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11 pages, 300 KiB

Open AccessArticle

Influence of Dietary Phytase Inclusion Rates on Yolk Inositol Concentration, Hatchability, Chick Quality, and Early Growth Performance

by Carlos Alexandre Granghelli, Carrie Louise Walk, Gilson Alexandre Gomes, Tiago Tedeschi dos Santos, Paulo Henrique Pelissari, Brunna Garcia de Souza Leite, Fabricia Arruda Roque, Mário Henrique Scapin Lopes, Lúcio Francelino Araujo and Cristiane Soares da Silva Araujo

Animals 2023, 13(6), 1000; https://doi.org/10.3390/ani13061000 - 9 Mar 2023

Viewed by 2457

Abstract

The aim of this study was to determine the influence of dietary phytase in breeder hens on yolk nutrients, hatchability, chick quality, and growth rate of their progeny, and their subsequent performance to 42 d post-hatch when fed diets with the same phytase [...] Read more.

The aim of this study was to determine the influence of dietary phytase in breeder hens on yolk nutrients, hatchability, chick quality, and growth rate of their progeny, and their subsequent performance to 42 d post-hatch when fed diets with the same phytase concentrations. Breeder hens (n = 216) were divided into 3 groups receiving nutrient-adequate diets with reduced calcium (Ca) and phosphorus (P) (by 0.16% and 0.15%, respectively), supplemented with either 500, 1500, or 4500 FTU/kg phytase from 27 to 50 weeks of age. Eggs were collected at 38 weeks of age and incubated. On the day of hatch, the chick quality and hatchability were determined, and 18 chicks/group were euthanized for yolk sac collection and the determination of inositol and glycerol concentrations. The remaining chicks were divided into three groups, receiving different diets with reduced Ca and P (by 0.16% and 0.15%, respectively), supplemented with 0, 500, or 1500 FTU/kg phytase to 42 d post-hatch. Increasing the phytase concentration in the breeder hen diet linearly (p < 0.05) increased the number of early embryo deaths and decreased the number of late deaths and pips. The inositol concentration in the yolk sac at day of hatch increased (quadratic; p < 0.05) as the phytase dose increased in the breeder hen diet. The breeder hen diet (p < 0.05) influenced the body weight (BW), feed intake (FI), and feed conversion ratio (FCR) up to 21 days of age. The supplementation of breeder hen diets with 1500 FTU/kg phytase increased the concentration of sodium (Na), magnesium (Mg), potassium (K), manganese (Mn), and zinc (Zn) in the yolk sac. The inclusion of phytase doses up to 4500 FTU/kg appeared to influence embryo mortality, chick feed intake, and BW gain to 21 days and the FCR throughout the entire production phase. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

16 pages, 1410 KiB

Open AccessArticle

The Effect of Gum Arabic Supplementation on Growth Performance, Blood Indicators, Immune Response, Cecal Microbiota, and the Duodenal Morphology of Broiler Chickens

by Hani H. Al-Baadani, Rashed A. Alhotan, Abdulaziz A. Al-Abdullatif, Ibrahim A. Alhidary, Abdulrahman S. Alharthi, Saud I. Al-Mufarrej, Maged A. Al-Garadi, Mohammed M. Qaid, Ahmed A. Al-Sagan, Khalid E. Ibrahim and Mahmoud M. Azzam

Animals 2022, 12(20), 2809; https://doi.org/10.3390/ani12202809 - 17 Oct 2022

Cited by 12 | Viewed by 3032

Abstract

Gum Arabic (GA) belongs to the Fabaceae family and contains indigestible soluble fibers (80–85%) that could be fermented by commensal bacteria to enhance performance, immune response, and intestinal integrity. This study aimed to investigate the effects of GA on performance, serum biochemical indicators, [...] Read more.

Gum Arabic (GA) belongs to the Fabaceae family and contains indigestible soluble fibers (80–85%) that could be fermented by commensal bacteria to enhance performance, immune response, and intestinal integrity. This study aimed to investigate the effects of GA on performance, serum biochemical indicators, microbiota, immune-related gene expression, and histological changes in chickens. Six GA levels (0.0, 0.12, 0.25, 0.5, 0.75, 1.0%) were allocated using a total of 432 1-day-old male chickens (12 replicates with 6 chickens each). Growth performance was evaluated on days 10 and 24 of age. Blood parameters, organ pH levels, and intestinal health were determined on day 10 of age. Results showed that GA at 0.12% increased weight gain and 0.12 to 1.0% decreased feed intake but was best in feed conversion ratio and production efficiency except for 1.0% on day 1–10 of age. There was an increase in the thymus weight at GA level 0.25 to 0.75%. GA decreased the pH value of the proventriculus (at 0.50 and 1.0%) as well as the duodenum and cecum (at 0.12 and 1.0%). Chickens fed GA between 0.25 to 1.0% had higher protein and HDL, but lower cholesterol, LDL, and creatinine. Globulin was increased at 0.50% GA, while glucose and triglycerides were decreased (at 0.25 and 0.75% GA, respectively). The immune-related gene expression was reduced, except for 0.25% GA, which increased IL-10. Furthermore, chickens fed GA (0.25 to 0.75%) had higher Lactobacillus spp. and lower Salmonella spp. and Escherichia coli. When chickens received GA, the villus length and length to crypt ratio were higher, which also improved the integrity of intestinal epithelial cells and early duodenal development. We conclude that using GA (0.25 to 0.75%) as a natural prebiotic positively affects the performance, microbiota, immune response, morphology, and gut health of post-hatched chickens. More studies are needed to determine the potential mechanism of GA on broiler chickens. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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10 pages, 270 KiB

Open AccessArticle

Effect of Phytase Level and Form on Broiler Performance, Tibia Characteristics, and Residual Fecal Phytate Phosphorus in Broilers from 1 to 21 Days of Age

by Jose R. Hernandez, Joseph P. Gulizia, John B. Adkins, Martha S. Rueda, Samuel I. Haruna, Wilmer J. Pacheco and Kevin M. Downs

Animals 2022, 12(15), 1952; https://doi.org/10.3390/ani12151952 - 1 Aug 2022

Cited by 6 | Viewed by 3398

Abstract

The present study evaluated the individual and combined effects of coated and uncoated phytase on broiler performance, tibia characteristics, and residual phytate phosphorus (P) in manure. Two repeated studies were conducted using 240-day-old Cobb 500 by-product male broilers per trial. For each trial, [...] Read more.

The present study evaluated the individual and combined effects of coated and uncoated phytase on broiler performance, tibia characteristics, and residual phytate phosphorus (P) in manure. Two repeated studies were conducted using 240-day-old Cobb 500 by-product male broilers per trial. For each trial, birds were assigned to four treatments with four replicate battery cages per treatment (60 birds/trt) and grown for 21 days. Treatments included: (1) negative control (NC), (2) NC + 1000 phytase units (FTU) coated phytase (C), (3) NC + 1000 FTU uncoated phytase (U), and (4) NC + 500 FTU coated + 500 FTU uncoated phytase (CU). Data were analyzed with a one-way ANOVA and means were separated using Tukey’s HSD. In the pooled data for both trials, all treatments with dietary phytase had a higher body weight (BW) and feed consumption (FC) than the NC on day 21 (p < 0.05). Similarly, a six-point reduction was observed for day 1 to 21 feed conversion (FCR) for U and CU (p < 0.05). All treatments with phytase inclusion differed from the NC in every evaluated parameter for bone mineralization (p < 0.05) and had significantly lower fecal phytate P concentrations compared to the NC (p < 0.05). Overall, bird performance was essentially unaffected by phytase form, indicating that combining phytase forms does not appear to offer any advantage to the evaluated parameters from day 1 to 21. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

15 pages, 2517 KiB

Open AccessArticle

Growth Performance, Histological Changes and Functional Tests of Broiler Chickens Fed Diets Supplemented with Tribulus Terrestris Powder

by Maged A. Al-Garadi, Hani H. Al-Baadani and Abdulmohsen H. Alqhtani

Animals 2022, 12(15), 1930; https://doi.org/10.3390/ani12151930 - 28 Jul 2022

Cited by 4 | Viewed by 3377

Abstract

The current experiment aimed to investigate the effects of TT powder on performance parameters and functional tests, as well as on morphological and histological changes in the liver, kidney and ileum in broiler chickens. Commercial broilers (total = 168 females) were used, equally [...] Read more.

The current experiment aimed to investigate the effects of TT powder on performance parameters and functional tests, as well as on morphological and histological changes in the liver, kidney and ileum in broiler chickens. Commercial broilers (total = 168 females) were used, equally divided into three dietary treatments (C = 0.0, T1 = 0.75, and T2 = 1.5 g/kg diet). The growth performance (1–35 days of age), absolute and relative weight, liver and kidney functional tests, intestinal morphology (14 and 35 days of age), and histomorphology of the ileum (35 days of age) were evaluated. At 35 days of age, histopathological changes in the ileum, liver, and kidney were also examined. The results showed that the growth performance and absolute and relative weights of the liver and kidney had no negative effects when dietary supplementation with TT powder was given at 0.75 g/kg diet (T1), whereas a decrease was observed at T2 (p < 0.05). Liver and kidney functional tests showed no significant effects in all feed treatments (14 days), while T1 showed lower (p < 0.05) ALT and AST levels (35 days). T1 exhibited higher weights, lengths, and weight-to-length ratios of the small intestine, and relative lengths of the duodenum (p < 0.05). Histomorphometric measurements of the ileum were higher (p < 0.05) in chickens fed the 0.75 g TT/kg diet, and except for in the goblet cell count and epithelial thickness, there were no differences between treatments (p > 0.05). In T1, hepatocytes were more normal but hepatic sinusoids were dilated, whereas in T2, lymphocytes had infiltrated around the central vein and lining endothelial cells had been lost. The kidney was improved in T1 and T2 compared with the control group. Ileal villi were shorter in T2, and some villi fused with enterocyte necrosis and inflammatory cells accumulated in the lumen. We concluded that TT powder (0.75 g/kg feed) has a safe effect and is healthy for broilers. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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19 pages, 333 KiB

Open AccessArticle

Assessment of Neurodegenerative Changes in Turkeys Fed Diets with Different Proportions of Arginine and Methionine Relative to Lysine

by Magdalena Krauze, Katarzyna Ognik, Dariusz Mikulski and Jan Jankowski

Animals 2022, 12(12), 1535; https://doi.org/10.3390/ani12121535 - 14 Jun 2022

Cited by 5 | Viewed by 1970

Abstract

We postulated that the use of optimal levels and proportions of Arg and Met relative to a low or high concentration of Lys in diets for meat turkeys would reduce the occurrence of metabolic disturbances in the nervous tissue that can lead to [...] Read more.

We postulated that the use of optimal levels and proportions of Arg and Met relative to a low or high concentration of Lys in diets for meat turkeys would reduce the occurrence of metabolic disturbances in the nervous tissue that can lead to neurodegenerative changes. The aim of the study was to determine the effect of various proportions of Lys, Arg, and Met in diets for turkeys, with a low content of Lys in accordance with NRC (Experiment 1) recommendations, and in diets with high Lys levels that are close to the recommendations of breeding companies (Experiment 2) on selected indicators of potential neurodegenerative effects in the brain and liver of turkeys. The Experiment 1 and Experiment 2 was conducted using 864 day-old turkey chicks randomly assigned to six groups, in eight replicates (6 groups × 18 birds × 8 replicates). A full description of the methodology can be found in previously published papers using the same experimental design. Indicators informing about the presence or advancement of neurodegenerative processes in the nervous tissue were determined in the brain and liver (level of: AChE, amyloid-β, GAChE, Tau protein, LRP1, and the degree of DNA methylation). It was established that in the case of both a low (National Research Council, NRC) and a high (British United Turkeys, BUT) level of Lys in the diet of turkeys, the Arg level can be reduced to 90% of the Lys level and Met to 30% of the Lys level, because this does not cause neurodegenerative changes in turkeys. Unfavorable neurodegenerative changes may appear if the Arg level is increased from 100 to 110% of the Lys level recommended by the NRC. However, due to the lack of such a relationship when Arg is increased from 100 to 110% of the Lys level recommended by BUT, at this stage of research no definitive conclusions can be drawn regarding the risk of neurodegenerative changes caused by increasing Arg in the diet of turkeys. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

16 pages, 1074 KiB

Open AccessArticle

The Impact of Curcumin on Growth Performance, Growth-Related Gene Expression, Oxidative Stress, and Immunological Biomarkers in Broiler Chickens at Different Stocking Densities

by Mona H. Hafez, Sara E. El-Kazaz, Badr Alharthi, Heba I. Ghamry, Mohammed A. Alshehri, Samy Sayed, Mustafa Shukry and Yasser S. El-Sayed

Animals 2022, 12(8), 958; https://doi.org/10.3390/ani12080958 - 8 Apr 2022

Cited by 49 | Viewed by 5050

Abstract

Curcumin’s antioxidant properties reduce free radicals and may improve broiler growth. Therefore, the influence of stocking density (SD) and administration of curcumin in the diet on broiler performance was explored to clarify the impact of HSD and curcumin on the performance of growth, [...] Read more.

Curcumin’s antioxidant properties reduce free radicals and may improve broiler growth. Therefore, the influence of stocking density (SD) and administration of curcumin in the diet on broiler performance was explored to clarify the impact of HSD and curcumin on the performance of growth, behavioural patterns, haematological, oxidant/antioxidant parameters, immunity markers, and the growth-related genes expression in broiler chickens. A total of 200 broiler chickens (Cobb 500, 2-weeks old) were allotted into 4 groups; SD (moderate and high) and curcumin (100 and 200 mg/kg diet)-supplemented HSD, respectively. Behavioural observations were performed. After a 28-day experimental period, tissue and blood samples were collected for analysis. Expressions of mRNA for insulin-like growth factor-1 (IGF-1), growth hormone receptor (GHR), myostatin (MSTN), and leptin in liver tissues were examined. HSD birds exhibited lower growth performance measurements, haematological parameters, circulating 3,5,3-triiodothyronine and thyroxine levels, antioxidant activities (GSH-Px, catalase, superoxide dismutase), immunoglobulins (A, G, M), and hepatic GHR and IGF-1 expression values. However, HSD birds even had an increment of serum corticosterone, malondialdehyde, pro-inflammatory cytokine (TNF-a, IL-2, IL-6) levels, hepatic leptin and MSTN expression. Moreover, HSD decreased drinking, feeding, crouching, body care, and increased standing and walking behaviour. The addition of curcumin, particularly at a 200 mg/kg diet, alleviated the effect of HSD through amending growth-related gene expression in the chickens. In conclusion, curcumin can enhance birds’ growth performance, behavioural patterns, and immunity by reducing oxidative stress and up-regulating the growth-related gene expressions of broilers under stressful conditions due to a high stocking density. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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14 pages, 273 KiB

Open AccessArticle

Effect of Ginger Powder on Production Performance, Antioxidant Status, Hematological Parameters, Digestibility, and Plasma Cholesterol Content in Broiler Chickens

by Hanan Al-Khalaifah, Afaf Al-Nasser, Tahani Al-Surrayai, Hanan Sultan, Dalal Al-Attal, Rawan Al-Kandari, Haya Al-Saleem, Aisha Al-Holi and Fatma Dashti

Animals 2022, 12(7), 901; https://doi.org/10.3390/ani12070901 - 31 Mar 2022

Cited by 30 | Viewed by 6657

Abstract

The effect of dietary ginger powder on the production performance, digestibility, hematological parameters, antioxidant status, dietary oxidation stability, and plasma cholesterol content of broiler chickens was investigated. Ginger powder was included in the diet at 0, 5, 10, or 15 g/kg. Total antioxidant [...] Read more.

The effect of dietary ginger powder on the production performance, digestibility, hematological parameters, antioxidant status, dietary oxidation stability, and plasma cholesterol content of broiler chickens was investigated. Ginger powder was included in the diet at 0, 5, 10, or 15 g/kg. Total antioxidant capacity and malondialdehyde in sera samples, superoxide dismutase activity, glutathione peroxidase, catalase, and malondialdehyde in liver samples, and the peroxide value and acid value of the stored diets were evaluated. The results showed that ginger inclusion significantly improved antioxidation indices in broiler sera and liver. Total body weight gain in ginger-supplemented birds was higher than that of control birds (p < 0.048). Supplementing the broiler chickens with ginger powder reduced total feed consumption (p < 0.031). White blood cell counts and the percentage of heterophils in the blood were increased in birds that received ginger supplementation (p < 0.001). The inclusion of ginger in the diet improved dry matter digestibility, crude protein utilization, crude fiber utilization, and ether extract utilization. In addition, blood cholesterol, triglyceride, and very low-density lipoprotein levels were decreased (p < 0.001), and high-density lipoprotein and levels were increased, following the inclusion of ginger in the diet (p < 0.001). Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

17 pages, 291 KiB

Open AccessArticle

Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens

by Hong T. Nguyen, Michael R. Bedford, Shu-Biao Wu and Natalie K. Morgan

Animals 2022, 12(5), 547; https://doi.org/10.3390/ani12050547 - 22 Feb 2022

Cited by 27 | Viewed by 4362

Abstract

This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35. Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated [...] Read more.

This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35. Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0–14) and Grower (d 14–35). Birds fed the low sNSP level presented greater BWG at d 0–14 and lower feed intake at d 14–35 compared to birds fed the medium sNSP level (p < 0.005). At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level. At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p < 0.005). Generally, bird performance and nutrient utilisation was greater in birds fed the corn-based diets compared to the wheat-based diets. These results illustrate that dietary sNSP level and composition influences bird performance and nutrient digestibility. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

11 pages, 274 KiB

Open AccessArticle

Influences of L-Arginine In Ovo Feeding on the Hatchability, Growth Performance, Antioxidant Capacity, and Meat Quality of Slow-Growing Chickens

by Panpan Lu, Thanidtha Morawong, Amonrat Molee and Wittawat Molee

Animals 2022, 12(3), 392; https://doi.org/10.3390/ani12030392 - 7 Feb 2022

Cited by 9 | Viewed by 2843

Abstract

The aim of this study was to evaluate the effects of in ovo feeding (IOF) of L-arginine (Arg) on the hatchability, growth performance, antioxidant capacity, and meat quality of slow-growing chickens. A total of 480 eggs were randomly divided into a non-injected control [...] Read more.

The aim of this study was to evaluate the effects of in ovo feeding (IOF) of L-arginine (Arg) on the hatchability, growth performance, antioxidant capacity, and meat quality of slow-growing chickens. A total of 480 eggs were randomly divided into a non-injected control group (NC group) and a 1% Arg-injected group (Arg group). On day 18 of incubation, 0.5 mL of Arg solution was injected into the embryonic amnion in the Arg group. Upon hatching, 160 mixed-sex chickens were randomly assigned to two groups, with four replicates per group. This experiment lasted for 63 days. The results showed that the hatchability, growth performance, carcass traits, and meat quality were not significantly different (p > 0.05) between the two groups. However, the malondialdehyde (MDA) content was lower (p < 0.05), and the glutathione (GSH) level was higher (p < 0.05) on day of hatching in the Arg group. The total antioxidant capacity (T-AOC) activity was increased (p < 0.05) on day 21 post-hatch in the Arg group compared to that in the NC group. In conclusion, IOF of Arg increased the antioxidant capacity of the breast muscle in the starter period, which may have a positive effect on health status of slow-growing chickens post-hatch. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

17 pages, 473 KiB

Open AccessArticle

Dietary Cinnamon Bark Affects Growth Performance, Carcass Characteristics, and Breast Meat Quality in Broiler Infected with Eimeria tenella Oocysts

by Mohammed M. Qaid, Saud I. Al-Mufarrej, Mahmoud M. Azzam, Maged A. Al-Garadi, Abdulmohsen H. Alqhtani, Abdulaziz A. Al-abdullatif, Elsayed O. Hussein and Gamaleldin M. Suliman

Animals 2022, 12(2), 166; https://doi.org/10.3390/ani12020166 - 11 Jan 2022

Cited by 17 | Viewed by 3084

Abstract

A total of 150 broiler chicks were used to determine the impact of dietary cinnamon bark powder (CBP; Cinnamomum verum) on breast meat quality, growth performance, and carcass characteristics of birds under coccidiosis, as one of the protozoan parasitic diseases. A total [...] Read more.

A total of 150 broiler chicks were used to determine the impact of dietary cinnamon bark powder (CBP; Cinnamomum verum) on breast meat quality, growth performance, and carcass characteristics of birds under coccidiosis, as one of the protozoan parasitic diseases. A total of 5 replicates of birds received 1 of the following 6 groups for 34 days: control groups (1 and 2) received a basal diet without the addition of CBP or salinomycin; group 3 received a basal diet with 0.066 g salinomycin; groups 4–6 were given a basal diet supplemented with 2, 4, and 6 g CBP/kg feed, respectively. On day 21, 4 × 10⁴/100 µL of Eimeria tenella oocysts/bird were challenged, except for the negative control (NC). At the end of the experimental trial, five birds/group were sampled for carcass characteristics and breast attributes. Overall, emeriosis negatively affects slaughter body mass, carcass yield, and the majority of carcass characteristics in birds, and cinnamon can mitigate these effects. Cinnamon groups, particularly at the 2 g level, alleviated the negative effect on performance caused by coccidia infestation to the same or greater extent as the negative control and salinomycin treatment groups. Furthermore, when compared with the other experimental groups, the addition of cinnamon improved some physicochemical properties with some affecting meat quality, such as decreasing MFI and increasing toughness in cinnamon-treated groups. In summary, it can be concluded that CBP can enhance the shelf life, carcass, and quality of birds’ meat by maximizing the productive performance efficiency and breast meat productivity of birds under coccidiosis infestation. Further research is required to investigate the use of cinnamon to optimize the quality of meat and the productivity of both healthy and diseased broilers. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

17 pages, 2882 KiB

Open AccessArticle

Chicken Immune Cell Assay to Model Adaptive Immune Responses In Vitro

by Filip Larsberg, Maximilian Sprechert, Deike Hesse, Gudrun A. Brockmann and Susanne Kreuzer-Redmer

Animals 2021, 11(12), 3600; https://doi.org/10.3390/ani11123600 - 19 Dec 2021

Cited by 4 | Viewed by 4623

Abstract

Knowledge about the modes of action of immunomodulating compounds such as pathogens, drugs, or feed additives, e.g., probiotics, gained through controlled but animal-related in vitro systems using primary cultured peripheral blood mononuclear cells (PBMCs) will allow the development of targeted nutrition strategies. Moreover, [...] Read more.

Knowledge about the modes of action of immunomodulating compounds such as pathogens, drugs, or feed additives, e.g., probiotics, gained through controlled but animal-related in vitro systems using primary cultured peripheral blood mononuclear cells (PBMCs) will allow the development of targeted nutrition strategies. Moreover, it could contribute to the prevention of infectious diseases and the usage of antimicrobials, and further promote the health of the animals. However, to our knowledge, a protocol for the isolation of PBMCs with reduced thrombocyte count from chicken blood and subsequent cell culture over several days to assess the effects of immunomodulating compounds is not available. Therefore, we established an optimized protocol for blood sampling and immune cell isolation, culture, and phenotyping for chicken PBMCs. For blood sampling commercial Na–citrate tubes revealed the highest count of vital cells compared to commercial Li–heparin (p < 0.01) and K3EDTA (p < 0.05) tubes. Using combined dextran and ficoll density gradient separation, the thrombocyte count was significantly reduced (p < 0.01) compared to slow-speed centrifugation with subsequent ficoll. For cell culture, the supplementation of RPMI-1640 medium with 10% chicken serum resulted in the lowest relative cell count of thrombocytes compared to fetal calf serum (FCS) (p < 0.05). To validate the ability of the cell culture system to respond to stimuli, concanavalin A (conA) was used as a positive control. The optimized protocol allows the isolation and cultivation of vital PBMCs with reduced thrombocyte count from chicken blood for subsequent investigation of the modes of action of immunomodulating compounds. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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20 pages, 941 KiB

Open AccessArticle

Influence of Age on the Standardized Ileal Amino Acid Digestibility of Corn and Barley in Broilers

by Mukti Barua, Mohammad Reza Abdollahi, Faegheh Zaefarian, Timothy J. Wester, Channarayapatna Krishnegowda Girish, Peter V. Chrystal and Velmurugu Ravindran

Animals 2021, 11(12), 3575; https://doi.org/10.3390/ani11123575 - 16 Dec 2021

Cited by 12 | Viewed by 2835

Abstract

The aim of this study was to determine the standardized ileal digestibility coefficients (SIDCs) of nitrogen (N) and amino acids (AAs) in corn and barley at six different ages (days 7, 14, 21, 28, 35 and 42) of broilers using the direct method. [...] Read more.

The aim of this study was to determine the standardized ileal digestibility coefficients (SIDCs) of nitrogen (N) and amino acids (AAs) in corn and barley at six different ages (days 7, 14, 21, 28, 35 and 42) of broilers using the direct method. The apparent AA digestibility coefficients were corrected using age-appropriate basal endogenous AA losses. No age effect (p > 0.05) was noted for the SIDC of N in corn. The average SIDC of indispensable AAs (IAAs) and total AAs (TAAs) was influenced in a quadratic manner (p < 0.05) with the values being higher at day 7 that decreased at day 14, increased and plateaued between days 21 and 35 and dropped again at day 42. The average SIDC of dispensable AAs (DAAs) was influenced linearly (p < 0.05). In barley, the SIDC of N and average IAAs, DAAs and TAAs was affected (quadratic; p < 0.001) by age. The digestibility increased from day 7 to 21 and then plateaued up to day 42. The present findings confirm that the SIDC of AA in corn and barley are influenced by broiler age and that the age effect on AA digestibility may need to be considered for precise feed formulation. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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15 pages, 308 KiB

Open AccessArticle

Performance and Egg Quality of Laying Hens Fed Diets Containing Raw, Hydrobarothermally-Treated and Fermented Rapeseed Cake

by Magdalena Kopacz, Aleksandra Alicja Drażbo, Katarzyna Śmiecińska and Katarzyna Ognik

Animals 2021, 11(11), 3083; https://doi.org/10.3390/ani11113083 - 28 Oct 2021

Cited by 8 | Viewed by 3490

Abstract

The present study was conducted to investigate how raw rapeseed cake (RRC), hydrobarothermally-treated rapeseed cake (HRC) and fermented rapeseed cake (FRC) fed to laying hens over a period of 12 weeks affected their performance, and the quality, fatty acid (FA) profile and oxidative [...] Read more.

The present study was conducted to investigate how raw rapeseed cake (RRC), hydrobarothermally-treated rapeseed cake (HRC) and fermented rapeseed cake (FRC) fed to laying hens over a period of 12 weeks affected their performance, and the quality, fatty acid (FA) profile and oxidative stability of eggs. A total of 304 Hy-Line Brown laying hens at 36 weeks of age were distributed in a completely randomized design to four treatment groups with 38 replicates per treatment and two hens per replicate. The birds had ad libitum access to feed and water throughout the study. During the experiment, the birds were fed isonitrogenous and isocaloric diets in mash form, with various protein sources. In the control group (C), soybean meal (SBM) was the main source of dietary protein, whereas the experimental groups were fed diets containing 20% of RRC, HRC or FRC. Hydrobarothermal treatment and fermentation decreased the glucosinolate (GLS) content of RC, and fermentation reduced the concentration of phytate phosphorus (PP). In comparison with the RRC group, layers from the HRC and FRC groups were characterized by higher laying performance, comparable with that in group C. Irrespective of its physical form, RC added to layer diets adversely affected eggshell quality in all experimental groups, whereas albumen quality was highest in the FRC group. In comparison with group C, diets containing RRC, HRC and HRC led to a significant decrease in the content of saturated fatty acids (SFAs), an increase in the proportion of n-3 and n-6 polyunsaturated fatty acids (PUFAs) in the total FA pool in egg yolks, and a decrease in the n-6/n-3 PUFA ratio. The inclusion of RRC, HRC and FRC in layer diets decreased the activity of superoxide dismutase (SOD) in egg yolks, relative to group C. Group FCR eggs were characterized by the highest activity of catalase (CAT) and the lowest lipid peroxides LOOH concentration, compared with the remaining groups. The addition of RC to layer diets did not compromise the sensory quality of eggs, and eggs produced in group FRC received the highest overall score. It can be concluded that the inclusion of 20% RRC, HRC and FRC in layer diets does not compromise the sensory quality of eggs and has a beneficial influence on the FA profile and antioxidant potential of egg yolks. The use of FRC is recommended because it contributes to the highest laying performance, superior albumen quality and the highest sensory quality of eggs, relative to RRC and HRC. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

14 pages, 2484 KiB

Open AccessArticle

Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens

by Yinglu Liu, Jia Feng, Yamin Wang, Jing Lv, Jinghe Li, Lijuan Guo and Yuna Min

Animals 2021, 11(11), 3059; https://doi.org/10.3390/ani11113059 - 26 Oct 2021

Cited by 26 | Viewed by 3411

Abstract

This study aimed to evaluate the effects of fermented corn–soybean meal mixed feed on intestinal barrier function and cecal microbiota in laying hens. A total of 360 Jingfen No.6 laying hens (22 wk-old) were assigned to 4 dietary treatments, which were offered basal [...] Read more.

This study aimed to evaluate the effects of fermented corn–soybean meal mixed feed on intestinal barrier function and cecal microbiota in laying hens. A total of 360 Jingfen No.6 laying hens (22 wk-old) were assigned to 4 dietary treatments, which were offered basal diets (without antibiotics) containing 0, 4, 6 and 8% of fermented mixed feed respectively. The results showed that the pH value and anti-nutritional factor concentrations in fermented mixed feed were lower than those in unfermented feed (p < 0.05). Moreover, fermentation in the feed significantly increased the crude protein content (p < 0.05). Supplementation with fermented feed significantly reduced the crypt depth and increased the villi height:crypt depth ratio of duodenum and jejunum (p < 0.05). Meanwhile, fermented feed increased the secretory immunoglobulin A content and MUC2 mRNA expression of jejunum (p < 0.05). These beneficial effects were exhibited at the addition level ≥6% and microbial composition of caeca in the control, and so 6% fermented feed groups were analyzed. The structure of the gut microbiota was remarkably altered by additions, characterized by increased abundances of some health-promoting bacteria, such as Parasutterella, Butyricicoccus and Erysipelotrichaceae (p < 0.05). In summary, fermented mixed feed modulated cecal flora, subsequently contributing to improvements in intestinal morphology and barrier functions in laying hens. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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18 pages, 3872 KiB

Open AccessArticle

Limitation and Potential Effects of Different Levels of Aging Corn on Performance, Antioxidative Capacity, Intestinal Health, and Microbiota in Broiler Chickens

by Ahsan Mustafa, Shiping Bai, Qiufeng Zeng, Xuemei Ding, Jianping Wang, Yue Xuan, Zhuowei Su and Keying Zhang

Animals 2021, 11(10), 2832; https://doi.org/10.3390/ani11102832 - 28 Sep 2021

Cited by 3 | Viewed by 2191

Abstract

Three-hundred and sixty-day-old male broilers underwent three treatments with six replicates of 20 birds per treatment. The experimental diets included NC: normal corn diet; ACL: lower level (39.6–41.24%) of AC; and ACH: a higher level (56.99–59.12%) of AC. During phase 1 (0–21 d), [...] Read more.

Three-hundred and sixty-day-old male broilers underwent three treatments with six replicates of 20 birds per treatment. The experimental diets included NC: normal corn diet; ACL: lower level (39.6–41.24%) of AC; and ACH: a higher level (56.99–59.12%) of AC. During phase 1 (0–21 d), broilers fed on AC showed lower (p < 0.05) body weight (BW), body weight gain (BWG), and feed conversion ratio (FCR) as compared with the NC group. During phase 2 (22–42 d), the NC group and ACL group showed better (p < 0.05) BW, BWG, and FCR than the ACH group. The footpad lesion score (p = 0.05) and litter moisture percentage (p < 0.05) were found to be higher in the ACH group. During phase 1, the ACL group showed a lower level of malondialdehyde (MDA) contents (p < 0.05) in serum; moreover, catalase (CAT) (p < 0.05) and glutathione peroxidase (GSH-Px) activities (p < 0.05) were found lower in both AC-containing groups. During phase 2, CAT activity in serum was found higher (p < 0.05) in the ACH group. During phase 1, the NC group showed higher CAT (p = 0.05), GSH-Px (p < 0.05), and superoxide dismutase (SOD) activity (p = 0.03); however, it showed lower MDA (p < 0.05) and total-antioxidative capability (T-AOC) (p < 0.05) in the liver. During phase 1, in breast muscle, CAT, SOD, and T-AOC were higher (p < 0.05) in the NC group. During phase 1, total cholesterol and high-density lipoprotein were found to be lower (p < 0.05) in the ACL group. Similarly, triglyceride and low-density lipoprotein were found to be lower (p < 0.05) in the ACL group than the ACH group. During phase 1, villus height was found to be higher (p < 0.05) in the ACH group. Moreover, the goblet cell (GC) was found to be higher (p < 0.05) in the NC group than the ACL group. During phase 2, GC was found to be higher (p < 0.05) in the ACL group. In ileal digesta, during phase 1, acetic acid, propionic acid, and butyric acid (BA) levels were found to be higher (p < 0.05) in the ACL group. In cecal digesta, BA was significantly lower (p < 0.05) in the NC group. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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13 pages, 313 KiB

Open AccessArticle

Soybean Oil Replacement by Poultry Fat in Broiler Diets: Performance, Nutrient Digestibility, Plasma Lipid Profile and Muscle Fatty Acids Content

by Ahmed A. Saleh, Abdulrahman S. Alharthi, Rashed A. Alhotan, Mustafa Shukry Atta and Abdel-Moneim Eid Abdel-Moneim

Animals 2021, 11(9), 2609; https://doi.org/10.3390/ani11092609 - 5 Sep 2021

Cited by 18 | Viewed by 4183

Abstract

Continuous genetic improvements of commercial broiler strains has led to the necessity of using fats in their rations to fulfill a large portion of the energetic requirements. Several fat sources have been introduced in poultry nutrition, such as rendering poultry fat (PF) an [...] Read more.

Continuous genetic improvements of commercial broiler strains has led to the necessity of using fats in their rations to fulfill a large portion of the energetic requirements. Several fat sources have been introduced in poultry nutrition, such as rendering poultry fat (PF) an available and cheap lipid source compared to conventional sources such as soybean oil (SO). The present study investigated the effect of partial or full replacement of SO by PF on performance, nutrient digestibility, blood lipids, and fatty acids (FAs) content of pectoral muscle. Four hundred and eighty one-day-old male Ross-308 chicks were distributed into four experimental groups (12 replicates each): the first group (control) was fed a diet formulated with soybean oil as a fat source while the second to fourth groups (PF25, PF50, and PF100) were fed diets formulated with 25, 50 and 100% of PF as a fat source instead of SO. Results revealed no synergistic effect between SO and PF in any of the studied parameters. Replacing SO by PF did not alter birds’ growth, carcass characteristics, and plasma indices of birds. Abdominal fat% was increased (p < 0.01) in PF50 and PF100. Dry matter digestibility was improved (p < 0.05) in PF50 and PF100, while crude fat and protein digestibility was not affected. Contents of palmitic and docosahexaenoic acids in the pectoral muscle of PF50 and PF100 were reduced (p < 0.01) while concentrations of oleic and linolenic acids, total unsaturated FAs, and polyunsaturated FAs/Saturated FAs ratio were elevated (p < 0.05) in the same groups. Liver thiobarbituric acid reactive substances (TBARS) and muscle vitamin E contents were not altered. The dietary addition of PF greatly improved economic parameters. In conclusion, PF can be used as a lipid source in broiler diets to produce inexpensive meat while maintaining its growth performance. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

17 pages, 875 KiB

Open AccessArticle

Effects of Dietary Supplementation of Salvia officinalis L. in Organic Laying Hens on Egg Quality, Yolk Oxidative Stability and Eggshell Microbiological Counts

by Dimitrios Galamatis, Georgios A. Papadopoulos, Diamanto Lazari, Dimitrios Fletouris, Evanthia Petridou, Georgios I. Arsenos and Paschalis Fortomaris

Animals 2021, 11(9), 2502; https://doi.org/10.3390/ani11092502 - 26 Aug 2021

Cited by 7 | Viewed by 3505

Abstract

Aromatic plants of Labiatae family are used in poultry diets because of their antimicrobial and antioxidant activity. The notion is that hens raised in organic systems face several health and environmental challenges. Hence, the objective here was to assess hens’ performances and the [...] Read more.

Aromatic plants of Labiatae family are used in poultry diets because of their antimicrobial and antioxidant activity. The notion is that hens raised in organic systems face several health and environmental challenges. Hence, the objective here was to assess hens’ performances and the quality of their eggs in such systems following a dietary supplementation of Salvia officinalis L. in powder form. The experiments were conducted over two successive years (1 and 2). They lasted 16 weeks each and involved 198 laying hens aged 40 weeks old randomly assigned to three groups: Con (control diet), Sal-0.5%, and Sal-1.0% (diets supplemented with Salvia officinalis L. at 0.5% or 1.0%, respectively). The malondialdehyde (MDA) levels in egg yolks in year 2 were lower in both Sal-0.5% and Sal-1.0% compared to the Con (p < 0.05). The total number of Enterobacteriaceae in eggshells were lower in Sal-1.0% compared to the Con (p < 0.05) in both years. The results suggest that a dietary supplementation of Salvia officinalis L. at 1.0% improves the antioxidant status and reduces the microbial load of eggs produced in organic systems. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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7 pages, 211 KiB

Open AccessCommunication

Impact of Microbial Protease Enzyme and Dietary Crude Protein Levels on Growth and Nutrients Digestibility in Broilers over 15–28 Days

by Abdul Jabbar, Muhammad Tahir, Ibrahim A. Alhidary, Mutassim A. Abdelrahman, Hani Albadani, Rifat Ullah Khan, Maria Selvaggi, Vito Laudadio and Vincenzo Tufarelli

Animals 2021, 11(9), 2499; https://doi.org/10.3390/ani11092499 - 25 Aug 2021

Cited by 36 | Viewed by 4239

Abstract

In this trial, a 3 × 2 factorial design with different dietary crude protein levels (CP, 17, 19 and 21%) and two levels of exogenous protease (0 and 30,000 IU/kg) was used. A total of 540 two-week old broilers (Ross-308) was randomly allocated [...] Read more.

In this trial, a 3 × 2 factorial design with different dietary crude protein levels (CP, 17, 19 and 21%) and two levels of exogenous protease (0 and 30,000 IU/kg) was used. A total of 540 two-week old broilers (Ross-308) was randomly allocated to experimental diets over 15–28 days of age. The interaction between dietary protein levels and enzyme supplementation showed that body weight gain was significantly (p < 0.05) higher in birds fed CP-19 (1114.7 g) and CP-21 (1108.8 g) with enzymes supplementation. Feed intake was higher (p < 0.05) in broilers fed with CP-17 than CP-19 with supplementation of the protease enzyme. Results also revealed that the feed conversion ratio (FCR) was significantly (p < 0.05) improved in birds fed with CP-19 and CP-21 and protease supplementation. Total tract N retention was lower (p < 0.05) in birds fed CP-17 with no enzyme than the other dietary groups. Similarly, the gross energy (GE) was significantly (p < 0.05) lower in birds fed CP-17 with or without the protease enzyme. Abdominal fat was higher (p < 0.05) in CP-17 (0.96%) without the protease enzyme. It was concluded that a diet at 19% CP with the protease enzyme improved the performance and nutrient digestibility in broilers over 15–28 days. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

15 pages, 5335 KiB

Open AccessArticle

Quercetin Dietary Supplementation Advances Growth Performance, Gut Microbiota, and Intestinal mRNA Expression Genes in Broiler Chickens

by Mervat A. Abdel-Latif, Ahmed R. Elbestawy, Ali H. El-Far, Ahmed E. Noreldin, Mohamed Emam, Roua S. Baty, Ghadeer M. Albadrani, Mohamed M. Abdel-Daim and Hatem S. Abd El-Hamid

Animals 2021, 11(8), 2302; https://doi.org/10.3390/ani11082302 - 4 Aug 2021

Cited by 56 | Viewed by 4906

Abstract

Quercetin was fed to groups of broiler chickens at concentrations of 200, 400, and 800 ppm, and a control group was supplemented with a basal diet. Results revealed that quercetin dietary supplementation numerically improved the growth performance traits and significantly increased (p [...] Read more.

Quercetin was fed to groups of broiler chickens at concentrations of 200, 400, and 800 ppm, and a control group was supplemented with a basal diet. Results revealed that quercetin dietary supplementation numerically improved the growth performance traits and significantly increased (p < 0.05) the European production efficiency factor (EPEF) in the 200 ppm group. The total coliforms and Clostridium perfringens were decreased (p < 0.05) in quercetin-supplemented groups. Conversely, Lactobacillus counts were increased (p < 0.05), due to improvement of the gut microbiota environment in quercetin-supplemented groups. Moreover, the mRNA expression of intestinal Cu/Zn-superoxide dismutase (SOD1), glutathione peroxidase (GSH-Px) and nutritional transporters, including glucose transporter 2 (GLUT2), peptide transporter 1 (PEPT1), and fatty acid synthase (FAS) genes, were significantly upregulated in quercetin-supplemented groups. Quercetin enhanced intestinal morphometry. We can suggest quercetin supplementation in broiler chickens by levels between 200 and 400 ppm to enhance their development and gut environment. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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16 pages, 2708 KiB

Open AccessArticle

Dietary Supplementation of Shredded, Steam-Exploded Pine Particles Decreases Pathogenic Microbes in the Cecum of Acute Heat-Stressed Broilers

by Akshat Goel, Beom-June Kim, Chris-Major Ncho, Chae-Mi Jeong, Vaishali Gupta, Ji-Young Jung, Si-Young Ha, Dong-Hwan Lee, Jae-Kyung Yang and Yang-Ho Choi

Animals 2021, 11(8), 2252; https://doi.org/10.3390/ani11082252 - 30 Jul 2021

Cited by 18 | Viewed by 3413

Abstract

The gut microbiome stimulates nutrient metabolism and could effectively generate heat tolerance in chickens. This study investigates the effects of dietary steam-exploded pine particle (SPP) supplementation and subsequent acute heat stress on productive performance and cecum microbiome in broilers. Eight-day Ross 308 broilers [...] Read more.

The gut microbiome stimulates nutrient metabolism and could effectively generate heat tolerance in chickens. This study investigates the effects of dietary steam-exploded pine particle (SPP) supplementation and subsequent acute heat stress on productive performance and cecum microbiome in broilers. Eight-day Ross 308 broilers were distributed in three groups with 0%, 1%, and 2% SPP in diets. On the 41st day, forty birds were allocated to four groups with ten birds each. The treatments were control diet at thermoneutral temperature (0% NT) and acute heat-stressed (HS) birds fed control (0% HS), 1% (1% HS), and 2% (2% HS) SPP. Parameters recorded were body weight (BW), feed intake (FI), rectal temperature (RT), relative organ weight, and metagenome analysis from cecum samples. Percent difference in BW, FI, and RT was decreased in HS birds. Metagenome analysis revealed similar richness and diversity in microbial communities. The relative abundance of the bacterial genus such as Limosilactobacillus, Drancourtella, and Ihubacter was increased while that of Alistipes, Alkalibacter, Lachnotalea, and Turicibacter was decreased in SPP supplemented HS birds. Concludingly, the production performance of broilers is negatively influenced during HS, and 2% dietary SPP supplementation may reduce the adverse effects of HS by modifying the microbiota in chickens. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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24 pages, 1325 KiB

Open AccessArticle

Impacts of Supplementing Broiler Diets with Biological Curcumin, Zinc Nanoparticles and Bacillus licheniformis on Growth, Carcass Traits, Blood Indices, Meat Quality and Cecal Microbial Load

by Mohamed E. Abd El-Hack, Bothaina A. Alaidaroos, Reem M. Farsi, Diaa E. Abou-Kassem, Mohamed T. El-Saadony, Ahmed M. Saad, Manal E. Shafi, Najah M. Albaqami, Ayman E. Taha and Elwy A. Ashour

Animals 2021, 11(7), 1878; https://doi.org/10.3390/ani11071878 - 24 Jun 2021

Cited by 102 | Viewed by 7202

Abstract

The current study aimed to investigate the influence of dietary zinc nanoparticles (ZnNPs), curcumin nanoparticles (CurNPs), and Bacillus licheniformis (Bl) on the growth, carcass, blood metabolites, and the count of some cecal microorganisms of Indian River (IR) broilers. Chicks were allotted into seven [...] Read more.

The current study aimed to investigate the influence of dietary zinc nanoparticles (ZnNPs), curcumin nanoparticles (CurNPs), and Bacillus licheniformis (Bl) on the growth, carcass, blood metabolites, and the count of some cecal microorganisms of Indian River (IR) broilers. Chicks were allotted into seven experimental groups: control group, 1st, 2nd and 3rd groups were given diets enriched with ZnNPs, CurNPs and Bl (3.0, 5.0 and 2.0 cm³/kg diet, respectively). The 4th, 5th and 6th groups were given diets supplemented with ZnNPs (3.0) + Bl (2.0) (ZP); ZnNPs (3.0) + CurNPs (5.0) (ZC) and ZnNPs (3.0) + CurNPs (5.0) + Bl (2.0) (ZCP) cm³/kg diet, respectively. The results revealed that ZnNPs and CurNPs exhibited a considerable antimicrobial activity against pathogenic bacteria and fungi. They also inhibited the growth of microbes in a range of 50–95 µg/mL. The diet supplemented with ZnNPs, CurNPs, and Bl increased the body weight compared to the control after five weeks of age. Additionally, values of daily feed intake increased in these groups; however, the feed conversion ratio decreased. All values of carcass traits were better than that of the control. The treatments led to decreased abdominal lipids compared to the control. The activity of liver enzymes and malondialdehyde (MDA) activity decreased in the treated groups. In a converse trend, the levels of oxidative enzymes, amylase, protease, lipase and immunoglobulin were higher than that of the control. Meat quality properties were improved and cecal microbial counts were decreased. In conclusion, the ZnNPs, CurNPs, and Bl improved the broiler’s weights, carcass traits, meat quality traits, as well as some blood indices and cecal microbial load. Therefore, the inclusion of ZnNPs, CurNPs, or Bl is recommended for broiler feeding regimens to improve the performance and health status. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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13 pages, 258 KiB

Open AccessArticle

Effect of Different Dietary Betaine Fortifications on Performance, Carcass Traits, Meat Quality, Blood Biochemistry, and Hematology of Broilers Exposed to Various Temperature Patterns

by Ahmed A. Al-Sagan, Abdullah H. Al-Yemni, Alaeldein M. Abudabos, Abdulaziz A. Al-Abdullatif and Elsayed O. Hussein

Animals 2021, 11(6), 1555; https://doi.org/10.3390/ani11061555 - 26 May 2021

Cited by 13 | Viewed by 4301

Abstract

Improving broilers’ production in the hot region is essential to overcome heat-stress challenges. The current experiment examined the effects of betaine’s fortification (0.0, 0.075, 0.10, and 0.15%) to broiler chickens during days 1–40 of age. The growth period was divided into the starter [...] Read more.

Improving broilers’ production in the hot region is essential to overcome heat-stress challenges. The current experiment examined the effects of betaine’s fortification (0.0, 0.075, 0.10, and 0.15%) to broiler chickens during days 1–40 of age. The growth period was divided into the starter (1–18 d) and growing-finishing (19–40 d). During the starter period, there was no heat challenge, and all birds were kept under the same conditions. At 18 days of age, half of the birds were kept under thermos-neutral temperature (TN, 22–24 °C), while the other half were kept under high temperature (HT, 35 °C). However, the production efficiency factor (PEF) was the best (p < 0.05) for birds that received 0.10% betaine. Betaine fortification improved (p < 0.05 and 0.01) body weight gain (BWG), feed conversion ratio (FCR), and production efficiency factor (PEF) in the cumulative finisher heat-stress challenge period (19–40 d). The best performance was achieved at 0.1% betaine fortification with 84 g gain, 4.6 points improvement in FCR, and 24 points improvements in PEF as compared to no betaine fortification. The heat-stressed group consumed less feed (239 g), gained less weight (179 g), converted feed less efficiently (2.6 points), and, as a result, had lower FEF (29 points) as compared to the TN group. Conclusively, heat challenge had a powerful effect on growth performance, meat characteristics, and blood parameters, especially during the grower-finisher period. Betaine fortification (0.1%) during heat stress reduced the negative impact on performance and improved production efficiency, suggesting that betaine is a useful nutritional tool under stress conditions that deserves further investigation. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

10 pages, 6395 KiB

Open AccessArticle

Myostatin and Related Factors Are Involved in Skeletal Muscle Protein Breakdown in Growing Broilers Exposed to Constant Heat Stress

by Xiumei Li, Minhong Zhang, Jinghai Feng and Ying Zhou

Animals 2021, 11(5), 1467; https://doi.org/10.3390/ani11051467 - 20 May 2021

Cited by 8 | Viewed by 2820

Abstract

Heat stress has an adverse effect on the development of poultry farming, which has always aroused great concern. This study was carried out to investigate the protein breakdown mechanism responsible for the suppressive effect of constant heat stress on muscle growth in growing [...] Read more.

Heat stress has an adverse effect on the development of poultry farming, which has always aroused great concern. This study was carried out to investigate the protein breakdown mechanism responsible for the suppressive effect of constant heat stress on muscle growth in growing broilers. A total of 96, 29-day-old, Arbor Acres male broilers were randomly divided into two groups, a thermoneutral control (21 ± 1 °C, TC) and a heat stress (31 ± 1 °C, HS) group, with six replicates in each group and eight birds in each replicate. The trial period lasted for 14 d, and the trial was performed at 60 ± 7% relative humidity, a wind speed of <0.5 m/s and an ammonia level of <5 ppm. The results showed that the average daily feed intake and average daily gain in the HS group were distinctly lower than those in the TC group (p < 0.05), whereas the HS group showed a significantly increased feed conversion ratio, nitrogen excretion per weight gain and nitrogen excretion per feed intake compared to the TC group (p < 0.05). In addition, the HS group showed a significantly reduced breast muscle yield and nitrogen utilization in the broilers (p < 0.05). The HS group showed an increase in the serum corticosterone level (p < 0.05) and a decrease in the thyroxine levels in the broiler chickens (p < 0.05) compared to the TC group, whereas the HS group showed no significant changes in the serum 3,5,3′-triiodothyronine levels compared to the TC group (p > 0.05). Moreover, the HS group showed increased mRNA expression levels of myostatin, Smad3, forkhead box O 4, muscle atrophy F-box and muscle ring-finger 1, but reduced mRNA expression levels of the mammalian target of rapamycin, the protein kinase B and the myogenic determination factor 1 (p < 0.05). In conclusion, the poor growth performance of birds under constant heat stress may be due to an increased protein breakdown via an mRNA expression of myostatin and related factors. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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13 pages, 1010 KiB

Open AccessArticle

Effects of Heat Stress on Gut-Microbial Metabolites, Gastrointestinal Peptides, Glycolipid Metabolism, and Performance of Broilers

by Guangju Wang, Xiumei Li, Ying Zhou, Jinghai Feng and Minhong Zhang

Animals 2021, 11(5), 1286; https://doi.org/10.3390/ani11051286 - 30 Apr 2021

Cited by 39 | Viewed by 4136

Abstract

This paper investigated the effects of heat stress on gut-microbial metabolites, gastrointestinal peptides, glycolipid metabolism, and performance of broilers. Thus, 132 male Arbor Acres broilers, 28-days-old, were randomly distributed to undergo two treatments: thermoneutral control (TC, 21 °C) and high temperature (HT, 31 [...] Read more.

This paper investigated the effects of heat stress on gut-microbial metabolites, gastrointestinal peptides, glycolipid metabolism, and performance of broilers. Thus, 132 male Arbor Acres broilers, 28-days-old, were randomly distributed to undergo two treatments: thermoneutral control (TC, 21 °C) and high temperature (HT, 31 °C). The results showed that the average daily gain (ADG), average daily feed intake (ADFI), and gastric inhibitory polypeptide (GIP) concentration in the jejunum significantly decreased the core temperature, feed conversion ratio (FCR), and ghrelin of the hypothalamus, and cholecystokinin (CCK) in jejunum, and serum significantly increased in the HT group (p < 0.05). Exploration of the structure of cecal microbes was accomplished by sequencing 16S rRNA genes. The sequencing results showed that the proportion of Christensenellaceae and Lachnospiraceae decreased significantly whereas the proportion of Peptococcaceae increased at the family level (p < 0.05). Ruminococcus and Clostridium abundances significantly increased at the genus level. Furthermore, the content of acetate in the HT group significantly increased. Biochemical parameters showed that the blood glucose concentration of the HT group significantly decreased, and the TG (serum triglycerides), TC (total cholesterol), insulin concentration, and the insulin resistance index significantly increased. Nonesterified fatty acid (NEFA) in the HT group decreased significantly. In conclusion, the results of this paper suggest that the poor production performance of broilers under heat stress may be related to short-chain fatty acids (SCFAs) fermented by intestinal microbiota involved in regulating metabolic disorders. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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16 pages, 274 KiB

Open AccessArticle

Research on the Effects of Gender and Feeding Geese Oats and Hybrid Rye on Their Slaughter Traits and Meat Quality

by Dariusz Lisiak, Piotr Janiszewski, Eugenia Grześkowiak, Karol Borzuta, Beata Lisiak, Łukasz Samardakiewicz, Tomasz Schwarz, Krzysztof Powałowski and Krzysztof Andres

Animals 2021, 11(3), 672; https://doi.org/10.3390/ani11030672 - 3 Mar 2021

Cited by 6 | Viewed by 2374

Abstract

The aim of the study was to determine the effect of feeding Zatorska variety geese hybrid rye, oats, or a mixture of both grains (1:1) on slaughter value and meat quality. At 14 weeks old, the birds were separated into three feeding groups [...] Read more.

The aim of the study was to determine the effect of feeding Zatorska variety geese hybrid rye, oats, or a mixture of both grains (1:1) on slaughter value and meat quality. At 14 weeks old, the birds were separated into three feeding groups (n = 12) and were fed between 15 and 17 weeks of age with hybrid rye, oats, or a mixture of these two grains. The research proved the effect of gender and feeding on some slaughter value traits and meat quality of the goslings’ breast meat. It was found that the ganders had a 10% to 15% higher body and carcass weight than the females. No significant differences were observed between the genders within the majority of the physical and chemical characteristics as well as the sensory traits. The feeding type did not have a significant effect on the goslings’ body weight and carcass element share. The birds fed hybrid rye had a 2 percentage points lower slaughter yield than those birds fed oats which was combined with a lower share of subcutaneous fat (measured as the weight of the tissue coming from dissection) in birds fed hybrid rye. The meat of the birds fed hybrid rye had some better physical, chemical characteristics and, in the female goslings, also better sensory quality. The results indicated that hybrid rye may be used in feeding goslings at the end of the growing period, because it did not cause any negative effects on the pre-slaughter body weight and had a positive effect on some meat quality traits, such as better sensory estimation results, higher protein content, and lower drip losses. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

Review

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13 pages, 1957 KiB

Open AccessReview

Protein and Amino Acid Metabolism in Poultry during and after Heat Stress: A Review

by Mohammed M. Qaid and Maged A. Al-Garadi

Animals 2021, 11(4), 1167; https://doi.org/10.3390/ani11041167 - 19 Apr 2021

Cited by 51 | Viewed by 9194

Abstract

This review examined the influence of environmental heat stress, a concern facing modern broiler producers, on protein metabolism and broiler performance, as well as the physiological mechanisms that activate and control or minimize the detrimental impacts of stress. In addition, available scientific papers [...] Read more.

This review examined the influence of environmental heat stress, a concern facing modern broiler producers, on protein metabolism and broiler performance, as well as the physiological mechanisms that activate and control or minimize the detrimental impacts of stress. In addition, available scientific papers that focused on amino acids (AA) digestibility under stress conditions were analyzed. Furthermore, AA supplementation, a good strategy to enhance broiler thermotolerance, amelioration, or stress control, by keeping stress at optimal levels rather than its elimination, plays an important role in the success of poultry breeding. Poultry maintain homeothermy, and their response to heat stress is mainly due to elevated ambient temperature and the failure of effective heat loss, which causes a considerable negative economic impact on the poultry industry worldwide. Reduced feed intake, typically observed during heat stress, was the primary driver for meat production loss. However, accumulating evidence indicates that heat stress influences poultry metabolism and endocrine profiles independently of reduced feed intake. In conclusion, high ambient temperatures significantly reduced dietary AA intake, which in turn reduced protein deposition and growth in broilers. Further studies are required to determine the quantity of the AA needed in warm and hot climates and to introduce genetic tools for animal breeding associated with the heat stress in chickens. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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13 pages, 1240 KiB

Open AccessReview

Date (Phoenix dactylifera L.) by-Products: Chemical Composition, Nutritive Value and Applications in Poultry Nutrition, an Updating Review

by Adel I. Attia, Fayiz M. Reda, Amlan K. Patra, Shaaban S. Elnesr, Youssef A. Attia and Mahmoud Alagawany

Animals 2021, 11(4), 1133; https://doi.org/10.3390/ani11041133 - 15 Apr 2021

Cited by 37 | Viewed by 5084

Abstract

Several by-products, such as whole cull dates, date stones (also called pits, seeds, kernels), sugar-extracted date pulp, are produced from date fruit processing industries. These by-products, particularly date stone meal represent 10 to 20% of the whole dates are wasted, causing environmental issues. [...] Read more.

Several by-products, such as whole cull dates, date stones (also called pits, seeds, kernels), sugar-extracted date pulp, are produced from date fruit processing industries. These by-products, particularly date stone meal represent 10 to 20% of the whole dates are wasted, causing environmental issues. However, the date stone is rich in various nutrients, such as nitrogen-free extract, fiber, fat, and minerals, which could be used as an alternative feed source in poultry nutrition. However, the high fiber content in date stone meal restricts its use in poultry diets. Whole date wastes and date pulp have lower protein, fiber and fat content than those in date stone meal. Several studies have investigated the use of date stone meal and other by-products as a replacement of dietary corn and barley on feed intake, growth, feed efficiency, and nutrient utilization in poultry. The compilation of results obtained from different studies indicates that date fruit by-products, particularly date stone meal, could be incorporated up to 10% levels, replacing corn or barley grains without hampering production performance, and this could reduce the production cost. Moreover, the use of date stone meal at lower levels (5–10%) sometimes shows better growth performance, probably due to the presence of bioactive principles such as antioxidants and phenolic compounds. The use of date stone meal in poultry diets may be practically important under certain circumstances not only under the COVID-19 crisis due to the lockdown of airports, dry ports, ports and traffic restrictions, but also in countries with a limited supply of classical feed resources and which depend on imported ones. Finally, it can recommend that using date stone meal (DSM) up to 5–10% in poultry diets positively affect the productive performance. Due to the variability in the nutritive value of the DSM, developing a formula for metabolizable energy (ME) of DSM = a (intercept) − b (slope) × crude fiber (CF) content should be considered to improve the precision of feed formula for poultry. However, the nutritional value of DSM might not only depend on crude fiber but also on other compounds. Full article

(This article belongs to the Special Issue Recent Advances in Poultry Nutrition and Production)

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