-microbials to control Listeria monocytogenes in vacuum packed beef burgers. Minimum inhibitory and bactericidal concentration (MIC and MBC) results for natural anti-microbials (carvacrol; essential o...

  • Article
  • Open Access
7 Citations
2,717 Views
17 Pages

Cultivable and Non-Cultivable Approach to Bacteria from Undisturbed Soil with Plant Growth-Promoting Capacity

  • Lorena Jacqueline Gómez-Godínez,
  • Pedro Cisneros-Saguilán,
  • Dulce Darina Toscano-Santiago,
  • Yair Eduardo Santiago-López,
  • Saúl Neftalí Fonseca-Pérez,
  • Magali Ruiz-Rivas,
  • José Luis Aguirre-Noyola and
  • Gabriel García

Undisturbed soils are essential ecosystems with high microbial diversity. Microorganisms present in the soil can regulate biogeochemical cycles, making available and transforming different minerals in the soil, such as nitrogen, phosphorus and sulfur...

  • Article
  • Open Access
1 Citations
3,995 Views
23 Pages

Comprehensive Genomic Analysis of Klebsiella pneumoniae and Its Temperate N-15-like Phage: From Isolation to Functional Annotation

  • Reham Yahya,
  • Aljawharah Albaqami,
  • Amal Alzahrani,
  • Suha M. Althubaiti,
  • Moayad Alhariri,
  • Eisa T. Alrashidi,
  • Nada Alhazmi,
  • Mohammed A. Al-Matary and
  • Najwa Alharbi

Antibiotic resistance to Klebsiella pneumoniae poses a major public health threat, particularly in intensive care unit (ICU) settings. The emergence of extensively drug-resistant (XDR) strains complicates treatment options, requiring a deeper underst...

  • Article
  • Open Access
2 Citations
1,553 Views
14 Pages

Genomic Characterization of Enterococcus casseliflavus Isolated from Beef Cows and Calves

  • Sani-e-Zehra Zaidi,
  • Rahat Zaheer,
  • Athanasios Zovoilis,
  • Jayce Fossen,
  • Gary Van Domselaar,
  • Cheryl Waldner and
  • Tim A. McAllister

Enterococcus species are used as One Health indicators of antimicrobial resistance (AMR) in humans, animals, and the environment. A surveillance study in beef cows and calves isolated Enterococcus casseliflavus along with E. faecium, E. faecalis, and...

  • Article
  • Open Access
1,000 Views
15 Pages

Watermelon is one of the most important fruits in China, accounting for more than 70% of the world’s total output. Fusarium wilt of watermelon is the most common and serious disease in the cultivation of watermelon. It is mainly caused by Fusar...

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Microorganisms - ISSN 2076-2607