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Fermentation, Volume 11, Issue 10

October 2025 - 55 articles

Cover Story: α-Linolenic acid (ALA) is an essential omega-3 fatty acid with health benefits and applications in food and feed. Plant oils are the main source, but their limited supply challenges sustainability. We developed ALA production via microbial fermentation using Aspergillus oryzae, a Generally Recognized As Safe organism. By identifying its endogenous ω-3 desaturase gene (fad3), essential for ALA biosynthesis, and overexpressing fad3 through self-cloning, we obtained the Aofad3-MC strain, which achieved an ALA content of 30.7% of total lipids. Whole-genome and k-mer analyses confirmed the absence of vector-derived sequences. This strategy enables sustainable and socially acceptable ALA production and provides a platform for functional lipid biosynthesis in food, feed, and industrial applications. View this paper
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Articles (55)

  • Review
  • Open Access
1,062 Views
17 Pages

Recent Advances in Biosurfactant Production in Solid-State Fermentation

  • Ma. Guadalupe Bustos-Vázquez,
  • Luis V. Rodríguez-Durán,
  • María Alejandra Pichardo-Sánchez,
  • Nubia R. Rodríguez-Durán,
  • Nadia A. Rodríguez-Durán,
  • Daniel Trujillo-Ramírez and
  • Rodolfo Torres-de los Santos

Biosurfactants are amphiphilic molecules synthesized by some microorganisms. Biosurfactants have a wide range of applications in fields such as the bioremediation, petroleum, and pharmaceutical industries. Currently, biosurfactant production is carri...

  • Article
  • Open Access
777 Views
15 Pages

Effect of Lignite Addition on Maturity and Bacterial Community Assembly in Co-Composting of Goat Manure and Corn Straw

  • Lalete Yi,
  • Bo Wang,
  • Chula Sa,
  • Chunhua Zhang,
  • Wenting Li,
  • Yuquan Wei and
  • Haizhou Sun

This study investigated the effect of lignite addition on maturity acceleration and bacterial community assembly mechanisms through high-throughput sequencing and iCAMP null model analysis in Co-composting of goat manure and corn straw. Three treatme...

  • Article
  • Open Access
852 Views
15 Pages

Incrocio Bruni 54 is a little-known white grape variety developed in the Marche region (Italy) from a cross between Verdicchio and Sauvignon Blanc to combine aromatic freshness with structure. In light of the growing interest in minor and autochthono...

  • Systematic Review
  • Open Access
1,437 Views
29 Pages

From Tea Fermentation to New Technologies: Multisectoral Applications of Kombucha SCOBY Through the Lens of Methodi Ordinatio

  • Nicole de M. Vianna,
  • Gabriel Albagli,
  • Adejanildo da S. Pereira and
  • Priscilla F. F. Amaral

The Symbiotic Culture of Bacteria and Yeast (SCOBY) is a cellulose-based biofilm resulting from the fermentation of sweetened tea by a microbial consortium of acetic acid bacteria and yeasts. This study applies the Methodi Ordinatio technique to syst...

  • Review
  • Open Access
1,303 Views
23 Pages

β-Glucosidase: Progress from Basic Mechanism to Frontier Application

  • Linqing Li,
  • Hanyu Liu,
  • Tianyi Liu,
  • Jingyi Mi,
  • Ruitao Cai and
  • Huilian Xu

β-glucosidase is a kind of enzyme that can hydrolyze β-glucosidase bonds, and it plays a key role in many fields, such as lignocellulose degradation and wine brewing. The global β-glucosidase market is currently estimated to be USD 40...

  • Article
  • Open Access
1,005 Views
21 Pages

Saline–alkaline soils are becoming prevalent across the globe, decreasing the availability of forage for animals and threatening sustainable animal production. This study evaluated the effects of a NaCl-supplemented alfalfa-based total mixed ra...

  • Article
  • Open Access
795 Views
22 Pages

This study investigated the fermentation of beef burgers enriched with varying quantities (5% and 10%) of hazelnut skin powder using Lactobacillus acidophilus and Lactiplantibacillus plantarum cultures. The physicochemical, textural, microbiological,...

  • Article
  • Open Access
1,050 Views
20 Pages

α-Linolenic Acid Production in Aspergillus oryzae via the Overexpression of an Endogenous Omega-3 Desaturase Gene

  • Hiroki Kikuta,
  • Hirotoshi Sushida,
  • Tsuyoshi Tanaka,
  • Eiichi Kotake,
  • Wakako Tsuzuki,
  • Ryota Hattori,
  • Satoshi Suzuki,
  • Ken-Ichi Kusumoto and
  • Junichi Mano

α-Linolenic acid (ALA) is an important essential omega-3 (ω-3) polyunsaturated fatty acid for the maintenance of human health. Although ALA has traditionally been obtained from plant sources, microbial fermentation has emerged as a promis...

  • Article
  • Open Access
973 Views
17 Pages

Fermentation Strategies to Improve Argentinian Kefir Quality: Impact of Double Fermentation on Physicochemical, Microbial, and Functional Properties

  • Raúl Ricardo Gamba,
  • Andrea Ibáñez,
  • Sofía Sampaolesi,
  • Pablo Mobili and
  • Marina Alejandra Golowczyc

This present study investigated the microbial dynamics, physicochemical and functional properties, and sensory characteristics of kefir produced by two different approaches: traditional kefir obtained directly from grains and kefir manufactured throu...

  • Editorial
  • Open Access
911 Views
3 Pages

The interest in food microbial fermentation has progressively increased in recent decades. This Special Issue collected original research and review articles dealing with the use of microbial cultures aimed at improving the organoleptic and nutrition...

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Fermentation - ISSN 2311-5637