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Pathogens, Volume 9, Issue 3

March 2020 - 88 articles

Cover Story: The hepatitis E virus (HEV) causes acute and chronic hepatitis in humans. Investigation into HEV replication is hampered by the lack of efficient cell culture systems and tools for site-directed mutagenesis. We developed the first plasmid-based reverse genetics system for a HEV strain that efficiently rescued the infectious virus without the need for in vitro RNA transcription. The cotransfection of T7 RNA polymerase-expressing BSR/T7 cells with one plasmid encoding the full-length viral genome and two helper plasmids encoding vaccinia virus capping enzymes resulted in the production of infectious HEV, which could be passaged on A549/D3 cells. The parental and recombinant virus exhibited similar replication kinetics. An additional restriction enzyme site could be introduced into the virus genome of the progeny virus, indicating that the system is suitable for site-directed mutagenesis. View this paper
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Articles (88)

  • Feature Paper
  • Article
  • Open Access
3 Citations
5,058 Views
11 Pages

The disease-associated prion protein (PrPSc) has the ability to seed the conformational conversion of normal prion proteins into the amyloid fibril form. This prion seeding activity can be measured using an in vitro amplification assay termed real-ti...

  • Article
  • Open Access
5 Citations
4,648 Views
18 Pages

Human Papillomavirus E6/E7 Expression in Preeclampsia-Affected Placentae

  • Ashley L. Reily-Bell,
  • Amanda Fisher,
  • Bryony Harrison,
  • Sara Bowie,
  • Sankalita Ray,
  • Mary Hawkes,
  • Lyn M. Wise,
  • Ryuji Fukuzawa,
  • Erin C. Macaulay and
  • Celia J. Devenish
  • + 2 authors

Whether HPV is causative of pregnancy complications is uncertain. E6 and E7 affect functions underling preeclampsia (PET) in cultured trophoblasts, but whether E6 and E7 is produced in the placenta is uncertain. Here, we investigated whether E6/E7 wa...

  • Article
  • Open Access
169 Citations
29,477 Views
12 Pages

Evolutionary Trajectory for the Emergence of Novel Coronavirus SARS-CoV-2

  • Saif ur Rehman,
  • Laiba Shafique,
  • Awais Ihsan and
  • Qingyou Liu

Over the last two decades, the world experienced three outbreaks of coronaviruses with elevated morbidity rates. Currently, the global community is facing emerging virus SARS-CoV-2 belonging to Betacoronavirus, which appears to be more transmissible...

  • Article
  • Open Access
36 Citations
6,180 Views
11 Pages

This paper was aimed to characterize clinical signs and pathomorphological lesions in twenty-two pigs, infected intranasally by different doses of African swine fever virus (Pol18_28298_O111), isolated during the outbreak in a pig farm that occurred...

  • Article
  • Open Access
78 Citations
8,700 Views
15 Pages

Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus

  • Abdelazeem M. Algammal,
  • Mohamed Fathi Mohamed,
  • Basma A. Tawfiek,
  • Wael N. Hozzein,
  • Waleed M. El Kazzaz and
  • Mahmoud Mabrok

Motile Aeromonas septicemia is a common bacterial disease that affects Oreochromis niloticus and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of Aeromonas hydro...

  • Article
  • Open Access
10 Citations
4,585 Views
10 Pages

Characterization of the Humoral Immune Response to Porcine Epidemic Diarrhea Virus Infection under Experimental and Field Conditions Using an AlphaLISA Platform

  • Kay Kimpston-Burkgren,
  • Juan Carlos Mora-Díaz,
  • Philippe Roby,
  • Jordan Bjustrom-Kraft,
  • Rodger Main,
  • Roger Bosse and
  • Luis Gabriel Giménez-Lirola

Coronavirus infections are a continuous threat raised time and again. With the recent emergence of novel virulent strains, these viruses can have a large impact on human and animal health. Porcine epidemic diarrhea (PED) is considered to be a reemerg...

  • Review
  • Open Access
81 Citations
19,174 Views
21 Pages

Toxoplasma gondii is a ubiquitous parasitic protist found in a wide variety of hosts, including a large proportion of the human population. Beyond an acute phase which is generally self-limited in immunocompetent individuals, the ability of the paras...

  • Article
  • Open Access
10 Citations
4,148 Views
12 Pages

Response of Human Neutrophil Granulocytes to the Hyphae of the Emerging Fungal Pathogen Curvularia lunata

  • Eszter Judit Tóth,
  • Mónika Varga,
  • Miklós Takó,
  • Mónika Homa,
  • Olivér Jáger,
  • Edit Hermesz,
  • Hajnalka Orvos,
  • Gábor Nagy,
  • Csaba Vágvölgyi and
  • Tamás Papp

Curvularia lunata is an ascomycete filamentous fungus causing local and invasive phaeohyphomycoses in both immunocompromised and immunocompetent patients. Neutrophils are crucial participants of the first line host defense against fungal infections....

  • Article
  • Open Access
78 Citations
8,657 Views
17 Pages

Fusarium species are common pathogens on maize and reduce the product quality through contamination with mycotoxins thus jeopardizing safety of both animal feed and human food products. Monitoring of Fusarium infected maize ears and stalks was conduc...

  • Article
  • Open Access
44 Citations
12,873 Views
18 Pages

Etiology and Symptoms of Maize Leaf Spot Caused by Bipolaris spp. in Sichuan, China

  • Xiaofang Sun,
  • Xiaobo Qi,
  • Wei Wang,
  • Xuan Liu,
  • Henan Zhao,
  • Cuiping Wu,
  • Xiaoli Chang,
  • Min Zhang,
  • Huabao Chen and
  • Guoshu Gong

Many species of the genus Bipolaris are important plant pathogens and often cause leaf spot, root rot, and seedling blight in an extremely wide range of hosts around the world. In recent years, maize leaf spot caused by Bipolaris species has frequent...

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Pathogens - ISSN 2076-0817