Macromol

Proteins with additives, especially in small quantities, are of great interest as a subject of study. Machine learning approaches implemented on Raman spectroscopy data could provide an insight into the chemical structures of such mixtures or conjugates. Although decision tree models could be powerful in solving either classification or regression tasks and could provide accessible predictions, they are prone to overfitting. Ensemble models that implement several decision trees could overcome the determined problem. Five different model types are discussed: RandomForest, GradientBoosting, AdaBoost, Voting, and Stacking. Raman spectroscopy data of whey protein isolates (5 wt.%) with different amounts of hyaluronic acid (0, 0.1, 0.25, and 0.5 wt.%) were used as datasets. In order to generalize the results of the study, WPI samples from three different manufacturers were used. Optimization established that ensembles of 200 decision trees with a maximum depth of four were optimal. The Stacking algorithm, which used RandomForest, GradientBoosting, and AdaBoost as base models with either LogisticRegressor (classification task) or RidgeCV (regression task), was found to be the most efficient in finding differences between the whey protein isolate and its conjugates with hyaluronic acid: specificity of 68.7% and sensitivity of 95.4% (classification task); R² = 0.764 with mean absolute error of 0.068 (regression task). According to the feature importance plots, the Raman bands that were most influential in predicting the results were 1003 cm⁻¹ (phenylalanine, ring breath), 1125 cm⁻¹ (rocking of NH₃⁺), 1206 cm⁻¹ (C–C stretching), 1240 cm⁻¹ (amide III (β-sheet), N–H in-plane bend, C–N stretch), and 1399 cm⁻¹ (aspartic and glutamic acids, C=O stretch of COO–). The findings of this study may contribute to the development of novel methods for quality control and analysis of complex multicomponent systems in various industrial settings. In particular, the ensemble approach can be adapted for monitoring in food processing or as a screening tool in pharmaceutical formulation development. Full article

Mushrooms have been used for centuries in traditional folk medicine for the treatment of various diseases and are valued for their health-promoting properties. This long-standing use has sparked growing scientific interest in mushrooms as a source of bioactive compounds. While mushrooms contain a wide range of biologically active substances, including terpenoids, alkaloids, and glycoproteins, this review focuses specifically on polysaccharides derived from mushroom and their potential anti-aging effects at the cellular level. The evidence presented here summarizes current knowledge based on both in vitro and in vivo studies. Additionally, this review highlights the emerging potential of mushroom-derived polysaccharides as natural carriers in advanced drug delivery systems. Although several studies have investigated the use of fungal polysaccharides in combination with therapeutic agents—such as bovine serum albumin, resveratrol, paclitaxel, and quercetin—the potential of combining fungal polysaccharides with senotherapeutics remains unexplored. To fully realize the potential of mushroom-derived polysaccharides in promoting everyday health, combating cellular aging and obtaining synergistic anti-ageing effect via using mushroom polysaccharides as carriers for senolytics, further research is needed. Full article

The linear economic model continues to drive multidimensional environmental problems, as it generates large volumes of plastic waste, as well as agricultural by-products, such as coconut husks. On the other hand, the maritime industry still relies on conventional materials such as wood, steel, and fibre-reinforced plastics, which have several usage challenges, including corrosion, toxicity, and difficulties associated with end-of-life management. These issues point to the need for more sustainable material options. This review examines the potential of combining coconut fibre (coir) with recycled plastics to produce a functional material for use in the maritime sector. The material is designed to add value to waste streams by providing a practical approach to reducing dependence on conventional and less sustainable resources. The review discusses fibre treatments (alkali, silane, acetylation) and fabrication methods (compression moulding, extrusion) and evaluates their impact on mechanical performance and durability. The studies show that coir–plastic composites possess highly tuneable mechanical properties. Tensile strengths are reported to range from approximately 2.4 MPa for natural resin matrices to 78 MPa for polyester hybrids, while the flexural modulus can be increased by up to 99% compared to the neat polymer blend. Fibre treatments (e.g., alkali) and fabrication methods are crucial, as they have been shown to improve tensile and flexural strength by over 40% and impact strength by 150%. However, the composites produced still show vulnerability to water absorption, UV radiation, and biofouling, which could limit their application in marine environments. To this end, several issues require further study, including long-term field validation, enhanced understanding of material fatigue, and scalable manufacturing. Full article

Injectable hydrogels have attracted substantial and rapidly growing interest due to their ability to be administered into cavities of any shape and provide local therapeutic treatment. This study reports the synthesis and characterization of thermosensitive microgels and hydrogels obtained via photoinitiated copolymerization of methacrylated gelatin (GN-MA) and N-isopropylacrylamide (NIPAM) in the absence and presence of N,N′-methylenebisacrylamide (MBA). The effects of monomer concentration, crosslinker content (MBA), and irradiation time on product yield, grafted chain length, and material properties were systematically investigated. Depending on the polymerization conditions, microgel samples exhibited hydrodynamic diameters in the range of 354–1022 nm at 20 °C, which decreased to 183–308 nm upon heating to 40 °C. Freeze-drying of the microgel dispersions resulted in the formation of a porous sponge-like structure with pore sizes of 50–90 µm. Rheological studies of the hydrogel properties demonstrated evident thermoresponsive behavior, with storage moduli (G′) ranging from 20 to 600 Pa, matching the mechanics of certain soft tissues. The hydrogels showed high equilibrium swelling capacity at 20 °C, which was reduced at 40 °C, as well as temperature-dependent moxifloxacin release (38–88% over 6 days) and excellent biocompatibility (>85% cell viability) with human skin fibroblasts. These findings make them promising for biomedical applications such as postoperative cavity filling and local drug delivery. Full article

Lignocellulosic agricultural residues are abundant yet underutilized despite their potential for sustainable bioconversion. This study evaluated spent mushroom substrate (SMS) from Pleurotus ostreatus cultivation and roots of leafy vegetables (RLV) as alternative substrates for Pleurotus production, using wheat straw as a control. Two species, P. ostreatus and P. citrinopileatus, were cultivated on different SMS/RLV ratios and the immunomodulatory potential of harvested mushrooms was assessed. Specifically, protein (PE-D-P3 < 3 kDa) and carbohydrate (CE-D) fractions obtained after in vitro digestion were applied to LPS-challenged THP-1 cells and immune-related gene expression was analyzed by qPCR. Both species significantly modulated immune responses. The PE-D-P3 showed a more pronounced immunomodulatory effect, significantly downregulating IL1B, IL6 and TNF, whereas the CE-D reduced only TNF expression. Substrate composition influenced bioactivity: PE-D-P3 from SMS 80-RLV 20% resulted in the greatest reduction in IL1B, IL6 and TNF, while CE-D from SMS 60-RLV 40% reduced IL1B and CXCL8. These findings provide insights that both fungal species and substrate composition influence immunomodulatory compound production. Valorizing lignocellulosic residues through optimized mushroom cultivation represents a sustainable strategy for producing functional ingredients with applications in human and animal health, particularly for preventing inflammation-related disorders. Full article

The increasing prevalence of drug-resistant microorganisms has prompted research into novel antimicrobial compounds, with 2-thiophene carboxylic acid thiourea derivatives showing promise for future therapeutic applications. However, the poor water solubility of these compounds limits their practical use. This study investigates the formation and characterization of inclusion complexes between 2-hydroxypropyl-β-cyclodextrin (HPβCD) and 2-thiophene carboxylic acid-halogenated (chlorine-, bromine-, and iodine-) thiourea derivatives, seeking to improve their physicochemical properties. The dynamic light scattering (DLS) measurements and UV-Vis spectroscopy provided information related to thiourea–HPβCD aggregates and stoichiometry. Solid-state inclusion compounds and physical mixtures were prepared in two different molar ratios (thioureas:HPβCD = 1:1 and 1:2), and the morphology of the resulting powders was observed by scanning electron microscopy (SEM). Thermogravimetry (TG) and differential scanning calorimetry (DSC) (TG-DSC) coupled analysis were used to analyze thermal profiles in the temperature range of 25 °C to 600 °C, while the spectral data obtained by Fourier transform infrared spectroscopy (FTIR) provided the characteristic vibrational bands of the pure guest molecules and data corresponding to the structural and chemical changes in the host–guest systems. The structural and thermal analyses revealed significant interactions between the host and thioureas molecules, with evidence of possible interactions involving two cyclodextrin molecules. The results demonstrate the presence of intermediate stoichiometry in the inclusion compounds, with possible enhancement of the therapeutic potential of these thiourea derivatives. Full article

Polymer-based dressings constitute an important class of macromolecular biomaterials enabling controlled drug delivery and enhanced wound healing performance. This review summarizes recent advances in the design, fabrication, and functionalization of polymer dressings, with emphasis on natural and synthetic polymer systems applied in biomedical topical and transdermal drug administration. Key material properties, including biocompatibility, mechanical stability, porosity, and degradation behavior, are discussed in relation to drug loading capacity and release kinetics. Current fabrication strategies, such as electrospinning, hydrogel formation, casting, and multilayer assembly, are critically evaluated with respect to structural control and scalability. Particular attention is given to antimicrobial and stimuli-responsive platforms capable of dynamic interaction with the wound microenvironment. Furthermore, challenges related to long-term stability, regulatory requirements, and clinical translation are addressed. By integrating recent experimental findings, this review highlights essential structure–function relationships governing polymer dressing performance and provides design guidelines for next-generation macromolecular topical and transdermal care systems with improved multifunctionality and clinical applicability. Full article

The construction sector is responsible for substantial energy consumption, greenhouse gas emissions, and resource depletion, driving the search for sustainable alternatives to conventional petroleum-based insulation materials. Lignocellulosic biomass, comprising cellulose, hemicellulose, and lignin, offers a renewable resource for the development of bio-based foams with potential application in construction systems. This review provides a comprehensive analysis of bio-based foams tailored to building applications, positioning recent scientific advances against the technical properties of commercial synthetic insulation foams. Key performance parameters, including density, thermal conductivity, compressive strength, dimensional stability, water vapour diffusion resistance, and fire behaviour, are critically examined. Developments in lignocellulosic-based foams are discussed, highlighting processing strategies such as crosslinking, chemical modification, and hybrid reinforcement to enhance mechanical, thermal, and fire performance. The reported results demonstrate that lignin-based polyurethane and phenolic foams can achieve competitive compressive strength and thermal insulation, while cellulose-based aerogels and foams exhibit ultra-low density and promising conductivity values. However, challenges related to moisture sensitivity, fire classification, process scalability, standardisation, and market integration remain significant. Overall, lignocellulosic foams represent a promising pathway toward decarbonised, circular construction systems, provided that technical optimisation and regulatory alignment are successfully achieved. Full article

In this work, an in situ UV-assisted photoreduction was used to functionalize silk fibroin films with silver nanoparticles in order to develop antibacterial devices for wound healing applications. The process showed high efficiency (~80%) in terms of reacted silver precursor. The effects of the silver treatment on fibroin macromolecule were evaluated in function of the process parameters in terms of chemical structure, thermal and mechanical properties, swelling behavior, resistance to degradation and antibacterial activity. Silver functionalization significantly improved the mechanical properties of the films, with Young’s modulus increasing from 0.23 ± 0.04 MPa (methanol-treated samples) to 7.26 ± 0.46 MPa (silver-functionalized samples). In parallel, a marked reduction in swelling degree was observed (from ~360–420% to ~60%), indicating enhanced structural stability. The treated films also exhibited improved resistance to degradation over 7 days under physiological conditions. From a functional perspective, the materials showed strong antibacterial activity, with up to 97–98% reduction in bacterial proliferation for Pseudomonas aeruginosa and Escherichia coli, and up to 93% for methicillin-resistant Staphylococcus aureus. Overall, the results demonstrate that silver functionalization process improves the structural stability of silk fibroin while conferring sustained antibacterial activity, thus supporting their potential application as antimicrobial dressings for the treatment of superficial and low-exudate wounds. Full article

This review analyzes the advances over a five-year period in the development of polymeric sorbents for the purification of aqueous media from key classes of pollutants: hydrocarbons (crude oil, diesel fuel), organic dyes, pharmaceuticals (antibiotics), pesticides, herbicides, volatile organic compounds, and polycyclic aromatic hydrocarbons. Attention is paid to the analysis of structure-property-performance relationships, with an emphasis on comparing materials derived from renewable natural feedstocks (such as cellulose, chitosan, terpenes, vegetable oils, and aloe vera) with synthetic polymers. The analysis reveals that biopolymer-based sorbents exhibit comparable or superior sorption capacities combined with environmental safety, biodegradability, and low cost. The key sorption mechanisms include physical adsorption, hydrophobic interactions, and electrostatic interactions. Despite persisting challenges related to scalability, stability in real-world environments, and the need for efficient regeneration protocols, a convergent approach that combines the advantages of modified natural polymers and functional synthetic components appears to be the most promising strategy for developing cost-effective and sustainable technologies for the restoration of water quality. Full article

Fibroin-based films represent a promising platform for sustainable and bio-derived materials. Existing literature has mainly focused on isolated molecules, plasticizers, or chemical cross-linkers, and the function of complex, multi-component natural extracts as structure-modulating agents in fibroin films remains poorly understood. In this study, edible films containing mulberry leaf extract (MLE; 2–8 wt%) and fibroin (8 wt%) were prepared by solution casting, and their structures were investigated using spectroscopic, morphological, thermal, mechanical, and barrier property analyses. The results reveal that MLE induces concentration-dependent changes in film performance through multicomponent, non-covalent interactions with the fibroin. An approximately 187% increase in tensile strength was achieved at high MLE concentration, confirming effective physical reinforcement. The water vapor transmission rate decreased markedly from 0.888 to 0.170 g·h⁻¹·m⁻², indicating an enhanced moisture barrier, whereas oxygen permeability increased at higher extract loadings, suggesting localized chain rearrangements. High optical transparency in the visible region was maintained (79.95–83.77%), while UV response was selectively altered with extract concentration. Overall, the 8MLE formulation exhibited the most balanced performance. This study demonstrates that plant-derived extracts can serve as effective natural modifiers for tailoring fibroin film properties without inducing crystallization, offering a sustainable strategy for designing bio-based and edible protein film systems. Full article

This study focuses on the development and characterization of advanced composite materials based on poly(ε-caprolactone) (PCL) and poly(vinylidene fluoride) (PVDF), with or without silver nanoparticles (AgNPs), planned for peripheral nerve or bone regeneration. The complementary properties of PCL (biocompatibility and biodegradability) and PVDF (mechanical stability and piezoelectric functionality) were exploited by blending the polymers in different ratios, resulting in binary (PCL/PVDF) and ternary (PCL/PVDF/AgNPs) composites. Green-synthesized AgNPs were integrated to enhance antimicrobial activity and to support tissue repair through improved signal transmission. Functional thin films and electrospun fibres were obtained and subjected to advanced characterization techniques, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermal analysis. The results demonstrated appropriate morphology, chemical composition, structural stability, and favourable interactions with simulated physiological media. Preliminary biocompatibility assays confirmed good cell viability, supporting the biomedical applicability of the designed scaffolds. Overall, the obtained results highlight the potential of AgNPs-functionalized PCL/PVDF binary and ternary composites as promising candidates for flexible, durable, and bioactive implants in peripheral nerve or bone regeneration. Full article

This study developed poly(lactic acid) (PLA) biocomposites reinforced with pine wood flour (10, 20, and 30 wt%) to achieve the interphase through chemical modification. Specifically, the wood flour was treated with poly(propylene glycol) toluene 2,4-diisocyanate terminated (PEGTDI), while 1 wt% poly(lactic acid)-g-maleic anhydride (PLA-g-MA) was integrated as a reactive compatibilizer during extrusion and thermocompression. Fourier-transform infrared spectroscopy (FTIR) analysis corroborated the occurrence of urethane formation and ester/anhydride linkages, as substantiated by the presence of characteristic bands indicative of surface carbamation at 1645 and 1726 cm⁻¹. Thermal analysis revealed that both the pine wood flour and coupling agents promoted PLA crystallization; however, thermogravimetric analysis (TGA) indicated a decrease in thermal stability for functionalized composites, suggesting a trade-off between enhanced interfacial interaction and heat resistance. Mechanical testing demonstrated a significant reinforcement effect, with the Young’s modulus increasing by up to 22% in untreated composites. The coupling agents effectively optimized stress transfer at low fiber loadings (10 wt%), while flexural modulus improvements were predominant at higher loadings (20–30 wt%) regardless of treatment. These findings underscore the criticality of surface modification and compatibilizer selection for tailoring the structural and thermo-mechanical properties of PLA-based biocomposites, thereby providing a pathway for optimized performance in structural applications. Full article

Bio-based, biodegradable, and renewable polymers offer a promising alternative to traditional synthetic polymers derived from petroleum or other non-renewable resources. However, their use is limited by suboptimal properties and high costs. Incorporating sustainable reinforcements into the polymer matrix significantly improves biopolymer performance while preserving key properties, sustainability, and cost-effectiveness. Bio-based polymeric composites have emerged as a crucial category of biopolymers, playing a key role in advancing a sustainable, circular economy. This review provides an updated overview of bio-based polymer composites and nanocomposites, focusing on reinforcement strategies using natural nanofillers and engineered nanoparticles. We summarize key synthesis and processing methods, discuss structure–property relationships, and highlight recent advances in applications such as food packaging, biomedical devices, energy systems, environmental remediation, 3D printing, and supercapacitors. Polymer nanocomposites are versatile, with their performance depending on the type, size, and interactions between the fillers and the polymer matrix. Progress in metallic, ceramic, carbon-based, natural, and hybrid fillers has improved their properties. Using bio-based polymers and renewable fillers supports sustainability. Natural nanofillers derived from renewable sources and industrial byproducts offer a sustainable approach to developing high-performance, biodegradable nanocomposites. Smart nanocomposites can react to external stimuli by integrating specialized fillers that enhance their mechanical and mobility properties. Shape memory nanocomposites can be remotely activated—using heat, electricity, magnets, or light—enabling advanced applications. Finally, we address major challenges and outline future directions for scalable, circular-material solutions, drawing on perspectives from the circular economy and life cycle assessment (LCA). Full article

Bacterial cellulose (BC) has emerged as a structurally robust, biologically compatible, and highly adaptable biomaterial with significant potential for next-generation wound-care technologies. Its nanofibrillar, extracellular-matrix-like architecture provides exceptional moisture retention, mechanical stability, and conformability, enabling BC to function as an active scaffold rather than a traditional dressing. Advances in chemical modification, composite engineering, and bioactive functionalization, including antimicrobial metals, chitosan, biosurfactants, enzymes, and growth factors, have expanded BC’s therapeutic capabilities. Emerging smart BC dressings integrate biosensors, stimuli-responsive drug release, and 3D-printed architectures tailored to patient-specific wound geometries. Parallel developments in artificial intelligence (AI) are transforming BC production by optimizing bioprocessing, guiding genetic engineering, reducing culture media costs, and enabling real-time quality control, thereby improving scalability and industrial feasibility. These combined innovations position BC as a multifunctional, immunologically instructive, and digitally integrated platform for advanced regenerative wound care. This review reframes BC within the contemporary pathophysiology of chronic wounds, emphasizing its roles in immunomodulation, macrophage polarization, angiogenesis, mechanotransduction, and the disruption of mixed bacterial–fungal biofilms that characterize diabetic foot ulcers and other non-healing wounds. BC hydrogels typically contain >90–99% water and exhibit tensile strengths exceeding 200 MPa, enabling robust mechanical performance in wound environments. Advances in BC composites have demonstrated antimicrobial reductions of 3–5 log units against common chronic-wound pathogens. Full article

The production and use of plastics have direct consequences on the environment, such as the greenhouse gas emissions (GHGs) they cause. Therefore, it is necessary to develop materials from renewable sources with a lower environmental impact to replace plastic. In this work, films with bioactive properties have been developed from cellulose nanofibers (CNFs) and natural phenolic compounds for food packaging applications. First, the optimization of the incorporation of three different natural phenolic compounds (tannic acid, p-coumaric acid, and acetosyringone) into nanocellulose was studied using a Box–Behnken design, with the phenols adsorbed by the nanocellulose as the output variable. Once the incorporation was optimized, films containing nanocellulose and phenolic compounds were produced and characterized. Tannic acid showed the best results with regard to the optical properties of the resulting films and achieved a complete blocking of UV-B radiation, as well as adding to nanocellulose antioxidant (4.32 mM TE/g film) and antibacterial capacity (log R of 6.6 ± 0.2 and 3.8 ± 0.1 for Staphylococcus aureus and Escherichia coli, respectively), making these films a promising material for use in contact with food as a packaging material, although more in-depth studies and measures are needed to make these films viable for use in food packaging. Full article

This study investigated the structural and anti-inflammatory properties of Zophobas morio lipids (ZMLs). The fatty acid (FAs) composition showed a higher proportion of unsaturated FAs, mainly consisting of oleic (30.30%) and linoleic acids (20.05%), than saturated FAs, including palmitic (24.80%) and stearic acids (12.96%). In addition, FT-IR and ¹H-NMR analyses confirmed that ZML possessed a typical triglyceride structure, with long-chain alkyl groups. Thermogravimetric analysis (TGA) indicated that ZML exhibited high thermal stability, with a degradation peak at 369 °C. Differential scanning calorimetry (DSC) displayed a thermal transition at −8 °C, corresponding to the crystallization of unsaturated FAs in ZML. ZML significantly inhibits lipopolysaccharide (LPS)-induced pro-inflammatory M1 macrophage polarization by suppressing nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thereby attenuating the expression of inflammatory mediators. Additionally, ZML alleviated inflammatory oxidative stress by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated antioxidant pathway. Notably, ZML not only induced M2 macrophage polarization in quiescent macrophages but also reprogrammed M1 macrophages toward the anti-inflammatory M2 phenotype. These findings suggest that ZML is a natural nutritional lipid source and a potential therapeutic agent for modulating inflammatory response. Full article

This study aimed to prepare and characterize nanoemulsions containing eugenol and 1,8-cineole using the emulsification method and to investigate their anesthetic effects on guppy fish. The optimized formulation comprised a 5–10% mixture of eugenol and 1,8-cineole in a 1:2 ratio, stabilized with 15–20% Tween 80. The selected formulations displayed mean particle sizes below 15 nm, a low polydispersity index (PDI) (<0.5), and a zeta potential that was more negative than −40 millivolts (mV), indicating stable emulsions. Their pH ranged from 6.50 to 6.63, indicating slight acidity. The formulations exhibited non-Newtonian rheology, as well as thinning under shear stress. Three formulations (F2, F6, and F12) remained stable after both accelerated and long-term stability testing. All nanoemulsions were able to induce guppy fish to the third stage of anesthesia. The nanoemulsions with concentrations of 50 mg/L and 100 mg/L eugenol effectively induced sedation and anesthesia in both sexes and reduced the induction and recovery times compared with the ethanol solution. In conclusion, this study highlights nanoemulsions as a promising drug delivery system for alternative anesthetics in aquaculture. Full article

Among the most structurally diverse biomacromolecules, polysaccharides have attracted increased attention as nanocarriers for precision medicine due to their inherent biocompatibility and versatility in functionalization. Molecular features, such as monomer composition, glycosidic linkages, charge density, and chemical modification, essentially determine the nanoscale assembly process of these biopolymers, as well as their biological compatibility. This review highlights the role of these properties in the assembly process of polysaccharide-based nanocarriers leading to a variety of self-assembled nanostructures, such as polyelectrolyte complexes, protein–polysaccharide complexes, amphiphilic micelles, vesicles, hybrid systems, and nanogels, which are extensively discussed throughout the review. This review also focuses on the structure–property–function relationships of nanocarriers as applied to the rapidly developing area of precision medicine, emphasizing the problems of sustainability and reproducibility. By combining the principles of molecular engineering, supramolecular assembly, and measurable properties, this work aims to present a unified view of the molecular engineering of polysaccharide-based nanocarriers for enhanced translation potential, as well as to outline a coherent framework for the rational development of next-generation polysaccharide-based nanocarriers with improved clinical relevance. Full article