Toxics

16 pages, 1606 KB

Open AccessEditor’s ChoiceArticle

Biomonitoring PhIP, a Potential Prostatic Carcinogen, in the Hair of Healthy Men of African and European Ancestry

by Robert J. Turesky, Clarence Jones, Jingshu Guo, Kari Cammerrer, Laura A. Maertens, Emmanuel S. Antonarakis, Zhanni Lu and Logan G. Spector

Toxics 2025, 13(1), 42; https://doi.org/10.3390/toxics13010042 - 8 Jan 2025

Viewed by 1736

Abstract

Heterocyclic aromatic amines (HAAs), formed during the cooking of meat, are potential human carcinogens, underscoring the need for long-lived biomarkers to assess exposure and cancer risk. Frequent consumption of well-done meats containing 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a prevalent HAA that is a prostatic [...] Read more.

Heterocyclic aromatic amines (HAAs), formed during the cooking of meat, are potential human carcinogens, underscoring the need for long-lived biomarkers to assess exposure and cancer risk. Frequent consumption of well-done meats containing 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a prevalent HAA that is a prostatic carcinogen in rodents and DNA-damaging agent in human prostate cells, has been linked to aggressive prostate cancer (PC) pathology. African American (AA) men face nearly twice the risk for developing and dying from PC compared to White men. We previously demonstrated that scalp hair is a reliable biospecimen for measuring PhIP intake using liquid chromatography-mass spectrometry. This study aimed to determine whether PhIP dietary intake is higher in AA men, potentially contributing to this health disparity. Healthy AA men were found to have a significantly higher mean hair PhIP level (2.12-fold) than White men on free-choice diets. However, this difference was not statistically significant after adjusting for melanin content. Further research is needed to understand how hair pigmentation, follicular density, and other morphological features of hair influence PhIP accumulation. These insights can improve the accuracy of using hair PhIP levels as a biomarker for exposure and its potential associations with cancer risk. Full article

(This article belongs to the Section Novel Methods in Toxicology Research)

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17 pages, 916 KB

Open AccessEditor’s ChoiceArticle

Optimization of Toxicity, Biodegradability, and Skin Irritation in Formulations Containing Mixtures of Anionic and Nonionic Surfactants Combined with Silica Nanoparticles

by Manuela Lechuga, Mercedes Fernández-Serrano, Josefa Núñez-Olea, Juan Francisco Martínez-Gallegos and Francisco Ríos

Toxics 2025, 13(1), 43; https://doi.org/10.3390/toxics13010043 - 8 Jan 2025

Cited by 4 | Viewed by 3615

Abstract

Surfactants play a crucial role in various industrial applications, including detergents and personal care products. However, their widespread use raises concerns due to their potential environmental impact and health risks, particularly in aquatic ecosystems, where they can disrupt the balance of marine life [...] Read more.

Surfactants play a crucial role in various industrial applications, including detergents and personal care products. However, their widespread use raises concerns due to their potential environmental impact and health risks, particularly in aquatic ecosystems, where they can disrupt the balance of marine life and accumulate in water sources, posing challenges to sustainable development. This study investigates the environmental and health implications of anionic and nonionic surfactants, focusing on their toxicity, biodegradation, and skin irritation potential profiles, especially when combined with silica nanoparticles. Toxicity assessments were conducted using bacteria Vibrio fischeri for aquatic toxicity and Lepidium sativum seeds for terrestrial plant effects, revealing that individual surfactants like the anionic alkyl ether carboxylic acid EC-R_12–14E₃ exhibit high toxicity levels, while the nonionic fatty-alcohol ethoxylate FAE-R_12–14E₁₁ shows comparatively lower environmental impact. The toxicity of surfactant mixtures was analysed, revealing both antagonistic and synergistic effects depending on the surfactants used. The addition of silica nanoparticles generally mitigates the overall toxicity of surfactants, whether used individually or in mixtures. Biodegradation studies followed OECD 301E and 301F guidelines, indicating that individual surfactants generally meet or approach the mineralization threshold, whereas the addition of nanoparticles reduced biodegradation efficacy. Potential skin irritation was predicted through the zein number (ZN), finding that some surfactant combinations with silica nanoparticles reduce irritation levels, highlighting their potential for safer formulation in products that come into direct contact with the skin. Overall, the findings emphasize the need for careful selection of surfactant mixtures and nanoparticle integration to minimize environmental toxicity and potential skin irritation and increase their biodegradability. Full article

(This article belongs to the Special Issue Acute Toxicity, Environmental Impact Assessment and Human Safety of Surfactants)

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17 pages, 3171 KB

Open AccessEditor’s ChoiceArticle

Activation of ClO₂ by Nanoscale Zero-Valent Iron for Efficient Soil Polycyclic Aromatic Hydrocarbon Degradation: New Insight into the Relative Contribution of Fe(IV) and Hydroxyl Radicals

by Xiaojun Hu, Xiaorong Xing, Fan Zhang, Bingzhi Li, Senlin Chen, Bo Wang, Jiaolong Qin and Jie Miao

Toxics 2025, 13(1), 36; https://doi.org/10.3390/toxics13010036 - 5 Jan 2025

Cited by 1 | Viewed by 1130

Abstract

Recently, the activation of chlorine dioxide (ClO₂) by metal(oxide) for soil remediation has gained notable attention. However, the related activation mechanisms are still not clear. Herein, the variation of iron species and ClO₂, the generated reactive oxygen species, and [...] Read more.

Recently, the activation of chlorine dioxide (ClO₂) by metal(oxide) for soil remediation has gained notable attention. However, the related activation mechanisms are still not clear. Herein, the variation of iron species and ClO₂, the generated reactive oxygen species, and the toxicity of the degradation intermediates were explored and evaluated with nanoscale zero-valent iron (nFe⁰) being employed to activate ClO₂ for soil polycyclic aromatic hydrocarbon (PAH) removal. With an optimized ClO₂/nFe⁰ molar ratio of 15:1 and a soil/water ratio of 3:1, the degradation efficiency of phenanthrene improved 12% in comparison with that of a ClO₂-alone system. The presence of nFe⁰ significantly promoted ClO₂ consumption (improved 85.4%) but restrained ClO₂⁻ generation (reduced 22.5%). The surface Fe(II) and soluble Fe(II) in the ClO₂/nFe⁰ system was 2.0-fold and 2.8-fold that in the nFe⁰ system after 2 min. Electron paramagnetic resonance analysis, along with quenching experiments, revealed that Fe(IV), HOCl, and •OH dominated phenanthrene degradation in a ClO₂/nFe⁰ system, with oxidation contributions, respectively, of 34.3%, 52.8% and 12.9%. The degradation intermediates of PAHs in the ClO₂/nFe⁰ system had lower estimated toxicity than those of the ClO₂ system. The lettuces grown in ClO₂/nFe⁰-treated soil displayed better results in bioassay indexes than those grown in ClO₂-treated soil. This study offers new perspectives for the remediation of organic-pollutant-contaminated soil by using metal-activated ClO₂ technology. Full article

(This article belongs to the Special Issue Novel Remediation Strategies for Soil Pollution)

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26 pages, 557 KB

Open AccessEditor’s ChoiceSystematic Review

A Systematic Review of Parental Occupational Pesticide Exposure and Geographical Proximity to Agricultural Fields in Association with Neural Tube Defects

by Karoline Felisbino, Shayane da Silva Milhorini, Nathalia Kirsten, Mariane Yoshie Sato, Davi Paula da Silva, Juliana Danna Kulik and Izonete Cristina Guiloski

Toxics 2025, 13(1), 34; https://doi.org/10.3390/toxics13010034 - 4 Jan 2025

Cited by 3 | Viewed by 2756

Abstract

Neural tube defects (NTDs) are the second most common congenital anomaly and have been widely associated with exposure to pesticides. This systematic review which analyzes the association between parental exposure to pesticides and NTDs was conducted in accordance with Preferred Reporting Items for [...] Read more.

Neural tube defects (NTDs) are the second most common congenital anomaly and have been widely associated with exposure to pesticides. This systematic review which analyzes the association between parental exposure to pesticides and NTDs was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The search strategy was designed considering the population, exposure, controls, outcomes, and study design (PECOS). The inclusion criteria were epidemiological studies involving pesticides exposure during embryonic development, written in English, Portuguese, or Spanish, and performed in 12 databases. Based on the eligibility criteria, 16 articles were selected for analysis. The most frequently assessed NTDs were anencephaly and spina bifida, with 14 studies investigating each of these conditions. The assessment of pesticide exposure was based on parental occupation and residential proximity to agricultural fields. Studies differed regarding the pesticides assessed, exposure windows, and parents exposed. Regarding the outcomes, it was observed that geographic proximity to agricultural fields and a greater association with family members exposed to pesticides were found in mothers and neonates with NTDs. In relation to occupational exposure, some articles found an association with NTDs, while others did not. Therefore, an analysis of the available evidence suggests that pesticides are a risk factor in the development of NTDs. Full article

(This article belongs to the Special Issue Pesticides and Human Health: Between Toxicology and Epidemiology)

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23 pages, 955 KB

Open AccessEditor’s ChoiceArticle

A Proof-of-Concept for Safety Evaluation of Inhalation Exposure to Known Respiratory Irritants Using In Vitro and In Silico Methods

by Nikaeta Sadekar, Holger Peter Behrsing, Tanja Hansen, Vivek Patel, Hazel Paulo, Alex Rae, Detlef Ritter, Katharina Schwarz and Anne Marie Api

Toxics 2025, 13(1), 35; https://doi.org/10.3390/toxics13010035 - 4 Jan 2025

Cited by 3 | Viewed by 2690

Abstract

There is increased interest in developing non-animal test systems for inhalation exposure safety assessments. However, defined methodologies are absent for predicting local respiratory effects from inhalation exposure to irritants. The current study introduces a concept for applying in vitro and in silico methods [...] Read more.

There is increased interest in developing non-animal test systems for inhalation exposure safety assessments. However, defined methodologies are absent for predicting local respiratory effects from inhalation exposure to irritants. The current study introduces a concept for applying in vitro and in silico methods for inhalation exposure safety assessment. Three in vitro systems, representing the upper (MucilAir™—nasal epithelial tissue) and lower (A549 cells and human precision-cut lung slices) human respiratory regions, were exposed to six respiratory irritants. These irritant exposures were conducted as liquid droplets, aerosol, or vapors, and samples were collected over 24 h. Cytotoxicity, cytokine release, epithelial resistance, oxidative stress, and mitochondrial membrane potential were measured. To determine the human relevance of in vitro exposures, airway surface depositions were predicted by simulating airborne concentrations equivalent to the Cramer class III inhalation threshold of toxicological concern limit of 0.47 mg/person/day using an in silico model. A > 100-fold margin of exposure was calculated comparing lowest concentrations showing in vitro effects to in silico simulated values. While further studies are needed, this manuscript presents a basic requirement for employing non-animal methods to inform inhalation exposure safety assessments by combining in vitro and in silico assays. Full article

(This article belongs to the Special Issue Feature Papers in the Novel Methods in Toxicology Research)

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15 pages, 3730 KB

Open AccessEditor’s ChoiceArticle

A Study on Dust Storm Pollution and Source Identification in Northwestern China

by Hongfei Meng, Feiteng Wang, Guangzu Bai and Huilin Li

Toxics 2025, 13(1), 33; https://doi.org/10.3390/toxics13010033 - 3 Jan 2025

Cited by 3 | Viewed by 2098

Abstract

In April 2023, a major dust storm event in Lanzhou attracted widespread attention. This study provides a comprehensive analysis of the causes, progression, and dust sources of this event using multiple data sources and methods. Backward trajectory analysis using the HYSPLIT model was [...] Read more.

In April 2023, a major dust storm event in Lanzhou attracted widespread attention. This study provides a comprehensive analysis of the causes, progression, and dust sources of this event using multiple data sources and methods. Backward trajectory analysis using the HYSPLIT model was employed to trace the origins of the dust, while FY-2H satellite data provided high-resolution dust distribution patterns. Additionally, the MAIAC AOD product was used to analyze Aerosol Optical Depth, and concentration-weighted trajectory (CWT) analysis was used to identify key dust source regions. The study found that PM₁₀ played a dominant role in the storm, and the AOD values during the storm in Lanzhou were significantly higher than the annual average, highlighting the severe impact on regional air quality. Key meteorological conditions influencing the storm’s occurrence were analyzed, including the formation and eastward movement of a high-potential ridge, convection driven by diurnal temperature variations, and surface temperature increases coupled with decreased relative humidity, which together promoted the generation and development of dust. Backward trajectory and dust distribution analyses revealed that the dust primarily originated from Central Asia, western Mongolia, Xinjiang, and Gansu. From the 19th to the 21st, the dust distribution showed similarities between day and night, with a noticeable increase in dust concentration from night to day due to strong vertical atmospheric mixing. To mitigate the impacts of future dust storms, this study highlights both short-term and long-term strategies, including enhanced monitoring systems, public health advisories, and vegetation restoration in key source regions. Strengthening regional and international cooperation for transboundary dust management is also emphasized as critical for sustainable mitigation efforts. These findings are significant for understanding and predicting the causes, characteristics, and environmental impacts of dust storms in Lanzhou and the Northwestern region. Full article

(This article belongs to the Special Issue Atmospheric Aerosols: Source Apportionment, Characterizations, and Detection, 2nd Edition)

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15 pages, 7972 KB

Open AccessEditor’s ChoiceArticle

PM_2.5 Exposure Induces Glomerular Hyperfiltration in Mice in a Gender-Dependent Manner

by Hao Wang, Li Ma, Yuqiong Guo, Lingyu Ren, Guangke Li and Nan Sang

Toxics 2024, 12(12), 878; https://doi.org/10.3390/toxics12120878 - 1 Dec 2024

Cited by 1 | Viewed by 9537

Abstract

As one of the most common air pollutants, fine particulate matter (PM_2.5) increases the risk of diseases in various systems, including the urinary system. In the present study, we exposed male and female C57BL/6J mice to PM_2.5 for 8 weeks. [...] Read more.

As one of the most common air pollutants, fine particulate matter (PM_2.5) increases the risk of diseases in various systems, including the urinary system. In the present study, we exposed male and female C57BL/6J mice to PM_2.5 for 8 weeks. Examination of renal function indices, including creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and urinary microalbumin, indicated that the kidneys of female mice, not male mice, underwent early renal injury, exhibiting glomerular hyperfiltration. Meanwhile, pathological staining showed that the kidneys of female mice exhibited enlarged glomerulus that filled the entire Bowman’s capsule in the female mice. Afterward, we explored the potential causes and mechanisms of glomerular hyperfiltration. Variations in mRNA levels of key genes involved in the renin–angiotensin system (RAS) and kallikrein–kinin system (KKS) demonstrated that PM_2.5 led to elevated glomerular capillary hydrostatic pressure in female mice by disturbing the balance between the RAS and KKS, which in turn increased the glomerular filtration rate (GFR). In addition, we found that PM_2.5 increased blood glucose levels in the females, which enhanced tubular reabsorption of glucose, attenuated macular dense sensory signaling, induced renal hypoxia, and affected adenosine triphosphate (ATP) synthesis, thus attenuating tubuloglomerular feedback (TGF)-induced afferent arteriolar constriction and leading to glomerular hyperfiltration. In conclusion, this study indicated that PM_2.5 induced glomerular hyperfiltration in female mice by affecting RAS/KKS imbalances, as well as the regulation of TGF; innovatively unveiled the association between PM_2.5 subchronic exposure and early kidney injury and its gender dependence; enriched the toxicological evidence of PM_2.5 and confirmed the importance of reducing ambient PM_2.5 concentrations. Full article

(This article belongs to the Special Issue Toxicity and Human Health Assessment of Air Pollutants)

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34 pages, 4477 KB

Open AccessEditor’s ChoiceReview

The Mechanisms of Cadmium Toxicity in Living Organisms

by Slavena Davidova, Viktor Milushev and Galina Satchanska

Toxics 2024, 12(12), 875; https://doi.org/10.3390/toxics12120875 - 30 Nov 2024

Cited by 19 | Viewed by 8006

Abstract

Cadmium (Cd) is a toxic metal primarily found as a by-product of zinc production. Cd was a proven carcinogen, and exposure to this metal has been linked to various adverse health effects, which were first reported in the mid-19th century and thoroughly investigated [...] Read more.

Cadmium (Cd) is a toxic metal primarily found as a by-product of zinc production. Cd was a proven carcinogen, and exposure to this metal has been linked to various adverse health effects, which were first reported in the mid-19th century and thoroughly investigated by the 20th century. The toxicokinetics and dynamics of Cd reveal its propensity for long biological retention and predominant storage in soft tissues. Until the 1950s, Cd pollution was caused by industrial activities, whereas nowadays, the main source is phosphate fertilizers, which strongly contaminate soil and water and affect human health and ecosystems. Cd enters the human body mainly through ingestion and inhalation, with food and tobacco smoke being the primary sources. It accumulates in various organs, particularly the kidney and liver, and is known to cause severe health problems, including renal dysfunction, bone diseases, cardiovascular problems, and many others. On a cellular level, Cd disrupts numerous biological processes, inducing oxidative stress generation and DNA damage. This comprehensive review explores Cd pollution, accumulation, distribution, and biological impacts on bacteria, fungi, edible mushrooms, plants, animals, and humans on a molecular level. Molecular aspects of carcinogenesis, apoptosis, autophagy, specific gene expression, stress protein synthesis, and ROS formation caused by Cd were discussed as well. This paper also summarizes how Cd is removed from contaminated environments and the human body. Full article

(This article belongs to the Special Issue Cadmium and Trace Elements Toxicity)

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22 pages, 1648 KB

Open AccessEditor’s ChoiceReview

Presence of Potentially Toxic Elements in Historical Mining Areas in the North-Center of Mexico and Possible Bioremediation Strategies

by Victor Manuel Escot-Espinoza, Susana Rodríguez-Márquez, Jorge Briseño-Bugarín, Maria Argelia López-Luna and Juan Armando Flores de la Torre

Toxics 2024, 12(11), 813; https://doi.org/10.3390/toxics12110813 - 13 Nov 2024

Cited by 3 | Viewed by 3333

Abstract

This paper provides an overview of the impacts of mining-related environmental liabilities on humans, soils, sediments, surface water and groundwater across various mining districts in Zacatecas, Mexico. An analysis has been carried out on the areas of the state most affected by the [...] Read more.

This paper provides an overview of the impacts of mining-related environmental liabilities on humans, soils, sediments, surface water and groundwater across various mining districts in Zacatecas, Mexico. An analysis has been carried out on the areas of the state most affected by the presence of potentially toxic elements (PTEs) such as arsenic, lead, cadmium, copper, chromium and zinc, identifying priority areas for environmental assessment and remediation. Likewise, a review of the concentrations of PTEs reported in different environmental matrices of the state’s mining areas with the presence of environmental liabilities was carried out, most of which exceed the maximum permissible limits established by Mexican and international regulations, generating an environmental risk for the populations near these districts due to their potential incorporation into the food chain. Additionally, this study explores research focused on the biostabilization of PTEs using microorganisms with specific metabolic activities. Phytoremediation is presented as a viable tool for the stabilization and elimination of PTEs, in which endemic plants from arid–semi-arid climates have shown favorable results in terms of the phytostabilization and phytoextraction processes of the PTEs present in mining waste. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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21 pages, 4624 KB

Open AccessEditor’s ChoiceArticle

A Novel Machine Learning Model and a Web Portal for Predicting the Human Skin Sensitization Effects of Chemical Agents

by Ricardo Scheufen Tieghi, José Teófilo Moreira-Filho, Holli-Joi Martin, James Wellnitz, Miguel Canamary Otoch, Marielle Rath, Alexander Tropsha, Eugene N. Muratov and Nicole Kleinstreuer

Toxics 2024, 12(11), 803; https://doi.org/10.3390/toxics12110803 - 7 Nov 2024

Cited by 3 | Viewed by 3790

Abstract

Skin sensitization is a significant concern for chemical safety assessments. Traditional animal assays often fail to predict human responses accurately, and ethical constraints limit the collection of human data, necessitating a need for reliable in silico models of skin sensitization prediction. This study [...] Read more.

Skin sensitization is a significant concern for chemical safety assessments. Traditional animal assays often fail to predict human responses accurately, and ethical constraints limit the collection of human data, necessitating a need for reliable in silico models of skin sensitization prediction. This study introduces HuSSPred, an in silico tool based on the Human Predictive Patch Test (HPPT). HuSSPred aims to enhance the reliability of predicting human skin sensitization effects for chemical agents to support their regulatory assessment. We have curated an extensive HPPT database and performed chemical space analysis and grouping. Binary and multiclass QSAR models were developed with Bayesian hyperparameter optimization. Model performance was evaluated via five-fold cross-validation. We performed model validation with reference data from the Defined Approaches for Skin Sensitization (DASS) app. HuSSPred models demonstrated strong predictive performance with CCR ranging from 55 to 88%, sensitivity between 48 and 89%, and specificity between 37 and 92%. The positive predictive value (PPV) ranged from 84 to 97%, versus negative predictive value (NPV) from 22 to 65%, and coverage was between 75 and 93%. Our models exhibited comparable or improved performance compared to existing tools, and the external validation showed the high accuracy and sensitivity of the developed models. HuSSPred provides a reliable, open-access, and ethical alternative to traditional testing for skin sensitization. Its high accuracy and reasonable coverage make it a valuable resource for regulatory assessments, aligning with the 3Rs principles. The publicly accessible HuSSPred web tool offers a user-friendly interface for predicting skin sensitization based on chemical structure. Full article

(This article belongs to the Special Issue Skin Sensitization Testing Using New Approach Methodologies)

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16 pages, 11135 KB

Open AccessEditor’s ChoiceArticle

The Acute Toxicity and Cardiotoxic Effects of Protocatechuic Aldehyde on Juvenile Zebrafish

by Jiufeng Yin, Hui Wang, Feng Zhao, Dan Liang, Wenqing Yang and Dan Zhang

Toxics 2024, 12(11), 799; https://doi.org/10.3390/toxics12110799 - 3 Nov 2024

Cited by 2 | Viewed by 3347

Abstract

Protocatechuic aldehyde (PCA) is a natural phenolic acid compound with pharmacological effects such as anti-oxidative stress, antibacterial, anti-apoptotic, anti-inflammatory, anti-platelet aggregation, and anti-tumor. Despite the favorable therapeutic effects of PCA, it is imperative to recognize that adverse drug reactions can arise even with [...] Read more.

Protocatechuic aldehyde (PCA) is a natural phenolic acid compound with pharmacological effects such as anti-oxidative stress, antibacterial, anti-apoptotic, anti-inflammatory, anti-platelet aggregation, and anti-tumor. Despite the favorable therapeutic effects of PCA, it is imperative to recognize that adverse drug reactions can arise even with satisfactory quality assurance measures and during standard clinical application and dosing. Additionally, the acute toxicity and cardiotoxic sequelae of PCA are frequently under reported in the available documentation. To investigate the acute toxicity and cardiotoxic effects of PCA, the present study comprehensively assessed the acute toxicity and cardiotoxic effects of PCA by administering different concentrations of PCA and by monitoring the phenotypic changes in zebrafish, using AB wild-type Tg(cmlc2:EGFP) zebrafish as the experimental model organism. Meanwhile, the target genes of PCA that may cause cardiotoxicity were predicted and validated using a network pharmacology approach. Our findings indicated that PCA exhibited severe acute toxicity and cardiotoxic effects in zebrafish at 70 μg/mL and 80 μg/mL. Furthermore, PIK3CA, PARP1, and GSK3β may be involved in the mechanism of action of the cardiotoxicity-inducing effects of this compound. The present investigation has afforded a deeper insight into the acute toxicity and cardiotoxic impacts of PCA on zebrafish and has established a significant theoretical foundation for the evaluation of toxicity in pharmaceuticals incorporating PCA. Full article

(This article belongs to the Special Issue Drug Metabolism and Toxicological Mechanisms)

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17 pages, 273 KB

Open AccessEditor’s ChoiceReview

Research Progress on Membrane Separation Technology for Oily Wastewater Treatment

by Yichang Wang, Yu Zhang, Liang Liang, Feng Tu, Zhongjian Li, Xianjin Tang, Li Dai and Lingli Li

Toxics 2024, 12(11), 794; https://doi.org/10.3390/toxics12110794 - 31 Oct 2024

Cited by 16 | Viewed by 4213

Abstract

This paper presents the research progress and future prospects of membrane separation technology for treating oily wastewater. It discusses various treatment methods tailored to different sources and characteristics of oily wastewater, summarizing the features of different membrane separation technologies and the latest advancements [...] Read more.

This paper presents the research progress and future prospects of membrane separation technology for treating oily wastewater. It discusses various treatment methods tailored to different sources and characteristics of oily wastewater, summarizing the features of different membrane separation technologies and the latest advancements in their application. The paper concludes by emphasizing the need for future research to focus on developing environmentally friendly and efficient coupled membrane treatment technologies, optimizing membrane material design and enhancing the environmental benefits of oily wastewater treatment. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

23 pages, 839 KB

Open AccessEditor’s ChoiceReview

Micro(nano)plastic and Related Chemicals: Emerging Contaminants in Environment, Food and Health Impacts

by Juliana G. R. de Carvalho, Helga Coelho Augusto, Ricardo Ferraz, Cristina Delerue-Matos and Virgínia Cruz Fernandes

Toxics 2024, 12(10), 762; https://doi.org/10.3390/toxics12100762 - 20 Oct 2024

Cited by 4 | Viewed by 4924

Abstract

Microplastic pollution is a problem of increasing concern in food, and while food safety issues around the world are serious, an increasing number of food safety issues related to microplastics have become the focus of people’s attention. The presence of microplastics in food [...] Read more.

Microplastic pollution is a problem of increasing concern in food, and while food safety issues around the world are serious, an increasing number of food safety issues related to microplastics have become the focus of people’s attention. The presence of microplastics in food is a worldwide problem, and they are present in all kinds of foods, foods of both animal and plant origin, food additives, drinks, plastic food packaging, and agricultural practices. This can cause problems for both humans and the environment. Microplastics have already been detected in human blood, heart, placenta, and breastmilk, but their effects in humans are not well understood. Studies with mammals and human cells or organoids have given perspective about the potential impact of micro(nano)plastics on human health, which affect the lungs, kidneys, heart, neurological system, and DNA. Additionally, as plastics often contain additives or other substances, the potentially harmful effects of exposure to these substances must also be carefully studied before any conclusions can be drawn. The study of microplastics is very complex as there are many factors to account for, such as differences in particle sizes, constituents, shapes, additives, contaminants, concentrations, etc. This review summarizes the more recent research on the presence of microplastic and other plastic-related chemical pollutants in food and their potential impacts on human health. Full article

(This article belongs to the Special Issue Environmental Exposure to Toxic Chemicals and Human Health II)

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11 pages, 657 KB

Open AccessEditor’s ChoiceReview

The Role of Copper in Alzheimer’s Disease Etiopathogenesis: An Updated Systematic Review

by Angela Sabalic, Veronica Mei, Giuliana Solinas and Roberto Madeddu

Toxics 2024, 12(10), 755; https://doi.org/10.3390/toxics12100755 - 17 Oct 2024

Cited by 7 | Viewed by 3185

Abstract

Background: Alzheimer’s disease (AD) is the most common cause of dementia and cognitive decline in the elderly. Although the etiology of AD is unknow, an increase in amyloid precursor protein (APP) leads to the toxic aggregation of Aβ plaques. Several factors, such as [...] Read more.

Background: Alzheimer’s disease (AD) is the most common cause of dementia and cognitive decline in the elderly. Although the etiology of AD is unknow, an increase in amyloid precursor protein (APP) leads to the toxic aggregation of Aβ plaques. Several factors, such as hypertension, diabetes, dyslipidemia, smoking, hormonal changes, and metal exposure, could increase the risk of developing AD. In this review, we will examine the role of copper (Cu) in the pathophysiology of AD, as well as the mechanisms involved in neurotoxicity and cognitive decline. Methods: This review was conducted in accordance with PRISMA guidelines. We performed a comprehensive literature analysis over the last ten years on AD and Cu. Only late-onset Alzheimer’s disease was considered; only studies on elderly people of both sexes were included. Results: A total of seven articles were picked for this review, three studies focused on non-ceruloplasmin-bound Copper (non-Cp-Cu) and four on ceruloplasmin-bound Copper (Cp-Cu). The results showed higher Cu concentrations in patients compared to healthy controls. Conclusions: Elevated concentrations of Cu may contribute to the progression of AD, potentially interacting with ATP7B mutations, oxidative stress (OS), and amyloid-β plaques. Future research is needed to provide more robust evidence and better characterize the relationship between AD and Cu. Full article

(This article belongs to the Special Issue Heavy Metal Induced Neurotoxicity)

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25 pages, 1383 KB

Open AccessEditor’s ChoiceReview

Health Implications of Widespread Micro- and Nanoplastic Exposure: Environmental Prevalence, Mechanisms, and Biological Impact on Humans

by Olivia-Teodora Preda, Ana-Maria Vlasceanu, Cristina Veronica Andreescu, Aristidis Tsatsakis, Yaroslav Mezhuev, Carolina Negrei and Daniela Luiza Baconi

Toxics 2024, 12(10), 730; https://doi.org/10.3390/toxics12100730 - 10 Oct 2024

Cited by 12 | Viewed by 6447

Abstract

The increasing awareness of the potential health risks associated with microplastics’ (MPs) and nanoplastics’ (NPs) presence in the environment has led to a significant rise in research focused on these particles over the past few years. This review focuses on the research on [...] Read more.

The increasing awareness of the potential health risks associated with microplastics’ (MPs) and nanoplastics’ (NPs) presence in the environment has led to a significant rise in research focused on these particles over the past few years. This review focuses on the research on MPs’/NPs’ presence and spread, pathways of exposure, toxicological effects on human health and legal framework related to MP/NP challenges. Several research projects have aimed to assess their potential harm to human health, focusing on different systems and organs. After exposure (independent of the pathway), these hazards reach the blood stream and concentrate in different organs. Further, they are responsible for harmful changes, having an immediate effect (pain, inflammation, or hormone imbalance) or lead to a long-term disease (e.g., infertility, chronic obstructive pulmonary disease, or cancer). Toxicological effects have been noticed at high concentrations of MPs, specifically polystyrene, the most widespread typical MP, but only short-term effects have been mostly studied. Significant quantities of consumed MPs have been discovered to have diverse detrimental effects, posing a threat to human welfare. The exact concentrations of microplastics that are inhaled and swallowed and then build up in the human body are still not known. Further investigation is necessary to evaluate the impact of MP/NP contamination at minimal concentrations and for prolonged durations. Full article

(This article belongs to the Collection Exposure and Effects of Environmental Pollution on Vulnerable Populations)

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12 pages, 4531 KB

Open AccessEditor’s ChoiceArticle

Adverse Outcomes Following Exposure to Perfluorooctanesulfonamide (PFOSA) in Larval Zebrafish (Danio rerio): A Neurotoxic and Behavioral Perspective

by Nikita David, Emma Ivantsova, Isaac Konig, Cole D. English, Lev Avidan, Mark Kreychman, Mario L. Rivera, Camilo Escobar, Eliana Maira Agostini Valle, Amany Sultan and Christopher J. Martyniuk

Toxics 2024, 12(10), 723; https://doi.org/10.3390/toxics12100723 - 4 Oct 2024

Cited by 2 | Viewed by 3028

Abstract

Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C₈H₂F₁₇NO₂S) is a common [...] Read more.

Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C₈H₂F₁₇NO₂S) is a common precursor of perfluorooctane sulfonic acid (PFOS), a long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, and distance moved in zebrafish embryos and larvae following continuous exposure to PFOSA beginning at 6 h post-fertilization (hpf). PFOSA decreased survival in fish treated with 1 µg/L PFOSA; however, the effect was modest relative to the controls (difference of 10%). Exposure up to 10 µg/L PFOSA did not affect hatch rate, nor did it induce ROS in 7-day-old larvae fish. The activity of larval fish treated with 100 µg/L PFOSA was reduced relative to the solvent control. Transcripts related to oxidative stress response and apoptosis were measured and BCL2-associated X, apoptosis regulator (bax), cytochrome c, somatic (cycs), catalase (cat), superoxide dismutase 2 (sod2) were induced with high concentrations of PFOSA. Genes related to neurotoxicity were also measured and transcript levels of acetylcholinesterase (ache), elav-like RNA binding protein 3 (elavl3), growth-associated protein 43 (gap43), synapsin II (syn2a), and tubulin 3 (tubb3) were all increased in larval fish with higher PFOSA exposure. These data improve our understanding of the potential sub-lethal toxicity of PFOSA in fish species. Full article

(This article belongs to the Special Issue Impact of Per- and Polyfluoroalkyl Substances (PFAS) on Aquatic Animals: Immunotoxicity, Endocrine Disruption, and Beyond)

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22 pages, 4499 KB

Open AccessEditor’s ChoiceReview

The Application of Nano Zero-Valent Iron in Synergy with White Rot Fungi in Environmental Pollution Control

by Guoming Zeng, Zilong Ma, Rui Zhang, Yu He, Xuanhao Fan, Xiaoling Lei, Yong Xiao, Maolan Zhang and Da Sun

Toxics 2024, 12(10), 721; https://doi.org/10.3390/toxics12100721 - 2 Oct 2024

Cited by 7 | Viewed by 2133

Abstract

Developing efficient and sustainable pollution control technologies has become a research priority in the context of escalating global environmental pollution. Nano zero-valent iron (nZVI), with its high specific surface area and strong reducing power, demonstrates remarkable performance in pollutant removal. Still, its application [...] Read more.

Developing efficient and sustainable pollution control technologies has become a research priority in the context of escalating global environmental pollution. Nano zero-valent iron (nZVI), with its high specific surface area and strong reducing power, demonstrates remarkable performance in pollutant removal. Still, its application is limited by issues such as oxidation, passivation, and particle aggregation. White rot fungi (WRF) possess a unique enzyme system that enables them to degrade a wide range of pollutants effectively, yet they face challenges such as long degradation cycles and low degradation efficiency. Despite the significant role of nZVI in pollutant remediation, most contaminated sites still rely on microbial remediation as a concurrent or ultimate treatment method to achieve remediation goals. The synergistic combination of nZVI and WRF can leverage their respective advantages, thereby enhancing pollution control efficiency. This paper reviews the mechanisms, advantages, and disadvantages of nZVI and WRF in pollution control, lists application examples, and discusses their synergistic application in pollution control, highlighting their potential in pollutant remediation and providing new insights for combined pollutant treatment. However, research on the combined use of nZVI and WRF for pollutant remediation is still relatively scarce, necessitating a deeper understanding of their synergistic potential and further exploration of their cooperative interactions. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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15 pages, 863 KB

Open AccessEditor’s ChoiceReview

Overview of Methylation and Demethylation Mechanisms and Influencing Factors of Mercury in Water

by Wenyu Zhao, Runjie Gan, Bensen Xian, Tong Wu, Guoping Wu, Shixin Huang, Ronghua Wang, Zixuan Liu, Qin Zhang, Shaoyuan Bai, Mingming Fu and Yanan Zhang

Toxics 2024, 12(10), 715; https://doi.org/10.3390/toxics12100715 - 30 Sep 2024

Cited by 10 | Viewed by 4985

Abstract

Mercury, particularly in its methylated form, poses a significant environmental and health risk in aquatic ecosystems. While the toxicity and bioaccumulation of mercury are well documented, there remains a critical gap in our understanding of the mechanisms governing mercury methylation and demethylation in [...] Read more.

Mercury, particularly in its methylated form, poses a significant environmental and health risk in aquatic ecosystems. While the toxicity and bioaccumulation of mercury are well documented, there remains a critical gap in our understanding of the mechanisms governing mercury methylation and demethylation in aquatic environments. This review systematically examines the complex interplay of chemical, biological, and physical factors that influence mercury speciation and transformation in natural water systems. We provide a comprehensive analysis of methylation and demethylation processes, specifically focusing on the dominant role of methanogenic bacteria. Our study highlights the crucial function of hgcAB genes in facilitating mercury methylation by anaerobic microorganisms, an area that represents a frontier in current research. By synthesizing the existing knowledge and identifying key research priorities, this review offers novel insights into the intricate dynamics of mercury cycling in aquatic ecosystems. Our findings provide a theoretical framework to inform future studies and guide pollution management strategies for mercury and its compounds in aquatic environments. Full article

(This article belongs to the Topic The Challenges and Future Trends in Anthropogenic and Natural Pollution Control Engineering)

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18 pages, 12366 KB

Open AccessEditor’s ChoiceArticle

Enhanced Adsorption of Cadmium by a Covalent Organic Framework-Modified Biochar in Aqueous Solution

by Yanwei Hou, Shanna Lin, Jiajun Fan, Youchi Zhang, Guohua Jing and Chao Cai

Toxics 2024, 12(10), 717; https://doi.org/10.3390/toxics12100717 - 30 Sep 2024

Cited by 4 | Viewed by 1915

Abstract

In the environmental field, the advancement of new high-efficiency heavy metal adsorption materials remains a continuous research focus. A novel composite, covalent organic framework-modified biochar (RH-COF), was fabricated via an in-situ polymerization approach in this study. The COF-modified biochar was characterized by elemental [...] Read more.

In the environmental field, the advancement of new high-efficiency heavy metal adsorption materials remains a continuous research focus. A novel composite, covalent organic framework-modified biochar (RH-COF), was fabricated via an in-situ polymerization approach in this study. The COF-modified biochar was characterized by elemental analysis, BET analysis, SEM, FT-IR, and XPS. The nitrogen and oxygen content in the modified material increased significantly from 0.96% and 15.50% to 5.40% and 24.08%, respectively, indicating the addition of a substantial number of nitrogen- and oxygen-containing functional groups to the RH-COF surface, thereby enhancing its adsorption capacity for Cd from 4.20 mg g⁻¹ to 58.62 mg g⁻¹, representing an approximately fourteen-fold increase. Both the pseudo-second-order model and the Langmuir model were suitable for describing the kinetics and isotherms of Cd²⁺ adsorption onto RH-COF. The adsorption performance of Cd²⁺ by RH-COF showed minimal sensitivity to pH values between 4.0 and 8.0, but could be slightly influenced by ionic strength. Mechanistic analysis showed that the Cd²⁺ adsorption on RH-COF was dominated by surface complexation and chelation, alongside electrostatic adsorption, surface precipitation, and Cπ–cation interactions. Overall, these findings suggest that the synthesis of COF-biochar composite may serve as a promising remediation strategy while providing scientific support for applying COF in environmental materials. Full article

(This article belongs to the Special Issue Environmental Transport and Transformation of Pollutants)

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14 pages, 634 KB

Open AccessEditor’s ChoiceArticle

Impact of Skin Decontamination Wipe Solutions on the Percutaneous Absorption of Polycyclic Aromatic Hydrocarbons

by Chandler Probert, R. Bryan Ormond and Ronald E. Baynes

Toxics 2024, 12(10), 716; https://doi.org/10.3390/toxics12100716 - 30 Sep 2024

Cited by 2 | Viewed by 3579

Abstract

Firefighter occupational exposures were categorized as a class 1 (known) carcinogen by the International Agency for Research on Cancer in 2022. As a result, firefighters have become heavily focused on identifying effective and easy to implement decontamination strategies to reduce their chemical exposures. [...] Read more.

Firefighter occupational exposures were categorized as a class 1 (known) carcinogen by the International Agency for Research on Cancer in 2022. As a result, firefighters have become heavily focused on identifying effective and easy to implement decontamination strategies to reduce their chemical exposures. Skin decontamination using wipes post-exposure is one decontamination strategy that every firefighter has available to them. However, firefighters have expressed concerns over the ingredients in the wipe solution increasing dermal absorption. The goal of this study was to determine if the ingredients in skin decontamination wipe solution had any enhancement effect on the dermal absorption of phenanthrene. To determine any enhancement effects, the additive solution of four skin decontamination wipe products was applied to porcine skin 15 min after chemical dosing. The absorption of phenanthrene was tested in vitro using a flow-through diffusion cell system over eight hours. The wipe solution effects on dermal absorption were determined by measuring multiple absorption characteristics including cumulative absorption (µg/cm²), absorption efficiency (% dose absorbed), lag time (minutes), flux (µg/cm²/h), diffusivity (cm²/h), and permeability (cm/h). No penetration enhancement effects were observed in any of the skin decontamination wipe solutions tested; rather, all wipe solutions decreased the absorption of phenanthrene. Slight differences in cumulative absorption among two pairings of skin decontamination wipe solutions, wipes 1 and 3 vs. wipes 2 and 4, were observed, indicating that some ingredients may impact dermal absorption. These findings show that firefighters should continue using skin decontamination wipes to reduce their dermal exposures to fireground contaminants with little concern of increasing the absorption of phenanthrene. Full article

(This article belongs to the Special Issue Firefighters’ Occupational Exposures and Health Risks)

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102 pages, 805 KB

Open AccessEditor’s ChoiceReview

Short-Half-Life Chemicals: Maternal Exposure and Offspring Health Consequences—The Case of Synthetic Phenols, Parabens, and Phthalates

by Delphine Rousseau-Ralliard, Jeanne Bozec, Marion Ouidir, Nicolas Jovanovic, Véronique Gayrard, Namya Mellouk, Marie-Noëlle Dieudonné, Nicole Picard-Hagen, Maria-José Flores-Sanabria, Hélène Jammes, Claire Philippat and Anne Couturier-Tarrade

Toxics 2024, 12(10), 710; https://doi.org/10.3390/toxics12100710 - 29 Sep 2024

Cited by 4 | Viewed by 4027

Abstract

Phenols, parabens, and phthalates (PPPs) are suspected or known endocrine disruptors. They are used in consumer products that pregnant women and their progeny are exposed to daily through the placenta, which could affect offspring health. This review aims to compile data from cohort [...] Read more.

Phenols, parabens, and phthalates (PPPs) are suspected or known endocrine disruptors. They are used in consumer products that pregnant women and their progeny are exposed to daily through the placenta, which could affect offspring health. This review aims to compile data from cohort studies and in vitro and in vivo models to provide a summary regarding placental transfer, fetoplacental development, and the predisposition to adult diseases resulting from maternal exposure to PPPs during the gestational period. In humans, using the concentration of pollutants in maternal urine, and taking the offspring sex into account, positive or negative associations have been observed concerning placental or newborn weight, children’s BMI, blood pressure, gonadal function, or age at puberty. In animal models, without taking sex into account, alterations of placental structure and gene expression linked to hormones or DNA methylation were related to phenol exposure. At the postnatal stage, pollutants affect the bodyweight, the carbohydrate metabolism, the cardiovascular system, gonadal development, the age of puberty, sex/thyroid hormones, and gamete quality, but these effects depend on the age and sex. Future challenges will be to explore the effects of pollutants in mixtures using models and to identify the early signatures of in utero exposure capable of predicting the health trajectory of the offspring. Full article

(This article belongs to the Section Reproductive and Developmental Toxicity)

24 pages, 799 KB

Open AccessEditor’s ChoiceReview

Breast Cancer-Related Chemical Exposures in Firefighters

by Bethsaida Cardona, Kathryn M. Rodgers, Jessica Trowbridge, Heather Buren and Ruthann A. Rudel

Toxics 2024, 12(10), 707; https://doi.org/10.3390/toxics12100707 - 28 Sep 2024

Cited by 3 | Viewed by 4695

Abstract

To fill a research gap on firefighter exposures and breast cancer risk, and guide exposure reduction, we aimed to identify firefighter occupational exposures linked to breast cancer. We conducted a systematic search and review to identify firefighter chemical exposures and then identified the [...] Read more.

To fill a research gap on firefighter exposures and breast cancer risk, and guide exposure reduction, we aimed to identify firefighter occupational exposures linked to breast cancer. We conducted a systematic search and review to identify firefighter chemical exposures and then identified the subset that was associated with breast cancer. To do this, we compared the firefighter exposures with chemicals that have been shown to increase breast cancer risk in epidemiological studies or increase mammary gland tumors in experimental toxicology studies. For each exposure, we assigned a strength of evidence for the association with firefighter occupation and for the association with breast cancer risk. We identified twelve chemicals or chemical groups that were both linked to breast cancer and were firefighter occupational exposures, including polycyclic aromatic hydrocarbons, volatile aromatics, per- and polyfluoroalkyl substances, persistent organohalogens, and halogenated organophosphate flame retardants. Many of these were found at elevated levels in firefighting environments and were statistically significantly higher in firefighters after firefighting or when compared to the general population. Common exposure sources included combustion byproducts, diesel fuel and exhaust, firefighting foams, and flame retardants. Our findings highlight breast-cancer-related chemical exposures in the firefighting profession to guide equitable worker’s compensation policies and exposure reduction. Full article

(This article belongs to the Special Issue Firefighters’ Occupational Exposures and Health Risks)

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22 pages, 499 KB

Open AccessEditor’s ChoiceSystematic Review

Fertilizers and Human Health—A Systematic Review of the Epidemiological Evidence

by Christos F. Tagkas, Evangelos C. Rizos, Georgios Markozannes, Maria A. Karalexi, Lydia Wairegi and Evangelia E. Ntzani

Toxics 2024, 12(10), 694; https://doi.org/10.3390/toxics12100694 - 26 Sep 2024

Cited by 9 | Viewed by 7914

Abstract

Background: Fertilizers are widely used to supply nutrients to crops, thereby increasing yields and soil fertility. However, the effects of their production and application on human health through occupational, residential, and environmental exposure remain unclear. Objective: To conduct a systematic review of epidemiological [...] Read more.

Background: Fertilizers are widely used to supply nutrients to crops, thereby increasing yields and soil fertility. However, the effects of their production and application on human health through occupational, residential, and environmental exposure remain unclear. Objective: To conduct a systematic review of epidemiological studies on the association between exposure to fertilizers and health-related outcomes. Methods: We searched in PubMed, Scopus, and Web of Science for cohort, case-control, cross-sectional, and ecological studies (up to May 2024) related to exposure to fertilizers and any reported human health endpoints across all age groups, without language or geographical limitations. Data were extracted for population and study characteristics, type of fertilizer used, exposure assessment, sample size, outcome and its definition, effect estimate, and quality characteristics from the eligible studies, and they were descriptively synthesized. Results: We found 65 eligible publications, with 407 postulated associations. Forty-six publications (321 associations) assessed exposure to inorganic fertilizers, and nineteen studies (93 associations) assessed organic fertilizers. Exposure assessed was related to occupation, residence, and/or proximity. The assessed outcomes were diverse, with considerable harmonization challenges. Inorganic fertilizers have been associated with an increased risk of cancerous outcomes in a small number of studies with methodological limitations and low replication validity, while organic fertilizers have been associated with infections and diarrhea. Conclusions: The epidemiological evidence suggests possible associations between inorganic fertilizers with solid organ tumors and hematological malignancies and organic fertilizers with infections and diarrhea. However, the available evidence is limited, and heterogeneity prevails. Further research is needed to enlarge the evidence base and increase the replication validity and robustness of the results. Full article

(This article belongs to the Special Issue Occupational and Environmental Exposures, Diseases and Epidemiology: From Basic Research to Human Health)

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17 pages, 2132 KB

Open AccessEditor’s ChoiceArticle

TLR4 as a Potential Target of Me-PFOSA-AcOH Leading to Cardiovascular Diseases: Evidence from NHANES 2013–2018 and Molecular Docking

by Zhilei Mao, Yanling Chen, Haixin Li, Qun Lu and Kun Zhou

Toxics 2024, 12(10), 693; https://doi.org/10.3390/toxics12100693 - 25 Sep 2024

Cited by 8 | Viewed by 2774

Abstract

Background: Concerns have been raised regarding the effects of perfluoroalkyl substance (PFAS) exposure on cardiovascular diseases (CVD), but clear evidence linking PFAS exposure to CVD is lacking, and the mechanism remains unclear. Objectives: To study the association between PFASs and CVD in U.S. [...] Read more.

Background: Concerns have been raised regarding the effects of perfluoroalkyl substance (PFAS) exposure on cardiovascular diseases (CVD), but clear evidence linking PFAS exposure to CVD is lacking, and the mechanism remains unclear. Objectives: To study the association between PFASs and CVD in U.S. population, and to reveal the mechanism of PFASs’ effects on CVD. Methods: To assess the relationships between individual blood serum PFAS levels and the risk of total CVD or its subtypes, multivariable logistic regression analysis and partial least squares discriminant analysis (PLS-DA) were conducted on all participants or subgroups among 3391 adults from the National Health and Nutrition Examination Survey (NHANES). The SuperPred and GeneCards databases were utilized to identify potential targets related to PFAS and CVD, respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of intersection genes were performed using Metascape. Protein interaction networks were generated, and core targets were identified with STRING. Molecular docking was achieved using Autodock Vina 1.1.2. Results: There was a positive association between Me-PFOSA-AcOH and CVD (OR = 1.28, p = 0.022), especially coronary heart disease (CHD) (OR = 1.47, p = 0.007) and heart attack (OR = 1.58, p < 0.001) after adjusting for all potential covariates. Me-PFOSA-AcOH contributed the most to distinguishing between individuals in terms of CVD and non-CVD. Significant moderating effects for Me-PFOSA-AcOH were observed in the subgroup analysis stratified by sex, ethnicity, education level, PIR, BMI, smoking status, physical activity, and hypertension (p < 0.05). The potential intersection targets were mainly enriched in CVD-related pathways, including the inflammatory response, neuroactive ligand–receptor interaction, MAPK signaling pathway, and arachidonic acid metabolism. TLR4 was identified as the core target for the effects of Me-PFOSA-AcOH on CVD. Molecular docking results revealed that the binding energy of Me-PFOSA-AcOH to the TLR4-MD-2 complex was −7.2 kcal/mol, suggesting that Me-PFOSA-AcOH binds well to the TLR4-MD-2 complex. Conclusions: Me-PFOSA-AcOH exposure was significantly associated with CVD. Network toxicology and molecular docking uncovered novel molecular targets, such as TLR4, and identified the inflammatory and metabolic mechanisms underlying Me-PFOSA-AcOH-induced CVD. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

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14 pages, 634 KB

Open AccessEditor’s ChoiceReview

A Review of Toxicological Profile of Fentanyl—A 2024 Update

by Jessica Williamson and Ali Kermanizadeh

Toxics 2024, 12(10), 690; https://doi.org/10.3390/toxics12100690 - 24 Sep 2024

Cited by 6 | Viewed by 12386

Abstract

Fentanyl and its analogues are synthetic opioids of varying potencies that are unfortunately heavily abused. Over the last 15 years, fentanyl and its analogues have contributed to the increasing prominence of hospitalisation and numerous deaths due to drug overdose. In this comprehensive literature [...] Read more.

Fentanyl and its analogues are synthetic opioids of varying potencies that are unfortunately heavily abused. Over the last 15 years, fentanyl and its analogues have contributed to the increasing prominence of hospitalisation and numerous deaths due to drug overdose. In this comprehensive literature review, the mechanism of toxicity of the drug in humans is evaluated. A systematic approach was used whereby the relevant literature has been detailed where the toxicity of fentanyl and/or its analogues to different organs/systems were investigated. Furthermore, the review covers the post-mortem toxicological data and demographic information from past fatal cases where fentanyl was believed to be involved. Such insight into fentanyl toxicity is useful as an aid to better understand the toxic doses of the drug and the suspected mechanism of action and the unexpected complications associated with overdose incidences involving the drug. Finally, the review offers an overview of the traditional and emerging test systems used to investigate the adverse effects of fentanyl on human health. Full article

(This article belongs to the Section Drugs Toxicity)

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15 pages, 16685 KB

Open AccessEditor’s ChoiceArticle

Multi-Scale Meteorological Impact on PM_2.5 Pollution in Tangshan, Northern China

by Qian Liang, Xinxuan Zhang, Yucong Miao and Shuhua Liu

Toxics 2024, 12(9), 685; https://doi.org/10.3390/toxics12090685 - 22 Sep 2024

Cited by 5 | Viewed by 1808

Abstract

Tangshan, a major industrial and agricultural center in northern China, frequently experiences significant PM_2.5 pollution events during winter, impacting its large population. These pollution episodes are influenced by multi–scale meteorological processes, though the complex mechanisms remain not fully understood. This study integrates [...] Read more.

Tangshan, a major industrial and agricultural center in northern China, frequently experiences significant PM_2.5 pollution events during winter, impacting its large population. These pollution episodes are influenced by multi–scale meteorological processes, though the complex mechanisms remain not fully understood. This study integrates surface PM_2.5 concentration data, ground-based and upper–air meteorological observations, and ERA5 reanalysis data from 2015 to 2019 to explore the interactions between local planetary boundary layer (PBL) structures and large-scale atmospheric processes driving PM_2.5 pollution in Tangshan. The results indicate that seasonal variations in PM_2.5 pollution levels are closely linked to changes in PBL thermal stability. During winter, day–to–day increases in PM_2.5 concentrations are often tied to atmospheric warming above 1500 m, as enhanced thermal inversions and reduced PBL heights lead to pollutant accumulation. Regionally, this aloft warming is driven by a high-pressure system at 850 hPa over the southern North China Plain, accompanied by prevailing southwesterly winds. Additionally, southwesterly winds within the PBL can transport pollutants from the adjacent Beijing–Tianjin–Hebei region to Tangshan, worsening pollution. Simulations from the chemical transport model indicate that regional pollutant transport can contribute to approximately half of the near-surface PM_2.5 concentration under the unfavorable synoptic conditions. These findings underscore the importance of multi-scale meteorology in predicting and mitigating severe wintertime PM_2.5 pollution in Tangshan and surrounding regions. Full article

(This article belongs to the Special Issue Atmospheric Aerosols: Source Apportionment, Characterizations, and Detection, 2nd Edition)

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29 pages, 1448 KB

Open AccessEditor’s ChoiceReview

Per- and Polyfluoroalkyl Substances (PFAS) Affect Female Reproductive Health: Epidemiological Evidence and Underlying Mechanisms

by Rui Qu, Jingxuan Wang, Xiaojie Li, Yan Zhang, Tailang Yin and Pan Yang

Toxics 2024, 12(9), 678; https://doi.org/10.3390/toxics12090678 - 18 Sep 2024

Cited by 9 | Viewed by 7761

Abstract

PFAS (per- and polyfluoroalkyl substances) have been extensively used across numerous industries and consumer goods. Due to their high persistence and mobility, they are ubiquitous in the environment. Exposure to PFAS occurs in people via multiple pathways such as dermal contact, water supply, [...] Read more.

PFAS (per- and polyfluoroalkyl substances) have been extensively used across numerous industries and consumer goods. Due to their high persistence and mobility, they are ubiquitous in the environment. Exposure to PFAS occurs in people via multiple pathways such as dermal contact, water supply, air inhalation, and dietary intake. Even if some PFAS are being phased out because of their persistent presence in the environment and harmful impacts on human health, mixes of replacement and legacy PFAS will continue to pollute the ecosystem. Numerous toxicological investigations have revealed harmful effects of PFAS exposure on female reproductive health, e.g., polycystic ovaries syndrome, premature ovarian failure, endometriosis, reproductive system tumors, pregnancy complications, and adverse pregnancy outcomes. Despite extensive epidemiological studies on the reproductive toxicity of PFAS, research findings remain inconsistent, and the underlying mechanisms are not well understood. In this review, we give an in-depth description of the sources and pathways of PFAS, and then review the reproductive toxicity of PFAS and its possible mechanisms. Full article

(This article belongs to the Special Issue Traditional and Emerging Pollutants: Human Exposure Profiles and Health Effects)

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14 pages, 2461 KB

Open AccessEditor’s ChoiceArticle

Fluoride Exposure from Drinking Water Increases the Risk of Stroke: An Ecological Study in Changwu Town, China

by Lin Yuan, Hongna Sun, Yue Li, Zhifeng Xing, Shihui Yin, Fengyu Xie, Jing Zhou, Shuang Li, Liaowei Wu, Wei Huang, Teng Wang, Yanhui Gao, Lijun Zhao and Dianjun Sun

Toxics 2024, 12(9), 679; https://doi.org/10.3390/toxics12090679 - 18 Sep 2024

Cited by 3 | Viewed by 2814

Abstract

Background: Stroke is a major cause of death globally and the leading cause in China. Excessive fluoride exposure has been linked to cardiovascular conditions related to stroke risk factors such as hypertension, atherosclerosis, dyslipidemia, and cardiomyopathy. However, evidence supporting the association between fluoride [...] Read more.

Background: Stroke is a major cause of death globally and the leading cause in China. Excessive fluoride exposure has been linked to cardiovascular conditions related to stroke risk factors such as hypertension, atherosclerosis, dyslipidemia, and cardiomyopathy. However, evidence supporting the association between fluoride exposure and stroke risk is limited. Methods: We constructed an ecological study in Changwu Town, Heilongjiang Province, China, a typical endemic fluorosis area caused by excessive fluoride exposure from drinking water. We collected demographic data, stroke prevalence, and mortality information from 2017 to 2021. Fluoride exposure data were obtained from the national monitoring project on endemic fluorosis. Water fluoride concentrations were measured using the standardized methods. Trend changes in stroke rates were assessed using annual percentage change (APC). Differences in stroke rates among fluoride exposure groups were analyzed using chi-square tests. Results: From 2017 to 2021, the all-ages and age-standardized stroke prevalence rates of permanent residents in Changwu Town increased year by year, while the all-ages and age-standardized mortality rates did not change significantly. The prevalence rates of stroke were significantly higher in endemic fluorosis areas compared to non-endemic areas (p < 0.001). Stratifying the population into tertile groups based on the water fluoride cumulative exposure index (WFCEI) revealed statistically significant differences in stroke prevalence rates (p < 0.001), showing a dose–response relationship with the WFCEI. However, the all-ages and age-standardized mortality rates of stroke were not found to be related to fluoride exposure. Conclusions: Long-term excessive fluoride exposure from drinking water may increase the risk of stroke prevalence, indicating fluoride overexposure as a potential risk factor for stroke. Full article

(This article belongs to the Section Exposome Analysis and Risk Assessment)

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17 pages, 2113 KB

Open AccessEditor’s ChoiceArticle

Occupational Exposure of On-Shift Ottawa Firefighters to Flame Retardants and Polycyclic Aromatic Hydrocarbons

by William Papas, Rocio Aranda-Rodriguez, Xinghua Fan, Cariton Kubwabo, Janet S. L. Lee, Emma Fantin, Elita D. Zheng, Jennifer L. A. Keir, Dave Matschke, Jules M. Blais and Paul A. White

Toxics 2024, 12(9), 677; https://doi.org/10.3390/toxics12090677 - 17 Sep 2024

Cited by 3 | Viewed by 2401

Abstract

Firefighters can be exposed to complex mixtures of airborne substances, including hazardous substances released during structural fires. This study employed silicone wristbands (SWBs) as passive samplers to investigate potential exposure to polycyclic aromatic hydrocarbons (PAHs) and flame retardants (FRs). SWBs were deployed at [...] Read more.

Firefighters can be exposed to complex mixtures of airborne substances, including hazardous substances released during structural fires. This study employed silicone wristbands (SWBs) as passive samplers to investigate potential exposure to polycyclic aromatic hydrocarbons (PAHs) and flame retardants (FRs). SWBs were deployed at different areas of four fire stations, in four truck cabins, and at an office control location; they were also donned outside the jackets of 18 firefighters who responded to fire calls. Overall, office areas had significantly lower PAHs than fire station areas. Vehicle bays and truck cabins had significantly higher concentrations of low molecular weight (LMW) PAHs than sleeping and living room areas. For organophosphate ester flame retardants (OPFRs), tri-n-butyl phosphate (TnBP) and tris(1-chloro-2-propyl) phosphate (TCPP) were detected in all the samples; 2-ethylhexyl diphenyl phosphate (EHDPP) was more frequently detected in the fire station areas. Triphenyl phosphate (TPP) concentrations were highest in the truck cabin and office areas, and tris(1,3-dichloro-2-propyl)phosphate (TDCPP) was highest in truck cabins. Thirteen of 16 PAHs and nine of 36 OPFRs were detected in all the SWBs worn by firefighters, and tris (2-butoxyethyl) phosphate (TBEP) was the predominant OPFR. Levels of LMW PAHs were significantly lower when firefighters did not enter the fire. LMW PAHs, HMW (high molecular weight) PAHs, and EHDPP were significantly elevated when heavy smoke was reported. This work highlights the potential for occupational exposure to PAHs and flame retardants in some fire station areas; moreover, factors that may influence exposure during fire suppression. Whilst firefighters’ occupational exposure to PAHs is likely related to fire suppression and exposure to contaminated gear and trucks, exposure to OPFRs may be more related to their presence in truck interiors and electronics. Full article

(This article belongs to the Special Issue Firefighters’ Occupational Exposures and Health Risks)

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19 pages, 9633 KB

Open AccessEditor’s ChoiceArticle

Paraquat Poisoning: Insights from Autopsy, Histology, and Liquid Chromatography with Tandem Mass Spectrometry in Multidisciplinary Forensic Toxicology Practice

by Issarapong Nuwongsa, Tanyarat Markmee, Nareerat Pholpo, Manoch Chockjamsai, Tawachai Monum, Yutti Amornlertwatana and Preechaya Tajai

Toxics 2024, 12(9), 675; https://doi.org/10.3390/toxics12090675 - 16 Sep 2024

Viewed by 4636

Abstract

The herbicide paraquat (PQ) is responsible for a significant number of fatalities resulting from self-poisoning. Nevertheless, only a limited number of comprehensive studies focusing on fatal PQ poisoning, which include examination of autopsy findings, histopathology, and quantitative analysis of post-mortem samples, have been [...] Read more.

The herbicide paraquat (PQ) is responsible for a significant number of fatalities resulting from self-poisoning. Nevertheless, only a limited number of comprehensive studies focusing on fatal PQ poisoning, which include examination of autopsy findings, histopathology, and quantitative analysis of post-mortem samples, have been published. This study aimed to evaluate autopsy findings, histopathology, and quantitative analysis of PQ in post-mortem human serum samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a simple, sensitive, and specific method. Autopsies were performed on all deaths due to PQ poisoning, and serum samples were sent to the toxicology laboratory for chemical analysis. The method was successfully applied to seven human serum samples, and the results indicate its reliability for detecting PQ. The study reports fatal serum PQ levels ranging from 0.5 to 372.0 µg/mL. The comprehensive data presented in this study can be useful for further research and practical applications. Full article

(This article belongs to the Section Agrochemicals and Food Toxicology)

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22 pages, 2278 KB

Open AccessEditor’s ChoiceReview

Perspectives on the Toxic Effects of Micro- and Nanoplastics on the Environment: A Bibliometric Analysis of the 2014 to 2023 Period

by Xianhong Li and Zhonghong Li

Toxics 2024, 12(9), 676; https://doi.org/10.3390/toxics12090676 - 16 Sep 2024

Cited by 6 | Viewed by 3232

Abstract

Over the past decade, micro- and nanoplastics (MNPs) have garnered significant attention due to their frequent detection in and potential toxic effects on the environment and organisms, making them a serious threat to human health. To comprehensively understand the research on MNPs’ toxicity, [...] Read more.

Over the past decade, micro- and nanoplastics (MNPs) have garnered significant attention due to their frequent detection in and potential toxic effects on the environment and organisms, making them a serious threat to human health. To comprehensively understand the research on MNPs’ toxicity, we employed the R language-based Bibliometrix toolkit (version 4.3.0), VOSviewer (version 1.6.11) and CiteSpace (version 6.3.R1) to perform statistical and visual analyses of 3541 articles pertaining to MNPs’ toxicity between 2014 and 2023, which were retrieved from the Web of Science Core Collection (WOSCC) database. The analysis revealed that research related to MNPs’ toxicity has experienced a rapid increase in recent years. China’s particularly prominent influence in the field of MNPs’ toxicity is evidenced by its academic exchanges and the establishment of a mature cooperation system with other countries (regions), such as the USA and Germany. Studies related to MNPs’ toxicity are primarily published in leading journals, including the Science of the Total Environment, Environmental Pollution, and the Journal of Hazardous Materials. The Chinese Academy of Sciences was identified as the leading institution in terms of research on MNPs’ toxicity, contributing 203 papers to the total number of studies published. Keyword co-occurrence and burst analyses indicated that the current research on MNPs’ toxicity mainly focuses on the toxic effects of MNPs on aquatic organisms, the combined toxicity of MNPs and other contaminants, and the toxic effects and mechanisms of MNPs. Future research should integrate computational toxicology and toxicomics to enhance our understanding of MNPs’ toxicity mechanisms and assess the potential health risks posed by atmospheric MNPs. Full article

(This article belongs to the Section Novel Methods in Toxicology Research)

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15 pages, 1038 KB

Open AccessEditor’s ChoiceArticle

In Vitro Hepatic Clearance Evaluations of Per- and Polyfluoroalkyl Substances (PFAS) across Multiple Structural Categories

by David M. Crizer, Julie R. Rice, Marci G. Smeltz, Katelyn S. Lavrich, Krishna Ravindra, John F. Wambaugh, Michael DeVito and Barbara A. Wetmore

Toxics 2024, 12(9), 672; https://doi.org/10.3390/toxics12090672 - 14 Sep 2024

Cited by 7 | Viewed by 2660

Abstract

Toxicokinetic (TK) assays and in vitro–in vivo extrapolation (IVIVE) models are New Approach Methods (NAMs) used to translate in vitro points of departure to exposure estimates required to reach equivalent blood concentrations. Per- and polyfluoroalkyl substances (PFAS) are a large chemical class with [...] Read more.

Toxicokinetic (TK) assays and in vitro–in vivo extrapolation (IVIVE) models are New Approach Methods (NAMs) used to translate in vitro points of departure to exposure estimates required to reach equivalent blood concentrations. Per- and polyfluoroalkyl substances (PFAS) are a large chemical class with wide-ranging industrial applications for which only limited toxicity data are available for human health evaluation. To address the lack of TK data, a pooled primary human hepatocyte suspension model was used with targeted liquid chromatography–mass spectrometry to investigate substrate depletion for 54 PFAS. A median value of 4.52 μL/(min x million cells) was observed across those that showed significant clearance, with 35 displaying no substrate depletion. Bayesian modeling propagated uncertainty around clearance values for use in IVIVE models. Structural evaluations showed the fluorotelomer carboxylic acids were the only PFAS carboxylates showing appreciable clearance, and per- and polyfluorosulfonamides were more readily metabolized than other PFAS sulfonates. Biotransformation product prediction, using the chemical transformation simulator, suggested hydrolysis of PFAS sulfonamides to more stable sulfonic acids, which is an important consideration for exposure modeling. This effort greatly expands the PFAS in vitro toxicokinetic dataset, enabling refined TK modeling, in silico tool development, and NAM-based human health evaluations across this important set of emerging contaminants. Full article

(This article belongs to the Special Issue PFAS Toxicology and Metabolism)

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11 pages, 744 KB

Open AccessEditor’s ChoiceArticle

The Impact of Ambient Air Pollution on Allergic Rhinitis Symptoms: A Prospective Follow-Up Study

by Wen Sun, Chan Ding, Zhuoying Jiang, Xinliang Zheng, Jinlan Jiang and Huadong Xu

Toxics 2024, 12(9), 663; https://doi.org/10.3390/toxics12090663 - 11 Sep 2024

Cited by 6 | Viewed by 3405

Abstract

Air pollution has become a serious public health problem and there is evidence that air pollution affects the incidence of allergic rhinitis. To further investigate the effect of ambient air pollutants on the severity of allergic rhinitis symptoms, a prospective follow-up study in [...] Read more.

Air pollution has become a serious public health problem and there is evidence that air pollution affects the incidence of allergic rhinitis. To further investigate the effect of ambient air pollutants on the severity of allergic rhinitis symptoms, a prospective follow-up study in patients with allergic rhinitis was conducted. A total of 167 allergic rhinitis patients with a mean age of 35.4 years, who were visiting the hospital, were enrolled. The daily symptom severity of allergic rhinitis and the concentrations of six air pollutants, including PM_2.5, PM₁₀, SO₂, CO, O₃ and NO_2, were collected through follow-up investigations. The impact of ambient air pollutants on symptom severity was assessed via multi-pollutant models. Among several typical ambient air pollutants, we observed correlations of allergic rhinitis symptoms with PM_2.5, PM₁₀, CO, SO₂ and NO₂, whereas O₃ showed no such correlation. Specifically, PM_2.5 and PM₁₀ were significantly associated with sneezing and nasal blockage. NO₂ was significantly correlated with symptoms of rhinorrhea, itchy nose and itchy eyes. CO was significantly linked to sneezing and nasal blockage symptoms. These air pollutants not only had a direct impact on allergic rhinitis symptoms but also exhibited a lagging effect. This study indicates that short-term exposure to air pollutants is associated with exacerbation of nasal symptoms in patients with allergic rhinitis, leading to a decline in their quality of life. Full article

(This article belongs to the Special Issue Health Effects of Exposure to Environmental Pollutants)

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13 pages, 1182 KB

Open AccessEditor’s ChoiceArticle

Recreational Drug Overdose—Clinical Value of Toxicological Analysis

by Tobias Zellner, Florian Eyer, Christian Rabe, Stefanie Geith, Bettina Haberl and Sabrina Schmoll

Toxics 2024, 12(9), 662; https://doi.org/10.3390/toxics12090662 - 10 Sep 2024

Cited by 3 | Viewed by 3454

Abstract

Background: Toxicological analysis of patients with acute recreational drug poisoning can improve our understanding of substance use patterns, clinical symptoms, and improve treatment. Patient history alone may be incomplete or misleading. The objective was to assess the differences in patient history and analytical [...] Read more.

Background: Toxicological analysis of patients with acute recreational drug poisoning can improve our understanding of substance use patterns, clinical symptoms, and improve treatment. Patient history alone may be incomplete or misleading. The objective was to assess the differences in patient history and analytical results, to describe the clinical characteristics, implications and hospital management, and to describe the drug use pattern over time. Methods: A retrospective study including all patients admitted to our toxicology unit with recreational drug toxicity and analytical testing from October 2014 to December 2022. Results: 872 patients were included. Patient history revealed a median of one ingested substance class: opiates/opioids, benzodiazepines/Z-drugs, and Pregabalin were predominant. Urine analysis revealed a median of three ingested substance classes (p < 0.001). Benzodiazepines/Z-drugs, Pregabalin, and THC were severely underreported. Agitation and aggression, anxiety, hallucinations, and psychosis were frequent, associated with cocaine, cathinone/phenethylamine, and amphetamine/MDMA detection and required sedation. Coma was also frequent, associated with opiate/opioid, benzodiazepine/Z-drug, GBL/GHB, and Pregabalin detection and required intubation, and/or application of Naloxone and/or Flumazenil. Twelve patients arrived in cardiac arrest; all were positive for opiates/opioids. Four patients died: three with Benzodiazepines/Z-drugs, Pregabalin and opiates/opioids detected, one with cathinones/phenethylamines detected. While cathinones/phenethylamines and synthetic cannabinoid receptor agonists were mainly detected between 2014–2016, detection decreased significantly between 2017–2022 after NPS legislation passed. Pregabalin detection increased. Conclusions: Patient history is inaccurate, and patients frequently underreport ingested drugs. Opiates and opioids are still the main cause of morbidity and mortality. Pregabalin is increasingly abused. NPS legislation effectively decreased cathinone/phenethylamine and synthetic cannabinoid receptor agonist overdoses. Full article

(This article belongs to the Special Issue The Identification of Narcotic and Psychotropic Drugs)

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21 pages, 1882 KB

Open AccessEditor’s ChoiceReview

A Comprehensive Review of Lab-Scale Studies on Removing Hexavalent Chromium from Aqueous Solutions by Using Unmodified and Modified Waste Biomass as Adsorbents

by Manikant Tripathi, Sukriti Pathak, Ranjan Singh, Pankaj Singh, Pradeep Kumar Singh, Awadhesh Kumar Shukla, Sadanand Maurya, Sukhminderjit Kaur and Babita Thakur

Toxics 2024, 12(9), 657; https://doi.org/10.3390/toxics12090657 - 8 Sep 2024

Cited by 8 | Viewed by 3560

Abstract

Anthropogenic activities and increasing human population has led to one of the major global problems of heavy metal contamination in ecosystems and to the generation of a huge amount of waste material biomass. Hexavalent chromium [Cr(VI)] is the major contaminant introduced by various [...] Read more.

Anthropogenic activities and increasing human population has led to one of the major global problems of heavy metal contamination in ecosystems and to the generation of a huge amount of waste material biomass. Hexavalent chromium [Cr(VI)] is the major contaminant introduced by various industrial effluents and activities into the ecosystem. Cr(VI) is a known mutagen and carcinogen with numerous detrimental effects on the health of humans, plants, and animals, jeopardizing the balance of ecosystems. Therefore, the remediation of such a hazardous toxic metal pollutant from the environment is necessary. Various physical and chemical methods are available for the sequestration of toxic metals. However, adsorption is recognized as a more efficient technology for Cr(VI) remediation. Adsorption by utilizing waste material biomass as adsorbents is a sustainable approach in remediating hazardous pollutants, thus serving the dual purpose of remediating Cr(VI) and exploiting waste material biomass in an eco- friendly manner. Agricultural biomass, industrial residues, forest residues, and food waste are the primary waste material biomass that could be employed, with different strategies, for the efficient sequestration of toxic Cr(VI). This review focuses on the use of diverse waste biomass, such as industrial and agricultural by-products, for the effective remediation of Cr(VI) from aqueous solutions. The review also focuses on the operational conditions that improve Cr(VI) remediation, describes the efficacy of various biomass materials and modifications, and assesses the general sustainability of these approaches to reducing Cr(VI) pollution. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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15 pages, 1217 KB

Open AccessEditor’s ChoiceArticle

Environmental Impact of Waste Treatment and Synchronous Hydrogen Production: Based on Life Cycle Assessment Method

by Yiting Luo and Rongkui Su

Toxics 2024, 12(9), 652; https://doi.org/10.3390/toxics12090652 - 5 Sep 2024

Cited by 12 | Viewed by 2483

Abstract

Based on the life cycle assessment methodology, this study systematically analyzes the energy utilization of environmental waste through photocatalytic treatment and simultaneous hydrogen production. Using 10,000 tons of organic wastewater as the functional unit, the study evaluates the material consumption, energy utilization, and [...] Read more.

Based on the life cycle assessment methodology, this study systematically analyzes the energy utilization of environmental waste through photocatalytic treatment and simultaneous hydrogen production. Using 10,000 tons of organic wastewater as the functional unit, the study evaluates the material consumption, energy utilization, and environmental impact potential of the photocatalytic waste synchronous hydrogen production system (specifically, the synchronous hydrogen production process of 4-NP wastewater with CDs/CdS/CNU). The findings indicate that potential environmental impacts from the photochemical treatment of environmental waste and synchronous hydrogen production primarily manifest in freshwater ecological toxicity, marine ecological toxicity, terrestrial ecological toxicity, and non-carcinogenic toxicity to humans. These ecological impacts stem from the catalyst’s adsorption and metal leaching during the photo-degradation and hydrogen production processes of environmental waste. By implementing reasonable modifications and morphological refinements to the catalyst, these effects can be mitigated while achieving enhanced efficiency in environmental waste processing and simultaneous hydrogen production. The research outcomes provide valuable insights for advancing sustainable development in green technology for environmental waste treatment and energy utilization. Full article

(This article belongs to the Special Issue Biomass Conversion and Organic Waste Utilization in Wastewater)

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20 pages, 1077 KB

Open AccessEditor’s ChoiceReview

Plant Defense Mechanisms against Polycyclic Aromatic Hydrocarbon Contamination: Insights into the Role of Extracellular Vesicles

by Muttiah Barathan, Sook Luan Ng, Yogeswaran Lokanathan, Min Hwei Ng and Jia Xian Law

Toxics 2024, 12(9), 653; https://doi.org/10.3390/toxics12090653 - 5 Sep 2024

Cited by 6 | Viewed by 2618

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants that pose significant environmental and health risks. These compounds originate from both natural phenomena, such as volcanic activity and wildfires, and anthropogenic sources, including vehicular emissions, industrial processes, and fossil fuel combustion. Their classification as [...] Read more.

Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants that pose significant environmental and health risks. These compounds originate from both natural phenomena, such as volcanic activity and wildfires, and anthropogenic sources, including vehicular emissions, industrial processes, and fossil fuel combustion. Their classification as carcinogenic, mutagenic, and teratogenic substances link them to various cancers and health disorders. PAHs are categorized into low-molecular-weight (LMW) and high-molecular-weight (HMW) groups, with HMW PAHs exhibiting greater resistance to degradation and a tendency to accumulate in sediments and biological tissues. Soil serves as a primary reservoir for PAHs, particularly in areas of high emissions, creating substantial risks through ingestion, dermal contact, and inhalation. Coastal and aquatic ecosystems are especially vulnerable due to concentrated human activities, with PAH persistence disrupting microbial communities, inhibiting plant growth, and altering ecosystem functions, potentially leading to biodiversity loss. In plants, PAH contamination manifests as a form of abiotic stress, inducing oxidative stress, cellular damage, and growth inhibition. Plants respond by activating antioxidant defenses and stress-related pathways. A notable aspect of plant defense mechanisms involves plant-derived extracellular vesicles (PDEVs), which are membrane-bound nanoparticles released by plant cells. These PDEVs play a crucial role in enhancing plant resistance to PAHs by facilitating intercellular communication and coordinating defense responses. The interaction between PAHs and PDEVs, while not fully elucidated, suggests a complex interplay of cellular defense mechanisms. PDEVs may contribute to PAH detoxification through pollutant sequestration or by delivering enzymes capable of PAH degradation. Studying PDEVs provides valuable insights into plant stress resilience mechanisms and offers potential new strategies for mitigating PAH-induced stress in plants and ecosystems. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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26 pages, 686 KB

Open AccessEditor’s ChoiceReview

Which Environmental Pollutants Are Toxic to Our Ears?—Evidence of the Ototoxicity of Common Substances

by Gregory M. Zarus, Patricia Ruiz, Rae Benedict, Stephan Brenner, Krystin Carlson, Layna Jeong and Thais C. Morata

Toxics 2024, 12(9), 650; https://doi.org/10.3390/toxics12090650 - 4 Sep 2024

Cited by 6 | Viewed by 5031

Abstract

Ototoxicity refers to the adverse effects of substances on auditory or vestibular functions. This study examines the evidence of ototoxicity’s association with exposure to common environmental pollutants, as documented in toxicological profiles by the Agency for Toxic Substances and Disease Registry. Our aim [...] Read more.

Ototoxicity refers to the adverse effects of substances on auditory or vestibular functions. This study examines the evidence of ototoxicity’s association with exposure to common environmental pollutants, as documented in toxicological profiles by the Agency for Toxic Substances and Disease Registry. Our aim was to evaluate whether the evidence supports modifying the charting of ototoxic effects in the summary tables of these toxicological profiles and providing a guide for scientists to access these data. Health outcomes of interest included hearing loss, vestibular effects, cochlear lesions, tonal alterations, cellular damage, and ototoxicity-related outcomes (neurological, nephrotoxic, hepatic, and developmental effects). We obtained ototoxicity information for 62 substances. Hearing-related effects were reported, along with neurological effects. Overall, 26 profiles reported strong evidence of ototoxicity, including 13 substances previously designated as ototoxic by other health and safety agencies. Commonly studied outcomes included hearing loss, damage to ear anatomy, and auditory dysfunction. Vestibular dysfunction and tinnitus are rarely studied. Our findings highlight the lack of conclusive evidence of ototoxic properties for many substances, especially for pesticides and herbicides. This review supports charting the evidence of ototoxicity separately in toxicological profiles’ summary tables. Improving the communication of ototoxicity-related health effects might impact their recognition and prompt further research. A stronger evidence base could support improved prevention efforts in terms of serious health outcomes. Full article

(This article belongs to the Special Issue Ototoxic Chemical Exposures and Public Health)

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22 pages, 3647 KB

Open AccessEditor’s ChoiceReview

Surfactant-Based Chemical Washing to Remediate Oil-Contaminated Soil: The State of Knowledge

by Yanxin Zhao, Yuhuan Sun, Haihan Sun, Fang Zuo, Shaoping Kuang, Shuwu Zhang and Fayuan Wang

Toxics 2024, 12(9), 648; https://doi.org/10.3390/toxics12090648 - 4 Sep 2024

Cited by 13 | Viewed by 3866

Abstract

As the energy demand increases, there is a significant expansion and utilization of oil resources, resulting in the inevitable occurrence of environmental pollution. Oil has been identified as a prevalent soil contaminant, posing substantial risks to the soil ecosystems. The remediation of soil [...] Read more.

As the energy demand increases, there is a significant expansion and utilization of oil resources, resulting in the inevitable occurrence of environmental pollution. Oil has been identified as a prevalent soil contaminant, posing substantial risks to the soil ecosystems. The remediation of soil contaminated with oil is a formidable undertaking. Increasing evidence shows that chemical washing, a remediation technique employing chemical reagents like surfactants to augment the solubilization, desorption, and separation of petroleum hydrocarbons in soil, proves to be an efficacious approach, but the latest advances on this topic have not been systematically reviewed. Here, we present the state of knowledge about the surfactant-based chemical washing to remediate oil-contaminated soil. Using the latest data, the present article systematically summarizes the advancements on ex situ chemical washing of oil pollution and provides a concise summary of the underlying principles. The use of various surfactants in chemical washing and the factors influencing remediation efficiency are highlighted. Based on the current research status and knowledge gaps, future perspectives are proposed to facilitate chemical washing of oil-polluted soil. This review can help recognize the application of chemical washing in the remediation of oil-polluted soil. Full article

(This article belongs to the Section Toxicity Reduction and Environmental Remediation)

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17 pages, 7943 KB

Open AccessEditor’s ChoiceArticle

Multi-Walled Carbon Nanotubes Accelerate Leukaemia Development in a Mouse Model

by Qingqing Wang, Jingdan Han, Mujia Wei, Huikai Miao, Min Zhang, Biao Wu, Yao Chen, Yanwen Zheng, Robert Peter Gale and Bin Yin

Toxics 2024, 12(9), 646; https://doi.org/10.3390/toxics12090646 - 2 Sep 2024

Cited by 3 | Viewed by 2145

Abstract

Inflammation is associated with an increased risk of developing various cancers in both animals and humans, primarily solid tumors but also myeloproliferative neoplasms (MPNs), myelodysplastic syndromes (MDS), and acute myeloid leukemia (AML). Multi-walled carbon nanotubes (MWCNTs), a type of carbon nanotubes (CNTs) increasingly [...] Read more.

Inflammation is associated with an increased risk of developing various cancers in both animals and humans, primarily solid tumors but also myeloproliferative neoplasms (MPNs), myelodysplastic syndromes (MDS), and acute myeloid leukemia (AML). Multi-walled carbon nanotubes (MWCNTs), a type of carbon nanotubes (CNTs) increasingly used in medical research and other fields, are leading to a rising human exposure. Our study demonstrated that exposing mice to MWCNTs accelerated the progression of spontaneous MOL4070LTR virus-induced leukemia. Additionally, similar exposures elevated pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α and induced reactive oxygen species (ROS) in a murine macrophage cell line. These effects were significantly reduced in immunodeficient mice and when mice were treated with methoxypolyethylene glycol amine (PEG)-modified MWCNTs. These findings underscore the necessity of evaluating the safety of MWCNTs, particularly for those with hematologic cancers. Full article

(This article belongs to the Special Issue Human Exposure to Emerging Contaminants Associated with E-waste Dismantling: Environmental Processes, Human Exposure and Environmental Management)

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23 pages, 9770 KB

Open AccessEditor’s ChoiceArticle

Health Risk Assessment of Heavy Metals in Agricultural Soils Based on Multi-Receptor Modeling Combined with Monte Carlo Simulation

by Yundong Wu, Yan Xia, Li Mu, Wenjie Liu, Qiuying Wang, Tianyan Su, Qiu Yang, Amani Milinga and Yanwei Zhang

Toxics 2024, 12(9), 643; https://doi.org/10.3390/toxics12090643 - 31 Aug 2024

Cited by 14 | Viewed by 3357

Abstract

The spatial characteristics, pollution sources, and risks of soil heavy metals were analyzed on Hainan Island. The results showed that the heavily polluted points accounted for 0.56%, and the number of mildly and above polluted points accounted for 15.27%, respectively, which were mainly [...] Read more.

The spatial characteristics, pollution sources, and risks of soil heavy metals were analyzed on Hainan Island. The results showed that the heavily polluted points accounted for 0.56%, and the number of mildly and above polluted points accounted for 15.27%, respectively, which were mainly distributed in the northern part of the study area. The principal component analysis–absolute principal component score–multiple linear regression (APCS-MLR) and the positive matrix factorization (PMF) revealed four sources of heavy metals: agricultural pollution sources for cadmium, (Cd), industrial and mining pollution sources for arsenic, (As), transportation pollution sources for zinc and lead (Zn and Pb), and natural pollution sources for chromium, nickel, and copper (Cr, Ni, and Cu). The human health risk assessment indicated that the average non-carcinogenic risk (HI) for both adults and children was within the safe threshold (<1), whereas Cr and Ni posed a carcinogenic risk (CR) to human health. In addition, the total non-carcinogenic risk (THI) indicated that heavy metals posed a potential non-carcinogenic risk to children, while the total carcinogenic risk (TCR) remained relatively high, mainly in the northern part of the study area. The results of the Monte Carlo simulation showed that the non-carcinogenic risk (HI) for all heavy metals was <1, but the total non-carcinogenic risk index (THI) for children was >1, indicating a potential health risk above the safe threshold. Meanwhile, nearly 100% and 99.94% of the TCR values exceeded 1 × 10⁻⁴ for children and adults, indicating that Cr and Ni are priority heavy metals for control. The research results provide the necessary scientific basis for the prevention and control of heavy metals in agricultural soils. Full article

(This article belongs to the Collection Artificial Intelligence and Data Mining for Toxicological Sciences)

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16 pages, 2671 KB

Open AccessEditor’s ChoiceArticle

The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation

by Méry Marimoutou, Vivek Patel, Jae Hun Kim, Niccole Schaible, Jose Alvarez, Joseph Hughes, McKenzie Obermok, Carlos Iván Rodríguez, Thomas Kallarakal, Béla Suki, Khalid Amin, Ramaswamy Krishnan and Holger Peter Behrsing

Toxics 2024, 12(9), 637; https://doi.org/10.3390/toxics12090637 - 30 Aug 2024

Cited by 5 | Viewed by 3208

Abstract

Human precision-cut lung slices (hPCLS) prepared from fibrotic lungs recapitulate the pathophysiological hallmarks of fibrosis. These hallmark features can also be induced by treating non-fibrotic hPCLS with a fibrotic cocktail (FC). As a result, the fibrotic and fibrosis-induced hPCLS are rapidly emerging as [...] Read more.

Human precision-cut lung slices (hPCLS) prepared from fibrotic lungs recapitulate the pathophysiological hallmarks of fibrosis. These hallmark features can also be induced by treating non-fibrotic hPCLS with a fibrotic cocktail (FC). As a result, the fibrotic and fibrosis-induced hPCLS are rapidly emerging as preferred models for disease modeling and drug discovery. However, current hPCLS models are limited by tissue viability in culture, as they are usually only viable for one week after harvesting. Here, we demonstrate that the fibrotic hPCLS can be cryopreserved, stored for months, and then thawed on demand without loss of hPCLS viability or protein content for 14 days post-thawing. Cryopreservation also preserves the pro-fibrotic potential of non-fibrotic hPCLS. Specifically, when we treated the thawed non-fibrotic hPCLS with an FC, we observed significant pro-fibrotic cytokine secretion and elevated tissue stiffness. These pro-fibrotic changes were inhibited by the small-molecule tyrosine kinase inhibitor, Nintedanib. Taken together, our work indicates that a feasible solution to prolong the pre-clinical utility of fibrotic and fibrosis-induced hPCLS is cryopreservation. We anticipate that cryopreserved hPCLS will serve as an advantageous predictive model for the evaluation of pro-fibrotic pathways during acute and chronic toxicity testing. Full article

(This article belongs to the Special Issue New Models and Applications in Predictive Toxicology)

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26 pages, 6113 KB

Open AccessEditor’s ChoiceReview

Methods Using Marine Aquatic Photoautotrophs along the Qatari Coastline to Remediate Oil and Gas Industrial Water

by Roda F. Al-Thani and Bassam T. Yasseen

Toxics 2024, 12(9), 625; https://doi.org/10.3390/toxics12090625 - 24 Aug 2024

Cited by 2 | Viewed by 2594

Abstract

Qatar and other Gulf States have a diverse range of marine vegetation that is adapted to the stressful environmental conditions of seawater. The industrial wastewater produced by oil and gas activities adds further detrimental conditions for marine aquatic photosynthetic organisms on the Qatari [...] Read more.

Qatar and other Gulf States have a diverse range of marine vegetation that is adapted to the stressful environmental conditions of seawater. The industrial wastewater produced by oil and gas activities adds further detrimental conditions for marine aquatic photosynthetic organisms on the Qatari coastlines. Thus, these organisms experience severe stress from both seawater and industrial wastewater. This review discusses the biodiversity in seawater around Qatar, as well as remediation methods and metabolic pathways to reduce the negative impacts of heavy metals and petroleum hydrocarbons produced during these activities. The role of microorganisms that are adjacent to or associated with these aquatic marine organisms is discussed. Exudates that are released by plant roots enhance the role of microorganisms to degrade organic pollutants and immobilize heavy metals. Seaweeds may have other roles such as biosorption and nutrient uptake of extra essential elements to avoid or reduce eutrophication in marine environments. Special attention is paid to mangrove forests and their roles in remediating shores polluted by industrial wastewater. Seagrasses (Halodule uninervis, Halophila ovalis, and Thalassia hemprichii) can be used as promising candidates for phytoremediation or bioindicators for pollution status. Some genera among seaweeds that have proven efficient in accumulating the most common heavy metals found in gas activities and biodegradation of petroleum hydrocarbons are discussed. Full article

(This article belongs to the Special Issue Occurrence, Accumulation, and Impacts of Environmental Pollutants in Aquatic Systems)

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25 pages, 2798 KB

Open AccessEditor’s ChoiceReview

Unveiling the Mysteries of Contrast-Induced Acute Kidney Injury: New Horizons in Pathogenesis and Prevention

by Zhong Wang, Qiuhan Wang and Xuezhong Gong

Toxics 2024, 12(8), 620; https://doi.org/10.3390/toxics12080620 - 22 Aug 2024

Cited by 6 | Viewed by 6485

Abstract

The utilization of contrast media (CM) in clinical diagnostic imaging and interventional procedures has escalated, leading to a gradual increase in the incidence of contrast-induced acute kidney injury (CI-AKI). Presently, the scarcity of effective pharmacological treatments for CI-AKI poses significant challenges to clinical [...] Read more.

The utilization of contrast media (CM) in clinical diagnostic imaging and interventional procedures has escalated, leading to a gradual increase in the incidence of contrast-induced acute kidney injury (CI-AKI). Presently, the scarcity of effective pharmacological treatments for CI-AKI poses significant challenges to clinical management. Firstly, we explore the pathogenesis of CI-AKI in this review. Beyond renal medullary ischemia and hypoxia, oxidative stress, cellular apoptosis, and inflammation, emerging mechanisms such as ferroptosis, release of neutrophil extracellular traps (NETs), and nitrosative stress, which offer promising avenues for the management of CI-AKI, are identified. Secondly, a comprehensive strategy for the early prevention of CI-AKI is introduced. Investigating the risk factors associated with CI-AKI is essential for the timely identification of high-risk groups. Additionally, exploring early sensitive biomarkers is crucial for early diagnosis. A synergistic approach that combines these sensitive biomarkers, CI-AKI risk factors, and disease risk prediction models enhances both the accuracy and efficiency of early diagnostic processes. Finally, we explore recent pharmacological and non-pharmacological interventions for the management of Cl-AKI. Beyond the traditional focus on the antioxidant N-acetylcysteine (NAC), we look at active compounds from traditional Chinese medicine, including tetramethylpyrazine (TMP), salvianolic acid B (Sal B), as well as emerging preventive medications like N-acetylcysteine amide (NACA), alprostadil, and others, which all showed potential benefits in animal and clinical studies for CI-AKI prevention. Furthermore, innovative strategies such as calorie restriction (CR), enhanced external counterpulsation (EECP), and mesenchymal stem cell therapy are highlighted as providing fresh insights into Cl-AKI prevention and management. Full article

(This article belongs to the Special Issue Nephrotoxicity Induced by Drugs and Chemicals in the Environment)

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16 pages, 301 KB

Open AccessEditor’s ChoiceArticle

Limitations and Modifications of Skin Sensitization NAMs for Testing Inorganic Nanomaterials

by Britta Wareing, Ayse Aktalay Hippchen, Susanne N. Kolle, Barbara Birk, Dorothee Funk-Weyer and Robert Landsiedel

Toxics 2024, 12(8), 616; https://doi.org/10.3390/toxics12080616 - 21 Aug 2024

Cited by 3 | Viewed by 2242

Abstract

Since 2020, the REACh regulation requires toxicological data on nanoforms of materials, including the assessment of their skin-sensitizing properties. Small molecules’ skin sensitization potential can be assessed by new approach methodologies (NAMs) addressing three key events (KE: protein interaction, activation of dendritic cells, [...] Read more.

Since 2020, the REACh regulation requires toxicological data on nanoforms of materials, including the assessment of their skin-sensitizing properties. Small molecules’ skin sensitization potential can be assessed by new approach methodologies (NAMs) addressing three key events (KE: protein interaction, activation of dendritic cells, and activation of keratinocytes) combined in a defined approach (DA) described in the OECD guideline 497. In the present study, the applicability of three NAMs (DPRA, LuSens, and h-CLAT) to nine materials (eight inorganic nanomaterials (NM) consisting of CeO₂, BaSO₄, TiO₂ or SiO₂, and quartz) was evaluated. The NAMs were technically applicable to NM using a specific sample preparation (NANOGENOTOX dispersion protocol) and method modifications to reduce interaction of NM with the photometric and flowcytometric read-outs. The results of the three assays were combined according to the defined approach described in the OECD guideline No. 497; two of the inorganic NM were identified as skin sensitizers. However, data from animal studies (for ZnO, also human data) indicate no skin sensitization potential. The remaining seven test substances were assessed as “inconclusive” because all inorganic NM were outside the domain of the DPRA, and the achievable test concentrations were not sufficiently high according to the current test guidelines of all three NAMs. The use of these NAMs for (inorganic) NM and the relevance of the results in general are challenged in three ways: (i) NAMs need modification to be applicable to insoluble, inorganic matter; (ii) current test guidelines lack adequate concentration metrics and top concentrations achievable for NM; and (iii) NM may not cause skin sensitization by the same molecular and cellular key events as small organic molecules do; in fact, T-cell-mediated hypersensitivity may not be the most relevant reaction of the immune system to NM. We conclude that the NAMs adopted by OECD test guidelines are currently not a good fit for testing inorganic NM. Full article

(This article belongs to the Special Issue Skin Sensitization Testing Using New Approach Methodologies)

16 pages, 4045 KB

Open AccessEditor’s ChoiceArticle

Impacts of Environmental Concentrations of Nanoplastics on Zebrafish Neurobehavior and Reproductive Toxicity

by Ziqing Sun, Baihui Wu, Jia Yi, Haiyang Yu, Jiaxuan He, Fei Teng, Tong Xi, Jinlong Zhao, Jing Ruan, Peiye Xu, Runchao Tao, Liushuo Jia and Hao Ji

Toxics 2024, 12(8), 617; https://doi.org/10.3390/toxics12080617 - 21 Aug 2024

Cited by 8 | Viewed by 3781

Abstract

Nanoplastics, as emerging environmental pollutants, can transport contaminants across marine environments, polluting pristine ecosystems and being ingested by marine organisms. This transfer poses a severe threat to global aquatic ecosystems and potentially impacts human health through the food chain. Neurobehavioral and reproductive toxicity [...] Read more.

Nanoplastics, as emerging environmental pollutants, can transport contaminants across marine environments, polluting pristine ecosystems and being ingested by marine organisms. This transfer poses a severe threat to global aquatic ecosystems and potentially impacts human health through the food chain. Neurobehavioral and reproductive toxicity are critical areas of concern because they directly affect the survival, health, and population dynamics of aquatic species, which can have cascading effects on the entire ecosystem. Using zebrafish as a model organism, we investigated the toxic effects of environmental concentrations of polystyrene nanoplastics (PS-NPs). Behavioral assessments, including the novel tank test and open field test, demonstrated significant neurobehavioral changes, indicating increased anxiety and depressive behaviors. A pathological analysis of brain and gonadal tissues, along with evaluations of neurobehavioral and reproductive toxicity biomarkers, revealed that exposure to PS-NPs leads to brain tissue lesions, inflammatory responses, oxidative stress activation, hormone level disruptions, and gonadal damage. Real-time quantitative PCR studies of reproductive gene expression further showed that PS-NPs disrupt the endocrine regulation pathways of the brain-pituitary-gonadal (BPG) axis, causing reproductive toxicity with sex-specific differences. These findings provide crucial insights into the impacts of nanoplastics on aquatic organisms and their ecological risks, offering theoretical support for future environmental protection and pollutant management efforts. Full article

(This article belongs to the Special Issue Reproductive and Neurobehavioral Effects of Microplastics on Fish)

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27 pages, 7305 KB

Open AccessEditor’s ChoicePerspective

Current Challenges in Monitoring Low Contaminant Levels of Per- and Polyfluoroalkyl Substances in Water Matrices in the Field

by Hector Medina and Carson Farmer

Toxics 2024, 12(8), 610; https://doi.org/10.3390/toxics12080610 - 20 Aug 2024

Cited by 9 | Viewed by 2690

Abstract

The Environmental Protection Agency (EPA) of the United States recently released the first-ever federal regulation on per- and polyfluoroalkyl substances (PFASs) for drinking water. While this represents an important landmark, it also brings about compliance challenges to the stakeholders in the drinking water [...] Read more.

The Environmental Protection Agency (EPA) of the United States recently released the first-ever federal regulation on per- and polyfluoroalkyl substances (PFASs) for drinking water. While this represents an important landmark, it also brings about compliance challenges to the stakeholders in the drinking water industry as well as concerns to the general public. In this work, we address some of the most important challenges associated with measuring low concentrations of PFASs in drinking water in the field in real drinking water matrices. First, we review the “continuous monitoring for compliance” process laid out by the EPA and some of the associated hurdles. The process requires measuring, with some frequency, low concentrations (e.g., below 2 ppt or 2 ng/L) of targeted PFASs, in the presence of many other co-contaminants and in various conditions. Currently, this task can only (and it is expected to) be accomplished using specific protocols that rely on expensive, specialized, and laboratory-scale instrumentation, which adds time and increases cost. To potentially reduce the burden, portable, high-fidelity, low-cost, real-time PFAS sensors are desirable; however, the path to commercialization of some of the most promising technologies is confronted with many challenges, as well, and they are still at infant stages. Here, we provide insights related to those challenges based on results from ab initio and machine learning studies. These challenges are mainly due to the large amount and diversity of PFAS molecules and their multifunctional behaviors that depend strongly on the conditions of the media. The impetus of this work is to present relevant and timely insights to researchers and developers to accelerate the development of suitable PFAS monitoring systems. In addition, this work attempts to provide water system stakeholders, technicians, and even regulators guidelines to improve their strategies, which could ultimately translate in better services to the public. Full article

(This article belongs to the Section Emerging Contaminants)

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13 pages, 2047 KB

Open AccessEditor’s ChoiceArticle

Perfluoroalkyl and Polyfluoroalkyl Substances in Relation to the Participant-Reported Total Pregnancy and Live Birth Numbers among Reproductive-Aged Women in the United States

by Guangtong Huang, Jiehao Li, Lixin Zhou, Tiantian Duan, Langjing Deng, Pan Yang and Yajie Gong

Toxics 2024, 12(8), 613; https://doi.org/10.3390/toxics12080613 - 20 Aug 2024

Viewed by 2771

Abstract

Perfluoroalkyl and polyfluoroalkyl substances (PFASs), widely utilized in various industries, may pose potential reproductive well-being risks. However, the research on the impact of PFAS exposures on pregnancy and live birth rates remains scarce. To address this gap, we conducted a cross-sectional study using [...] Read more.

Perfluoroalkyl and polyfluoroalkyl substances (PFASs), widely utilized in various industries, may pose potential reproductive well-being risks. However, the research on the impact of PFAS exposures on pregnancy and live birth rates remains scarce. To address this gap, we conducted a cross-sectional study using the data from the United States National Health and Nutrition Examination Survey (NHANES) collected between 2013 and 2018. We focused on six PFAS compounds measured in the serum of women aged 20 to 50 years, employing the Poisson regression, Quantile G-composition (Qgcomp), and Weighted Quantile Sum (WQS) regression models. Adjusting for age, racial/ethnic origin, educational level, marital status, family income, body mass index (BMI), menarche age, birth control pill use, and other female hormone consumption, the Poisson regression identified significant negative associations between the individual PFAS exposures and pregnancy and live birth numbers (p < 0.05 for all 24 null hypotheses for which the slope of the trend line is zero). The Qgcomp analysis indicated that a one-quartile increase in the mixed PFAS exposures was associated with reductions of 0.09 (95% CI: −0.15, −0.03) in the pregnancy numbers and 0.12 (95% CI: −0.19, −0.05) in the live birth numbers. Similarly, the WQS analysis revealed that a unit increase in the WQS index corresponded to decreases of 0.14 (95% CI: −0.20, −0.07) in the pregnancy numbers and 0.14 (95% CI: −0.21, −0.06) in the live birth numbers. Among the six specific PFAS compounds we studied, perfluorononanoic acid (PFNA) had the most negative association with the pregnancy and live birth numbers. In conclusion, our findings suggest that PFAS exposures are associated with lower pregnancy and live birth numbers among women of reproductive age. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

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3 pages, 165 KB

Open AccessEditor’s ChoiceEditorial

Understanding PFAS: Occurrence, Fate, Removal, and Effects

by Chang-Gui Pan and Run-Xia Sun

Toxics 2024, 12(8), 605; https://doi.org/10.3390/toxics12080605 - 19 Aug 2024

Cited by 1 | Viewed by 3021

Abstract

Per- and polyfluoroalkyl substances (PFASs) have emerged as one of the most pressing environmental and public health concerns of the 21st century [...] Full article

(This article belongs to the Special Issue Occurrence, Fate, Removal, and Effects of Per- and Polyfluoroalkyl Substances (PFASs))

13 pages, 4224 KB

Open AccessEditor’s ChoiceArticle

Evaluation of THP-1 and Jurkat Cell Lines Coculture for the In Vitro Assessment of the Effects of Immunosuppressive Substances

by Nina Franko and Marija Sollner Dolenc

Toxics 2024, 12(8), 607; https://doi.org/10.3390/toxics12080607 - 19 Aug 2024

Cited by 1 | Viewed by 3591

Abstract

The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods [...] Read more.

The strong appeal to reduce animal testing calls for the development and validation of in vitro, in chemico and in silico models that would replace the need for in vivo testing and ex vivo materials. A category that requires such new approach methods is the assessment of immunosuppression that can be induced by chemicals including environmental pollutants. To assess the immunosuppressive action on monocytes and lymphocytes, we mimicked the whole-blood cytokine-release assay by preparing an in vitro coculture of THP-1 and Jurkat cell lines. We optimised its activation and investigated the effects of known immunosuppressive drugs with different mechanisms of action on the release of proinflammatory cytokines. Decreased secretion of IL-8 was achieved by several immunosuppressive mechanisms and was therefore selected as an appropriate marker of immunosuppression. A set of environmentally occurring bisphenols, BPA, BPAP, BPP, BPZ, BPE, TCBPA and BPS-MAE, were then applied to the model and BPP and BPZ were found to act as potent immunosuppressants at micromolar concentrations. Full article

(This article belongs to the Special Issue Feature Papers in the Novel Methods in Toxicology Research)

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