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Pathogens, Volume 14, Issue 10

October 2025 - 115 articles

Cover Story: Giant viruses are remarkably complex, featuring an outer protein shell built from major capsid proteins (MCPs) anchored onto an internal lipid membrane. During assembly, these viruses must recruit membrane material from the host cells into the growing particle—a process that has remained poorly understood. Our study provides the first mechanistic insight into how the MCP of the giant virus PBCV-1 interacts with lipid membranes. We reveal that membrane charge and curvature jointly guide MCP recruitment: negatively charged lipids enhance electrostatic attraction, while the membrane shape influences protein organization. By combining molecular experiments with computational simulations, we uncover how both electrostatics and lipid structure together orchestrate viral assembly—opening a window into how giant viruses recruit membranes to build their complex architectures. View this paper
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Articles (115)

  • Article
  • Open Access
369 Views
18 Pages

LINC1467 Activates the IPO8–p65 Axis to Restrict Hand, Foot, and Mouth Disease Virus Replication

  • Xiaokui Zhang,
  • Jinwei Li,
  • Li Ding,
  • Jihong Zhang,
  • Fan Yang,
  • Yonghan Luo and
  • Wei Chen

21 October 2025

Hand–foot–mouth disease (HFMD), primarily caused by human enteroviruses (EVs), poses a public health challenge, particularly among infants, due to a lack of effective therapies. Elucidating the molecular interplay between EVs and the host...

  • Article
  • Open Access
302 Views
14 Pages

Influence of Incubation Time for Viability Assessment of Ascaris suum Eggs—Attempts to Optimise the Parasitological Examination

  • Radosław Badziak,
  • Jolanta Zdybel,
  • Zbigniew Osiński,
  • Ewa Bilska-Zając,
  • Jacek Karamon,
  • Jacek Sroka,
  • Marta Skubida,
  • Angelina Wójcik-Fatla and
  • Tomasz Cencek

21 October 2025

The aim of this study was to determine the optimal incubation time for accurate assessment of Ascaris suum eggs viability, an important step in improving parasitological diagnostics. The experiment used A. suum eggs collected from three sources: adul...

  • Article
  • Open Access
309 Views
10 Pages

Effects of a Synthetic Isoquinoline Derivative Against Ichthyophthirius multifiliis In Vivo and In Vitro in Grass Carp (Ctenopharyngodon idella)

  • Xianqi Peng,
  • Xialian Bu,
  • Weisong Ma,
  • Jinbiao Jiao,
  • Xiaohong Huang,
  • Yu Zhao,
  • Jian Zhu,
  • Lei Huang,
  • Jing Chen and
  • A’qin Zheng
  • + 2 authors

21 October 2025

White spot disease, caused by the parasitic Ichthyophthirius multifiliis, induces mortality exceeding 80% in intensive aquaculture systems, resulting in global annual losses of >$1 billion. Current chemical controls (e.g., formaldehyde) face envir...

  • Article
  • Open Access
327 Views
17 Pages

21 October 2025

Leishmania major is a unicellular protozoan that causes cutaneous leishmaniasis in mammals and is mainly transmitted by the sand fly Phlebotomus papatasi. However, the contribution of microRNAs (miRNAs) and protein-coding genes to its pathogenic mech...

  • Case Report
  • Open Access
506 Views
14 Pages

Pulmonary Tuberculosis in the 19th Century: A Historical Case Study of Dr. Șerban Eminovici, Romanian Physician and Brother of Poet Mihai Eminescu

  • Andrei Ionut Cucu,
  • Catalin M. Buzduga,
  • Navena Widulin,
  • Alexandru Nemtoi,
  • Amelian Madalin Bobu,
  • Claudia Florida Costea,
  • Roxana Filip,
  • Vlad Porumb,
  • Anca Petruta Morosan and
  • Alexandru Carauleanu
  • + 5 authors

21 October 2025

Background: In the 19th century, pulmonary tuberculosis was the leading cause of death in Europe, responsible for up to one-quarter of all mortality. Before Robert Koch’s discovery of the tubercle bacillus in 1882 and the advent of effective th...

  • Article
  • Open Access
300 Views
17 Pages

21 October 2025

Background: Identifying optimal housekeeping genes is essential to accurately quantify gene expression dynamics across the 18th day (male and female begin to pair) and the 23rd day (female begin to sex mature) post infection of Schistosoma japonicum,...

  • Article
  • Open Access
356 Views
20 Pages

Specificity of Primers and Probes for Molecular Diagnosis of Leishmania (Leishmania) chagasi in Dogs and Wild Animals

  • Giovanna Zandonadi Haber,
  • Leila Dias da Costa,
  • Erick Bruno Monteiro Costa,
  • Railton Farias Araújo,
  • Tainá Negreiros de Souza,
  • Luciana do Rêgo Lima Queiroz,
  • Bruno Tardelli Nunes Diniz,
  • Edivaldo Costa Sousa Junior,
  • Lívia Carício Martis and
  • Patrícia Karla Santos Ramos
  • + 1 author

21 October 2025

Molecular tools, especially real-time polymerase chain reaction (qPCR), are relevant tools for laboratory diagnosis due to their sensitivity, specificity, rapid results, and ability to quantify parasite load. This study evaluated the specificity of t...

  • Article
  • Open Access
476 Views
12 Pages

Risk Factors and Clinical Outcomes of Invasive Fungal Infections in Patients with Severe COVID-19: A Case–Control Study

  • Nosheen Nasir,
  • Syed Abbas Moazzam Kazmi,
  • Joveria Farooqi,
  • Muhammad Irfan and
  • Kauser Jabeen

21 October 2025

Background: Invasive fungal infections (IFIs) in patients with COVID-19 contribute to significant morbidity and mortality, with reported incidence between 5% and 26.7%. COVID-19-associated pulmonary aspergillosis (CAPA), candidiasis (CAC), mucormycos...

  • Systematic Review
  • Open Access
690 Views
15 Pages

20 October 2025

Introduction: Due to the limited therapeutic options for patients with Acinetobacter baumannii complex infections, a new combination antimicrobial agent, sulbactam–durlobactam, has been developed. In this systematic review, we evaluated the ava...

  • Article
  • Open Access
525 Views
17 Pages

Fungus Among Us: An 8-Year Retrospective Study of Fungal Infections at a Tertiary-Care Hospital in Bucharest, Romania

  • Alina Maria Borcan,
  • Mihaela-Cristina Olariu,
  • Teodora Gabriela Anghel,
  • Elena Rotaru,
  • Bianca Secuiu,
  • Madalina Simoiu,
  • Narcis Copca,
  • Dragos Cretoiu and
  • Laura Georgiana Caravia

20 October 2025

Despite the devastating consequences of fungal disease, research struggles to catch up to present needs. This study aims to give a broad perspective on the situation, investigating patterns and distribution of fungal pathogens and monitoring trends o...

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Pathogens - ISSN 2076-0817