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Fermentation, Volume 12, Issue 4

2026 April - 39 articles

Cover Story: Single-cell oil production with oleaginous yeasts traditionally relies on nitrogen limitation, a strategy that restricts the use of nitrogen-rich feedstocks. This reactor-scale study benchmarks four oleaginous yeasts under nitrogen-limited, phosphate-limited, and nutrient-replete conditions in identical 2.5 L stirred-tank reactors. While nitrogen limitation delivered the highest lipid titers, phosphate limitation achieved the highest specific lipid production rate, demonstrating a partial decoupling of lipid synthesis from biomass formation. By systematically comparing growth, lipid accumulation, biomass composition, and fatty acid profiles, the study establishes phosphate limitation as a process-relevant alternative for flexible, sustainable SCO production. View this paper
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Articles (39)

  • Article
  • Open Access
1,151 Views
20 Pages

Effects of Cold Smoking on the Microbiological Characteristics and Volatile Compounds of a Formaella-Type Hard Ewe’s Milk Cheese

  • Thomas Bintsis,
  • Sofia Lalou,
  • Stylianos Exarhopoulos,
  • Ioanna Voulgaridi and
  • Fani Th Mantzouridou

The effect of cold smoking on the physicochemical, microbiological, and aromatic properties of Formaella-type cheese has not been previously investigated. In this study, experimental Formaella-type hard cheeses (≤38% moisture) were produced using...

(This article belongs to the Special Issue Traditional and Innovative Fermented Dairy Products)
  • Article
  • Open Access
1,283 Views
22 Pages

Thraustochytrids are promising alternatives for the production of docosahexaenoic acid (DHA; C22:6 n-3), a long-chain polyunsaturated fatty acid with health benefits. For practical application of this oleaginous microorganism, an efficient cultivatio...

  • Review
  • Open Access
1,472 Views
18 Pages

Streptomycetes in Soil: Community Signals for Biotechnology

  • Marlene Höller,
  • Enes Demiray,
  • Katrin Krause and
  • Erika Kothe

The genus Streptomyces is a major driver of the soil microbial community. These filamentous, exospore-producing bacteria are copious producers of bioactive compounds that are not only used as antibiotics but also affect the soil microbial community i...

(This article belongs to the Section Microbial Metabolism, Physiology & Genetics)
  • Article
  • Open Access
1 Citations
984 Views
18 Pages

Metallothionein (MT) is a multifunctional metal-binding protein with broad applications in medicine, healthcare, and food industries, but its large-scale use is limited by inefficient industrial synthesis. To address this and obtain optimal fermentat...

(This article belongs to the Section Microbial Metabolism, Physiology & Genetics)
  • Article
  • Open Access
2 Citations
1,557 Views
21 Pages

Development and Characterization of a Sunflower-Seed Press-Cake-Based Fermented Food Prototype

  • Sofia Mendo,
  • Beatrice Zignego,
  • Francesca Bonazza,
  • Fabio Masotti,
  • Sara Casati,
  • Sofia Vanerio,
  • Roberto Foschino,
  • Alessio Battistini and
  • Ivano De Noni

Growing environmental and food security concerns have increased interest in circular strategies to valorize agri-food by-products. Sunflower-seed press cake (SSPC), a protein-rich residue from oil extraction, is largely underutilized despite its high...

(This article belongs to the Special Issue Fermentations of Agri-Food By-Products for the Development of Functional Foods)
  • Article
  • Open Access
761 Views
16 Pages

Effects of Yeast Culture Supplementation Rate on Rumen Fermentation and the Rumen Microbial Community in Kazakh Sheep In Vitro

  • Huiying Zhang,
  • Kai Lou,
  • Gulinizier Nueraihemaiti,
  • Yuanyuan Chen,
  • Yan Gao,
  • Jun Zeng,
  • Qing Lin and
  • Xiangdong Huo

To explore the appropriate supplementation rate of yeast culture (YC) in Kazakh sheep during fattening, the effects of different YC supplementation rates on rumen fermentation parameters and microbial community were studied through in vitro rumen flu...

(This article belongs to the Special Issue Ruminal Fermentation: 2nd Edition)
  • Article
  • Open Access
2,381 Views
18 Pages

Grain–Oolong Tea Fermented Beverage Attenuates Adipogenesis-Related Phenotypes in 3T3-L1 Adipocytes

  • Chih-Feng Wang,
  • Chih-Chung Wu,
  • Yi-Jou Chung,
  • Cui-Rou Huang and
  • Ying-Chen Lu

Obesity is a major global health concern, and functional fermented foods have attracted increasing attention for their potential metabolic benefits. Grain–oolong tea fermented beverage (GOFB), produced through a two-step spontaneous fermentatio...

(This article belongs to the Special Issue Advances in Fermented Foods and Beverages)
  • Article
  • Open Access
1 Citations
1,375 Views
13 Pages

Fermentation-Driven Biosynthesis of Natural Carotenoids in Rhodotorula glutinis P4M422: Evaluation of Culture Conditions

  • María de la Luz Herrera-Estrada,
  • José Sandoval-Cortés,
  • Carlos N. Cano-González,
  • Teresinha Gonçalves da Silva,
  • José L. Martínez-Hernández,
  • Miguel A. Aguilar-González,
  • Juan A. Ascacio-Valdés,
  • Mónica L. Chávez-González and
  • Cristóbal N. Aguilar

Carotenoids are natural pigments of high industrial value, with recognized antioxidant properties, and are widely used in the food, cosmetic, and pharmaceutical industries. Oleaginous yeasts, such as Rhodotorula glutinis, represent a promising altern...

(This article belongs to the Special Issue Fermentation-Driven Biotechnological Valorization of Bioactive Compounds from Plants)
  • Article
  • Open Access
1,630 Views
15 Pages

Cloning and Secretory Expression of Aspergillus niger α-amylase with a Novel Synthetic Promoter in Pichia pastoris and Its Application in Apple Juice

  • Fatmanur Mavi,
  • Ayça Uras,
  • Fatma Ersöz,
  • Burcu Emine Tefon-Öztürk,
  • Mehmet İnan,
  • Cüneyt Dinçer,
  • Demet Yıldız-Turgut,
  • Orçun Çınar,
  • Muharrem Gölükcü and
  • Aysun Türkanoğlu-Özçelik
  • + 1 author

Amylase enzyme catalyzes the breakdown of starch by acting on the α-1,4 glycosidic bond. The use of amylases is common in areas such as baking and fruit juice production in the food industry, as well as in the detergent, textile, and paper indu...

(This article belongs to the Special Issue Microbial Metabolism Focusing on Bioactive Molecules)
  • Communication
  • Open Access
693 Views
11 Pages

Exogenous Carbohydrate Effects on Thermoadaptation and Thermostress in Ogataea parapolymorpha Under Different Carbon Sources

  • Denis Atroshenko,
  • Alexandra Roslova,
  • Anastasia Yakobson,
  • Diana Markova,
  • Diana Golovina and
  • Vladimir Tishkov

Thermotolerant methylotrophic yeast Ogataea parapolymorpha is a promising host for high-temperature bioprocesses, yet the effects of carbon source and exogenous carbohydrates on their heat response remain poorly understood. We investigated how growth...

(This article belongs to the Section Yeast)
  • Article
  • Open Access
1,303 Views
15 Pages

Changes in the Bioactive Compounds of Fermented Cabbage Heads Induced by the Addition of Citric and Ascorbic Acid

  • Marina Savić,
  • Milana Vujičić,
  • Lato Pezo,
  • Đorđe Vojnović,
  • Anita Milić,
  • Aleksandra Tepić Horecki and
  • Zdravko Šumić

Cabbage has been cultivated in Serbia for centuries, especially in the Vojvodina Province. Fermentation enhances its functional properties, as fermented cabbage contains live lactic acid bacteria with proven health benefits. Besides improving functio...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
1 Citations
1,989 Views
20 Pages

Valorization of Brewer’s Spent Grains via Aspergillus oryzae Solid-State Fermentation: Production of Lignocellulolytic Enzymes for Biorefinery Applications

  • Anahid Esparza-Vasquez,
  • Sara Saldarriaga-Hernandez,
  • Rosa Leonor González-Díaz,
  • Tomás García-Cayuela and
  • Danay Carrillo-Nieves

Brewer’s spent grain (BSG) is an abundant lignocellulosic by-product whose valorization can support circular bioeconomy strategies. This study evaluated BSG bioconversion by Aspergillus oryzae ATCC 10124 under solid-state fermentation (SSF) to...

(This article belongs to the Special Issue Valorization of Food Waste Using Solid-State Fermentation Technology)
  • Article
  • Open Access
1,133 Views
15 Pages

Robust genetic tools are a prerequisite for causal, perturbation-based tests of redox physiology in acetogens. Here we establish practical genetic entry points for Sporomusa sphaeroides DSM 2875 under strictly anaerobic handling. We first attempted g...

(This article belongs to the Section Industrial Fermentation)
  • Article
  • Open Access
3,099 Views
16 Pages

Green Enzyme Innovation: Improved Laundry Detergent Protease Production Through Solid-State Fermentation

  • José Juan Buenrostro-Figueroa,
  • Sergio Huerta-Ochoa,
  • Cristóbal Noé Aguilar,
  • María Isabel Reyes-Arreozola,
  • Francisco José Fernández and
  • Lilia Arely Prado-Barragán

The increasing demand for environmentally sustainable and efficient laundry detergents has prompted the exploration of innovative biotechnological solutions. This study aims to integrate solid fermentation and by-product valorization for high-quality...

(This article belongs to the Special Issue Fungal Biotechnology: Exploring the Potential of Solid State Fermentation for Sustainable Production)
  • Article
  • Open Access
1 Citations
996 Views
19 Pages

Deterministic Drivers of Microbial Community Succession in Nongxiang Daqu Fermentation: Fungi Exhibit Stronger Environmental Selection Imprints than Bacteria

  • Dongmei Wang,
  • Fei Wang,
  • Ping Tang,
  • Lei Wang,
  • Yusheng Xie,
  • Maosen Xiong,
  • Qian Luo,
  • Yanping Luo,
  • Dan Huang and
  • Lei Yang

Microbial communities are the fundamental determinants of Nongxiang Daqu quality. In this study, we systematically investigated the assembly and succession mechanisms of microbial communities during Nongxiang Daqu fermentation. Our findings reveal th...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
688 Views
17 Pages

Cycloheptylprodigiosin is a promising anticancer candidate that induces cancer cell death accompanied by severe Golgi stress. Although the soybean oil-based optimized MB2216 medium produced a total prodiginine titer approximately three times that of...

(This article belongs to the Section Fermentation Process Design)
  • Article
  • Open Access
4 Citations
1,892 Views
25 Pages

Application of Machine Learning Models (ANN vs. RF) in Optimizing the Fermentation of Sweet-Potato Waste in the Japanese Shochu Industry for Nutritional Enhancement

  • Yukun Zhang,
  • Manabu Ishikawa,
  • Shunsuke Koshio,
  • Saichiro Yokoyama,
  • Na Jiang,
  • Jiayi Chen,
  • Yiwen Tong and
  • Xiaoxiao Zhang

To address the challenge of depleting traditional feed resources, this study aimed to biovalorize sweet potato waste (SPW), a major byproduct of the Japanese shochu industry, into a high-value functional animal feed. An innovative two-stage solid-sta...

(This article belongs to the Special Issue Fermentation Strategies to Enhance Feed Nutritional Value and Optimize Industry Resources)
  • Review
  • Open Access
1,450 Views
17 Pages

Ozone as a Sanitation Method in Winemaking: Improving Fermentation Control in the Context of Climate Change

  • Yaiza Rodríguez,
  • Juan Manuel Del Fresno,
  • Carmen González and
  • Antonio Morata

Climate change presents a challenge for global viticulture due to rising temperatures and water stress, which accelerate grape ripening, increase sugar levels, and reduce acidity. This compromises wine quality and microbial stability, resulting in hi...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
1,224 Views
23 Pages

As probiotic microorganisms must remain viable at a certain level throughout the shelf life of fermented foods, various plant-based prebiotics are added to fermented dairy products. Pea (Pisum sativum L.) is a remarkable food source due to its prebio...

(This article belongs to the Section Probiotic Strains and Fermentation)
  • Article
  • Open Access
1 Citations
1,173 Views
16 Pages

Preliminary Screening of Non-Conventional Yeasts for Olive Mill Wastewater Valorization

  • Gabriella Siesto,
  • Rocchina Pietrafesa,
  • Antonio Caporusso,
  • Giorgia La Rocca,
  • Grazia Alberico,
  • Vito Valerio and
  • Angela Capece

Olive mill wastewater (OMWW) is a highly polluting agro-industrial effluent characterized by elevated organic load, low pH, and high concentrations of phenolic compounds responsible for its phytotoxicity and dark coloration. In this study, 41 non-con...

(This article belongs to the Section Industrial Fermentation)
  • Review
  • Open Access
2,205 Views
19 Pages

Fermentative Dynamics and Emerging Technologies for Their Monitoring and Control in Precision Enology: An Updated Review

  • Jesús Delgado-Luque,
  • Álvaro García-Jiménez,
  • Juan Carbonero-Pacheco and
  • Juan C. Mauricio

Alcoholic fermentation in winemaking is a complex bioprocess governed by physicochemical parameters such as temperature, density, pH, CO2 and redox potential, which critically affect yeast metabolism and wine quality. This review provides an integrat...

(This article belongs to the Special Issue Fermentation Processes: Modeling, Optimization and Control: 3rd Edition)
  • Article
  • Open Access
1,462 Views
13 Pages

Mevalonate is a biochemical precursor to a wide range of isoprenoids. Because the mevalonate pathway uses three moles of acetyl–CoA, native pathways which metabolize acetyl–CoA, including citrate synthase, strongly compete with mevalonate...

(This article belongs to the Section Microbial Metabolism, Physiology & Genetics)
  • Article
  • Open Access
2,443 Views
13 Pages

A Rare Actinomycete from Sicilian Soil: Antimicrobial Potential and Spore Conditioning-Driven Antibiotic Production in Kitasatospora sp. SeTe27

  • Fanny Claire Capri,
  • Enrico Tornatore,
  • Andrea Firrincieli,
  • Gemma Fernánez-García,
  • Rosa Alduina,
  • Angel Manteca and
  • Alessandro Presentato

Actinomycetes are among the richest sources of bioactive secondary metabolites in biotechnology, owing to their remarkable metabolic diversity. Although the genus Streptomyces has been extensively explored and has yielded many clinically important an...

(This article belongs to the Special Issue Novel and Old Insights for Biotechnological Exploitation of Actinomycetota Strain Fermentations)
  • Review
  • Open Access
2 Citations
3,137 Views
44 Pages

Phenyllactic Acid from Lactic Acid Bacteria: A Natural Antimicrobial for Food Biopreservation

  • Emma Mani-López,
  • Beatriz Mejía-Garibay,
  • Ricardo H. Hernández-Figueroa and
  • Aurelio López-Malo

Phenyllactic acid (PLA), a natural antimicrobial metabolite produced by lactic acid bacteria (LAB), has emerged as a key compound for biopreservation in food systems. The aims of this review are to summarize the main findings on LAB-producing strains...

(This article belongs to the Special Issue The Roles of Lactic Acid Bacteria in Food Fermentation)
  • Article
  • Open Access
1 Citations
2,509 Views
21 Pages

The Formation of Aroma Compounds During Fermentation in Relation to Yeast Nutrient Source in Sauvignon Blanc Wine

  • Zorica Lelova Temelkova,
  • Helena Baša Česnik,
  • Andreja Vanzo and
  • Klemen Lisjak

This study aimed to determine the effects of diammonium phosphate (DAP) and yeast autolysates (organic nutrients) added during alcoholic fermentation on the content and profile of aroma compounds in Sauvignon Blanc wines. Sequential additions of eith...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Review
  • Open Access
3 Citations
3,092 Views
34 Pages

Recent Advances in Natural Product Biosynthesis and Yield Improvement Strategies Using Yarrowia lipolytica

  • Zhaorui Gu,
  • Xiaojing Li,
  • Freddie Moore,
  • Anil Kumar Jamithireddy,
  • Steven Bates and
  • Nicholas J. Harmer

Microorganisms are increasingly being used for the industrial production of raw materials for food, chemical products and pharmaceuticals. The unconventional yeast Yarrowia lipolytica has a rising profile as a platform for industrial biotechnology. I...

(This article belongs to the Special Issue Yarrowia lipolytica: A Beneficial Yeast as a Biofactory for Biotechnological Applications: 3rd Edition)
  • Article
  • Open Access
1,548 Views
12 Pages

Effect of Variations in the Gas Outlet Location on an In Vitro Rumen Simulation Technique (RUSITEC®) System

  • Luiza N. C. Silva,
  • Isabela F. Carrari,
  • Ícaro R. R. Castro,
  • Giulia B. C. Leite,
  • Amanda M. Cezar,
  • Eduardo M. Paula and
  • Marcos I. Marcondes

The rumen simulation technique (RUSITEC®) is a known model for research in rumen microbiology and fermentation. However, our research group observed inconsistencies in gas production across trials. This study investigated the effects of different...

(This article belongs to the Special Issue 10th Anniversary of Fermentation: Feature Papers in the “Fermentation Process Design” Section)
  • Article
  • Open Access
1 Citations
1,769 Views
18 Pages

In this study, kefir production was investigated using both commercial kefir cultures and kefir grains, with milk treated at different UV-C doses and flow rates. The flow rate was set to 25 or 50 mL/min, and doses of 43.2 and 21.6 J/mL were applied a...

(This article belongs to the Special Issue Recent Advances in Microbial Fermentation in Foods and Beverages, 2nd Edition)
  • Article
  • Open Access
1 Citations
1,501 Views
13 Pages

Effects of Inoculating Lignin-Degrading Bacteria Isolated from Ruminant Rectum on In Vitro Rumen Diet Degradation

  • Chatchai Kaewpila,
  • Pongsatorn Gunun,
  • Nikom Srikacha,
  • Chanon Suntara and
  • Waroon Khota

In ruminant nutrition, the lignocellulosic complex is a primary constraint limiting the utilization of dietary fiber. The objective of this study was to evaluate the effects of inoculating lignin-degrading bacteria (LDB) isolated from the ruminant re...

(This article belongs to the Special Issue Fermentation Technology for Animals in Thailand: From Feed to Metabolites)
  • Article
  • Open Access
1,195 Views
16 Pages

The effects of substituting pelleted diets manufactured from cassava, chaya, and mulberry leaves for concentrate on growth performance, feed intake, rumen fermentation, and microbial protein synthesis in beef cattle were evaluated. Four beef cattle (...

(This article belongs to the Special Issue Fermentation Technology for Animals in Thailand: From Feed to Metabolites)
  • Article
  • Open Access
1 Citations
1,577 Views
15 Pages

Dynamic Regulation Engineering of Plasmid Copy Number Based on CRISPRi in Saccharomyces cerevisiae

  • Ying Xu,
  • Tingting Xu,
  • Tao Jiang,
  • Xiaoyi Wang,
  • Peipei Zhao,
  • Kuidong Xu,
  • Xuekui Xia and
  • Lixin Zhang

Plasmid copy number (PCN) is a key factor limiting the expression level of heterologous proteins in yeast. Static strategies for enhancing PCN, such as reducing the transcriptional intensity of selection markers or increasing selection pressure, only...

(This article belongs to the Special Issue Yeasts as Microbial Cell Factories: Diversity, Biotechnology Potential and Applications)
  • Review
  • Open Access
1,644 Views
18 Pages

Expanding the Application of Threonine: Industrial Biomanufacturing of Threonine and Its Derivatives

  • Liwen Lu,
  • Lin Su,
  • Qingjing Huang,
  • Xiao Zou,
  • Bangmeng Zhou,
  • Jun Kang,
  • Yang Li,
  • Jiamin Zhang and
  • Jie Cheng

Currently, the production methods for L-threonine (L-Thr) mainly include chemical synthesis, protein hydrolysis, and microbial fermentation. Among these, microbial fermentation has become an important method for the industrial production of L-Thr, ow...

(This article belongs to the Special Issue Microbial Fermentation Technology: A Biological Engine Driving Innovation in the Food Industry)
  • Article
  • Open Access
1,140 Views
11 Pages

The present study was conducted to demonstrate the effects of Codium fragile extract on methane production using in vitro and in vivo experiments. An in vitro batch experiment was conducted to evaluate different inclusion levels of Codium fragile ext...

(This article belongs to the Special Issue Research Progress of Rumen Fermentation, 2nd Edition)
  • Article
  • Open Access
1,534 Views
14 Pages

Modelling of Cordycepin Production by an Engineered Aspergillus oryzae Under Different Substrates

  • Siwaporn Wannawilai,
  • Jutamas Anantayanon,
  • Thanaporn Dechpreechakul,
  • Kobkul Laoteng and
  • Sukanya Jeennor

Given the therapeutic potential of bioactive cordycepin in medical and healthcare products, precision fermentation using an engineered strain of Aspergillus oryzae was performed to enhance cordycepin production. To understand and predict the dynamics...

(This article belongs to the Special Issue Fungi for Bioprocesses: Food, Biofuel, Biocompounds and Wastewater Treatment)
  • Review
  • Open Access
7 Citations
4,078 Views
42 Pages

Functional Foods from Edible Mushrooms and Mycelia: Processing Technologies, Health Benefits, Innovations, and Market Trends

  • Lorena Vieira Bentolila de Aguiar,
  • Larissa Batista do Nascimento Soares,
  • Giovanna Lima-Silva,
  • Daiane Barão Pereira,
  • Vítor Alves Pessoa,
  • Aldenora dos Santos Vasconcelos,
  • Roberta Pozzan,
  • Josilene Lima Serra,
  • Ceci Sales-Campos and
  • Walter José Martínez-Burgos
  • + 1 author

The global functional food market continues to expand, and edible mushrooms are emerging as high-value ingredients due to their rich nutritional profile, particularly their high protein content, balanced amino acid composition, and dietary fiber. Thi...

(This article belongs to the Special Issue Fermented Foods for Boosting Health: 2nd Edition)
  • Article
  • Open Access
1 Citations
1,504 Views
21 Pages

Phosphate Versus Nitrogen Limitation: A Reactor-Scale Process Comparison for Single-Cell Oil Production in Oleaginous Yeasts

  • Kevin Edward Schulz,
  • Paula Hegmann,
  • Bastian Dreher,
  • Lena Regenauer,
  • Carlota Delso Muniesa,
  • Wolfgang Frey,
  • Katrin Ochsenreither and
  • Anke Neumann

Industrial production of single-cell oils (SCOs) by oleaginous yeasts relies predominantly on nitrogen limitation, which constrains process flexibility when nitrogen-rich substrates are used. Although phosphate limitation has been reported as an alte...

(This article belongs to the Section Yeast)
  • Article
  • Open Access
2,018 Views
20 Pages

A Day in the Life of a Sourdough Leaven from Feeding to Maturity

  • Louis Levinger,
  • Monisha Sherpa,
  • Julia Gelman,
  • Mariapia Dibonaventura and
  • Rabindra Mandal

Fermentation is a type of biological process conducted domestically or commercially to preserve foods and beverages, produce alcohol, add nutritional value and improve aroma and flavor. The natural fermentation of flour in water to obtain a leaven fo...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
1 Citations
1,728 Views
17 Pages

This study investigated the impacts of diverse aeration processes (continuous aeration vs. intermittent aeration) and aeration rates on the aerobic composting process. The key properties examined include temperature, oxygen dynamics, lignocellulose d...

(This article belongs to the Section Fermentation Process Design)
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Fermentation - ISSN 2311-5637