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Article

Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism

1
Department of Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany
2
Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
*
Author to whom correspondence should be addressed.
Current affiliation: Environmental Biotechnology, Centre for Applied Geosciences, University of Tübingen, Schnarrenbergstr. 94-96, 72074 Tübingen, Germany.
Microorganisms 2020, 8(11), 1843; https://doi.org/10.3390/microorganisms8111843
Received: 25 September 2020 / Revised: 19 November 2020 / Accepted: 21 November 2020 / Published: 23 November 2020
Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with an oxidation state independent from the substrate. The whole cell biocatalyst transfers the surplus of electrons to an electrode that can be used as a non-depletable electron acceptor. So far, anode-assisted fermentations were shown to provide high carbon efficiencies but low space-time yields. This study aimed at increasing space-time yields of an Escherichia coli-based anode-assisted fermentation of glucose to acetoin. The experiments build on an obligate respiratory strain, that was advanced using selective adaptation and targeted strain development. Several transfers under respiratory conditions led to point mutations in the pfl, aceF and rpoC gene. These mutations increased anoxic growth by three-fold. Furthermore, overexpression of genes encoding a synthetic electron transport chain to methylene blue increased the electron transfer rate by 2.45-fold. Overall, these measures and a medium optimization increased the space-time yield in an electrode-assisted fermentation by 3.6-fold. View Full-Text
Keywords: electro-fermentation; acetoin; methylene blue; bulk chemicals; metabolic engineering; Escherichia coli electro-fermentation; acetoin; methylene blue; bulk chemicals; metabolic engineering; Escherichia coli
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MDPI and ACS Style

Beblawy, S.; Philipp, L.-A.; Gescher, J. Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism. Microorganisms 2020, 8, 1843. https://doi.org/10.3390/microorganisms8111843

AMA Style

Beblawy S, Philipp L-A, Gescher J. Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism. Microorganisms. 2020; 8(11):1843. https://doi.org/10.3390/microorganisms8111843

Chicago/Turabian Style

Beblawy, Sebastian, Laura-Alina Philipp, and Johannes Gescher. 2020. "Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism" Microorganisms 8, no. 11: 1843. https://doi.org/10.3390/microorganisms8111843

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