Genetic Characterization of Antimicrobial Resistance under the One Health Approach

A special issue of Antibiotics (ISSN 2079-6382). This special issue belongs to the section "Mechanism and Evolution of Antibiotic Resistance".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 462

Special Issue Editors


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Guest Editor
1. Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre S/N, 4169-007 Porto, Portugal
2. BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, Vairão, Portugal
3. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO, Rede de Investigação em Biodi-versidade e Biologia Evolutiva, Laboratório Associado, Universidade do Porto, Vairão, Portugal
4. 1H-TOXRUN—One Health Toxicology Research Unit, University Institute of Health Sciences (IUCS), CESPU, Gandra, Portugal
Interests: antibiotic resistance; bacterial molecular and genomic epidemiology; mobile genetic elements; Enterobacterales; One Health approach; food safety

E-Mail Website
Guest Editor
1. Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
2. CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de 3 Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal
Interests: bacteria; Planctomycetota; antibiotic resistance; novel small molecules; new taxa; One Health
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Special Issue Information

Dear Colleagues,

Microorganisms are vital for maintaining the health of humans, animals, plants, and the environment. However, some can cause infectious diseases, making the existence of effective antimicrobial agents crucial. Antimicrobial resistance (AMR) is a critical global concern exacerbated by the misuse and overuse of antimicrobials in human medicine, agriculture, and veterinary practices. This has led to the emergence and spread of resistant pathogens. AMR genes can be transferred between humans, animals, and the environment, demanding unified efforts and integrated approaches, such as those proposed under the One Health concept. The genetic characterization of AMR involves identifying and analyzing the genetic elements that confer resistance to antimicrobial agents in microorganisms. This process helps us to understand the mechanisms of resistance, track the spread of resistant genes, and develop strategies to combat AMR. Combining genetic characterization with the One Health approach enhances our ability to track and control the spread of resistance. This synergy ensures that interventions are effective across all domains, preserving the efficacy of antimicrobials for future generations. Together, these concepts provide a robust framework for understanding and addressing the complex challenge of AMR, ensuring a coordinated and informed response to this global threat.

Dr. Sandra M. Quinteira
Dr. Olga Maria Lage
Guest Editors

Manuscript Submission Information

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Keywords

  • antimicrobial resistance
  • multidrug-resistant bacteria
  • resistance genes
  • mobile genetic elements
  • molecular mechanisms of resistance
  • One Health
  • epidemiology of AMR
  • AMR surveillance

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Published Papers (1 paper)

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Research

17 pages, 2295 KiB  
Article
The Prevalence of Antimicrobial Resistance Genes in the Environments of Small Ruminant Farms from Central Portugal
by Jaqueline T. Bento, Sara Gomes-Gonçalves, Rita Cruz, Fernando Esteves, Alexandra Lameira Baptista, Maria Aires Pereira, Pedro Caseiro, Pedro Carreira, Luís Figueira, João R. Mesquita, Adriano A. Bordalo and Ana Machado
Antibiotics 2025, 14(6), 576; https://doi.org/10.3390/antibiotics14060576 - 4 Jun 2025
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Abstract
Background: Antimicrobial resistance is a pressing global concern affecting both human and animal health, with environment playing a key role in the dissemination of resistance determinants. This study aimed to investigate the presence of antimicrobial resistance genes (ARGs) associated with tetracyclines, β-lactams, macrolides, [...] Read more.
Background: Antimicrobial resistance is a pressing global concern affecting both human and animal health, with environment playing a key role in the dissemination of resistance determinants. This study aimed to investigate the presence of antimicrobial resistance genes (ARGs) associated with tetracyclines, β-lactams, macrolides, and sulfonamides in environmental matrices collected from 65 sheep and goat farms in central Portugal. Methods: Environmental samples, including water, soil, pasture, and bedding, were analyzed through qPCR for the detection of clinically relevant ARGs. Results: ARGs were detected in 83% of the samples, with over half exhibiting genes from three or more antibiotic classes, suggesting potential multidrug resistance. β-lactamase genes were the most prevalent, followed by those conferring resistance to tetracycline and sulfonamide resistance, while macrolide resistance genes were least frequent. The distribution of ARGs varied by farm type, host species, and municipality. Conclusions: These findings suggest that small ruminant farms serve as important reservoirs for ARGs. The results underscore the need for systematic surveillance and further research into the ecological and genetic factors driving ARG persistence and dissemination in extensive livestock systems, including proper waste management strategies to limit the spread and persistence of antibiotic resistance and mitigate broader public health risks. Full article
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