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Microorganisms, Volume 8, Issue 11

2020 November - 217 articles

Cover Story: Galleria mellonella larvae possess intrinsic autofluorescence that limits the use of fluorescently tagged bacteria for infection studies. To bypass this problem, a promoter probe vector expressing bioluminescence was constructed. Additionally, an RNA extraction protocol was optimized to study transcriptional gene levels during an in vivo infection. As proof of concept, the role of the different ribonucleotide reductase genes during a P. aeruginosa infection was studied. By using both methodologies, variable gene expression levels were successfully measured in the larvae at different time points. Both approaches enable a better study and monitoring of in vivo bacterial infections. View this paper
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Articles (217)

  • Article
  • Open Access
23 Citations
3,542 Views
10 Pages

Detection of the Phenicol–Oxazolidinone Resistance Gene poxtA in Enterococcus faecium and Enterococcus faecalis from Food-Producing Animals during 2008–2018 in Korea

  • Seok-Hyeon Na,
  • Dong-Chan Moon,
  • Mi-Hyun Kim,
  • Hee-Young Kang,
  • Su-Jeong Kim,
  • Ji-Hyun Choi,
  • Abraham-Fikru Mechesso,
  • Soon-Seek Yoon and
  • Suk-Kyung Lim

We aimed to investigate the presence of the phenicol–oxazolidinone resistance gene poxtA in linezolid-resistant enterococci from food-producing animals and analyze its molecular characteristics. We collected 3941 Enterococcus faecium and 5088 E...

(This article belongs to the Special Issue Antimicrobial Resistance and Molecular Tracing of Foodborne Pathogens)
  • Review
  • Open Access
21 Citations
8,839 Views
33 Pages

The COVID-19 pandemic, caused by a novel zoonotic coronavirus (CoV), SARS-CoV-2, has infected 46,182 million people, resulting in 1,197,026 deaths (as of 1 November 2020), with devastating and far-reaching impacts on economies and societies worldwide...

(This article belongs to the Special Issue COVID-19: Focusing on Epidemiologic, Virologic, and Clinical Studies)
  • Review
  • Open Access
34 Citations
8,250 Views
22 Pages

It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to underst...

(This article belongs to the Special Issue Microbe Electrode Electron Transfer: Understanding Interactions from the Enzyme to the Microbial Community Level)
  • Article
  • Open Access
10 Citations
4,160 Views
16 Pages

Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with an oxidation state independent from the substrate. The whole cell biocatalyst transfers the surplus of electrons to an e...

(This article belongs to the Special Issue Microbe Electrode Electron Transfer: Understanding Interactions from the Enzyme to the Microbial Community Level)
  • Article
  • Open Access
30 Citations
4,534 Views
16 Pages

Selected Rhizosphere Bacteria Help Tomato Plants Cope with Combined Phosphorus and Salt Stresses

  • Gylaine Vanissa Tchuisseu Tchakounté,
  • Beatrice Berger,
  • Sascha Patz,
  • Matthias Becker,
  • Henri Fankem,
  • Victor Désiré Taffouo and
  • Silke Ruppel

Plants are often challenged by multiple abiotic stresses simultaneously. The inoculation of beneficial bacteria is known to enhance plant growth under these stresses, such as phosphorus starvation or salt stress. Here, for the first time, we assessed...

(This article belongs to the Section Plant Microbe Interactions)
  • Article
  • Open Access
6 Citations
3,224 Views
17 Pages

E6/E7 Sequence Diversity of High-Risk Human Papillomaviruses in Two Geographically Isolated Populations of French Guiana

  • Mathieu Nacher,
  • Gersande Godefroy,
  • Valentin Dufit,
  • Maylis Douine,
  • Fatiha Najioullah,
  • Raymond Césaire,
  • Nadia Thomas,
  • Kinan Drak Alsibai,
  • Antoine Adenis and
  • Vincent Lacoste

Amerindian and Maroon populations of French Guiana have been living in isolation for generations and sexual networks remained mostly endogamous. The present study aimed to describe the phylogeny of E6 and E7 sequences of the most common high-risk HPV...

(This article belongs to the Section Molecular Microbiology and Immunology)
  • Article
  • Open Access
35 Citations
6,106 Views
17 Pages

Strain-Dependent Cheese Spoilage Potential of Clostridium tyrobutyricum

  • Lucija Podrzaj,
  • Johanna Burtscher,
  • Franziska Küller and
  • Konrad J. Domig

Clostridium tyrobutyricum, a Gram-positive, anaerobic, spore-forming bacterium, is considered as one of the main causative agents for spoilage of hard and semihard cheeses. Growth of C. tyrobutyricum in cheese is critically influenced by ripening tem...

(This article belongs to the Special Issue Beneficial and Detrimental Microorganisms Occurring in Fermented Foods)
  • Article
  • Open Access
7 Citations
6,399 Views
22 Pages

A commercially available microfluidics flow cell was utilized together with widefield fluorescence microscopy to evaluate the effects of disinfectants on bacterial strains. The flow cell’s inner surface supports the formation of biofilms of num...

(This article belongs to the Special Issue New Methods in Microbial Research)
  • Article
  • Open Access
5 Citations
3,811 Views
13 Pages

Bacteria-Derived Hemolysis-Related Genes Widely Exist in Scuticociliates

  • Jing Zhang,
  • Kai Chen,
  • Chuanqi Jiang,
  • Wentao Yang,
  • Siyu Gu,
  • Guangying Wang,
  • Yishan Lu,
  • Wei Miao and
  • Jie Xiong

Scuticociliatosis is an invasive external or systemic infection caused by ciliated protozoa, mainly those within the subclass Scuticociliatia (scuticociliates). Many scuticociliates are fish pathogens, including Miamiensis avidus, Philasterides dicen...

(This article belongs to the Section Molecular Microbiology and Immunology)
  • Review
  • Open Access
32 Citations
5,612 Views
20 Pages

Autochthonous Probiotics in Meat Products: Selection, Identification, and Their Use as Starter Culture

  • Paulo E. S. Munekata,
  • Mirian Pateiro,
  • Wangang Zhang,
  • Rubén Domínguez,
  • Lujuan Xing,
  • Elena Movilla Fierro and
  • José M. Lorenzo

The increasing demand for functional food is pushing the food industry to innovate the conventional and well-known foods. Producing functional foods, especially with probiotics in meat products, is an intricate and multistage task that involves: the...

(This article belongs to the Special Issue Microbiomes for the Sustainable Production of Safe and Secure Foods)
  • Article
  • Open Access
31 Citations
5,370 Views
25 Pages

Screening of FDA-Approved Drugs Using a 384-Well Plate-Based Biofilm Platform: The Case of Fingolimod

  • Shella Gilbert-Girard,
  • Kirsi Savijoki,
  • Jari Yli-Kauhaluoma and
  • Adyary Fallarero

In an effort to find new repurposed antibacterial compounds, we performed the screening of an FDA-approved compounds library against Staphylococcus aureus American Type Culture Collection (ATCC) 25923. Compounds were evaluated for their capacity to p...

(This article belongs to the Section Antimicrobial Agents and Resistance)
  • Review
  • Open Access
45 Citations
10,341 Views
31 Pages

Deciphering Streptococcal Biofilms

  • Puja Yadav,
  • Shalini Verma,
  • Richard Bauer,
  • Monika Kumari,
  • Meenakshi Dua,
  • Atul Kumar Johri,
  • Vikas Yadav and
  • Barbara Spellerberg

Streptococci are a diverse group of bacteria, which are mostly commensals but also cause a considerable proportion of life-threatening infections. They colonize many different host niches such as the oral cavity, the respiratory, gastrointestinal, an...

(This article belongs to the Section Biofilm)
  • Article
  • Open Access
61 Citations
6,844 Views
18 Pages

Phosphorus Reduces Negative Effects of Nitrogen Addition on Soil Microbial Communities and Functions

  • Zongwei Xia,
  • Jingyi Yang,
  • Changpeng Sang,
  • Xu Wang,
  • Lifei Sun,
  • Ping Jiang,
  • Chao Wang and
  • Edith Bai

Increased soil nitrogen (N) from atmospheric N deposition could change microbial communities and functions. However, the underlying mechanisms and whether soil phosphorus (P) status are responsible for these changes still have not been well explained...

(This article belongs to the Special Issue Microbial Community Response to Climate and Environmental Changes)
  • Article
  • Open Access
45 Citations
6,703 Views
16 Pages

Behind Taxonomic Variability: The Functional Redundancy in the Tick Microbiome

  • Agustín Estrada-Peña,
  • Alejandro Cabezas-Cruz and
  • Dasiel Obregón

The taxonomic composition and diversity of tick midgut microbiota have been extensively studied in different species of the genera Rhipicephalus, Ixodes, Amblyomma, Haemaphysalis, Hyalomma, Dermacentor, Argas and Ornithodoros, while the functional si...

(This article belongs to the Section Parasitology)
  • Article
  • Open Access
7 Citations
4,095 Views
11 Pages

The aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant Escherichia coli (QREC) strains with three different biofilm matrix compositions were in...

(This article belongs to the Special Issue Antimicrobial Resistance in the Food Production Chain )
  • Article
  • Open Access
22 Citations
5,383 Views
19 Pages

Phenotypic Characterization and Transformation Attempts Reveal Peculiar Traits of Xylella fastidiosa Subspecies pauca Strain De Donno

  • Giusy D’Attoma,
  • Massimiliano Morelli,
  • Leonardo De La Fuente,
  • Paul A. Cobine,
  • Maria Saponari,
  • Alessandra Alves de Souza,
  • Angelo De Stradis and
  • Pasquale Saldarelli

Xylella fastidiosa subsp. pauca strain De Donno has been recently identified as the causal agent of a severe disease affecting olive trees in a wide area of the Apulia Region (Italy). While insights on the genetics and epidemiology of this virulent s...

(This article belongs to the Special Issue Integrating Science on Xanthomonas and Xylella for Integrated Plant Disease Management)
  • Article
  • Open Access
32 Citations
5,526 Views
15 Pages

Anaplasma and Theileria Pathogens in Cattle of Lambwe Valley, Kenya: A Case for Pro-Active Surveillance in the Wildlife–Livestock Interface

  • Michael N. Okal,
  • Brenda Kisia Odhiambo,
  • Peter Otieno,
  • Joel L. Bargul,
  • Daniel Masiga,
  • Jandouwe Villinger and
  • Shewit Kalayou

Tick-borne pathogens (TBPs) are major constraints to livestock production and a threat to public health in Africa. This cross-sectional study investigated the risk of infection with TBPs in cattle of Lambwe Valley, Kenya. Blood samples of 680 zebu ca...

(This article belongs to the Special Issue Advance in Tick-Borne Diseases Research)
  • Article
  • Open Access
28 Citations
4,293 Views
11 Pages

The Acquisition of Multidrug-Resistant Bacteria in Patients Admitted to COVID-19 Intensive Care Units: A Monocentric Retrospective Case Control Study

  • Elisa G. Bogossian,
  • Fabio S. Taccone,
  • Antonio Izzi,
  • Nicolas Yin,
  • Alessandra Garufi,
  • Stephane Hublet,
  • Hassane Njimi,
  • Amedee Ego,
  • Julie Gorham and
  • David Grimaldi
  • + 5 authors

Whether the risk of multidrug-resistant bacteria (MDRB) acquisition in the intensive care unit (ICU) is modified by the COVID-19 crisis is unknown. In this single center case control study, we measured the rate of MDRB acquisition in patients admitte...

(This article belongs to the Special Issue Complex Infectious Issues in Critically Ill Patients)
  • Article
  • Open Access
29 Citations
4,229 Views
20 Pages

Intra- and Inter-Host Assessment of Bartonella Diversity with Focus on Non-Hematophagous Bats and Associated Ectoparasites from Brazil

  • Priscila Ikeda,
  • Jaire Marinho Torres,
  • Lívia Perles,
  • Elizabete Captivo Lourenço,
  • Heitor Miraglia Herrera,
  • Carina Elisei de Oliveira,
  • Rosangela Zacarias Machado and
  • Marcos Rogério André

The relationship among bats, ectoparasites and associated microorganisms is important to investigate how humans can become exposed to zoonotic agents. Even though the diversity of Bartonella spp. in bats and ectoparasites has been previously reported...

(This article belongs to the Section Parasitology)
  • Article
  • Open Access
20 Citations
6,012 Views
15 Pages

Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

  • Joanna Kwiecińska-Piróg,
  • Jana Przekwas,
  • Michał Majkut,
  • Krzysztof Skowron and
  • Eugenia Gospodarek-Komkowska

Chronic wound infections are difficult to manage because of the biofilm formation in the wound environment. New measures for eliminating infections are necessary to increase the chance of wound healing. Apitherapy may be the new solution. The aim of...

(This article belongs to the Special Issue Clinical Implications of Microbial Biofilm)
  • Review
  • Open Access
110 Citations
11,597 Views
19 Pages

Epstein–Barr Virus (EBV) contributes to the development of lymphoid and epithelial malignancies. While EBV’s latent phase is more commonly associated with EBV-associated malignancies, there is increasing evidence that EBV’s lytic phase plays a role i...

(This article belongs to the Special Issue Epstein–Barr Virus Infection and Associated Diseases)
  • Article
  • Open Access
32 Citations
4,144 Views
12 Pages

Antimicrobial Susceptibility and Association with Toxin Determinants in Clostridium perfringens Isolates from Chickens

  • Bai Wei,
  • Se-Yeoun Cha,
  • Jun-Feng Zhang,
  • Ke Shang,
  • Hae-Chul Park,
  • JeongWoo Kang,
  • Kwang-Jick Lee,
  • Min Kang and
  • Hyung-Kwan Jang

The aim of the present study was to investigate variation in antimicrobial resistance in Clostridium perfringens (C. perfringens) isolated from chickens after withdrawal of antimicrobial growth promoters (AGPs); and to investigate the correlation bet...

(This article belongs to the Section Antimicrobial Agents and Resistance)
  • Article
  • Open Access
18 Citations
4,531 Views
18 Pages

Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles of Burkholderia multivorans C1576, a Strain of Clinical Importance for Cystic Fibrosis

  • Lucrecia C. Terán,
  • Marco Distefano,
  • Barbara Bellich,
  • Sara Petrosino,
  • Paolo Bertoncin,
  • Paola Cescutti and
  • Daniele Sblattero

Biofilms are aggregates of microbial cells encased in a highly hydrated matrix made up of self-produced extracellular polymeric substances (EPS) which consist of polysaccharides, proteins, nucleic acids, and lipids. While biofilm matrix polysaccharid...

(This article belongs to the Section Biofilm)
  • Review
  • Open Access
49 Citations
13,645 Views
12 Pages

Autoimmune Gastritis and Gastric Microbiota

  • Laura Conti,
  • Bruno Annibale and
  • Edith Lahner

Autoimmune atrophic gastritis is an organ-specific immune-mediated condition characterized by atrophy of the oxyntic mucosa. Autoimmune atrophic gastritis (AIG) is characterized by a progressive loss of acid-secreting parietal cells leading to hypo-a...

(This article belongs to the Special Issue Microbial and Autoimmune Disease)
  • Article
  • Open Access
44 Citations
6,009 Views
21 Pages

Anaplasma phagocytophilum and Other Anaplasma spp. in Various Hosts in the Mnisi Community, Mpumalanga Province, South Africa

  • Agatha O. Kolo,
  • Nicola E. Collins,
  • Kelly A. Brayton,
  • Mamohale Chaisi,
  • Lucille Blumberg,
  • John Frean,
  • Cory A. Gall,
  • Jeanette M. Wentzel,
  • Samantha Wills-Berriman and
  • Marinda C. Oosthuizen
  • + 3 authors

DNA samples from 74 patients with non-malarial acute febrile illness (AFI), 282 rodents, 100 cattle, 56 dogs and 160 Rhipicephalus sanguineus ticks were screened for the presence of Anaplasma phagocytophilum DNA using a quantitative PCR (qPCR) assay...

(This article belongs to the Section Public Health Microbiology)
  • Article
  • Open Access
22 Citations
5,115 Views
15 Pages

Next-Generation Sequencing as a Tool to Detect Vaginal Microbiota Disturbances during Pregnancy

  • Agnieszka Sroka-Oleksiak,
  • Tomasz Gosiewski,
  • Wojciech Pabian,
  • Artur Gurgul,
  • Przemysław Kapusta,
  • Agnieszka H. Ludwig-Słomczyńska,
  • Paweł P. Wołkow and
  • Monika Brzychczy-Włoch

The physiological microbiota of the vagina is responsible for providing a protective barrier, but Some factors can disturb the balance in its composition. At that time, the amounts of the genus Lactobacillus decrease, which may lead to the developmen...

(This article belongs to the Section Medical Microbiology)
  • Communication
  • Open Access
12 Citations
3,041 Views
7 Pages

Performance of Population Pharmacokinetic Models in Predicting Polymyxin B Exposures

  • Vincent H. Tam,
  • Lawrence S. Lee,
  • Tat-Ming Ng,
  • Tze-Peng Lim,
  • Benjamin P. Z. Cherng,
  • Hafeez Adewusi,
  • Kim H. Hee,
  • Ying Ding,
  • Shimin Jasmine Chung and
  • David C. Lye
  • + 3 authors

Polymyxin B is the last line of defense in treating multidrug-resistant gram-negative bacterial infections. Dosing of polymyxin B is currently based on total body weight, and a substantial intersubject variability has been reported. We evaluated the...

(This article belongs to the Section Antimicrobial Agents and Resistance)
  • Article
  • Open Access
33 Citations
4,219 Views
17 Pages

Nutritional Conditions Modulate C. neoformans Extracellular Vesicles’ Capacity to Elicit Host Immune Response

  • Clara Luna Marina,
  • Pedro Henrique Bürgel,
  • Daniel Paiva Agostinho,
  • Daniel Zamith-Miranda,
  • Lucas de Oliveira Las-Casas,
  • Aldo Henrique Tavares,
  • Joshua Daniel Nosanchuk and
  • Anamelia Lorenzetti Bocca

Cryptococcus neoformans is a human pathogenic fungus that mainly afflicts immunocompromised patients. One of its virulence strategies is the production of extracellular vesicles (EVs), containing cargo with immunomodulatory properties. We evaluated E...

(This article belongs to the Special Issue Current Understanding of Cryptococcus and Cryptococcosis)
  • Communication
  • Open Access
16 Citations
3,707 Views
8 Pages

Emergence of Enterobacter cloacae Complex Co-Producing IMP-10 and CTX-M, and Klebsiella pneumoniae Producing VIM-1 in Clinical Isolates in Japan

  • Satoshi Nishida,
  • Naohisa Matsunaga,
  • Yuta Kamimura,
  • Shinobu Ishigaki,
  • Taiji Furukawa and
  • Yasuo Ono

Background: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat in healthcare settings worldwide. Objectives: We evaluated the presence of carbapenemase genes in CPE in a tertiary care university hospital in Tokyo, Japan. Methods:...

(This article belongs to the Special Issue Carbapenemase-producing Enterobacteriaceae)
  • Article
  • Open Access
16 Citations
4,619 Views
13 Pages

The Red Fox (Vulpes vulpes) as Sentinel for Tick-Borne Encephalitis Virus in Endemic and Non-Endemic Areas

  • Maja Haut,
  • Philipp Girl,
  • Beate Oswald,
  • Thomas Romig,
  • Anna Obiegala,
  • Gerhard Dobler and
  • Martin Pfeffer

Tick-borne encephalitis (TBE) is one of the most important viral zoonosis caused by a neurotropic arbovirus (TBEV). In Germany, TBE is classified as a notifiable disease with an average of 350 autochthonous human cases annually. The incidence-based r...

(This article belongs to the Special Issue Tick-Borne Encephalitis)
  • Article
  • Open Access
29 Citations
3,828 Views
13 Pages

Psychological Distress in Patients with Autoimmune Arthritis during the COVID-19 Induced Lockdown in Italy

  • Andrea Picchianti Diamanti,
  • Maria Sofia Cattaruzza,
  • Roberta Di Rosa,
  • Flavia Del Porto,
  • Simonetta Salemi,
  • Maria Laura Sorgi,
  • Luis Severino Martin Martin,
  • Alessandra Rai,
  • Dalila Iacono and
  • Bruno Laganà
  • + 2 authors

Lockdowns imposed by governments worldwide as a way to limit the spread of severe atypical respiratory syndrome-coronavirus-2 (SARS-CoV2) have had heavy psychological and economic consequences. Arthritis patients are a vulnerable population at an inc...

(This article belongs to the Special Issue Microbial and Autoimmune Disease)
  • Article
  • Open Access
15 Citations
3,615 Views
12 Pages

Cryptic HBV Replicative Activity Is Frequently Revealed in Anti-HBc-Positive/HBsAg-Negative Patients with HIV Infection by Highly Sensitive Molecular Assays, and Can Be Predicted by Integrating Classical and Novel Serological HBV Markers

  • Romina Salpini,
  • Vincenzo Malagnino,
  • Lorenzo Piermatteo,
  • Tiziana Mulas,
  • Mohammad Alkhatib,
  • Rossana Scutari,
  • Elisabetta Teti,
  • Carlotta Cerva,
  • Katia Yu La Rosa and
  • Valentina Svicher
  • + 12 authors

The anti-HBc-positive/HBsAg-negative status is frequent in HIV-infection and correlates with poor survival. Here, by highly-sensitive assays, we evaluate cryptic HBV replication and factors correlated with its detection in 81 anti-HBc-positive/HBsAg-...

(This article belongs to the Special Issue Updates on HBV Infection)
  • Article
  • Open Access
28 Citations
5,213 Views
18 Pages

Phenotypic Adaptation of Pseudomonas aeruginosa in the Presence of Siderophore-Antibiotic Conjugates during Epithelial Cell Infection

  • Quentin Perraud,
  • Paola Cantero,
  • Mathilde Munier,
  • Françoise Hoegy,
  • Nicolas Zill,
  • Véronique Gasser,
  • Gaëtan L. A. Mislin,
  • Laurence Ehret-Sabatier and
  • Isabelle J. Schalk

Iron acquisition pathways have often been considered to be gateways for the uptake of antibiotics into bacteria. Bacteria excrete chelators, called siderophores, to access iron. Antibiotic molecules can be covalently attached to siderophores for thei...

(This article belongs to the Special Issue Bacterial Stress Response Mechanisms: Signal Perception, Transduction and Adaptation)
  • Review
  • Open Access
25 Citations
4,393 Views
15 Pages

Phage Prevalence in the Human Urinary Tract—Current Knowledge and Therapeutic Implications

  • Maciej Żaczek,
  • Beata Weber-Dąbrowska,
  • Ryszard Międzybrodzki and
  • Andrzej Górski

Recent metagenomic analyses imply an immense abundance of phages in the human body. Samples collected from different sites (lungs, skin, oral cavity, intestines, ascitic fluid, and urine) reveal a generally greater number of phage particles than that...

(This article belongs to the Special Issue Bacteriophages and Their Components as Promising and Alternative Tools for the Control of Bacterial Pathogens)
  • Article
  • Open Access
9 Citations
3,982 Views
16 Pages

Evaluation of a New Topical Treatment for the Control of Cutaneous Leishmaniasis

  • Berenice Martínez-Salazar,
  • Vanessa Carregaro Pereira,
  • Yazmin Hauyon-La-Torre,
  • Ali Khamesipour and
  • Fabienne Tacchini-Cottier

Leishmania major (L. major) causes cutaneous leishmaniasis in the Old World. The infection mostly induces a localized lesion restricted to the sand fly bite. The costs and the side effects of current treatments render imperative the development of ne...

(This article belongs to the Special Issue Treatment of Leishmaniasis)
  • Article
  • Open Access
15 Citations
4,305 Views
15 Pages

Recently, we reported that cigarette smoking, and especially nicotine, increases susceptibility to mycobacterial infection and exacerbates inflammation in patients with Crohn’s disease (CD). The macrophagic response to Mycobacterium avium subsp...

(This article belongs to the Section Medical Microbiology)
  • Article
  • Open Access
11 Citations
4,894 Views
25 Pages

Aeromonas hydrophila RIT668 and Citrobacter portucalensis RIT669—Potential Zoonotic Pathogens Isolated from Spotted Turtles

  • Seema G. Thomas,
  • Milky Abajorga,
  • Maryah A. Glover,
  • Peter C. Wengert,
  • Anutthaman Parthasarathy,
  • Michael A. Savka,
  • Crista B. Wadsworth,
  • Paul A. Shipman and
  • André O. Hudson

Antimicrobial resistance (AMR) is one of the biggest challenges of the 21st century, and biofilm formation enables bacteria to resist antibiotic at much higher concentrations than planktonic cells. Earlier, we showed that the Gram-negative Aeromonas ...

(This article belongs to the Section Molecular Microbiology and Immunology)
  • Article
  • Open Access
5 Citations
3,870 Views
18 Pages

Transcriptomic Analysis of Staphylococcus xylosus in Solid Dairy Matrix Reveals an Aerobic Lifestyle Adapted to Rind

  • Sabine Leroy,
  • Sergine Even,
  • Pierre Micheau,
  • Anne de La Foye,
  • Valérie Laroute,
  • Yves Le Loir and
  • Régine Talon

Staphylococcus xylosus is found in the microbiota of traditional cheeses, particularly in the rind of soft smeared cheeses. Despite its frequency, the molecular mechanisms allowing the growth and adaptation of S. xylosus in dairy products are still p...

(This article belongs to the Section Food Microbiology)
  • Article
  • Open Access
9 Citations
6,679 Views
15 Pages

Clinical and Microbiological Efficacy of Pyrophosphate Containing Toothpaste: A Double-Blinded Placebo-Controlled Randomized Clinical Trial

  • Inpyo Hong,
  • Hyun Gee Lee,
  • Hye Lim Keum,
  • Myong Ji Kim,
  • Ui-Won Jung,
  • KiJung Kim,
  • Su Yeon Kim,
  • Taehun Park,
  • Hye-Jin Kim and
  • Jae-Kook Cha
  • + 3 authors

(1) Background: Dental calculus works as a niche wherein pathogenic bacteria proliferate in the oral cavity. Previous studies revealed the anticalculus activity of pyrophosphates, however there was no clinical study that evaluated microbiome changes...

(This article belongs to the Section Medical Microbiology)
  • Article
  • Open Access
19 Citations
3,854 Views
15 Pages

Disrupting Bordetella Immunosuppression Reveals a Role for Eosinophils in Coordinating the Adaptive Immune Response in the Respiratory Tract

  • Monica C. Gestal,
  • Uriel Blas-Machado,
  • Hannah M. Johnson,
  • Lily N. Rubin,
  • Kalyan K. Dewan,
  • Claire Bryant,
  • Michael Tiemeyer and
  • Eric T. Harvill

Recent findings revealed pivotal roles for eosinophils in protection against parasitic and viral infections, as well as modulation of adaptive immune responses in the gastric mucosa. However, the known effects of eosinophils within the respiratory tr...

(This article belongs to the Special Issue Infectious Diseases, New Approaches to Old Problems)
  • Article
  • Open Access
39 Citations
7,231 Views
18 Pages

Single Cell Oil Production by Oleaginous Yeasts Grown in Synthetic and Waste-Derived Volatile Fatty Acids

  • Sara Bettencourt,
  • Catarina Miranda,
  • Tatiana A. Pozdniakova,
  • Paula Sampaio,
  • Ricardo Franco-Duarte and
  • Célia Pais

Four yeast isolates from the species—Apiotrichum brassicae, Candida tropicalis, Metschnikowia pulcherrima, and Pichia kudriavzevii—previously selected by their oleaginous character and growth flexibility in different carbon sources, were...

(This article belongs to the Section Microbial Biotechnology)
  • Review
  • Open Access
50 Citations
15,320 Views
19 Pages

Prevention of New Respiratory Episodes in Children with Recurrent Respiratory Infections: An Expert Consensus Statement from the World Association of Infectious Diseases and Immunological Disorders (WAidid)

  • Susanna Esposito,
  • Marcus Herbert Jones,
  • Wojciech Feleszko,
  • José A. Ortega Martell,
  • Oana Falup-Pecurariu,
  • Natalia Geppe,
  • Federico Martinón-Torres,
  • Kun-Ling Shen,
  • Michael Roth and
  • Nicola Principi

In healthy infants and young children, the development of respiratory tract infections (RTIs) is extremely common. In this paper, we present an international consensus of the available approaches for the prevention of recurrent RTIs in children, incl...

(This article belongs to the Special Issue Respiratory Tract Infection in Children)
  • Article
  • Open Access
24 Citations
4,736 Views
22 Pages

Duodenal Metatranscriptomics to Define Human and Microbial Functional Alterations Associated with Severe Obesity: A Pilot Study

  • Ilaria Granata,
  • Carmela Nardelli,
  • Valeria D’Argenio,
  • Salvatore Tramontano,
  • Debora Compare,
  • Mario Rosario Guarracino,
  • Gerardo Nardone,
  • Vincenzo Pilone and
  • Lucia Sacchetti

Obesity is a multifactorial disorder, and the gut microbiome has been suggested to contribute to its onset. In order to better clarify the role of the microbiome in obesity, we evaluated the metatranscriptome in duodenal biopsies from a cohort of 23...

(This article belongs to the Special Issue The Human Gut Microbiome, Diets and Health)
  • Article
  • Open Access
2 Citations
3,512 Views
16 Pages

Proteomics and Lipidomics Investigations to Decipher the Behavior of Willaertia magna C2c Maky According to Different Culture Modes

  • Issam Hasni,
  • Nicholas Armstrong,
  • Philippe Decloquement,
  • Said Azza,
  • Anthony Fontanini,
  • Olivier Abbe,
  • Amina Cherif Louazani,
  • Sandrine Demanèche,
  • Eric Chabrière and
  • Bernard La Scola
  • + 1 author

Willaertia magna C2c Maky is a free-living amoeba that has demonstrated its ability to inhibit the intracellular multiplication of some Legionella pneumophila strains, which are pathogenic bacteria inhabiting the aquatic environment. The Amoeba, an i...

(This article belongs to the Section Microbial Biotechnology)
  • Article
  • Open Access
12 Citations
4,493 Views
12 Pages

Prevalence of Hepatitis B Virus Markers in Patients with Autoimmune Inflammatory Rheumatic Diseases in Italy

  • Marco Canzoni,
  • Massimo Marignani,
  • Maria Laura Sorgi,
  • Paola Begini,
  • Michela Ileen Biondo,
  • Sara Caporuscio,
  • Vincenzo Colonna,
  • Francesca Della Casa,
  • Paola Conigliaro and
  • Tommaso Stroffolini
  • + 10 authors

Chronic hepatitis B virus (HBV) infection may be reactivated by immunosuppressive drugs in patients with autoimmune inflammatory rheumatic diseases. This study evaluates HBV serum markers’ prevalence in rheumatic outpatients belonging to Spondy...

(This article belongs to the Special Issue Microbial and Autoimmune Disease)
  • Article
  • Open Access
21 Citations
5,097 Views
18 Pages

Genome-Scale Metabolic Modeling Reveals Metabolic Alterations of Multidrug-Resistant Acinetobacter baumannii in a Murine Bloodstream Infection Model

  • Jinxin Zhao,
  • Yan Zhu,
  • Jiru Han,
  • Yu-Wei Lin,
  • Michael Aichem,
  • Jiping Wang,
  • Ke Chen,
  • Tony Velkov,
  • Falk Schreiber and
  • Jian Li

Multidrug-resistant (MDR) Acinetobacter baumannii is a critical threat to human health globally. We constructed a genome-scale metabolic model iAB5075 for the hypervirulent, MDR A. baumannii strain AB5075. Predictions of nutrient utilization and gene...

(This article belongs to the Special Issue Genome-Scale Modeling of Microorganisms in the Real World)
  • Article
  • Open Access
1 Citations
2,827 Views
9 Pages

Detection of Parechovirus A1 with Monoclonal Antibody against Capsid Protein VP0

  • Ming-Hsiang Kung,
  • Ming-Wei Jan,
  • Jih-Jung Chen,
  • Yi-Chien Shieh and
  • Tsung-Hsien Chang

Parechovirus A (PeV-A; human parechovirus) causes mild infections and severe diseases such as neonatal sepsis, encephalitis, and cardiomyopathy in young children. Among the 19 types of PeV-A, PeV-A1 is the most common type of infection. We have previ...

(This article belongs to the Special Issue Molecular Epidemiology, Diagnostics and Management of Respiratory Virus Infections)
  • Article
  • Open Access
14 Citations
4,100 Views
19 Pages

Species-Specific Interactions of Bacillus Innocula and Arbuscular Mycorrhizal Fungi Symbiosis with Winter Wheat

  • Thomas I. Wilkes,
  • Douglas J. Warner,
  • Veronica Edmonds-Brown and
  • Keith G. Davies

Arbuscular mycorrhizal (AM) fungi establish close interactions with host plants, an estimated 80% of vascular plant species. The host plant receives additional soil bound nutrients that would otherwise not be available. Other components of the microb...

(This article belongs to the Section Environmental Microbiology)
  • Article
  • Open Access
37 Citations
4,986 Views
15 Pages

Persistent Activities of Extracellular Enzymes Adsorbed to Soil Minerals

  • Folasade K. Olagoke,
  • Klaus Kaiser,
  • Robert Mikutta,
  • Karsten Kalbitz and
  • Cordula Vogel

Adsorption of extracellular enzymes to soil minerals is assumed to protect them against degradation, while modifying their activities at the same time. However, the persistence of the activity of adsorbed enzymes remains poorly understood. Therefore,...

(This article belongs to the Special Issue Microorganisms – Minerals Interactions)
  • Review
  • Open Access
97 Citations
13,168 Views
30 Pages

Ochrobactrum species are non-enteric, Gram-negative organisms that are closely related to the genus Brucella. Since the designation of the genus in 1988, several distinct species have now been characterised and implicated as opportunistic pathogens i...

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Microorganisms - ISSN 2076-2607