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31 pages, 9950 KiB

Open AccessArticle

Effects of Low and High Doses of Deoxynivalenol on Growth Performance, Blood Biochemistry, Histology, Metabolites, and Microbial Community in Adult Rats

by Jinyoung Jeong, Junsik Kim, Boram Lee, Cheolju Park and Minseok Kim

Biology 2025, 14(4), 429; https://doi.org/10.3390/biology14040429 - 16 Apr 2025

Viewed by 371

Abstract

Deoxynivalenol (DON) is a widespread mycotoxin which contaminates several crops, including maize, wheat, and barley. In this study, we investigated the effects of orally administered DON on growth performance, blood biochemistry, histology, the gut microbiome, and metabolism in rats. Six-week-old rats, acclimatized for [...] Read more.

Deoxynivalenol (DON) is a widespread mycotoxin which contaminates several crops, including maize, wheat, and barley. In this study, we investigated the effects of orally administered DON on growth performance, blood biochemistry, histology, the gut microbiome, and metabolism in rats. Six-week-old rats, acclimatized for one week, were subjected to different dietary treatments for 42 days, as follows: CON (control): 0.9% saline; T1: 0.5 ppm DON; T2: 50 ppm DON; and T3: 100 ppm DON. The T3 group had the lowest final body weight (298.5 ± 3.69 g) and average daily gain compared with the control group (338.9 ± 6.43 g, p < 0.05). The feed conversion ratio was highest in the T3 group (4.28 ± 0.28) compared with that in the control group (3.12 ± 0.13, p < 0.05). DON treatment significantly reduced serum levels of creatinine, amylase, urea nitrogen, and alkaline phosphatase, but not alanine aminotransferase. Fibrosis and apoptosis were exacerbated in various tissues with increasing DON concentration. The metabolite profiles of several tissues were significantly different in the DON-treated and control groups. In the cecum, DON treatment increased the abundance of Desulfobacteria, while decreasing that of Firmicutes. Our results indicate that DON levels above the maximum residue limit have serious health consequences for animals. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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23 pages, 5220 KiB

Open AccessArticle

Probiotic Fermentation of Astragalus membranaceus and Raphani Semen Ameliorates Cyclophosphamide-Induced Immunosuppression Through Intestinal Short-Chain Fatty Acid-Dependent or -Independent Regulation of B Cell Function

by Yang Chen, Xiaoqing Wei, Binqi Rui, Yutong Du, Zengjie Lei, Xiujie Guo, Chaoran Wang, Donglin Yuan, Xiuli Wang, Ming Li, Binbin Hou and Yinhui Liu

Biology 2025, 14(3), 312; https://doi.org/10.3390/biology14030312 - 19 Mar 2025

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Abstract

Probiotic fermentation can promote the release of more effective components from traditional Chinese medicines (TCMs). Astragalus membranaceus (Fisch.) Bunge (A. membranaceus) and Raphani Semen are TCMs that have gained attention for their immunoenhancing activities. This study aimed to investigate the effects [...] Read more.

Probiotic fermentation can promote the release of more effective components from traditional Chinese medicines (TCMs). Astragalus membranaceus (Fisch.) Bunge (A. membranaceus) and Raphani Semen are TCMs that have gained attention for their immunoenhancing activities. This study aimed to investigate the effects and underlying mechanisms of probiotic-fermented A. membranaceus and Raphani Semen (PROAS) in cyclophosphamide (CTX)-induced immunocompromised mice. Changes in the composition of A. membranaceus and Raphani Semen after fermentation by probiotic strains, including Bifidobacterium longum SD5219, Lactobacillus fermentum NCIMB5221, and Lactobacillus paracasei SD5219, were identified using high-performance liquid chromatography. The immunostimulatory effects and mechanisms of PROAS were evaluated in immunosuppressed mice 3 and 7 days after CTX treatment. Probiotic fermentation of TCMs resulted in changes in major bioactive components. PROAS supplementation effectively restored intestinal integrity in CTX-treated mice by upregulating the mRNA expression of the tight junction proteins. PROAS significantly ameliorated the reduction in the spleen index and number of B lymphocytes caused by CTX treatment and regulated the secretion of cytokines in serum and colon tissues. PROAS administration modulated gut microbial dysbiosis and short-chain fatty acid (SCFA) content in CTX-treated mice. These results suggest that PROAS enhances B lymphocyte function by increasing the regulation of intestinal microbiota to produce high levels of SCFA, repairs the intestinal barrier damage induced by CTX, and promotes intestinal mucosal immunity. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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15 pages, 2854 KiB

Open AccessArticle

Gut Microbiota Alteration with Moderate-to-Vigorous-Intensity Exercise in Middle School Female Football Athletes

by Jianlou Yang, Wei Zhang and Chen Dong

Biology 2025, 14(2), 211; https://doi.org/10.3390/biology14020211 - 17 Feb 2025

Viewed by 646

Abstract

Background: The gut microbiota significantly influences health and metabolic processes. This study aimed to investigate the impact of exercise intensity on the gut microbiota of middle school female football athletes. Methods: In this four-week controlled comparative study, twenty-nine participants were divided into three [...] Read more.

Background: The gut microbiota significantly influences health and metabolic processes. This study aimed to investigate the impact of exercise intensity on the gut microbiota of middle school female football athletes. Methods: In this four-week controlled comparative study, twenty-nine participants were divided into three groups: non-exercise group (NEG), moderate-intensity exercise group (MIEG), and vigorous-intensity exercise group (VIEG). They followed their respective exercise regimens for four weeks. Fecal samples were collected for 16S rRNA gene sequencing to evaluate microbiota composition. Results: The MIEG exhibited significantly greater microbial diversity compared to the NEG, while the VIEG showed lower diversity than the MIEG. Various microbiota profiles were identified, with the MIEG having higher levels of beneficial bacteria such as Bacteroides. Conclusions: Moderate-intensity exercise promotes a healthier gut microbiota compared to vigorous exercise in young female athletes. These findings underscore the potential of moderate exercise to enhance gut health and may inform training strategies for adolescent athletes. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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25 pages, 3518 KiB

Open AccessArticle

Macro, Trace and Toxic Element Composition in Liver and Meat of Broiler Chicken Associated with Cecal Microbiome Community

by Dmitry Deryabin, Dianna Kosyan, Ludmila Vlasenko, Christina Lazebnik, Alexander Zatevalov, Ilshat Karimov and Galimzhan Duskaev

Biology 2024, 13(12), 975; https://doi.org/10.3390/biology13120975 - 26 Nov 2024

Viewed by 823

Abstract

The current study presents a meta-analysis of the detailed relationship between the composition of 25 essential and toxic elements in chicken tissues examined by ICP-MS and the gut microbial community analyzed using NGS techniques. The examination of chicken liver and meat revealed typical [...] Read more.

The current study presents a meta-analysis of the detailed relationship between the composition of 25 essential and toxic elements in chicken tissues examined by ICP-MS and the gut microbial community analyzed using NGS techniques. The examination of chicken liver and meat revealed typical elemental compositions, called the “elementomes”. The α-elementomes showed high contents of macro elements (Na, K, Mg, Ca, P), majority trace elements (Sr, Se, Mn, Fe, Co, Cu, Zn) and some toxic elements (B, Pb, Ni, Cd); β-elementomes indicated accumulation of Si, V and Cr; γ-elementomes indicated accumulation of Al, As and Hg. Characterization of the microbiomes’ structure showed two distinct enterotypes, designated “microbiome patterns”; the first was enriched in the phylum Bacteroidota, and the second was dominated by Bacillota and coupled with members of the phyla Actinomycetota, Cyanobacteriota and Thermodesulfobacteriota. A comparison of elementomes and microbiomes demonstrated a clear correspondence between the α- and γ-elementomes belonging to the Bacteroidota-enriched pattern, while the β-elementome was predominantly found in chicken groups belonging to the Bacillota + ACT pattern. This insight proposes a novel strategy to improve deficiency or excess of certain elements in the host by gut microbiome modulation, which needs to be verified with further in vivo experiments. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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15 pages, 6895 KiB

Open AccessEditor’s ChoiceArticle

Curcumin Mitigates Gut Dysbiosis and Enhances Gut Barrier Function to Alleviate Metabolic Dysfunction in Obese, Aged Mice

by Gopal Lamichhane, Femi Olawale, Jing Liu, Da-Yeon Lee, Su-Jeong Lee, Nathan Chaffin, Sanmi Alake, Edralin A. Lucas, Guolong Zhang, Josephine M. Egan and Yoo Kim

Biology 2024, 13(12), 955; https://doi.org/10.3390/biology13120955 - 21 Nov 2024

Cited by 1 | Viewed by 2489

Abstract

The gut microbiome plays a critical role in maintaining gut and metabolic health, and its composition is often altered by aging and obesity. This study aimed to investigate the protective effects of curcumin on gut dysbiosis, gut barrier integrity, and bile acid homeostasis [...] Read more.

The gut microbiome plays a critical role in maintaining gut and metabolic health, and its composition is often altered by aging and obesity. This study aimed to investigate the protective effects of curcumin on gut dysbiosis, gut barrier integrity, and bile acid homeostasis in aged mice fed a high-fat, high-sugar diet (HFHSD). Eighteen- to twenty-one-month-old male C57BL/6 mice were divided into groups fed a normal chow diet or HFHSD, with or without curcumin supplementation (0.4% w/w) for 8 and 15 weeks. We assessed body weight, food intake, insulin sensitivity, gut microbiota composition, and gene expression in the gut and liver and performed histological analysis of gut tissues. Curcumin supplementation prevented HFHSD-induced weight gain and metabolic disturbances. In the gut, curcumin-treated mice showed a higher abundance of beneficial bacterial genera, such as Lachnospiraceae, Akkermansia, Mucispirillum, and Verrucomicrobiota, alongside a lower abundance of harmful bacterial genera like Desulfobacteria, Alistipes, and Muribaculaceae compared to control. This shift in gut microbiota was associated with improved gut integrity, as demonstrated by increased expression of the tight junction protein occludin and reduced levels of the pro-inflammatory marker interleukin-1β in the ileum. Additionally, curcumin modulated hepatic gene expression involved in bile acid homeostasis, suggesting a positive effect on liver health. Curcumin supplementation can alleviate the negative effects of aging and an HFHSD on the gut microbiome, improve gut barrier integrity, and maintain bile acid homeostasis. These findings highlight curcumin’s potential as a dietary intervention for managing obesity- and age-associated gut health issues. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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16 pages, 7702 KiB

Open AccessArticle

Lactiplantibacillus plantarum 1008 Enhances Testicular Function and Spermatogenesis via the Modulation of Gut Microbiota in Male Mice with High-Fat-Diet-Induced Obesity

by Chin-Yu Liu, Yi-Wen Chen, Tsung-Yu Tsai, Te-Hua Liu, Ting-Chia Chang and Chih-Wei Tsao

Biology 2024, 13(11), 890; https://doi.org/10.3390/biology13110890 - 31 Oct 2024

Viewed by 1461

Abstract

Our study was designed to investigate the Lactiplantibacillus plantarum 1008 (LP1008) on testicular antioxidant capacity, spermatogenesis, apoptosis, autophagy, and metabolic function in male mice with high-fat-diet-induced obesity. A total of thirty-six male C57BL/6 mice were fed a normal diet (denoted as the NC [...] Read more.

Our study was designed to investigate the Lactiplantibacillus plantarum 1008 (LP1008) on testicular antioxidant capacity, spermatogenesis, apoptosis, autophagy, and metabolic function in male mice with high-fat-diet-induced obesity. A total of thirty-six male C57BL/6 mice were fed a normal diet (denoted as the NC group) or a high-fat control diet (denoted as the HFC group) for 16 weeks, then half of the HFC group was randomly chosen and subsequently fed with LP1008 for the final 8 weeks (high-fat diet + LP1008; denoted as the HFP group). The HFP group expressed improved blood cholesterol, insulin resistance, hepatic function, and lipopolysaccharide (LPS) levels compared to the HFC group. Meanwhile, the HFC group displayed decreased testicular testosterone levels, sperm quality, and 17β-HSD protein expression, which were rescued after LP1008 treatment. Moreover, the HFC group had lower superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) enzyme activities. After LP1008 treatment, enhanced antioxidative activities and decreased lipid peroxidation were observed. The HFC group also exhibited aggravated apoptosis, inflammation, and autophagy proteins in the testis, which were ameliorated by LP1008 supplementation. Furthermore, the gut microbiota analysis results revealed that the Firmicutes/Bacteroidetes ratio was significantly elevated in the HFC and HFP groups compared to the NC group and that LP1008 treatment diminished Ruminococcaceae and enhanced Bifidobacteriaceae diversity. In summary, LP1008 treatment strengthened antioxidative enzyme levels and regulated microbiota-ameliorated HFC-induced oxidative stress, apoptosis, inflammation, and autophagy, and thus improved testicular function and semen quality. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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19 pages, 5506 KiB

Open AccessArticle

Efficacy of High-Dose Synbiotic Additives for Deoxynivalenol Detoxification: Effects on Blood Biochemistry, Histology, and Intestinal Microbiome in Weaned Piglets

by Jin-Young Jeong, Junsik Kim, Minji Kim and Sungkwon Park

Biology 2024, 13(11), 889; https://doi.org/10.3390/biology13110889 - 31 Oct 2024

Cited by 1 | Viewed by 1238

Abstract

Deoxynivalenol (DON) is a common mycotoxin observed in cereal grains, and feed contamination poses health risks to pigs. Biological antidotes, such as synbiotics (SYNs), have garnered attention for mitigating DON toxicity. This study aimed to assess the efficacy of SYNs by comparing the [...] Read more.

Deoxynivalenol (DON) is a common mycotoxin observed in cereal grains, and feed contamination poses health risks to pigs. Biological antidotes, such as synbiotics (SYNs), have garnered attention for mitigating DON toxicity. This study aimed to assess the efficacy of SYNs by comparing the blood biochemistry, histology, and gut microbiome of weaned piglets. A 4-week trial was conducted on 32 weaned piglets. After a week of diet and environmental adaptation, the pigs were divided into four groups: (1) control (CON, n = 8); (2) SYN (n = 8); (3) DON (n = 8); and (4) DON+SYN (n = 8). The SYN supplementation of weaned piglets increased the final body weight (21.71 ± 0.93 vs. 20.73 ± 0.84), average daily gain (0.38 ± 0.02 vs. 0.34 ± 0.02), and gain-to-feed ratio (0.49 ± 0.04 vs. 0.43 ± 0.02), and decreased the feed conversion ratio (2.14 ± 0.14 vs. 2.39 ± 0.13) compared to the DON group. A high dose of DON induced liver and colon fibrosis and liver and cecum apoptosis, which were alleviated by SYNs. Glucose in the DON group (84.9 ± 3.7) was significantly lower than in the control (101.3 ± 4.2). Additionally, both the DON and DON+SYN groups exhibited higher creatine (0.9 ± 0.0 and 0.9 ± 0.1) and lower cholesterol (88.3 ± 3.2 and 90.0 ± 4.8) levels (p < 0.05). In conclusion, SYNs alleviated DON toxicity, indicating its potential as an antidote for specific biomarkers. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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19 pages, 4408 KiB

Open AccessArticle

Effects of Increasing Oral Deoxynivalenol Gavage on Growth Performance, Blood Biochemistry, Metabolism, Histology, and Microbiome in Rats

by Jin-Young Jeong, Junsik Kim, Minji Kim, Seong-Hoon Shim, Cheolju Park, Sungju Jung and Hyunjung Jung

Biology 2024, 13(10), 836; https://doi.org/10.3390/biology13100836 - 18 Oct 2024

Cited by 2 | Viewed by 1277

Abstract

Mycotoxin-contaminated feed or food can affect physiological responses and cause illnesses in humans and animals. In this study, we evaluated the effects of deoxynivalenol (DON) toxicity on the growth performance, blood biochemistry, histology, microbiome, and metabolism of rats fed with different toxin concentrations. [...] Read more.

Mycotoxin-contaminated feed or food can affect physiological responses and cause illnesses in humans and animals. In this study, we evaluated the effects of deoxynivalenol (DON) toxicity on the growth performance, blood biochemistry, histology, microbiome, and metabolism of rats fed with different toxin concentrations. After 1 week of acclimatization, seven-week-old male rats received 0.9% saline as a control, 0.02 mg/kg DON as T1, and 0.2 mg/kg DON as T2 via oral gavage for 4 weeks. The final body weight of the T2 group was significantly lower than that of the control and T1; however, the average daily gain, feed intake, and feed conversion ratio did not differ. Fibrosis and apoptosis were observed in various tissues as DON concentration increased. Creatinine and alkaline phosphatase levels were significantly lower in the DON-treated group than in the control. Firmicutes and Desulfobacterota phyla dominated the cecum, whereas those in the feces were Proteobacteria and Bacteroidetes. Metabolomic profiling showed phenylalanine, tyrosine, and tryptophan biosynthesis as the most prominent pathways. Overall, our results suggest that low-dose and short-term DON exposure can trigger several adverse effects in rats. Dietary toxicants in rats may explain the physiological effects associated with the metabolism commonly reported in animals. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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16 pages, 2184 KiB

Open AccessArticle

Association between the Maternal Gut Microbiome and Macrosomia

by Zixin Zhong, Rongjing An, Shujuan Ma, Na Zhang, Xian Zhang, Lizhang Chen, Xinrui Wu, Huijun Lin, Tianyu Xiang, Hongzhuan Tan and Mengshi Chen

Biology 2024, 13(8), 570; https://doi.org/10.3390/biology13080570 - 28 Jul 2024

Cited by 1 | Viewed by 1652

Abstract

Fetal macrosomia is defined as a birthweight ≥4000 g and causes harm to pregnant women and fetuses. Studies reported that the maternal intestinal microbiome plays a key role in the establishment, growth, and development of the fetal intestinal microbiome. However, whether there is [...] Read more.

Fetal macrosomia is defined as a birthweight ≥4000 g and causes harm to pregnant women and fetuses. Studies reported that the maternal intestinal microbiome plays a key role in the establishment, growth, and development of the fetal intestinal microbiome. However, whether there is a relationship between maternal gut microbiota and macrosomia remains unclear. Our study aimed to identify gut microbiota that may be related to the occurrence of macrosomia, explore the possible mechanisms by which it causes macrosomia, and establish a prediction model to determine the feasibility of predicting macrosomia by early maternal gut microbiota. We conducted a nested case-control study based on an early pregnancy cohort (ChiCTR1900020652) in the Maternity and Child Health Hospital of Hunan Province on fecal samples of 93 women (31 delivered macrosomia as the case group and 62 delivered normal birth weight newborns as the control group) collected and included in this study. We performed metagenomic analysis to compare the composition and function of the gut microbiome between cases and controls. Correlation analysis was used to explore the association of differential species and differential functional pathways. A random forest model was used to construct an early pregnancy prediction model for macrosomia. At the species level, there were more Bacteroides salyersiae, Bacteroides plebeius, Ruminococcus lactaris, and Bacteroides ovatus in the intestinal microbiome of macrosomias’ mothers compared with mothers bearing fetuses that had normal birth weight. Functional pathways of the gut microbiome including gondoate biosynthesis, L-histidine degradation III, cis-vaccenate biosynthesis, L-arginine biosynthesis III, tRNA processing, and mannitol cycle, which were more abundant in the macrosomia group. Significant correlations were found between species and functional pathways. Bacteroides plebeius was significantly associated with the pathway of cis-vaccenate biosynthesis (r = 0.28, p = 0.005) and gondoate biosynthesis (r = 0.28, p < 0.001) and Bacteroides ovatus was positively associated with the pathway of cis-vaccenate biosynthesis (r = 0.29, p = 0.005) and gondoate biosynthesis (r = 0.32, p = 0.002). Bacteroides salyersiae was significantly associated with the pathway of cis-vaccenate biosynthesis (r = 0.24, p = 0.018), gondoate biosynthesis (r = 0.31, p = 0.003), and L–histidine degradation III (r = 0.22, p = 0.291). Finally, four differential species and four clinical indicators were included in the random forest model for predicting macrosomia. The areas under the working characteristic curves of the training and validation sets were 0.935 (95% CI: 0.851~0.979) and 0.909 (95% CI: 0.679~0.992), respectively. Maternal gut microbiota in early pregnancy may play an important role in the development of macrosomia and can be used as potential predictors to prevent macrosomia. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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22 pages, 7850 KiB

Open AccessArticle

Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish

by Yajuan Huang, Yadong Chen, Haisheng Xie, Yidong Feng, Songlin Chen and Baolong Bao

Biology 2024, 13(6), 372; https://doi.org/10.3390/biology13060372 - 23 May 2024

Cited by 2 | Viewed by 2065

Abstract

The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as a model to investigate the effects of nos2a/nos2b [...] Read more.

The gut microbiota constitutes a complex ecosystem that has an important impact on host health. In this study, genetically engineered zebrafish with inducible nitric oxide synthase (iNOS or NOS2) knockout were used as a model to investigate the effects of nos2a/nos2b gene single knockout and nos2 gene double knockout on intestinal microbiome composition and function. Extensive 16S rRNA sequencing revealed substantial changes in microbial diversity and specific taxonomic abundances, yet it did not affect the functional structure of the intestinal tissues. Notably, iNOS-deficient zebrafish demonstrated a decrease in Vibrio species and an increase in Aeromonas species, with more pronounced effects observed in double knockouts. Further transcriptomic analysis of the gut in double iNOS knockout zebrafish indicated significant alterations in immune-related and metabolic pathways, including the complement and PPAR signaling pathways. These findings underscore the crucial interplay between host genetics and gut microbiota, indicating that iNOS plays a key role in modulating the gut microbial ecology, host immune system, and metabolic responses. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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23 pages, 5422 KiB

Open AccessArticle

Effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the Structure and Function of the Intestinal Flora in Rabbits Undergoing Hepatic Artery Infusion Chemotherapy

by Xiangdong Yan, Liuhui Bai, Jin Lv, Ping Qi, Xiaojing Song and Lei Zhang

Biology 2024, 13(5), 327; https://doi.org/10.3390/biology13050327 - 8 May 2024

Cited by 1 | Viewed by 1874

Abstract

Few studies have explored the biological mechanism by which probiotics alleviate adverse reactions to chemotherapy drugs after local hepatic chemotherapy perfusion by regulating the intestinal flora. This study investigates the effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus [...] Read more.

Few studies have explored the biological mechanism by which probiotics alleviate adverse reactions to chemotherapy drugs after local hepatic chemotherapy perfusion by regulating the intestinal flora. This study investigates the effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the intestinal microbial structure and intestinal barrier function, as well as the potential mechanism in rabbits after local hepatic chemotherapy infusion. Eighteen New Zealand White rabbits were randomly divided into a control group, a hepatic local chemotherapy perfusion group, and a hepatic local chemotherapy perfusion + Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets group to assess the effects of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets on the adverse reactions. The administration of Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets alleviated the intestinal flora disorder caused by local hepatic perfusion chemotherapy, promoted the growth of beneficial bacteria, and inhibited the growth of harmful bacteria. The Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets also reduced the levels of serum pro-inflammatory cytokines and liver injury factors induced by local hepatic perfusion chemotherapy. Our findings indicate that Combined Live Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus Cereus Tablets can ameliorate the toxicity and side effects of chemotherapy by regulating intestinal flora, blocking pro-inflammatory cytokines, reducing liver injury factors, and repairing the intestinal barrier. Probiotics may be used as a potential alternative therapeutic strategy to prevent the adverse reactions caused by chemotherapy with local hepatic perfusion. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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Review

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14 pages, 2170 KiB

Open AccessReview

Effect of Probiotic Fermented Milk Supplementation on Glucose and Lipid Metabolism Parameters and Inflammatory Markers in Patients with Type 2 Diabetes Mellitus: A Meta-Analysis of Randomized Controlled Trials

by Hao Zhong, Lingmiao Wang, Fuhuai Jia, Yongqiu Yan, Feifei Xiong, Khemayanto Hidayat and Yunhong Li

Biology 2024, 13(8), 641; https://doi.org/10.3390/biology13080641 - 21 Aug 2024

Cited by 2 | Viewed by 2176

Abstract

Modulating gut microbiota composition through probiotic administration has been proposed as a novel therapy for type 2 diabetes mellitus (T2DM), and fermented milk is arguably the most common and ideal probiotic carrier. The present meta-analysis was performed to assess the effects of probiotic [...] Read more.

Modulating gut microbiota composition through probiotic administration has been proposed as a novel therapy for type 2 diabetes mellitus (T2DM), and fermented milk is arguably the most common and ideal probiotic carrier. The present meta-analysis was performed to assess the effects of probiotic fermented milk supplementation on glucose and lipid metabolism parameters and inflammatory markers in patients with T2DM using published data from randomized controlled trials (RCTs). The PubMed, Web of Science, and Cochrane Library databases were searched for relevant RCTs. A random-effects model was used to generate the weighted mean difference (WMD) and 95% confidence interval (95% CI). Probiotic fermented milk supplementation reduced the levels of fasting plasma glucose (MD = −17.01, 95% CI −26.43, −7.58 mg/dL; n = 7), hemoglobin A1c (MD = −0.47, 95% CI −0.74, −0.21%; n = 7), total cholesterol (MD = −5.15, 95% CI −9.52, −0.78 mg/dL; n = 7), and C-reactive protein (MD = −0.25, 95% CI −0.43, −0.08; n = 3) but did not significantly affect the levels of HOMA-IR (MD = −0.89, 95% CI −2.55, 0.78; n = 3), triglyceride (MD = −4.69, 95% CI −14.67, 5.30 mg/dL; n = 6), low-density lipoprotein cholesterol (MD = −4.25, 95% CI −8.63, 0.13 mg/dL; n = 7), high-density lipoprotein cholesterol (MD = 1.20, 95% CI −0.96, 3.36 mg/dL; n = 7), and tumor necrosis factor-alpha (MD: −0.58, 95% CI −1.47, 0.32 pg/mL; n = 2). In summary, the present findings provide a crude indication of the potential benefits of probiotic fermented milk supplementation in improving glucose and lipid metabolism and inflammation in patients with T2DM. However, more robust evidence is needed to determine the clinical significance of probiotic fermented milk in the management of T2DM. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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21 pages, 2045 KiB

Open AccessReview

The Therapeutic Potential of the Specific Intestinal Microbiome (SIM) Diet on Metabolic Diseases

by Natural H. S. Chu, Elaine Chow and Juliana C. N. Chan

Biology 2024, 13(7), 498; https://doi.org/10.3390/biology13070498 - 4 Jul 2024

Cited by 2 | Viewed by 1956

Abstract

Exploring the intricate crosstalk between dietary prebiotics and the specific intestinal microbiome (SIM) is intriguing in explaining the mechanisms of current successful dietary interventions, including the Mediterranean diet and high-fiber diet. This knowledge forms a robust basis for developing a new natural food [...] Read more.

Exploring the intricate crosstalk between dietary prebiotics and the specific intestinal microbiome (SIM) is intriguing in explaining the mechanisms of current successful dietary interventions, including the Mediterranean diet and high-fiber diet. This knowledge forms a robust basis for developing a new natural food therapy. The SIM diet can be measured and evaluated to establish a reliable basis for the management of metabolic diseases, such as diabetes, metabolic (dysfunction)-associated fatty liver disease (MAFLD), obesity, and metabolic cardiovascular disease. This review aims to delve into the existing body of research to shed light on the promising developments of possible dietary prebiotics in this field and explore the implications for clinical practice. The exciting part is the crosstalk of diet, microbiota, and gut–organ interactions facilitated by producing short-chain fatty acids, bile acids, and subsequent metabolite production. These metabolic-related microorganisms include Butyricicoccus, Akkermansia, and Phascolarctobacterium. The SIM diet, rather than supplementation, holds the promise of significant health consequences via the prolonged reaction with the gut microbiome. Most importantly, the literature consistently reports no adverse effects, providing a strong foundation for the safety of this dietary therapy. Full article

(This article belongs to the Special Issue Gut Microbiome in Health and Disease (2nd Edition))

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