Bacterial Multidrug Efflux Proteins in Antimicrobial Resistance

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Mechanism and Evolution of Antibiotic Resistance".

Deadline for manuscript submissions: closed (31 January 2021) | Viewed by 3996

Special Issue Editor

School of Biomedical Sciences and Astbury Centre, University of Leeds, Leeds, UK
Interests: membrane proteins; transport proteins; multidrug efflux; antimicrobial resistance; antibiotics; drug discovery and delivery; protein structure and function; chemistry; biochemical and biophysical methods; computational methods
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antimicrobial resistance in bacteria is a global challenge to human and animal health. A major mechanism of antimicrobial resistance is provided by bacterial multidrug efflux proteins. These transport proteins, also known as efflux pumps, actively extrude harmful compounds (e.g., antibiotics) from the bacterial cell membrane and/or cytoplasm. Efflux proteins are present in all bacteria and are highly conserved, indicating ancient origins and essential roles before the antibiotic era, and they contribute to intrinsic, acquired, and phenotypic resistance of bacterial pathogens.

To better understand bacterial multidrug efflux proteins and to discover or develop chemical compounds and strategies to inhibit their function, it is important to study their occurrence and distribution, especially in pathogenic bacteria, and their regulation, structures, ligand interactions, and molecular mechanisms. Seven different families of bacterial multidrug efflux proteins are identified (ABC, RND, MFS, SMR, MATE, PACE, AbgT) with various degrees of characterization [1].

This Special Issue invites articles on the theme of Bacterial Multidrug Efflux Proteins, including methods, strategies, and results on studying their occurrence, distribution and regulation, cloning and amplified expression, purification and reconstitution, activity assays, elucidating structure and molecular mechanism, ligand interactions, and compound screening using various chemical, biochemical, biophysical, and computational approaches.

[1] Ahmad I, Nawaz N, Dermani FK, Kohlan AK, Saidijam M, Patching SG. Bacterial multidrug efflux proteins: A major mechanism of antimicrobial resistance. Curr Drug Targets 2018;19(9):1-13. DOI: 10.2174/1389450119666180426103300.

Prof. Dr. Simon G. Patching
Guest Editor

Manuscript Submission Information

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Keywords

  • antimicrobial resistance
  • efflux pumps
  • molecular mechanism
  • multidrug efflux proteins
  • pathogenic bacteria
  • protein structure and function

Published Papers (1 paper)

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Research

11 pages, 1006 KiB  
Article
The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria
by Ali F. Saleem, Ahreen Allana, Lauren Hale, Alondra Diaz, Raul Salinas, Cristina Salinas, Shahida M. Qureshi, Aneeta Hotwani, Najeeb Rahman, Asia Khan, Anita K. Zaidi, Patrick C. Seed and Mehreen Arshad
Antibiotics 2020, 9(6), 286; https://doi.org/10.3390/antibiotics9060286 - 27 May 2020
Cited by 10 | Viewed by 3552
Abstract
The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum [...] Read more.
The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Stool samples were collected from 104 healthy infants between the ages of 5 and 7 months. Enterobacteriaceae isolates were screened for resistance against several antimicrobial classes. Presence of ESBL and carbapenemase genes was determined using multiplex PCR. Sequence types were assigned to individual strains by multi-locus sequence typing. Phylogenetic analysis of Escherichia coli was done using the triplex PCR method. Forty-three percent of the infants were positive for ESBL-producing Enterobacteriaceae, the majority of which were E. coli. We identified several different ESBL E. coli sequence types most of which belonged to the phylogenetic group B2 (23%) or D (73%). The widespread colonization of infants in a developing country with ESBL-producing Enterobacteriaceae is concerning. The multiple sequence types and reported non-human sources support that multiple non-epidemic MDR lineages are circulating in Pakistan with healthy infants as a common reservoir. Full article
(This article belongs to the Special Issue Bacterial Multidrug Efflux Proteins in Antimicrobial Resistance)
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