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Micromachines, Volume 8, Issue 8 (August 2017)
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Cover Story (view full-size image) Digital PCR, by counting molecular genetic scripts in partitioned reactions, is known for its [...] Read more. Digital PCR, by counting molecular genetic scripts in partitioned reactions, is known for its superior accuracy in nucleic acids analyses. Absolute quantification of the specific target DNA or RNA molecules has never been so well accepted and appreciated. Microfluidics has provided much needed solutions to digital PCR and helped in overcoming many technical challenges. Yet with its wide-reaching application, new challenges have emerged. Robustness and accessibility are to become the hot topics in digital PCR’s endless trial to ‘divide and conquer’. View Paper here