Special Issue "In Vitro Embryo Production in Domestic Animals"

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: 30 November 2021.

Special Issue Editors

Dr. Carolina Maside Mielgo
E-Mail Website
Guest Editor
Postdoctoral Research at SaBio Group (CSIC-UCLM-JCCM), ETSIAM, Albacete, Spain
Interests: oocyte; embryo; fertilization; ovine; bovine; porcine
Dr. Ana Josefa Soler
E-Mail Website
Guest Editor
SaBio IREC (CSIC-UCLM-JCCM), ETSIAM, 02071 Albacete, Spain
Interests: oocyte; embryo; fertilization; ovine; small ruminants
Special Issues and Collections in MDPI journals
Dr. Luis Alberto Vieira
E-Mail Website
Guest Editor
Postdoctoral researcher at Department of Physiology, Faculty of Veterinary, University of Murcia, Murcia, Spain
Interests: oocyte; embryo; fertilization; ovine; bovine; porcine

Special Issue Information

Dear Colleagues,

In this Special Issue, we aim to gather publications regarding in vitro embryo production (IVP) in domestic animals. In recent years, in the IVP system—including in vitro maturation (IVM), fertilization (IVF), and culture (IVC)—embryos produced in vitro have shown significant progress and are being increasingly utilized in domestic animals. IVP is a prized tool, aiding the understanding of early mammalian development in the preservation of gametes from animals of high genetic merit or threatened species, and in the genetic improvement of domestic animals. Furthermore, IVP is an essential instrument for other reproductive biotechnologies, such as cloning, transgenics, or gene editing in a species, serving as a suitable model for human research and xenotransplantation.

We invite researchers to submit original research and review papers on the current status and future outlook of IVP in domestic animals that contribute to the optimization of the procedure. Please contact [email protected] if you are interested.

Dr. Carolina Maside Mielgo
Dr. Ana Josefa Soler
Dr. Luis Alberto Vieira
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • oocyte
  • embryo
  • fertilization
  • ovine
  • bovine
  • porcine
  • equine
  • canine
  • feline

Published Papers (1 paper)

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Research

Article
cAMP Modulators before In Vitro Maturation Decrease DNA Damage and Boost Developmental Potential of Sheep Oocytes
Animals 2021, 11(9), 2512; https://doi.org/10.3390/ani11092512 - 26 Aug 2021
Viewed by 239
Abstract
To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator [...] Read more.
To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator forskolin during a culture period of 2 h before in vitro maturation (pre-IVM) on the nuclear and cytoplasmic maturation features in essential organelles, cumulus cells activity, and in vitro developmental potential of sheep oocytes. Results showed that pre-IVM treatment significantly decreased (p < 0.05) the DNA damage of mature oocytes (pre-IVM = 2.08% ± 3.51% vs. control = 20.58% ± 3.51%) and increased (p ≤ 0.05) expanded blastocyst rates compared to the control (from the total of oocytes: pre-IVM = 23.89% ± 1.47% vs. control = 18.22% ± 1.47%, and from the cleaved embryos: pre-IVM = 45.16% ± 1.73% vs. control = 32.88% ± 1.73%). Considering that oocytes are highly vulnerable to the accumulation of DNA damage because of exposure to in vitro culture conditions, our results suggest that the modulation of intra-oocyte cAMP levels with forskolin and IBMX before IVM might afford oocytes a more effective DNA repair mechanism to overcome damage obstacles and ultimately improve developmental competence. This previously unappreciated action of cAMP modulators could help to develop improved methods for assisted reproduction technologies in animal and clinical research. Full article
(This article belongs to the Special Issue In Vitro Embryo Production in Domestic Animals)
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