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Microorganisms, Volume 8, Issue 11

November 2020 - 217 articles

Cover Story: Galleria mellonella larvae possess intrinsic autofluorescence that limits the use of fluorescently tagged bacteria for infection studies. To bypass this problem, a promoter probe vector expressing bioluminescence was constructed. Additionally, an RNA extraction protocol was optimized to study transcriptional gene levels during an in vivo infection. As proof of concept, the role of the different ribonucleotide reductase genes during a P. aeruginosa infection was studied. By using both methodologies, variable gene expression levels were successfully measured in the larvae at different time points. Both approaches enable a better study and monitoring of in vivo bacterial infections. View this paper
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Articles (217)

  • Article
  • Open Access
21 Citations
3,144 Views
10 Pages

Detection of the Phenicol–Oxazolidinone Resistance Gene poxtA in Enterococcus faecium and Enterococcus faecalis from Food-Producing Animals during 2008–2018 in Korea

  • Seok-Hyeon Na,
  • Dong-Chan Moon,
  • Mi-Hyun Kim,
  • Hee-Young Kang,
  • Su-Jeong Kim,
  • Ji-Hyun Choi,
  • Abraham-Fikru Mechesso,
  • Soon-Seek Yoon and
  • Suk-Kyung Lim

We aimed to investigate the presence of the phenicol–oxazolidinone resistance gene poxtA in linezolid-resistant enterococci from food-producing animals and analyze its molecular characteristics. We collected 3941 Enterococcus faecium and 5088 E...

  • Review
  • Open Access
20 Citations
7,942 Views
33 Pages

The COVID-19 pandemic, caused by a novel zoonotic coronavirus (CoV), SARS-CoV-2, has infected 46,182 million people, resulting in 1,197,026 deaths (as of 1 November 2020), with devastating and far-reaching impacts on economies and societies worldwide...

  • Review
  • Open Access
28 Citations
7,448 Views
22 Pages

It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to underst...

  • Article
  • Open Access
9 Citations
3,751 Views
16 Pages

Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with an oxidation state independent from the substrate. The whole cell biocatalyst transfers the surplus of electrons to an e...

  • Article
  • Open Access
24 Citations
4,103 Views
16 Pages

Selected Rhizosphere Bacteria Help Tomato Plants Cope with Combined Phosphorus and Salt Stresses

  • Gylaine Vanissa Tchuisseu Tchakounté,
  • Beatrice Berger,
  • Sascha Patz,
  • Matthias Becker,
  • Henri Fankem,
  • Victor Désiré Taffouo and
  • Silke Ruppel

Plants are often challenged by multiple abiotic stresses simultaneously. The inoculation of beneficial bacteria is known to enhance plant growth under these stresses, such as phosphorus starvation or salt stress. Here, for the first time, we assessed...

  • Article
  • Open Access
6 Citations
2,978 Views
17 Pages

E6/E7 Sequence Diversity of High-Risk Human Papillomaviruses in Two Geographically Isolated Populations of French Guiana

  • Mathieu Nacher,
  • Gersande Godefroy,
  • Valentin Dufit,
  • Maylis Douine,
  • Fatiha Najioullah,
  • Raymond Césaire,
  • Nadia Thomas,
  • Kinan Drak Alsibai,
  • Antoine Adenis and
  • Vincent Lacoste

Amerindian and Maroon populations of French Guiana have been living in isolation for generations and sexual networks remained mostly endogamous. The present study aimed to describe the phylogeny of E6 and E7 sequences of the most common high-risk HPV...

  • Article
  • Open Access
29 Citations
5,588 Views
17 Pages

Strain-Dependent Cheese Spoilage Potential of Clostridium tyrobutyricum

  • Lucija Podrzaj,
  • Johanna Burtscher,
  • Franziska Küller and
  • Konrad J. Domig

Clostridium tyrobutyricum, a Gram-positive, anaerobic, spore-forming bacterium, is considered as one of the main causative agents for spoilage of hard and semihard cheeses. Growth of C. tyrobutyricum in cheese is critically influenced by ripening tem...

  • Article
  • Open Access
6 Citations
5,197 Views
22 Pages

A commercially available microfluidics flow cell was utilized together with widefield fluorescence microscopy to evaluate the effects of disinfectants on bacterial strains. The flow cell’s inner surface supports the formation of biofilms of num...

  • Article
  • Open Access
4 Citations
3,437 Views
13 Pages

Bacteria-Derived Hemolysis-Related Genes Widely Exist in Scuticociliates

  • Jing Zhang,
  • Kai Chen,
  • Chuanqi Jiang,
  • Wentao Yang,
  • Siyu Gu,
  • Guangying Wang,
  • Yishan Lu,
  • Wei Miao and
  • Jie Xiong

Scuticociliatosis is an invasive external or systemic infection caused by ciliated protozoa, mainly those within the subclass Scuticociliatia (scuticociliates). Many scuticociliates are fish pathogens, including Miamiensis avidus, Philasterides dicen...

  • Review
  • Open Access
26 Citations
5,118 Views
20 Pages

Autochthonous Probiotics in Meat Products: Selection, Identification, and Their Use as Starter Culture

  • Paulo E. S. Munekata,
  • Mirian Pateiro,
  • Wangang Zhang,
  • Rubén Domínguez,
  • Lujuan Xing,
  • Elena Movilla Fierro and
  • José M. Lorenzo

The increasing demand for functional food is pushing the food industry to innovate the conventional and well-known foods. Producing functional foods, especially with probiotics in meat products, is an intricate and multistage task that involves: the...

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Microorganisms - ISSN 2076-2607