Insect-Pathogenic Fungi: Ecology, Evolution, and Applications

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Fungal Pathogens".

Deadline for manuscript submissions: 10 November 2026 | Viewed by 1021

Editors


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Guest Editor
Centro de Estudios Parasitológicos y de Vectores (CONICET- Universidad Nacional de La Plata), La Plata, Provincia de Buenos Aires, Argentina
Interests: pathogenic fungi of insects vectors and of agricultural importance; effect of the cuticle of Blattella germanica (l.) and Periplaneta Fuliginosa (serville) on the pathogenicity of Metarhizium anisopliae (metsch.) sorok. (ascomycota: hypocreales); cockroaches; entomopathogenic fungi; biological control; integrated pest management; insect

E-Mail Website
Guest Editor
Instituto de Investigación de la Cadena Láctea (IDICAL), CONICET-Consejo Nacional de Investigaciones Científicas y Técnicas, INTA-Instituto Nacional de Tecnología Agropecuaria, Rafaela 2300, Santa Fe, Argentina
Interests: conservation biological control; entomophthoralean fungi; non-crop vegetation; cereal crops; lucerne

Special Issue Information

Dear Colleagues,

Fungal pathogens of insects represent a taxonomically diverse and ecologically pivotal group of microorganisms with profound implications for natural and managed ecosystems. This Special Issue aims to advance current knowledge on the biology, ecology, evolution, and applied potential of entomopathogenic fungi, integrating diverse research approaches and lines of investigation.

We welcome contributions addressing host–pathogen interactions, infection mechanisms, virulence determinants, immune evasion strategies, and approaches that elucidate pathogenicity factors. For this Special Issue, particular emphasis is placed on the molecular and ecological bases of host specificity, co-evolutionary dynamics between fungi and their insect hosts, and the environmental drivers shaping disease emergence and transmission.

Given the increasing demand for sustainable pest management strategies, this Special Issue also encourages studies on the development, optimization, and risk assessment of fungal biocontrol agents. Research exploring resistance evolution, formulation technologies, field efficacy, ecological non-target effects, and the broader plant–insect–fungus interactions within trophic and ecological networks is particularly relevant.

By bridging fundamental and applied research, this collection seeks to consolidate current advances and identify future research directions in the study of insect-pathogenic fungi.

Dr. Alejandra Concepción Gutierrez
Dr. Romina G. Manfrino
Guest Editors

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Keywords

  • entomopathogenic fungi
  • host–pathogen interactions
  • pathogenicity factors
  • biological control
  • ecological networks
  • insect immunity

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Published Papers (2 papers)

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Research

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14 pages, 18470 KB  
Article
New Insights into the Larvicidal Activity of Leptolegnia chapmanii Against Aedes aegypti: In Vitro and In Vivo Studies
by Alaine M. L. Catão, Dulcimê Gonçalves Dorta, Walquíria Arruda, Cristian Montalva and Christian Luz
Pathogens 2026, 15(6), 609; https://doi.org/10.3390/pathogens15060609 - 8 Jun 2026
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Abstract
Leptolegnia chapmanii is an oomycete pathogen of mosquito larvae. We investigated whether nutritional factors promoting cyst germination in vitro are associated with early infection events and instar-specific susceptibility in Aedes aegypti. Cysts of the Brazilian isolate ARSEF 12829 germinated rapidly in soybean [...] Read more.
Leptolegnia chapmanii is an oomycete pathogen of mosquito larvae. We investigated whether nutritional factors promoting cyst germination in vitro are associated with early infection events and instar-specific susceptibility in Aedes aegypti. Cysts of the Brazilian isolate ARSEF 12829 germinated rapidly in soybean seed extract, sunflower seed extract and minimal medium supplemented with yeast extract, whereas basal minimal medium did not promote germination. In sunflower seed extract, germination increased significantly with incubation time; in minimal medium, germination at 24 h was much higher with ≥0.2% yeast extract than with 0.1%. In third-instar larvae, a few cysts attached to the cuticle during the first 30–60 min, with no external germ tubes observed. At 3 h, melanized hyphal structures were detected in the midgut, and histological sections showed germinated and ungerminated cysts in the endoperitrophic space, with hyphae crossing the peritrophic matrix and midgut epithelium toward the hemocoel. Mortality increased with cyst concentration and exposure time and decreased with larval instar. At 3.3 × 103 cysts/mL, final mortality reached 100% in L1–L3 and 91.2% in L4 larvae. These results link rapid cyst germination with early midgut invasion and high larvicidal activity. Full article
(This article belongs to the Special Issue Insect-Pathogenic Fungi: Ecology, Evolution, and Applications)
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Review

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49 pages, 4879 KB  
Review
Endophytic Entomopathogenic Fungi Shape Herbivore Behavior and Plant–Insect Interactions: Implications for Biological Control
by Rana H. M. Hussien, Alexandra M. Kortsinoglou, Martyn J. Wood, Vassili N. Kouvelis, Wanissa Mellikeche, Mustapha Touray, Babalwa Tembeni, Mazen Alzain, Faisal Alotaibi, Islam S. Sobhy, Zack Saud, E. Joel Loveridge, Daniel C. Eastwood and Tariq M. Butt
Pathogens 2026, 15(7), 735; https://doi.org/10.3390/pathogens15070735 - 13 Jul 2026
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Abstract
Entomopathogenic fungi (EPF) are well established as biological control agents, but their emerging role as endophytes reveals a broader and more powerful function in crop protection. By colonizing plant tissues, endophytic entomopathogenic fungi (EEPF) create a dynamic tripartite interaction between plants, fungi, and [...] Read more.
Entomopathogenic fungi (EPF) are well established as biological control agents, but their emerging role as endophytes reveals a broader and more powerful function in crop protection. By colonizing plant tissues, endophytic entomopathogenic fungi (EEPF) create a dynamic tripartite interaction between plants, fungi, and herbivores, enabling systemic, plant-mediated pest suppression. This review synthesizes current knowledge on the behavioral and ecological responses of herbivorous arthropods to EEPF-colonized plants, with an emphasis on the mechanisms and implications for integrated pest management (IPM). Growing evidence indicates that EEPF consistently modify herbivore behavior and performance across diverse crops and insect taxa. Colonization frequently alters feeding, host selection, and oviposition, often deterring pests, although mediated responses may vary among fungal species, host plants, insect taxa, and environmental conditions. These responses are driven by EEPF-induced changes in plant chemistry, including shifts in volatile organic compounds (VOCs) and defensive metabolites. In parallel, EEPF impair insect fitness by delaying development, reducing survival, and lowering fecundity, thereby suppressing pest populations. These plant-mediated and behavioral changes extend to multitrophic interactions, potentially affecting associations with natural enemies and the transmission efficiency of some insect vectors of plant viruses. Despite rapid progress, critical gaps remain in resolving the mechanistic basis of these interactions and their stability under field conditions. Advancing the application of EEPF will require integrated approaches combining microbial ecology, chemical ecology, and insect behavioral biology. Harnessing these interactions offers a compelling pathway to reduce reliance on synthetic pesticides while enhancing the resilience and sustainability of agricultural systems. Full article
(This article belongs to the Special Issue Insect-Pathogenic Fungi: Ecology, Evolution, and Applications)
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