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Article

Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus

1
Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
2
National Institute of Oceanography and Fisheries, Suez 43511, Egypt
3
Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
4
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt
5
Botany Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt
6
Department of Fish Diseases and Management, Faculty of Veterinary Medicine Suez Canal University, Ismailia 41522, Egypt
7
Fish Infectious Diseases Research Unit (FID RU), Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(3), 238; https://doi.org/10.3390/pathogens9030238
Received: 1 March 2020 / Revised: 16 March 2020 / Accepted: 20 March 2020 / Published: 22 March 2020
(This article belongs to the Special Issue Animal Parasitic Diseases)
Motile Aeromonas septicemia is a common bacterial disease that affects Oreochromis niloticus and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of Aeromonas hydrophila complex, a total of 250 tilapia (Oreochromis niloticus) were collected randomly from 10 private tilapia farms (25 fish/farm) at El-Sharkia Governorate, Egypt. The collected fish were subjected to clinical and bacteriological examinations. The majority of infected fish displayed ulcerative necrosis, exophthalmia, and internal signs of hemorrhagic septicemia. The prevalence of A. hydrophia complex was 13.2%, where the liver was the most predominant affected organ (54.1%). Polymerase chain reaction (PCR) was used to verify the identification of A. hydrophila complex using one set of primers targeting gyrB as well as the detection of virulent genes (aerA, alt, and ahp). All isolates were positive for the gyrB-conserved gene and harbored aerA and alt virulence genes. However, none of those isolates were positive for the ahp gene. The antimicrobial sensitivity was carried out, where the recovered strains were completely sensitive to ciprofloxacin and highly resistant to amoxicillin. All retrieved strains showed the same phenotypic characteristics and were identical based on the restriction fragment length polymorphism (RFLP). Experimentally challenged fish presented a high mortality rate (76.67%) and showed typical signs as in naturally infected ones. In conclusion, the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of A. hydrophila complex. RFLP is a fundamental tool for monitoring the biodiversity among all retrieved strains of A. hydrophia. View Full-Text
Keywords: A. hydrophila complex; O. niloticus; PCR; RFLP; antibiogram A. hydrophila complex; O. niloticus; PCR; RFLP; antibiogram
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MDPI and ACS Style

Algammal, A.M.; Mohamed, M.F.; Tawfiek, B.A.; Hozzein, W.N.; El Kazzaz, W.M.; Mabrok, M. Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus. Pathogens 2020, 9, 238. https://doi.org/10.3390/pathogens9030238

AMA Style

Algammal AM, Mohamed MF, Tawfiek BA, Hozzein WN, El Kazzaz WM, Mabrok M. Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus. Pathogens. 2020; 9(3):238. https://doi.org/10.3390/pathogens9030238

Chicago/Turabian Style

Algammal, Abdelazeem M., Mohamed Fathi Mohamed, Basma A. Tawfiek, Wael N. Hozzein, Waleed M. El Kazzaz, and Mahmoud Mabrok. 2020. "Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus" Pathogens 9, no. 3: 238. https://doi.org/10.3390/pathogens9030238

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