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Separations, Volume 12, Issue 6

June 2025 - 33 articles

Cover Story: The development of chromatographic processes to purify biopharmaceuticals requires tremendous experimental effort. The state-of-the-art approach, Design of Experiments, enables a systematic procedure limited to a design space described by a statistical, interpolating model; however, the experimental burden remains high. Process development would benefit from extrapolating models to reduce this burden and enable deeper process understanding and knowledge transfer. Nevertheless, such models currently lack predictive power. In an academic–industrial collaboration, we present a new biophysics-based adsorption model for developing ion-exchange chromatography processes for pharmaceuticals at a preparative scale. This model achieves high predictive power across low and high antibody load densities and even captures charge variant behavior. View this paper
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Articles (33)

  • Article
  • Open Access
1,009 Views
23 Pages

The development of ion exchange chromatography to polish biopharmaceuticals requires extensive experimental benchmarking. As part of the Design of Experiments (DoE), statistical models increased efficiency somewhat and are still state of the art; how...

  • Article
  • Open Access
1,120 Views
18 Pages

Insights into the Adsorptive Separation of Ethylene/Ethane in LTA-Type Zeolites

  • Xiaohui Zhao,
  • Shixue Zhou,
  • Magdy Abdelghany Elsayed,
  • Zhongyuan Chen,
  • Chunhui Jiang,
  • Yongli Hu and
  • Gumawa Windu Manggada

Understanding the competitive adsorption mechanism is essential for the development of adsorptive separation of ethylene (C2H4) and ethane (C2H6). In this work, density functional theory calculations and molecular dynamics simulations were employed t...

  • Article
  • Open Access
1,008 Views
15 Pages

Essential oils (EOs), which are complex mixtures of plant-derived volatile compounds, have been utilized for centuries in the medical, food, and pharmaceutical industries because of their diverse biological properties. In recent years, there has been...

  • Review
  • Open Access
2,312 Views
16 Pages

Volatile C2–C5 olefins are important bulk chemicals in the polymer industry. Traditionally, C2–C5 olefins are produced from cracked petroleum resources using an energy-consuming and hazardous distillation method. Currently, volatile olefi...

  • Article
  • Open Access
713 Views
11 Pages

Analysis on the Main Components of Selenium-Enriched Premna microphylla Leaves and Processed Tofu

  • Jianan Wang,
  • Chunli Chen,
  • Fangjie Mou,
  • Bin Wang and
  • Jingzhou Dong

Premna microphylla is a medicinal plant species distributed in Southeast Asia and China. P. microphylla leaves have been widely used for processing edible gels called Chai tofu, which have many medicinal values, such as clearing heat and detoxifying....

  • Article
  • Open Access
895 Views
13 Pages

This study aims to develop a simultaneous analytical method for detecting 19 pesticides, including 4,4′-DDD, in fishery products using gas chromatography with micro-electron capture detection (GC-μECD) and gas chromatography–tandem mas...

  • Article
  • Open Access
957 Views
14 Pages

Herein, a simple and effective analytical method was developed to monitor etoricoxib concentrations in human urine samples. Etoricoxib is a nonsteroidal anti-inflammatory drug for pain and inflammation relief in conditions such as osteoarthritis and...

  • Article
  • Open Access
2 Citations
1,025 Views
15 Pages

Multi-Template Molecularly Imprinted Polymers Coupled with a Solid-Phase Extraction System in the Selective Determination of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Environmental Water Samples

  • David Aurelio-Soria,
  • Giaan A. Alvarez-Romero,
  • Maria E. Paez-Hernandez,
  • I. Perez-Silva,
  • Miriam Franco-Guzman,
  • Gabriela Islas and
  • Israel S. Ibarra

A simple, fast, and low-cost pre-concentration methodology based on the application of multi-template molecularly imprinted polymers (mt-MIP) in a solid-phase extraction system coupled with capillary electrophoresis was developed for the determinatio...

  • Article
  • Open Access
894 Views
18 Pages

An Optimized Pathway for Nitrate Removal from Aqueous Solution by Environmentally Friendly Calabash Gourd Shell Adsorbent Based on Experimental Design Methodology

  • Goran S. Nikolić,
  • Nataša Simonović,
  • Miloš Durmišević,
  • Nada Nikolić,
  • Dragana Marković Nikolić,
  • Milena Nikolić,
  • Grozdanka Bogdanović and
  • Aleksandar Bojić

The aim of this research is to optimize the process parameters for nitrate adsorption from aqueous solutions using ammonium modified calabash gourd shell (CGS). Two types of experimental design (DoE) methodology were implemented in the optimization....

  • Article
  • Open Access
1 Citations
869 Views
16 Pages

The rapid isolation of target constituents from natural products poses a significant challenge and is a key focus in current research. The Hosta plantaginea flower (HPF), a traditional Chinese medicinal herb, is primarily used to treat inflammatory d...

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Separations - ISSN 2297-8739