Next Article in Journal
Impact of Pre-Blood Collection Factors on Plasma Metabolomic Profiles
Previous Article in Journal
Alterations in Tissue Metabolite Profiles with Amifostine-Prophylaxed Mice Exposed to Gamma Radiation
Article

An NMR-Based Approach to Identify Urinary Metabolites Associated with Acute Physical Exercise and Cardiorespiratory Fitness in Healthy Humans—Results of the KarMeN Study

1
Institute of Sports and Sports Science, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany
2
Department of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, 76131 Karlsruhe, Germany
*
Author to whom correspondence should be addressed.
Present address: Institute of Movement and Sport, Karlsruhe University of Education, 76133 Karlsruhe, Germany.
Present address: TSG 1899 Hoffenheim, 74939 Zuzenhausen, Germany.
Metabolites 2020, 10(5), 212; https://doi.org/10.3390/metabo10050212
Received: 16 April 2020 / Revised: 15 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
Knowledge on metabolites distinguishing the metabolic response to acute physical exercise between fit and less fit individuals could clarify mechanisms and metabolic pathways contributing to the beneficial adaptations to exercise. By analyzing data from the cross-sectional KarMeN (Karlsruhe Metabolomics and Nutrition) study, we characterized the acute effects of a standardized exercise tolerance test on urinary metabolites of 255 healthy women and men. In a second step, we aimed to detect a urinary metabolite pattern associated with the cardiorespiratory fitness (CRF), which was determined by measuring the peak oxygen uptake (VO2peak) during incremental exercise. Spot urine samples were collected pre- and post-exercise and 47 urinary metabolites were identified by nuclear magnetic resonance (NMR) spectroscopy. While the univariate analysis of pre-to-post-exercise differences revealed significant alterations in 37 urinary metabolites, principal component analysis (PCA) did not show a clear separation of the pre- and post-exercise urine samples. Moreover, both bivariate correlation and multiple linear regression analyses revealed only weak relationships between the VO2peak and single urinary metabolites or urinary metabolic pattern, when adjusting for covariates like age, sex, menopausal status, and lean body mass (LBM). Taken as a whole, our results show that several urinary metabolites (e.g., lactate, pyruvate, alanine, and acetate) reflect acute exercise-induced alterations in the human metabolism. However, as neither pre- and post-exercise levels nor the fold changes of urinary metabolites substantially accounted for the variation of the covariate-adjusted VO2peak, our results furthermore indicate that the urinary metabolites identified in this study do not allow to draw conclusions on the individual’s physical fitness status. Studies investigating the relationship between the human metabolome and functional variables like the CRF should adjust for confounders like age, sex, menopausal status, and LBM. View Full-Text
Keywords: metabolomics; urinary metabolome; urinary metabolites; NMR spectroscopy; acute physical exercise; cardiorespiratory fitness; VO2peak; exercise metabolomics metabolomics; urinary metabolome; urinary metabolites; NMR spectroscopy; acute physical exercise; cardiorespiratory fitness; VO2peak; exercise metabolomics
Show Figures

Figure 1

MDPI and ACS Style

Kistner, S.; Rist, M.J.; Döring, M.; Dörr, C.; Neumann, R.; Härtel, S.; Bub, A. An NMR-Based Approach to Identify Urinary Metabolites Associated with Acute Physical Exercise and Cardiorespiratory Fitness in Healthy Humans—Results of the KarMeN Study. Metabolites 2020, 10, 212. https://doi.org/10.3390/metabo10050212

AMA Style

Kistner S, Rist MJ, Döring M, Dörr C, Neumann R, Härtel S, Bub A. An NMR-Based Approach to Identify Urinary Metabolites Associated with Acute Physical Exercise and Cardiorespiratory Fitness in Healthy Humans—Results of the KarMeN Study. Metabolites. 2020; 10(5):212. https://doi.org/10.3390/metabo10050212

Chicago/Turabian Style

Kistner, Sina, Manuela J. Rist, Maik Döring, Claudia Dörr, Rainer Neumann, Sascha Härtel, and Achim Bub. 2020. "An NMR-Based Approach to Identify Urinary Metabolites Associated with Acute Physical Exercise and Cardiorespiratory Fitness in Healthy Humans—Results of the KarMeN Study" Metabolites 10, no. 5: 212. https://doi.org/10.3390/metabo10050212

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop