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4 Results Found

  • Review
  • Open Access
42 Citations
11,585 Views
28 Pages

High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives

  • Magdalena Wójcik,
  • Aline Telzerow,
  • Wim J. Quax and
  • Ykelien L. Boersma

20 October 2015

Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have great...

  • Feature Paper
  • Article
  • Open Access
35 Citations
7,172 Views
12 Pages

Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers

  • Phalguni Tewari Kumar,
  • Deborah Decrop,
  • Saba Safdar,
  • Ioannis Passaris,
  • Tadej Kokalj,
  • Robert Puers,
  • Abram Aertsen,
  • Dragana Spasic and
  • Jeroen Lammertyn

15 March 2020

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled metho...

  • Article
  • Open Access
12 Citations
5,338 Views
13 Pages

Portable Digital Monitoring System for Sarcopenia Screening and Diagnosis

  • Eduardo Teixeira,
  • Lucimére Bohn,
  • José Pedro Guimarães and
  • Inês Marques-Aleixo

25 October 2022

Sarcopenia is a well-known highly prevalent muscle disease that severely impairs overall physical performance in elders, inducing a massive health-related economic burden. The widespread screening, diagnosis and treatment of sarcopenia are pivotal to...

  • Article
  • Open Access
38 Citations
9,770 Views
14 Pages

Efficient Delivery of DNA Using Lipid Nanoparticles

  • Lishan Cui,
  • Serena Renzi,
  • Erica Quagliarini,
  • Luca Digiacomo,
  • Heinz Amenitsch,
  • Laura Masuelli,
  • Roberto Bei,
  • Gianmarco Ferri,
  • Francesco Cardarelli and
  • Giulio Caracciolo
  • + 4 authors

DNA vaccination has been extensively studied as a promising strategy for tumor treatment. Despite the efforts, the therapeutic efficacy of DNA vaccines has been limited by their intrinsic poor cellular internalization. Electroporation, which is based...