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Molecules
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Novel Matrices for MALDI Mass Spectrometry
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Novel Matrices for MALDI Mass Spectrometry

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 September 2022) | Viewed by 6614

Special Issue Editors

Prof. Dr. Antonio Monopoli

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Guest Editor
Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, 4 Via Orabona, I-70126 Bari, Italy
Interests: catalysts for green chemistry; C-C and C-X bond coupling; MALDI mass spectrometry; fuel cells, CO2 and CO chemical fixation
Special Issues, Collections and Topics in MDPI journals
Dr. Cosima Damiana Calvano

E-Mail Website
Guest Editor
Dipartimento di Chimica, Centro Interdipartimentale SMART, Università degli Studi di Bari, Via Orabona, 4-I-70126 Bari, Italy
Interests: MALDI mass spectrometry; liquid chromatography; food safety; lipidomics; food allergies; food recovery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is focused on the synthesis, development and use of novel organic and inorganic matrices in Matrix Assisted Laser Desorption Ionization Mass Spectrometry.

Topics include:

Synthesis and applicability of new matrices including organic, inorganic, and nanostructured ones. Alternative approaches to conventional acidic matrices based on second generation (rationally designed) organic matrices, ionic liquids, and inorganic matrices, including metallic nanoparticles are included.

Development of analytical protocols focused on the utilization of already known organic or inorganic compounds or even their combination as new MALDI matrices;

Rationally designed matrices possessing molecular recognition properties for targeted MALDI MS analysis;

Ionic liquids as extraction tools/signal enhancers in MS analysis of low-abundance molecules or as matrices;

Innovative protocols and matrices for Imaging MALDI-ToF-MS;

Fundamental physo-chemical properties of matrices such as ionization outcomes, promotion of analyte’s ionization/desorption processes, reactivity towards analyte, ionless behaviour, etc.;

Studies specifically dealing with novel matrices applications in food chemistry, medicine, and other fields such as metabolomics, lipidomics and proteomics, materials science, etc. are also welcome.

Prof. Dr. Antonio Monopoli
Dr. Cosima Damiana Calvano
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • MALDI-MS
  • organic chemistry
  • reactive matrices
  • nanomaterials
  • imaging-MALDI-ToF-MS
  • ionic liquids

Published Papers (3 papers)

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Research

13 pages, 2845 KiB  
Article
Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS
by Emanuele Barborini, Giacomo Bertolini, Monica Epifanio, Alexander Yavorskyy, Simone Vinati and Marc Baumann
Molecules 2022, 27(13), 4237; https://doi.org/10.3390/molecules27134237 - 30 Jun 2022
Cited by 1 | Viewed by 1405
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on cluster-assembled super-hydrophilic nanoporous titania films deposited on hydrophobic conductive-polymer substrates feature a unique combination of surface properties that significantly improve the possibilities of capturing and processing biological samples before and during the MALDI-MS analysis without changing [...] Read more.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on cluster-assembled super-hydrophilic nanoporous titania films deposited on hydrophobic conductive-polymer substrates feature a unique combination of surface properties that significantly improve the possibilities of capturing and processing biological samples before and during the MALDI-MS analysis without changing the selected sample target (multi-dimensional MALDI-MS). In contrast to pure hydrophobic surfaces, such films promote a remarkable biologically active film porosity at the nanoscale due to the soft assembling of ultrafine atomic clusters. This unique combination of nanoscale porosity and super-hydrophilicity provides room for effective sample capturing, while the hydrophilic-hydrophobic discontinuity at the border of the dot-patterned film acts as a wettability-driven containment for sample/reagent droplets. In the present work, we evaluate the performance of such advanced surface engineered reactive containments for their benefit in protein sample processing and characterization. We shortly discuss the advantages resulting from the introduction of the described chips in the MALDI-MS workflow in the healthcare/clinical context and in MALDI-MS bioimaging (MALDI-MSI). Full article
(This article belongs to the Special Issue Novel Matrices for MALDI Mass Spectrometry)
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19 pages, 4407 KiB  
Article
Synthesis and Investigation of Novel CHCA-Derived Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Lipids
by Antonio Monopoli, Giovanni Ventura, Andrea Aloia, Fulvio Ciriaco, Angelo Nacci, Tommaso R. I. Cataldi and Cosima D. Calvano
Molecules 2022, 27(8), 2565; https://doi.org/10.3390/molecules27082565 - 15 Apr 2022
Cited by 4 | Viewed by 2264
Abstract
A significant area of study and upgrading for increasing sensitivity and general performances of matrix-assisted laser-desorption ionization (MALDI) mass spectrometry (MS) is related to matrix design. Several efforts have been made to address the challenge of low-mass-region interference-free for metabolomics analysis and specifically [...] Read more.
A significant area of study and upgrading for increasing sensitivity and general performances of matrix-assisted laser-desorption ionization (MALDI) mass spectrometry (MS) is related to matrix design. Several efforts have been made to address the challenge of low-mass-region interference-free for metabolomics analysis and specifically for lipidomics. To this aim, rationally designed matrices as 4-chloro-α-cyanocinnamic acid (ClCCA) were introduced and reported to provide enhanced analytical performances. We have taken this rational design one step further by developing and optimizing new MALDI matrices with a range of modifications on the CHCA core, involving different functionalities and substituents. Of particular interest was the understanding of the electron-withdrawing (e.g., nitro-) or donating (e.g., methoxy-) effects along with the extent of conjugation on the ionization efficiency. In the present work, ten matrices were designed on a reasonable basis, synthesized, and characterized by NMR and UV spectroscopies and laser desorption ionization. With the assistance of these putative MALDI matrices, samples containing phospholipids (PL), and neutral di-/tri-acylglycerols (DAG, TAG) were investigated using milk, fish, blood, and human plasma extracts. In comparison with CHCA and ClCCA, four of them, viz. [(2E,4E)-2-cyano-5-(4-methoxyphenyl)penta-2,4-dienoic acid] (1), [(2E,4E)-2-cyano-5-(4-nitrophenyl)penta-2,4-dienoic acid] (2), [(E)-2-cyano-3-(6-methoxynaphthalen-2-yl)acrylic acid] (6) and [(E)-2-cyano-3-(naphthalen-2-yl)acrylic acid] (7) displayed good to even excellent performances as MALDI matrices in terms of ionization capability, interference-free spectra, S/N ratio, and reproducibility. Especially compound 7 (cyano naphthyl acrylic acid, CNAA) was the election matrix for PL analysis and matrix 2 (cyano nitrophenyl dienoic acid, CNDA) for neutral lipids such as DAG and TAG in positive ion mode. Full article
(This article belongs to the Special Issue Novel Matrices for MALDI Mass Spectrometry)
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13 pages, 2646 KiB  
Article
Important Requirements for the Selection of Internal Standards during the Development of Desorption/Ionization Assays for Drug Quantification in Biological Matrices—A Practical Example
by Margaux Fresnais, Seda Karabulut, Yasmin Abou Zeed, Johannes Ungermann, Julia Benzel, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne and Rémi Longuespée
Molecules 2022, 27(3), 690; https://doi.org/10.3390/molecules27030690 - 21 Jan 2022
Cited by 2 | Viewed by 1831
Abstract
Desorption/ionization mass spectrometry (DI-MS) approaches allow for the rapid quantification of drugs in biological matrices using assays that can be validated according to regulatory guidelines. However, specific adaptations must be applied to create reliable quantification methods, depending on the approach and instrumentation used. [...] Read more.
Desorption/ionization mass spectrometry (DI-MS) approaches allow for the rapid quantification of drugs in biological matrices using assays that can be validated according to regulatory guidelines. However, specific adaptations must be applied to create reliable quantification methods, depending on the approach and instrumentation used. In the present article, we demonstrate the importance of the molecular weight, the fragmentation pattern, and the purity of the internal standard for the development of matrix-assisted laser desorption/ionization (MALDI)-ion mobility (IM)-tandem MS and MS/MS methods. We present preliminary results of method development for the quantification of selinexor in microdialysis fluids with a stable isotopically labeled internal standard. In addition, we discuss the selection of internal standards for MALDI-MS assays using different instrumentations. Full article
(This article belongs to the Special Issue Novel Matrices for MALDI Mass Spectrometry)
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