Search Results (386)

Metal pollution from metallurgical emissions poses serious environmental and public health risks in Kazakhstan. A replicated pot-culture experiment (n = 4) in a completely randomized design under controlled phytotron conditions evaluated biomass production and metal accumulation in six crop and forage species, alfalfa (Medicago sativa), amaranth (Amaranthus spp.), corn (Zea mays), mustard (Brassica juncea), rapeseed (Brassica napus), and sunflower (Helianthus annuus); three ornamental species, purple coneflower (Echinacea purpurea), marigold (Tagetes spp., ‘Tiger Eyes’), and sweet alyssum (Lobularia maritima); and three native wild plants, greater burdock (Arctium lappa), horse sorrel (Rumex confertus), and mug wort (Artemisia vulgaris). Plants were grown in soils collected from the Qarmet industrial zone in Temirtau, central Kazakhstan. Initial soil analysis revealed substantial mixed-metal contamination, ranked as Mn > Ba > Zn > Sr > Cr > Pb > Cu > Ni > B > Co. Mn reached 1059 mg·kg⁻¹, ~50-fold higher than B (22.7 mg·kg⁻¹). Ba (620 mg·kg⁻¹) exceeded FAO/WHO limits sixfold, Zn (204 mg·kg⁻¹) surpassed the lower threshold, and Pb (41.6 mg·kg⁻¹) approached permissible levels, while Cr, Cu, Ni, Co, and Sr were lower. Biomass production varied markedly among species: corn and sunflower produced the highest shoot biomass (126.8 and 60.9 g·plant⁻¹), whereas horse sorrel had the greatest root biomass (54.4 g·plant⁻¹). Root-to-shoot ratios indicated shoot-oriented growth (>1–8) in most species, except horse sorrel and burdock (<1). Metal accumulation was strongly species-specific. Corn and marigold accumulated Co, Pb, Cr, Mn, Ni, Cu, B, and Ba but showed limited translocation (transfer function, TF < 0.5), whereas sunflower, amaranth, and mug wort exhibited moderate to high translocation (TF > 0.8 to <1) for selected metals. Corn is recommended for high-biomass metal removal, marigold for stabilization, sunflower, horse sorrel, and mug wort for multi-metal extraction, and amaranth and coneflower for targeted Co, Ni, and Cu translocation, supporting sustainable remediation of industrially contaminated soils. Full article

The aims of this study were to evaluate the impact of probiotics (added as a starter sourdough and microcapsules) on gluten-free (GF) rice–amaranth steamed bread (SB) regarding physicochemical characteristics, sensory attributes, probiotic viability, and volatile organic compounds (VOCs). Also, probiotic viability, pH, total titratable acidity (TTA), moisture content, water activity, and texture were determined for 10 days of storage. GF-SB based on rice and amaranth was formulated and cooked at 90 ± 2 °C for 40 min. Three types of GF-SB were studied: control, with 30% sourdough fermented using Lactiplantibacillus plantarum NRRL B-4496 (GF-P), and with sourdough and encapsulated Limosilactobacillus reuteri DSM 17938 (GF-PC). The encapsulation yield was 94.9%. The viability of both probiotics was drastically reduced after steamed cooking, with losses ranging from 6 to 8 log₁₀ CFU/g. Sourdough decreased the pH (from 6.04 to 5.48–5.71) and hardness (control 46 N, sourdough ~25 N) while increasing lactic and acetic acids, moisture content (control 38%, sourdough ~46%), and water activity. Sourdough and probiotic capsules did not affect volume (~1.24 cm³/g), width-to-height ratio (~2.4), color, or sensory attributes. The VOCs revealed higher relative abundances of certain yeast-derived higher alcohols and oxidation-related carbonyl-trapping derivatives in control GF-SB, whereas bread with sourdough showed higher levels of long-chain hydrocarbons and esters, such as heptacosane and decanoic acid decyl ester. During the storage, Lpb. plantarum increased to ~3 log₁₀ CFU/g and Lim. reuteri remained steady. pH and TTA (0.03–0.04%) remained constant during storage. After 10 days of storage, hardness increased significantly (p < 0.05) in all GF-SB, doubling the initial values. Moisture content remained constant, while water activity decreased in GF-P (Δ = 0.025) and the control (Δ = 0.015). The use of sourdough in GF-SB improved texture, moisture content, and VOCs without modifying physical and sensory properties. Full article

Biomass ashes represent a promising secondary phosphorus (P) source, yet their agronomic performance depends on feedstock origin, processing, and crop-specific interactions. This study evaluated the P fertilizer efficacy of raw and processed biomass ashes derived from cereal straw and paludiculture biomass, compared with triple superphosphate (TSP), using two sequential greenhouse pot experiments with maize, amaranth, and blue lupine. Processed ash products, particularly compacted ashes and ash–straw mixtures, increased plant biomass and P uptake to levels comparable to or exceeding those achieved with TSP. The cumulative P uptake of the three crops reached up to 250–300 mg pot⁻¹ under processed ash treatments, exceeding the uptake under TSP (≈150–180 mg pot⁻¹) and the unfertilized control (≤80 mg pot⁻¹). However, crop-specific differences were observed: amaranth benefited most from the ash products, whereas combinations of ashes with lupine were less favorable. Beside acting as a P source, processed biomass ashes also increased soil pH by about 0.5 units, improved soil aggregation by increasing macroaggregates (>2 mm) to up to 20% compared with only about 7% in TSP and the control, and promoted favorable shifts in Hedley P fractions. Soil enzyme activities were governed primarily by crop species, with amaranth stimulating phosphatase activity the most. Further research should aim to refine crop-specific application strategies for processed biomass ashes and to elucidate their impacts on soil structure and P dynamics. Full article

Amaranth 11S globulin is a plant protein that is renowned for its high essential amino acid content and nutritional value. It has undergone modification through the insertion of antihypertensive peptides valine-tyrosine (VY), which act as angiotensin-converting enzyme (ACE) inhibitors. The expression of this protein was carried out in E. coli. Despite the potential of this protein, an efficient purification method is still required to allow its evaluation and subsequent application. This work proposes a procedure that allows for high purification and yield. After obtaining the purified proteins from the inclusion bodies and purifying them in an insoluble form, it was determined that this process did not affect their bioactivity. Full article

To address the soil degradation and growth inhibition caused by long-term monoculture of the medicinal plant Chrysanthemum morifolium Ramat. (Hangju), we conducted a controlled experiment comparing a monoculture (control) with seven different intercropping combinations. The intercropping treatments consisted of the main crop paired with pepper, schizonepeta, edible amaranth, dandelion, maize, soya, and purple perilla. Comprehensive assessments were conducted, encompassing plant growth parameters and rhizospheric soil properties. The soil properties included physicochemical characteristics, enzyme activities, and phenolic acid content (4-hydroxybenzoic acid, vanillic acid, and ferulic acid). The results indicated that intercropping significantly altered the rhizosphere environment of Hangju (p < 0.05). Purple perilla and maize emerged as particularly effective companion plants. Intercropping with purple perilla enhanced the aboveground biomass accumulation of Hangju and increased the activities of rhizosphere catalase, sucrase, β-glucosidase, and neutral phosphatase, although it also elevated the contents of three autotoxic phenolic acids. In contrast, intercropping with maize improved Hangju biomass and enhanced the activities of sucrase, urease, neutral phosphatase, and protease, while concurrently reducing the concentrations of all three phenolic acids. Overall, maize demonstrated optimal performance in comprehensively improving soil health by modulating enzyme activities, whereas purple perilla showed a distinct advantage in directly promoting plant growth. Full article

In recent decades, species within the genus Amaranthus L. (amaranth) have garnered growing global interest due to their exceptional nutritional value, functional properties, and agricultural versatility. Traditionally consumed as leafy vegetables or pseudo-cereals, several Amaranthus species are now receiving renewed attention in the context of the development of modern functional foods. This review evaluates the data on nutritional composition, health-promoting properties, and potential applications of Amaranthus spp. in sustainable food systems in peer-reviewed publications from the last 25 years. Amaranth is rich in high-quality proteins, essential amino acids, dietary fibre, vitamins, and minerals, positioning it as a significant factor in addressing malnutrition and enhancing food security. Furthermore, its bioactive compounds, such as flavonoids, phenolic acids, and peptides, exhibit antioxidant, anti-inflammatory, and hypocholesterolemic activities, suggesting its potential as a part of healthy diets, alleviating the risk of non-communicable diseases. The presence of anti-nutritional factors, including saponins, phytates, and oxalates, has also been explored, with implications for nutrient bioavailability and overall health effects. In addition to its nutritional advantages, Amaranthus spp. demonstrate strong adaptability to diverse climatic conditions, thus performing as a crop resilient under climate stress. Their olfactory and sensory attributes are also considered important for consumers’ acceptance and market integration. By synthesising traditional knowledge and contemporary scientific research, this review underscores the potential of Amaranthus spp. as a multifunctional food source that could support health promotion, climate resilience, and agricultural sustainability. Full article

The global demand for new ingredients and healthier food products is on the rise. Global challenges like rapid population growth, climate change, and emerging pandemics are putting a strain on food security for future generations. This makes it crucial to seek alternatives for producing nutrient-rich foods using more sustainable methods. In this context, proteins are an essential macronutrient for humanity. Plant-based proteins are becoming increasingly popular for the following reasons: their sustainability, as they have a lower environmental impact compared to animal-based proteins, provided they are consumed locally; their nutritional value, since they contain all the essential nutrients when consumed in a varied way and do not contain limiting amino acids; their potential accessibility; and the health benefits they offer. Consequently, the food industry is developing an increasing market of protein concentrates and isolates from plant sources using wet or dry methods. In particular, dry fractionation is expected to play a key role in enhancing food sustainability, as it allows protein enrichment without the use of water or energy-consuming operations. This review provides a detailed description of the application of dry fractionation method to Andean grains, with quinoa, amaranth, and kañiwa as prominent examples. The narrative review covers the essential primary processing and pretreatments, assesses the properties of the resulting fractions, and discusses their applications and future trends. This work aims to promote the development of innovative and sustainable food solutions. Full article

Weeds pose a ubiquitous challenge to researchers as a source of unintended variation on crop yield and other metrics in designed experiments, creating a need for practical and spatially comprehensive techniques for weed detection. To that end, imagery acquired using unmanned aerial systems (UASs) and classified using pixel-based, object-based, or neural network-based approaches provides researchers a promising avenue. However, in scenarios where spectral differences cannot be used to distinguish between crop and weed foliage, where physical overlap between crop and weed foliage obstructs object-based detection, or where large datasets are not available to train neural networks, alternative methods may be required. For instances where there is a consistent difference in height between crop and weed plants, a mask can be applied to a canopy height model (CHM) such that pixels are determined to be weed or non-weed based on height alone. The CHM Mask (CHMM) approach, which produces a measure of weed area coverage using UAS-acquired, red–green–blue imagery, was used to detect Palmer amaranth in Sweetpotato with an overall accuracy of 86% as well as explain significant variation in sweetpotato yield (p < 0.01). The CHMM approach contributes to the diverse methodologies needed to conduct weed detection in different agricultural settings. Full article

This study presents a methodological investigation of laser-induced breakdown spectroscopy (LIBS) for elemental analysis of quinoa and amaranth pseudocereals using a TEA CO₂ laser. Solid samples were prepared as pressed pellets, and reference data were obtained by ICP–OES. Synthetic solid standards were developed for calibration of selected elements (Ca, Fe, Zn, and Mg). Laser parameters were optimized based on the signal-to-noise ratio of characteristic spectral lines and applied to both pseudocereal samples. Emission lines of Mg, Ca, Fe, K, P, Zn, Al, Sr, and Cu were identified, and limits of detection were determined. Quantitative analysis used calibration curves from analyte-to-internal standard line intensity ratios, showing good linearity and agreement with reference values. Plasma diagnostics under optimized conditions revealed an average temperature of ~11,000 K and electron number densities of ~5 × 10¹⁶ cm⁻³ for both samples. Numerical plasma simulations confirmed the experimental results and provided additional insight into plasma composition and behavior. The developed LIBS methodology proved effective for multi-elemental analysis of pseudocereals and shows potential for application to other cereal and plant-based materials with similar composition. It should be noted that this methodology was demonstrated on pelletized samples prepared under controlled laboratory conditions; adaptation to rapid or field-based measurements would require alternative sample preparation strategies. This work provides a methodological framework and experimental validation for LIBS application in food compositional and nutritional analysis. Full article

Background: Cereal bars are convenient vehicles for incorporating ingredients with functional value. In this context, the study aimed to formulate bars enriched with quinoa, amaranth, and calafate (Berberis microphylla) and evaluate their instrumental texture, total phenolic compounds, antioxidant capacity, nutritional composition, and sensory evaluation. Methods: Four formulations were developed, a baseline cereal bar with balanced ingredients (F1), a pseudocereal-enriched bar (F2), a high-calafate bar (F3), and an oat-only control bar (F4). Texture was measured using uniaxial compression, total phenolic compounds (TPC) were determined by the Folin–Ciocalteu method, and antioxidant capacity was assessed by the DPPH assay. The nutritional composition was theoretically estimated using food composition tables and dietary reference intakes (DRIs). Sensory evaluation was performed using affective tests, including acceptability, preference, purchase intention, and sensory attributes. Results: The formulations differed significantly in instrumental hardness. F3 had the highest total phenol content and the highest antioxidant capacity. The estimated nutritional composition showed that the bars provide adequate energy and relevant micronutrients (Ca, Mg, Fe, Zn), as well as bioactive compounds from calafate. Sensory evaluation showed that F2 obtained the highest overall acceptance and the highest acceptability index. Purchase intention did not differ between formulations. In the evaluation of attributes, the results indicate that intermediate hardness maximizes acceptance, while softer (F1) or harder (F3–F4) bars are less preferred. Conclusions: The incorporation of calafate enhances the phenolic and antioxidant profile in F3, while an intermediate hardness linked to the greater use of expanded pseudocereals favors consumer acceptance in F2. The observed differences confirm that the formulation design enables the modulation of functional, mechanical, and sensory properties in cereal bars. Full article

Floating-tray hydroponics is expanding for microgreen production, yet evidence on substrate performance under natural-light greenhouses remains limited. This study compared perlite, coconut coir, and a 1:1 (v/v) perlite–coconut coir mixture for amaranth (Amaranthus cv. ‘Diablo Rojo’) grown at the end of summer in an unheated greenhouse (CRD; 4 replicates). Perlite significantly improved establishment and yield: emergence reached 72.6% versus 46.1% in coconut coir and 35.1% in the mixture, with fresh biomass of 297, 171, and 119 g m⁻², respectively. The proximate composition exhibited consistency across substrates (protein ≈ 32% DW; crude fiber ≈ 32% DW; crude fat ≈ 2.5% DW), whereas nitrate concentrations varied between 1300 and 2500 mg kg⁻¹ FW, irrespective of the substrate. Vitamin C and total phenolics showed no significant variation, and β-carotene exhibited only a slight, non-significant increase in coconut coir. In contrast, mineral composition was substrate-dependent: perlite increased Ca (3626 ppm DW) and Mg (1094 ppm DW), while P and Fe were unaffected; Na was higher in perlite. These results indicate that under natural-light, unheated greenhouses, bioactive compounds are mainly influenced by environmental variability, whereas root-zone aeration drives Ca/Mg enrichment and yield. Perlite-based floating-tray systems represent efficient, low-cost strategies with potentially lower environmental impact, suitable for scalable urban and smallholder microgreen production. These findings, derived from a single-season trial in one unheated greenhouse, should be regarded as preliminary yet consistent with previous studies on microgreens. Future multi-season and multi-species experiments will help to confirm and expand on these results. Full article

The objective of this study was to evaluate the structural, functional and morphological characterizations of ultrasonic-assisted extraction of crude prolamin from amaranth grain (Amaranthus hypochondriacus L.). A Box–Behnken design of response surface methodology (RSM) was employed to optimize the extraction parameters. The optimal extraction parameters included a solid/solvent ratio of 1:9 (g:mL), with 50% ethanol solution at 75 °C. Regarding the physicochemical characteristics, amaranth crude prolamin (ACP) possessed more orderly secondary structures (the proportions of α-helix and β-sheet were 43.65% and 25.52%, respectively), which is favorable for improving the object-holding capacity, such as higher oil- and water-holding capacity. The higher surface hydrophobicity of ACP was beneficial for self-assembly into microspheres at high ethanol concentrations. In general, ACP had a wider molecular weight distribution, higher zeta-potential and better emulsifying capacity (3.91 g VE/g prolamin). Thus, these results provide useful insight into the applications of amaranth prolamin. Full article

In this study, one of the main problems related to the development of new foods and the improvement of existing staple foods is examined. The effect of stalk pigweed flour in relation to the main raw material, wheat flour, at levels of 0%, 5%, 10%, and 15% was evaluated with respect to key characteristics of flour mixtures, dough, and biscuits with this additive. A selection of informative features was made, revealing that out of the 39 studied parameters, covering sensory, physicochemical, geometric, colorimetric, and spectral characteristics, only 19 proved to be informative. Principal component analysis showed that the relationship between amaranth green powder (AGP) concentration and the first two principal components explained up to 99% of the variance. The optimal addition level of 7.17% AGP was identified based on the convergence of biscuit characteristics. pH decreased from 6.55 to 6.28, electrical conductivity increased from 1075 to 3759 µS/cm, and sensory scores for aroma and taste peaked near 7% before declining at higher concentrations. It was demonstrated that the relationship between the amount of amaranth in biscuits and the first two principal components can be described with up to 99% accuracy. It was determined that the optimal amount of amaranth flour in biscuits is +7.17%. The results obtained provide a basis for further research into the rapid automated analysis of biscuits with added pigweed flour, which will contribute to the development of new foods with improved characteristics. It is suggested to carry out more research to study the effect of flours from other amaranth types, enhancing different varieties and cultivated in diverse ecological regions. This work also explores the viability of pigweed as a nutritious and sustainable flour alternative while providing a multivariate approach in view of newly developed bakery formulations. Full article

Effective implementation and optimization of organic amendments and other management practices is essential for sustainable organic vegetable production, yet needed information is lacking on the effects and benefits of different organic matter amendments and pest management approaches under Northeastern USA production conditions. The impacts of soil amendments of biochar or humus (soluble humate complex) in conjunction with compost, as well as the presence or absence of an early-season insect netting row cover (mesotunnels), were evaluated on soil chemical and biological properties, crop development and yield, and disease and pest issues in organic vegetable production, as represented by legume (green snap bean), cucurbit (green zucchini squash), and amaranth (garden beet) vegetable crops, in a three-year field trial in Maine. Composted cow manure and a commercial organic fertilizer alone were included as controls. All plots were either covered or not covered with a permeable insect netting row cover from the time of planting until flowering. All compost-based amendments increased soil pH; organic matter; microbial activity; crop yields; and K, Mg, and Ca content relative to a fertilizer-only treatment. Biochar amendments further increased soil pH, CEC, and Ca content above those of compost alone and also resulted in the overall highest yields of bean and zucchini but were not significantly greater than with compost amendment alone. Humus amendments did not improve soil characteristics, with some indications of potential reductions in emergence and yield. Insect netting substantially improved yield of zucchini (by 59%) and somewhat improved bean yield (by 11%), in addition to improving plant emergence and reducing insect leaf damage, but it did not reduce powdery mildew on zucchini or provide any significant benefits for beets. These results help define specific management practices to improve organic vegetable production and provide useful information and options for growers. Full article

This research evaluated the influence of lactic acid bacteria and cellulase, individually or in combination, on the quality of mixed amaranth and sweet potato vine silages. The experiment included four groups: control group with no additives addition (CG), added cellulase group (AS1), added lactic acid bacteria group (AS2) and combined supplementation group (AS3), with five replicates per group. The ensiling period lasted for 60 days. Parameters of silage, including chemical components, fermentation profile, aerobic stability, and in vitro nutrient digestibility, were determined. The results revealed that the quality of amaranth and sweet potato vine mixed silage was improved to a certain degree after addition of two additives individually. Combining these additives observably increased (p < 0.05) the lactic acid and crude protein contents and decreased the pH, ratio of ammonia nitrogen to total nitrogen and neutral detergent fiber content of mixed silage. Compared with the CG and AS1 groups, the number of lactic acid bacteria in the AS3 group increased significantly (p < 0.05), while aerobic bacteria and mold counts showed the opposite tendency. Also, the in vitro dry matter, crude protein and neutral detergent fiber digestibility of the AS3 group were higher (p < 0.05) than those of the CG group. Combined inoculation observably reduced (p < 0.05) the ammonia nitrogen concentration and increased (p < 0.05) the propionic and butyric acid concentrations of mixed silage under in vitro incubation. In summary, the inoculation of lactic acid bacteria and cellulase can enhance the fermentation profile and nutritional values of mixed silage made from amaranth and sweet potato vine, and the best improvement effects are obtained by the combined utilization of the two additives. Full article