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Pathogens, Volume 9, Issue 3

March 2020 - 88 articles

Cover Story: The hepatitis E virus (HEV) causes acute and chronic hepatitis in humans. Investigation into HEV replication is hampered by the lack of efficient cell culture systems and tools for site-directed mutagenesis. We developed the first plasmid-based reverse genetics system for a HEV strain that efficiently rescued the infectious virus without the need for in vitro RNA transcription. The cotransfection of T7 RNA polymerase-expressing BSR/T7 cells with one plasmid encoding the full-length viral genome and two helper plasmids encoding vaccinia virus capping enzymes resulted in the production of infectious HEV, which could be passaged on A549/D3 cells. The parental and recombinant virus exhibited similar replication kinetics. An additional restriction enzyme site could be introduced into the virus genome of the progeny virus, indicating that the system is suitable for site-directed mutagenesis. View this paper
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Articles (88)

  • Article
  • Open Access
18 Citations
7,381 Views
19 Pages

Host-Parasite Interaction between Parasitic Cymothoid Ceratothoa oestroides and Its Host, Farmed European Sea Bass (Dicentrarchus labrax)

  • Ivona Mladineo,
  • Jerko Hrabar,
  • Olja Vidjak,
  • Ivana Bočina,
  • Slavica Čolak,
  • Pantelis Katharios,
  • Maria Chiara Cascarano,
  • Kleoniki Keklikoglou,
  • Donatella Volpatti and
  • Paola Beraldo

Parasitic isopod Ceratothoa oestroides (Cymothoidea, Isopoda) is a common and generalist buccal cavity-dweller in marine fish, recognised for its detrimental effect in fingerling and juvenile farmed European sea bass (Dicentrarchus labrax). Although...

  • Review
  • Open Access
539 Citations
147,259 Views
14 Pages

SARS-CoV-2 and Coronavirus Disease 2019: What We Know So Far

  • Firas A. Rabi,
  • Mazhar S. Al Zoubi,
  • Ghena A. Kasasbeh,
  • Dunia M. Salameh and
  • Amjad D. Al-Nasser

In December 2019, a cluster of fatal pneumonia cases presented in Wuhan, China. They were caused by a previously unknown coronavirus. All patients had been associated with the Wuhan Wholefood market, where seafood and live animals are sold. The virus...

  • Article
  • Open Access
18 Citations
6,126 Views
16 Pages

Cladobotryum dendroides, which causes cobweb disease in edible mushrooms, is one of the major fungal pathogens. Our previous studies focused on the genetic and morphological characterization of this fungus, as well as its pathogenicity and the identi...

  • Article
  • Open Access
34 Citations
6,185 Views
20 Pages

Tomato bacterial wilt caused by Ralstonia solanacearum (RS) is one of the most devastating soil-borne diseases, and compost is to be considered as a resource-saving and environment-friendly measure to control the disease. Herein, a pot experiment was...

  • Brief Report
  • Open Access
13 Citations
3,808 Views
7 Pages

Commensal Neisseria Are Shared between Sexual Partners: Implications for Gonococcal and Meningococcal Antimicrobial Resistance

  • Christophe Van Dijck,
  • Jolein G. E. Laumen,
  • Sheeba S. Manoharan-Basil and
  • Chris Kenyon

Antimicrobial resistance in pathogenic Neisseria parallels reduced antimicrobial susceptibility in commensal Neisseria in certain populations, like men who have sex with men (MSM). Although this reduced susceptibility can be a consequence of frequent...

  • Review
  • Open Access
19 Citations
6,035 Views
19 Pages

Molecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops

  • Yi Cheng,
  • Xiaoyu Tang,
  • Chunsheng Gao,
  • Zhimin Li,
  • Jia Chen,
  • Litao Guo,
  • Tuhong Wang and
  • Jianping Xu

Bast fibers and products derived from them are undergoing a resurgence in demand in the global market. However, fungal diseases have become an important factor limiting their yield and quality, causing devastating consequences for the production of b...

  • Article
  • Open Access
40 Citations
6,375 Views
10 Pages

High Human Papillomavirus DNA loads in Inflammatory Middle Ear Diseases

  • Nicola Malagutti,
  • John Charles Rotondo,
  • Luca Cerritelli,
  • Claudio Melchiorri,
  • Monica De Mattei,
  • Rita Selvatici,
  • Lucia Oton-Gonzalez,
  • Francesco Stomeo,
  • Manuela Mazzoli and
  • Fernanda Martini
  • + 4 authors

Background. Previous studies reported human papillomaviruses (HPVs) in middle ear tumors, whereas these viruses have been poorly investigated in chronic inflammatory middle ear diseases. We investigated HPVs in non-tumor middle ear diseases, includin...

  • Review
  • Open Access
108 Citations
23,386 Views
17 Pages

Recent Advances in Diagnostic Approaches for Epstein–Barr Virus

  • Mai Abdel Haleem Abusalah,
  • Siew Hua Gan,
  • Mohammad A. I. Al-Hatamleh,
  • Ahmad Adebayo Irekeola,
  • Rafidah Hanim Shueb and
  • Chan Yean Yean

Epstein–Barr virus (EBV) is the causative agent of many diseases including infectious mononucleosis (IM), and it is associated with different subtypes of lymphoma, sarcoma and carcinoma such as Hodgkin’s lymphoma, non-Hodgkin’s lymp...

  • Review
  • Open Access
19 Citations
12,090 Views
9 Pages

Complex Cell Type-Specific Roles of Autophagy in Liver Fibrosis and Cirrhosis

  • Tzu-Min Hung,
  • Chih-Chiang Hsiao,
  • Chih-Wen Lin and
  • Po-Huang Lee

The lysosomal degradation pathway, or autophagy, plays a fundamental role in cellular, tissue, and organismal homeostasis. A correlation between dysregulated autophagy and liver fibrosis (including end-stage disease, cirrhosis) is well-established. H...

  • Article
  • Open Access
7 Citations
3,287 Views
11 Pages

Combined Amoebicidal Effect of Atorvastatin and Commercial Eye Drops against Acanthamoeba castellanii Neff: In Vitro Assay Based on Mixture Design

  • Ines Sifaoui,
  • Eulalia Capote Yanes,
  • María Reyes-Batlle,
  • Rubén L. Rodríguez-Expósito,
  • José E. Piñero and
  • Jacob Lorenzo-Morales

The establishment of an effective therapeutic agent against Acanthamoeba keratitis (AK), remains until present, an issue to be solved due to the existence of a cyst stage in the life cycle of Acanthamoeba. Moreover, the effectiveness of the current s...

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Pathogens - ISSN 2076-0817