Microorganisms, Volume 8, Issue 11 (November 2020) – 217 articles
Cover Story (view full-size image): Galleria mellonella larvae possess intrinsic autofluorescence that limits the use of fluorescently tagged bacteria for infection studies. To bypass this problem, a promoter probe vector expressing bioluminescence was constructed. Additionally, an RNA extraction protocol was optimized to study transcriptional gene levels during an in vivo infection. As proof of concept, the role of the different ribonucleotide reductase genes during a P. aeruginosa infection was studied. By using both methodologies, variable gene expression levels were successfully measured in the larvae at different time points. Both approaches enable a better study and monitoring of in vivo bacterial infections. View this paper
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